胃食管反流病（gastroesophageal reflux disease,GERD）的发病率在世界范围内呈逐年上升趋势。目前我国对 GERD 的基本概念认识不足,并就相关问题没有达成一致意见,这种情况影响着我国在GERD 方面的诊疗和研究进展,有关 GERD 研究和诊断治疗的总体水平不高,因此有必要就 GERD 相关问题形成统一的认识。故2006年8月26日在三
Normal liver cells have a finite life span for which confined their application in biomedical research .Immortalization strategies based on recent advances in molecular biology have led to the creation of genetically stable, nontumorigenic immortalized cell line. Immortalized human hepatocyte cell lines with differentiated liver function would find broad application in pharmacologyand toxicology, artificial liver support, and liver constitution engineering. The article reviews These new immortalized hepatocyte cell lines, their application and future prospects are also discussed.
Fulminant hepatitis accompanied with hemolytic anemia represents the most serious and dramatic variant of wilson. The article reviewes the pathogenesis, clinical manifestation, laboratory test,diagnosis and therapy of FWD.
After the achievement of human genome project (HGP), the post-genome era is coming. The proteomics has become a hot. We have made a lot of research on cancer proteomics which is the new embranchment of proteomics. Cancer proteomics can observe the changes of kinds and quantity of protein which appear during the advance of carcinoma. Colorectal cancer is a very common disease. Cancer proteomics can be used to find the carcinoma biomarkers, the molecular mechanism and the anticancer drug targets.
Diabetics is prone to associated with various infections.But reports on relations of diabetics and Hp infection are scarce and not yet draw medical workers attention.Reports update has indicated that diabetics with Hp infection usually has more severe clinical symptoms,blood glucose is not easy to control,higher incidence rate of heart and cerebrovascular compli- cation are high,therapeutic efficacy is not content.
hepatocyte transplantation is effective in the treatment of serve hepatic disease.Porcine hepatocytes transplanta- tion can improve prognosis of acute hepatic failure rats.But some problems should be conquered in xenogeneic hepatocytes transplantation.
With the increase of development and application of new drugs and combined application of many varieties of drugs,the incidence rate of drug-induced liver injury has increased gradually,and this has caused our attention and study.Now it has made great advancement in genetics,proteomics etc.We will review the drugs,pathogenesis,clinical classification and ap- pearance,susceptible factors induced liver injury in this article.
Peptide YY(PYY) is a gut hormone that has inhibitory action on many tissues.It has been found that PYY ex- ists in many carcinoma tissues,and has inhibitory effect on pancreatic cancer,colon cancer,Barrett esophageal adenocarcinoma, breast cancer,prostate cancer and dyscrasia resulted from cancer.
目的:研究络沙坦(Losartan)对急性坏死性胰腺炎(ANP)大鼠肺损伤的保护作用.方法:SD大鼠30只随机分为3组:正常对照组、ANP组、Losartan治疗组.采用大剂量精氨酸腹腔注射诱导ANP模型,治疗组于造模后30 min给予Losartan200 μg/kg,2次,每次间隔1 h.各组于造模后24 h处死大鼠,观察大鼠血浆血管紧张素Ⅱ(AngⅡ)、肾素(PRA)、淀粉酶(AMY)、TNF-α、IL-1β、肺组织髓过氧化物酶(MPO)、肺湿/干重比值、胰腺/体重比值、胰腺和肺组织病理变化.结果:ANP组血浆AngⅡ、PRA、AMY、TNF-α、IL-1β、MPO、肺湿/干重比值、胰腺/体重比值较对照组明显升高;Losartan治疗组血浆AngⅡ、PRA较ANP组变化不明显,AMY、TNF-α、IL-1β、MPO、肺湿/干重比值、胰腺/体重比值较ANP组明显下降,胰腺和肺组织病理损伤明显减轻.结论:络沙坦对血浆AngⅡ、PRA无明显影响,可以减少TNF-α、IL-1β、MPO的产生,对ANP肺损伤具有保护作用.
Irritable bowel syndrome(IBS) used to be thought as a chronic functional gastrointestinal disease. This article reviews the change of proinflammatory cytokines and antiinflammatory cytokines in post-infection IBS cases and presents that the imbalance between both cytokines facilitating the onset of IBS.
