The Government of Saskatchewan (French: Gouvernement de la Saskatchewan) refers to the provincial government of the province of Saskatchewan. Its powers and structure are set out in the Constitution Act, 1867.In modern Canadian use, the term "government" referred broadly to the cabinet of the day (formally the Executive Council of Saskatchewan), elected from the Legislative Assembly of Saskatchewan and the non-political staff within each provincial department or agency – that is, the civil service.The Province of Saskatchewan is governed by a unicameral legislature, the Legislative Assembly of Saskatchewan, which operates in the Westminster system of government. The political party that wins the largest number of seats in the legislature normally forms the government, and the party's leader becomes premier of the province, i.e., the head of the government..
Many countries worldwide, particularly those in sub-Saharan Africa, suffer from malaria burden, both mortality and morbidity. Multiple funding agencies are investing to improve the health of populations. The aim of this study was to assess the impact of malaria funding on mortality and morbidity in children under 5 years of age over a 15-year period. A retrospective study was conducted from 2009 to 2023 across the country. Analysis was based on a secondary analysis of routine data from health facilities via DHIS2. Financial data was assembled from the malaria programme and health accounts. Between 2009 and 2023, Burkina Faso’s population grew by 50.7
Abstract Background In the absence of structured donor registries, social media platforms have become a dominant mechanism for blood donor recruitment in many low-resource settings. However, the implications of this shift for transfusion timeliness and system reliability remain unclear. Objective To evaluate the impact of social media–sourced donors on transfusion delay, donor reliability, and hemovigilance-related outcomes compared with conventional donor pathways. Methods This prospective analytical study included 400 transfusion episodes across tertiary hospitals in Bangladesh. Donor sources were categorized as social media (SM) or conventional (CON). The primary outcome was delay-to-transfusion. Secondary outcomes included donor-related irregularities, documentation completeness, near-miss events, and acute transfusion reactions. Multivariable logistic regression identified predictors of delay ≥4 hours. Results Social media–sourced donors were associated with significantly longer transfusion delays (5.98 vs 2.97 hours; p<0.001). Delay ≥4 hours occurred in 83.6% of SM cases versus 17.6% of CON cases (OR 23.78). Donor-related irregularities were observed in 85% of SM episodes and absent in CON donors. Safety outcomes did not differ significantly between groups. Social media donor sourcing remained the strongest independent predictor of delay (adjusted OR 18.09). Conclusion Unregulated social media–based donor recruitment introduces substantial delays and undermines system reliability without improving access. Integration of digital tools into regulated donor systems is essential to strengthen transfusion timeliness and hemovigilance in resource-limited settings.
Abstract Dengue is a viral infection transmitted by mosquitoes of the Aedes genus, responsible for millions of cases each year. In Benin, several outbreaks have been reported, particularly in Abomey-Calavi, where the first dengue-related death was recorded in 2019. In the absence of a specific treatment, vector control remains the primary preventive measure. However, the emergence of insecticide resistance, notably through kdr mutations, could compromise its effectiveness. This study aims to identify the dengue virus serotypes circulating in the commune of Abomey-Calavi and to assess the frequency of kdr mutations V410L , V1016G , V1016I , F1534C , and S989P in Aedes aegypti and Aedes albopictus mosquitoes. Mosquitoes were collected in August 2024 from four arrondissement Togba, Calavi, Akassato, and Godomey through morning and afternoon spraying. After morphological identification, molecular analyses were conducted to detect the viral serotypes and kdr mutations. A total of 218 Aedes mosquitoes were collected, with a predominance of Aedes aegypti (68.8%) compared to Aedes albopictus (31.2%). The overall dengue positivity rate was 23.39% (95% CI: 17.9–29.6). The most frequent serotype was DENV-3 (12.4%), followed by DENV-2 (6.9%), DENV-1 (5%), and DENV-4 (0.09%). Six mosquitoes were co-infected with two serotypes. Kdr mutations were detected in both species, with frequencies of 45% for V1016G , 44% for S989P , 40% for F1534C , 22% for V410L , and 19% for V1016I. Four mosquitoes carried three simultaneous mutations, while twenty-two carried two. These results highlight the active circulation of the dengue virus and the presence of kdr mutations in Aedes mosquitoes in Abomey-Calavi. However, no significant association was observed between dengue infection and kdr mutations, and their distribution was independent of the viral serotype. These findings emphasize the need for regular monitoring of kdr mutations to adapt vector control strategies and limit the spread of dengue.
Brucellosis is listed as a priority disease in low-income countries like Guinea, facing challenges in logistics, equipment, competence, and cost limitations for diagnosis. Serological diagnosis is mainly performed by the Rose Bengal agglutination test (RBT) in the veterinary sector. We have compared its discriminative capacity with more sophisticated and expensive serological tests, such as multi-species or species-specific ELISA kits and Complement Fixation test (CFT). A panel of 554 serum samples of pigs, goats, sheep, and cattle collected throughout Guinea from 2017 to 2019 where tested by RTB and ELISA tests in parallel at the Institut Pasteur de Guinée (Conakry) and the Brucellosis WOAH/EU Reference Laboratory of the French Agency for Food, Environmental and Occupational Health Safety (Maisons-Alfort, France). ELISAs performed equally across laboratories (Kappa =0.867–0.958); RBT and ELISA showed 94–95
Background Retaining repeat blood donors is essential for maintaining a safe and sustainable blood supply. While pain during venepuncture has been linked to lower donor return, the influence of donor-perceived needle quality on future donation remains unclear, particularly in low- and middle-income countries. This study examined whether perceived needle quality was associated with willingness to donate again among blood donors in Bangladesh. Methods We conducted a cross-sectional analytical study among 100 consecutively recruited whole-blood donors from participating blood donation centres in Bangladesh. Participants completed a structured questionnaire assessing demographic characteristics, donation history, procedural pain using a 10-point Visual Analogue Scale (VAS), pre-donation fear, perceived needle quality, vasovagal symptoms, overall satisfaction, and willingness to donate again on a five-point ordinal scale. Univariable ordinal logistic regression was used for variable screening, followed by multivariable proportional-odds ordinal logistic regression with purposeful variable selection. Statistical significance was set at p < 0.05. Results The mean donor age was 35.8 ± 11.8 years, and 88% of participants were male. High procedural pain (VAS ≥4) was reported by 73% of donors, whereas 48% expressed high willingness to donate again. After adjustment, procedural pain was the only independent predictor of future donation intention. Each one-point increase in pain score reduced the odds of greater willingness to donate again by 54% (adjusted OR 0.46, 95% CI 0.37–0.57; p < 0.001). Donor-perceived needle quality was not associated with willingness to donate again (adjusted OR 1.09, 95% CI 0.47–2.56; p = 0.840) and showed no association with procedural pain. Conclusions Procedural pain, rather than donor-perceived needle quality, was the principal determinant of willingness to donate again. Interventions that improve donor comfort and minimise venepuncture pain may strengthen donor retention. Larger prospective studies using actual donor return behaviour are warranted. ### Competing Interest Statement The authors have declared no competing interest. ### Author Declarations I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. Yes The details of the IRB/oversight body that provided approval or exemption for the research described are given below: Ethical review board of Comilla Medical College. Ref no- CoMC/ERB/02 I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals. Yes I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance). Yes I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable. Yes All data produced in the present study are available upon reasonable request to the authors
