Objective To investigate the entrapment efficiency and the release rate of Aspirin encapsulated into noiosomes,and provide the guidance for the study of the new drug delivery system.Methods Niosomes were prepared with nonion surfactant span-60 and cholesterol by ultrasonication.And the entrapment efficiency of Aspirin encapsulated into noiosomes and release profiles in simulated gastric and intestinal fluid were studied.Results The results showed that the entrapment efficiency of aspirin by noiosomes reached 91% under the condition that the molar ratio of the span-60:cholesterol equaled to 1﹕1 and the concentration of aspirin was 0.2mg/mL.The results also indicated that in simulated gastric and intestinal fluid,the release rate of Aspirin entrapped in niosomes was 50% after 3h,however the release rate of free Aspirin was 98% under the same condition.Conclusion The entrapment efficiency of Aspirin entrapped by niosomes was high enough and the release rate of the entrapped drug was slow obviously.
目的调查与评价洸府河（市区段）铬污染情况。方法选择在济宁市区凯赛大桥等八处水域采集水样,采用二苯碳酰二肼分光光度法测定水中铬（Ⅵ）及总铬的含量。结果整个洸府河（市区段）水中铬（VI）含量和总铬含量均低于《地表水环境质量标准基本项目标准限值（GB3838-2002）》Ⅱ类的标准限值,其中凯赛大桥采样点的铬（VI）含量低于Ⅰ类的标准限值,而且,各采样点水中总铬含量均高于铬（VI）含量,说明水中还有其他价态的重金属铬存在。结论得到洸府河的准确的铬污染参数。并以此为依据分析河水中的铬来源、评价其影响并积极探讨其治理方式、方法。
Objective To estimate the clinical effective of total knee arthroplasty(TKA) for osteoarthritis of knee joint.Methods Twenty-seven patients(30knees) with osteoarthritis of knee joint were selected for TKA(6 males,21 females).The patients aged from 50 to 76 years(average 63.2 years).Left knee was in 11cases and right knee was 13 cases.Simultaneous bilateral was performed in 3 cases.Results All patients were followed up from 4 to 24 months with the average of 10 months.According to HSS system,the results were excellent in 25 cases(83.3%) and good in 4 cases(13.3%) and fair in 1 case(3.3%),and the excellent and good rate was 96.7%.Conclusion Total knee arthroplasty can release knee pain and improve knee function.The short term effect is satisfactory,but long term follow-up is needed.
Objective To provide basis for the relevant departments of scientific planning rural safe drinking water project and developing preventive measures for water-borne diseases. Methods The 2005~2007 monitoring data of infectious diseases management system of Jining were analyzed,five or sixth grade students and parents of three central primary schools were randomly selected to conduct a questionnaire survey of water-borne diseases. Results The high-risk population of intestinal infectious diseases is mainly farmers and diaspora children in Jining.90.4% farmers have the habit of drinking boiled water,92.2% farmers know that wells should stay away from sources of pollution,87.3% farmers can clean water containers regularly,Respectively 54.6% and 35.6% of rural residents and students know the causes of water-borne communicable diseases,and aware of the diseases caused by iodine-deficient diet are 9.8%,4.5%,know that diseases caused by excessive fluoride of drinking water are 5.5%,46.7%.Farmers access to knowledge of water-related diseases prevention mainly through newspapers and television.96.5% farmers and 94.6% students want to learn more knowledge about water-borne disease prevention and control. Conclusion Rural residents have better health habits of drinking water,but the awareness rate of water-related disease prevention knowledge is relatively low,Relevant departments to carry out health education for rural residents and students of water-related disease prevention is insufficient.
Objective To investigate protection of ischemic precondition(IPC)and postcondition(IPO)on renal ischemia reperfusion(I/R)injury in rabbits.Methods Firstly renal I/R injury models were used in this experiment.Forty male rabbits were randomly divided into 4 groups(n=10 each):groupⅠ:sham operation,groupⅡ:I/R injury,groupⅢ:IPC,groupⅣ:IPO.After reperfusion 60min,blood MDA content and SOD activity were measured.The changes of renal tissue were detected by microscope.Results SOD activity was significantly lower whereas the serum MDA content was significantly higher in I/R group、IPC group and IPO group than in sham group(P0.05).The serum SOD activity was significantly higher whereas MDA content was significantly lower in IPC group and IPO group than in I/R group.Microscopic examination showed that the renal I/R injury was significantly attenuated in IPC group and IPO group.Conclusion IPC and IPO attenuate the renal injury induced by I/R in rabbits,and the protective effect is associated with the enhancement of the body antioxidation.
Objective To investigate the preparation method and the transdermal diffusion of aspirin inverse microemulsion. Methods The inverse Microemulsion was prepared with 14 isopropyl acid lipid as the oil phase,lecithin as the surfactant,isopropanol as the cosurfactant and the distilled water as water phase.The boundaries of the inverse microemulsion region were determined by using maximum water content method.And the transdermal permeation of the drug microemulsion was studied by using the RYJ-6A with the phosphate buffer solution(pH=7.4) as receptor fluid. Results The boundaries of the inverse microemulsion region could attained the maximum value with the K m=1∶2,the inverse microemulsion with aspirin,the accumulate permeation equation was Q=534.72t-519.4,transdermal diffusion constant was J SS =534.72 μ g/(h·cm 2),and the diffusion coefficient was K r=0.2674cm/h. Conclusion The aspirin microemulsion had the ability to enhance transdermal absorption.