5-羟色胺及其受体广泛分布于胃肠道。5-羟色胺7受体有多种生理功能,对胃肠运动和感觉的调控有重要作用。对5-羟色胺7受体的分子结构、组织分布、生理功能已进行了相关研究,并日益受到重视。深入开展5-羟色胺7受体在消化道的生理功能和功能性胃肠病中的病理机制的研究,有助于进一步阐明功能性胃肠病的发病机制及开发新的有效治疗药物。
Objective: To study the effect of cell apoptosis on rat pancreatic acinar cells injury in dietary hypertriglyceridemia. Methods: Male SD rats were assigned to one of two experimental groups and fed for 4 weeks with high fat diet or normal diet. Blood samples were taken to measure triglyceride and cholesterol in plasma. Pancreatic acinar cells were isolated by collagenase digestion. Cell viability was determined by typan blue dyeing at different time points. Cells treated with CCK-8 were examined under transmission electron microscope. Expressions of bax and bcl-2 were investigated by RT-PCR. Results: Compared with the normal diet group, the level of triglyceride was increased significantly in high fat diet group(P0.05). Cell mortality obviously increased after 6 h of isolation in high diet group (P0.05). Enzyme vacuoles were observed in cells of normal fat diet group treated with 10pmol/L CCK-8. Cell apoptosis, necrosis and decreased density of intracellular zymogen granules were observed in both groups with 10nmol/L CCK-8 and in high fat diet group with 10pmol/L CCK-8. The ratio of bax to bcl-2 mRNA was significantly higher in high fat diet group(P0.05). Conclusions: The present results indicate high fat diet can induce hypertriglyceridemia in rat and increased triglyceride can injure the pancreatic acinar cells. Pancreatic acinar cells apoptosis and elevated ratio of bax to bcl-2 may play an important role.
Somatostatin is a kind of cytokine having general biologic activity.Recent studies have shown that somatostatin and somatostatin anolog can inhibit the tumor cell proliferation and induce the tumor cell into apoptosis.Not only can it inhibit proliferation of neuroendocrine tumor,but it has inhibitory action on ordinary solid tumor.Somatostatin and somatostatin anolog in the therapy of hepatocellular carcinoma have been used in clinic.Now this article mainly reviews recent research progress of somatostatin and somatostatin anolog in the therapy of hepatocellular carcinoma,in order to provide new channel and way for the treatment of hepatocellular carcinoma.
With the changment of life style and dietary structure, hyperlipidemia gradually becomes the third cause of sever acute pancreatitis. At present, literatures about hyperlipidemic sever acute pancreatitis (HL-SAP) are almost introductions of single treatment or case reports. This article gives an overview of the research progress in HL-SAP.
The response to injury is one of wound healing and subsequently fibrosis.Injury and wounding lead to cirrhosis ultimately in many patients (although not all patients).Recent literature indicates that the hepatic stellate cell is the central component in the fibrogenic process.Stellate cells undergo a transformation during injury that has been termed activation.Ac- tivation is complex and multifaceted,but one of its most prominent features is the synthesis of large amounts of extracellular matrix.It is noteworthy that even advanced fibrosis (or cirrhosis) is reversible.This article emphasizes mechanisms underlying fibrogenesis,and reviews current antifibrotic therapies as well as potential future approaches.
Fatty liver disease is one of the main causes of cirrhosis. We have known that fatty liver disease can progress to liver cirrhosis and then to hepatic carcinoma. Nowadays more and more research have confirmed that fatty liver disease can progress to primary liver cancer independent of cirrhosis. But the pathogenesis between fatty liver disease and primary liver cancer is unkown. This article is mainly about the possible pathogenesis how fatty liver disease progress to liver cancer.
Helicobacter pylori(Hp) found by Warren and Marshall in 1983 is a Gram-negative bacterium identified as the aetiological agent of peptic ulcer disease, gastric adenocarcinoma and B-cell mucosal-associated lymphoid tissue (MALT) lymphoma. Oxygen radicals induced by Hp can lead to injury of gastric mucosa epithelia and formation of ulcer. In addition, oxygen radicals result in imbalance of proliferation and apoptosis of gastric mucosa epithelia, damage of biomacromolecule such as lipid, protein and DNA and therefore gastric cancer.
Endoscopic mucosal resection has been shown to be effective in treating mucosal malignancies.However,en bloc resection is often not achieved using conventional methods.New techniques developed in Japan, including the application of different types of knife, such as the insulated-tip diathermic knife(IT-knife).This article focused on the clinical application of this new knife in EMR.
Hepatocyte growth factor is a cytokine with multifunction,which plays an important role in promoting hepatocyte proliferation,inhibiting activation of hepatic stellate cell (HSC) and so on.During the process of liver fibrosis,hepatocyte is continuly injured,which promotes hepatocyte regeneration,activation of HSC and deposition of extracellular matrix (ECM) in the hepatic sinusoid.All these enhance the progression of liver fibrosis.This review summarizes the biological function of hepa- tocyte growth factor in liver fibrosis.
Severe acute pancreatitis(SAP) is a kind of disease which the beginning is acute, the progress is quick and complicated therapy. Cure mode has changed continuously with extremely study the pathogenesis and pathologic physiology. The key of medicine treatment is repressing pancreatin to secrete, rectifying internal environment mess, and easing the damage of the pancreas outside organ . Therapy have been extensive affirmed that of somatostatin repressing pancreatin to secrete, antibiotic anti-inflammatory, essential nutritional support and curing acute bile pancreatitis by endoscope, currently.In this article we review the advancement in the medicine treatment.
