Introduction: Hemotropic microorganisms, such as Trypanosoma spp., affect many animals, including water buffaloes. Some developing countries have reported trypanocide resistance to Diminazene Aceturate. This study aimed to describe the presence of resistance associated with trypanocide treatment in Colombian water buffaloes. Materials and methods: In this cross-sectional study, 202 individuals were sampled from an endemic area in Colombia. The molecular technique quantitative polymerase chain reaction (qPCR) was performed targeting the 18S gene for Trypanosoma spp., and phylogenetic analysis based on the ITS1 region was performed. Results: Sixty-one animals (30.2%) were positive for Trypanosoma spp. and fifty-two sequences were completely analyzed. Of the sequences, 76.9% (40/ 52) were compatible with T. vivax and 23.1% (12/ 52) with T. theileri; the obtained results did not show homologous sequences for T. evansi. Subsequently, positive animals were treated with Diminazene Aceturate at the recommended dose (7 mg/kg), and a final qPCR was performed one month after the end of the treatment. All animals were negative for Trypanosoma spp. after treatment with the drug. Discussion: Although no resistance to the drug was detected in the animals included in the study, it is necessary to implement continuous surveillance of trypanocidal drug resistance to verify the circulation of resistant strains in Colombia and endemic areas in other countries.
Introduction. Corticobasal syndrome (CBS) is a neurodegenerative disorder with distinctive motor and cognitive alterations. Objective. To synthesize the scientific literature on CBS, its neuroimaging report and neuropsychological profile. Methods. was structured as a subject review, where a search of scientific articles in indexed journals was carried out. Results. highlighted that cognitive dysfunction is characterized by apraxias, aphasias and sometimes executive dysfunction, it is also found that motor symptoms tend to intensify with the progression of the disease. Regarding neuroimaging, the main atrophy and hypometabolism evidenced is presented in frontal, temporal, parietal and striate nuclei. Discussion. These results offer comprehensive framework for understanding the development of CBS, providing a reference point for differential diagnosis, due to the heterogeneity of the underlying neuropathological diseases, being Corticobasal Dementia, the most common, not the only one. Conclusions. A treatment is evidenced, with pharmacological and non-pharmacological is mainly symptomatic that alleviate the impact on the daily life of patients with CBS, but of which future research is needed.
OBJECTIVE:To evaluate periapical healing at a 6-month follow-up after single-visit endodontic treatment using 3 commonly employed sealers-zinc oxide-eugenol (C-Gross), epoxy resin-based (AH Plus), and calcium silicate-based (Bio-C Sealer)-assessed through 3-dimensional cone-beam computed tomography (CBCT) volumetric analysis. METHODS:A comparative, parallel, non-randomised, double-blinded clinical design was used. Seventy-five patients with pulp necrosis and chronic apical periodontitis were enrolled; 70 completed follow-up (AH Plus:n=25; Bio-C: n=23; C-Gross: n=22). Treatments were performed by 10 supervised postgraduate operators following a standardised protocol. Preoperative and 6-month CBCT scans were segmented by 2 calibrated, blinded radiologists (κ = 0.90). Lesion volumes were obtained using manual boundary tracing and automated 3D reconstruction. Intragroup and intergroup comparisons were evaluated using non-parametric statistics. RESULTS:All 3 sealers showed significant intragroup reductions in lesion volume over 6 months (P < .001). Median volumes decreased from 81.0 to 15.0 mm3 (AH Plus), 63.0 to 12.0 mm3 (Bio-C), and 36.0 to 4.5 mm3 (C-Gross). Percentage reductions were similarly high across groups (median 79.7%-84.9%). No significant differences were observed among sealers for absolute volumetric change (P=.096) or percentage reduction (P=.912). CONCLUSIONS:Periapical healing was comparable across AH Plus: n=25; Bio-C: n=23; C-Gross: n=22. When disinfection, 3-dimensional obturation, and coronal sealing are optimally achieved, sealer composition has minimal influence on tissue repair. Healing appears primarily host-driven, governed by inflammatory resolution and bone remodelling rather than sealer-derived bioactivity.
OBJECTIVE:This retrospective cohort study aimed to (i) determine the prevalence of external cervical resorption (ECR) in a Colombian subpopulation; (ii) assess the long-term impact of treatment on tooth survival, success and failure according to ECR reactivation monitored by digital periapical radiography (DPR) and cone-beam computed tomography (CBCT); and (iii) identify possible predictive factors influencing treatment success. METHODS:The DPR and CBCT images of 5934 patients (2745 females and 3189 males) collected between January 2012 and October 2017 were examined for ECR lesions. Only teeth treated surgically via an external approach and followed for a minimum of 2 years were included in the outcome analysis. Lesions were classified using the Heithersay and Patel systems. Survival and recurrence were analysed using the Kaplan-Meier estimates and Cox proportional hazards regression model (P ≤ .05). RESULTS:The prevalence of ECR was 1.5% (94 patients, 97 teeth), with rates of 0.67% in females and 0.91% in males (P ≥ .05). Eighty-one patients each contributed 1 tooth to the outcome analysis. The overall survival rate was 77.8% at a median follow-up of 5.6 years. The overall success rates were 61.7% by DPR and 49.3% by CBCT, with no significant difference between methods (P ≥ .05). Lower ECR severity was associated with higher success in both classification systems. Furthermore, trauma, bruxism, and the use of Biodentine® were significantly associated with either the reactivation or non-reactivation of ECR lesions. CONCLUSION:The prevalence of ECR was low in this Colombian subpopulation, predominantly affecting maxillary and mandibular incisors, as well as maxillary canines. The CBCT was more sensitive than DPR for detecting ECR reactivation. Both survival and long-term treatment success were higher when ECR severity was lower. Notably, the critical time points for survival and reactivation were 2 and 3 years post-treatment, respectively.
Hypomineralised second primary molar (HSPM) is a developmental enamel defect whose aetiology remains incompletely understood. This study aimed to identify prenatal and birth-related risk factors associated with HSPM in children from a prospective cohort. This study was nested within the Araraquara Cohort. Of the 1,089 children considered potentially eligible for the dental assessment, 303 underwent clinical examination (27.8