Objective To establish the methods of isolation,culturing of neural stem cells(NSC)in vitro and observe the characteristics of NSC growth.Methods NSC were isolated and cultured by microdissection.mechanical blowing and serum-free suspension culture.The cells were identified by detecting the differential NSC antigen―nestin,the ability to proliferate continuously and differentiate into neurons and glial cells by inducement.Results The cultivated cells have the ability to proliferate continuously.They expressed nestin protein and can differentiate into neurons and glial cells by inducement.They have reproduced for above 20 generations.Conclusion The method can be used to isolate and culture NSC from embryonic rat brain.the cell identified as NSC possess the ability of proliferation and self-reproduction.The method of isolation and culture of rat NSC were successfully established.
Objective To study the effects of hand transmitted vibrations on sperm quality in coal mine drillers.Methods The count﹑motility ﹑percentage of motile and morphology of sperm in semen were measured and analyzed in 60 coal mine drillers.Through zona-free hamster oocyte test(ZHOT) and hypoosmotic swelling test(HOS)to valuate the oocyte penetrating rate and hypoosmotic swelling rate.Results Compared with Compared groups,the coal drillers expoured to vibration the motility ﹑percentage of motile 、 the oocyte penetrating and hypoosmotic swelling rate of sperm were much lower,the morphology rate were much higher,there were significant differences(P0.01).Conclusion The hand transmitted vibration of coal mine drillers resulted the motility ﹑percentage of motile 、 the oocyte penetrating rate and hypoosmotic swelling rate in semen significant lower,the morphology rate in semen were significant higher,thereby affect the sperm quality.
Objective To compare and evaluate the prognostic value of three prognostic systems namely acute physiology and chronic health evaluation Ⅲ(APACHE Ⅲ) simplified acute physiology score Ⅱ(SAPS Ⅱ),and acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ).on intensive patients in emergency department by means of statistical analysis.Methods Data of 211 patients were collected at admission.APACHEⅢ、SAPSⅡ score and APACHEⅡscore were calculated in 24 hours.To analyze mortality of patients in different score,to estimate dependability of them,The difference were compared between PHMs of APACHEⅢ,SAPSⅡ,APACHEⅡand actual mortality of all patients.The discriminations were compare among APACHEⅢ,SAPSⅡand APACHEⅡ.Results There have significantly difference between survivor group for scoring values and PHMs of all three evaluating systems(P<0.05).There have no significantly difference among APACHEⅢ(0.868),SAPSⅡ(0.851)and APACHEⅡ(0.857) for AUROCCs.Conclusion All three evaluating systems can be used to evaluate the severity of emergency intemal intensive patients.
Objective To investigate the correlated risk factors and the changes of related clinical parameters about diabetic peripheral neuropathy of type 2 diabetes(T2DM).Methods 280 patients with T2DM were randomly divided into group1(no DPN) and group 2,we detected their correlation of 22 clinical data,including course of the disease,blood pressure,blood glucose,glycosylated hemoglobin(HbAlc),total serum cholesterol,triglyceride,C-peptide etc.The resucts were analyzed by t test,χ2 test and Logistic regression.Results The β2-microglobulin(β2-MG,t=-2.403,P0.05),age(t=-2.617,P0.01) and the diabetic course(t=-5.653,P0.01) in group2 were significantly higher than that in group 1;There has the positive correlation between the β2-microglobulin(β2-MG OR=1.344,P0.05) or diabetic course(OR=1.135,P0.01) and diabetic peripheral neuropathy of T2DM.Conclusion The increase levels of β2-MG and diabetic duration were risk factors of diabetic peripheral neuropathy T2DM.
目的 了解本辖区食品、公共场所从业人员HBsAg 感染情况,为更好地制定防制对策提供依据.方法 采用间接血凝法检测辖区内16602名食品、公共场所从业人员血清中HBsAg,并对结果进行统计分析.结果 16602名食品、公共场所从业人员HBsAg 阳性检出率为1.5 %,其中,男、女性阳性率分别为2.04 %和1.15 %,男性高于女性(χ2= 21.02,P<0.005).16～25岁组阳性率最高(1.93 %),其它组相对较低,差异有显著性 (χ2= 19.19,P<0.005).HBsAg阳性滴度1:4096者居多,占81.93%.结论 本辖区食品公共场所从业人员HBsAg阳性率相对较低,但多数HBsAg阳性者的HBsAg滴度较高,具有较强的传染性.因此,应加强从业人员的HBsAg检测、卫生知识培训、健康教育和乙肝疫苗注射.
随着社会经济的发展和医学模式的转变、疾病谱的变化,特别是2003年的SARS、2004年的禽流感以及艾滋病的蔓延,全球人民所面临的公共卫生问题也在迅速演变.疾病防治形势的变化给预防医学带来了新的发展机遇.同时又给预防医学教育工作者提出了更高的要求[1].
