The University Pitt Club, popularly referred to as the Pitt Club, the UPC, or merely as Club, is a private members' club of the University of Cambridge, with a previously male-only membership but now open to both men and women.
Multisystemic inflammatory syndrome (MIS-C) is an inflammatory condition temporally associated with COVID-19 in children; nevertheless, the clinical and immunologic spectrum of MIS-C is heterogeneous, and its long-term effects are unknown. During the period of August 2020 to December 2021, a total of 52 MIS-C cases were confirmed in pediatric patients from the Hospital del Niño DIF Hidalgo, diagnosed using criteria from the World Health Organization. All patients had serologic IgG confirmation of SARS-CoV2, the mean age of the patients was 7 years, and 94% of the patients did not have a previous underlying disease. In addition to the presentation of lymphopenia, neutropenia, and thrombocytopenia, elevations in D-dimer and ferritin levels were observed in all patients. There was clinical improvement with intravenous gamma globulin and corticosteroid treatment.
Objective: To test whether lesions related to epilepsy map to a common brain network. Background: Focal epilepsy is commonly caused by brain lesions, but it remains unclear why some lesion locations result in epilepsy while others do not. One possibility is that some brain regions or networks are more vulnerable than others. Identifying the brain regions or networks related to epilepsy could inform prognosis and guide interventions. Design/Methods: Lesion locations from patients with ischemic stroke-related epilepsy (n = 76) and control lesions (n = 625) were mapped to a common brain atlas. Traditional lesion mapping methods were used to test for associations between epilepsy and lesion location. Next, we computed the brain network functionally connected to each lesion location using human brain connectome data (n = 1000). Network connections associated with epilepsy were identified. Generalizability to other lesion types was assessed using independent datasets of four different lesion etiologies (n = 772) and a leave-one-dataset-out analysis. These connections were then used to generate a brain network map that best encompasses lesion locations related to epilepsy. Finally, we tested whether thalamic deep brain stimulation sites that improve seizure control were connected to this same network (n = 30). Results: We found that lesions associated with epilepsy occurred in multiple heterogenous locations spanning different lobes and vascular territories. However, these heterogenous lesion locations were part of a common brain network defined by functional connectivity to the basal ganglia and cerebellum (Vmax = 6.8, P < 0.001). Functional connectivity to these regions was associated with the risk of epilepsy across different lesion types (χ2 = 205.3, P < 0.001) and with therapeutic response to thalamic deep brain stimulation (r = 0.63, P < 0.005). Conclusions: Lesion locations related to epilepsy map to a common brain network defined by functional connectivity to the basal ganglia and cerebellum. Disclosure: Dr. Schaper has nothing to disclose. Dr. Nordberg has nothing to disclose. The institution of Dr. Cohen has received research support from NIH. The institution of Dr. Cohen has received research support from Simons Foundation. Mr. Lin has nothing to disclose. The institution of Mr. Hsu has received research support from Walter L. Copeland Fund of the Pittsburgh Foundation. Dr. Horn has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Boston Scientific. Dr. Ferguson has nothing to disclose. Dr. Siddiqi has received personal compensation in the range of $50,000-$99,999 for serving as a Consultant for Magnus Medical. Dr. Siddiqi has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Brainsway Ltd. Dr. Siddiqi has stock in Brainsway Ltd. Dr. Siddiqi has stock in Magnus Medical. Dr. Siddiqi has received intellectual property interests from a discovery or technology relating to health care. Mr. Drew has nothing to disclose. Louis Soussand has nothing to disclose. Dr. Winkler has received research support from National Institutes of Health. Dr. Simó has nothing to disclose. Jordi Bruna has nothing to disclose. Prof. RHEIMS has nothing to disclose. Prof. GUENOT has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Dixi Medical. Dr. Bucci has nothing to disclose. Dr. Nummenmaa has nothing to disclose. Dr. Staals has nothing to disclose. Dr. Colon has received personal compensation in the range of $500-$4,999 for