1 临床资料 一般资料:选择2008年2月至2009年7月,来院做人流术的妇女268例.年龄19～40岁,平均年龄29.5岁,妊娠6周～11周,平均8.5周.术前均做妇科检查,排除性传播疾病、盆腔感染、宫颈炎和阴道炎等.
目的 探讨脊髓海绵状血管瘤的诊断和显微外科治疗.方法 系统回顾性分析14例脊髓海绵状血管瘤患者的临床特征、诊断和显微外科治疗方法,评价患者术前术后的脊髓功能. 结果 14例均采用显微外科治疗,术后病理证实为海绵状血管瘤.髓内型11例,髓外硬膜下型2例,硬膜外型1例.血管瘤全切除13例,次全切除1例.术后患者症状好转9例,无变化3例,加重2例.无手术死亡.结论 MRI对脊髓海绵状血管瘤诊断的特异性高,显微手术治疗效果好.
网络成瘾(Internet Addiction Disorder简称IAD)是近年网络心理学(Cyberpsychology)研究的热点,网络成瘾指在无成瘾物质作用下的上网行为冲动失控,表现为由于过度使用互联网而导致个体明显的社会、心理功能损害[1,2,3].本研究试图通过对大学生的网络使用情况及其可能的影响因素的调查,对当代大学生网络社会问题的主要表征、网络成瘾者个性特征、网络成瘾的影响因素等方面进行初步的探讨和分析.
目的 探讨鸦胆子油乳液对豚鼠耳部银屑病样病理改变治疗作用和安全性.方法 5%普萘洛尔软膏外用构建豚鼠耳部的银屑病样病理改变模型,设对照组及不同浓度鸦胆子油乳液治疗组.光镜下观察各组局部皮肤病理变化.结果 鸦胆子油乳液溶液治疗组Baker评分和炎症细胞浸润数出现不同程度下降,与赋形剂对照组及造模后观察组比较有统计学显著性差异(P＜0.05).5%鸦胆子油乳液溶液治疗组与2.5%鸦胆子油乳液溶液治疗组相比,Baker评分及炎症细胞浸润数均存在显著差异.鸦胆子油乳液DMSO溶液外涂未见局部刺激、过敏症状,实验豚鼠各主要脏器未发现组织病理学改变.结论 鸦胆子油乳液DMSO溶液外涂可改善豚鼠耳部银屑病样模型的病理变化,与浓度高低成正相关.
目前,我国的痴呆患者大多居于家中,由亲属给予直接照顾[1].在常年的照顾压力下,痴呆亲属照顾者的身心健康及生活质量受到很大影响,照顾者负担感持续加重,并对其痴呆亲属的生活质量及病情发展带来很多不利影响.因此,需要相关人员及时给予有效干预,以缓解居家痴呆患者照顾者的负担,促进患者及其照顾者的身心健康.
目的 探讨隐匿性巩膜裂伤病人的手术疗效与手术时间及方式的关系.方法 对我院2006年至2008年的36例(36眼)巩膜裂伤的病人进行回顾性分析.结果 患者在伤后立即就诊者26名(26眼),其中24名(24眼)患者术后视力有进步.有9名(9眼)患者在伤后7天内来诊,其中只有4名(4眼)患者视力有所恢复.而有1名(1眼)病人在受伤21天来诊,视力较差.36例患者中28例患者行玻璃体切割术,23例患者术后视力有改善,3例患者最后行眼摘.结论 隐匿性巩膜裂伤病人要及时就诊,及时手术探查发现伤口并清创缝合.术后及时行玻璃体切割手术是挽救视力,保存眼球的关健.
Objective To explore the relationship between the behavior of type A characteristics of coronary heart disease and combine six biochemical markers.Methods Test 89 patients who have been diagnosed with coronary heart disease by type A Behavior Questionnaire and assay uric acid,glucose,total cholesterol,triglyceride,high-density lipoprotein,low density lipoprotein six biochemical indicators by full-automatic biochemical analyzer.Results Among follwed 70 patients with coronary heart disease,44 cases of actors had A-type behavior,26 cases had non-A type behavior;CH and TABP score of patients with coronary heart disease were higher than NORTH control;TH,CH and TABP score of patients with coronary artery disease in different age groups are different;six biochemical findings in A and non-A cases had no significant difference.Conclusion In Patients with Coronary Heart Disease,A type of actors are more than that non-A type cases,but the low age group's score is higher than that the high age group.
目的 探讨胃血管球瘤的临床病理学特征和鉴别诊断.方法 对3例胃血管球瘤的临床特征、组织学形态和免疫表型进行观察.结果 3例肿瘤均发生在胃窦部,形态学改变与发生于软组织的肿瘤相似,均表现为良性,所有病变均能显示平滑肌分化.肿瘤细胞Vimentin、SMA均表达阳性,desmin、HMB-45、S-100P、CD34和CD117均阴性.结论 胃的血管球瘤显微镜下易与胃肠间质瘤和神经内分泌肿瘤混淆,因此应充分引起临床和病理医生的注意.