serving as a Consultant for DEKRA. Dr. Colon has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for Livanova. Dr. Colon has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for Medtronic. Dr. Colon has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for De Baar. Dr. Colon has received personal compensation in the range of $0-$499 for serving as an Expert Witness for Rechtbank Zwolle. The institution of Dr. Colon has received research support from Zon/MW ZiN. Miss Ackermans has nothing to disclose. Dr. Bubrick has received personal compensation in the range of $500-$4,999 for serving as a Consultant for UniQure. Dr. Peters has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Neurelis. Dr. Peters has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for Neurelis. Dr. Peters has received personal compensation in the range of $10,000-$49,999 for serving on a Speakers Bureau for Neurelis. Dr. Wu has received stock or an ownership interest from Microsoft. Dr. Wu has received stock or an ownership interest from Disney. Dr. Wu has received stock or an ownership interest from Con Edison. Dr. Wu has received stock or an ownership interest from Cisco. The institution of Dr. Wu has received research support from NIH. The institution of Dr. Wu has received research support from AHA. The institution of Dr. Wu has received research support from Same You. The institution of Dr. Wu has received research support from Football Players Health Study. Dr. Wu has received intellectual property interests from a discovery or technology relating to health care. Dr. Wu has received intellectual property interests from a discovery or technology relating to health care. Dr. Wu has received intellectual property interests from a discovery or technology relating to health care. Dr. Wu has received personal compensation in the range of $500-$4,999 for serving as a grant reviewer with NIH. Dr. Rost has received personal compensation in the range of $5,000-$9,999 for serving as an Editor, Associate Editor, or Editorial Advisory Board Member for Stroke - AHA/ASA Journal. The institution of Dr. Rost has received research support from NIH. Dr. Rost has received publishing royalties from a publication relating to health care. Dr. Grafman has received personal compensation in the range of $500-$4,999 for serving as an Editor, Associate Editor, or Editorial Advisory Board Member for Elsevier. The institution of Dr. Grafman has received research support from NIH. The institution of Dr. Grafman has received research support from Templeton Foundation. Dr. Blumenfeld has nothing to disclose. Yasin Temel has nothing to disclose. Rob Rouhl has nothing to disclose. The institution of Juho Joutsa has received research support from Finnish Medical Foundation. The institution of Juho Joutsa has received research support from Sigrid Juselius Foundation. The institution of Juho Joutsa has received research support from Instrumentarium Research Foundation. The institution of Juho Joutsa has received research support from Turku University Hospital. Dr. Fox has received personal compensation in the range of $500-$4,999 for serving as an Editor, Associate Editor, or Editorial Advisory Board Member for Wiley.
Weighted Ensemble (WE) sampling is a rigorous multi-replica method for full-atom simulations of equilibrium and not-equilibrium processes [1,2]. The strategy this method uses can be summarized in three steps: i) initiation of independent simulations from a starting state of the system; ii) progress coordinate definition and replication of the trajectories advancing along it; iii) splitting/replicating or merging/culling simulations in undersampled or oversampled bins, in which the conformational space has been divided. WE method has been demonstrated to accurately reproduce the results obtained by using full-atom molecular dynamics simulations, herein called brute-force dynamics [3]. The ionic current through a membrane ion channel model was calculated by means of WE simulations. The results show that the I-V relationship accurately reproduce that obtained by using brute-force dynamics. Moreover, in presence of low ionic current, the efficiency of the WE approach increases greatly. Here, we aim to calculate the I-V relationship of the K+ TRAAK ion channel embedded in a POPC bilayer by applying the WE method. Research supported by NIH grants R21 NS091941-01. [1] G. A. Huber and S. Kim, Biophys J, 70, 97 (1996). [2] B. W. Zhang et al., J Chem Phys, 132, 052107 (2010). [3] J. L. Adelman and M. Grabe, JCTC, 11(4), 1907 (2015)
