Chondrosarcoma (CHS) is a common malignant bone sarcoma and its occurrence increases with age. microRNAs (miRNAs) are a class of noncoding RNAs that participate in various biological processes and disease pathogenesis by targeting functional messenger RNA (mRNA). However, the modulation of miRNAs in CHS remains largely unknown. In this study, we performed integrative analysis to explore the expression profiles of miRNAs and mRNAs, together with their interaction networks in human CHS tissues and cell lines by RNA-seq (miRNA and mRNA). A total of 133 and 796 differentially expressed miRNAs and mRNAs were identified (|Fold change| ≥ 2 and P-value ≤ 0.5). miRNA-mRNA regulatory interactions between 55 miRNAs and 242 mRNAs were screened by the Pearson correlation analysis and target prediction. mRNAs in the network were enriched to 145 Gene Ontology terms and 35 Kyoto Encyclopedia of Genes and Genomes pathways. Specifically, some key regulators (hub-miRNAs) in the network (miR-622, miR-4539, miR-145, miR-25, and miR-96) were suggested to play important regulatory roles in the pathogenesis of CHS. In addition, functional experiments validated that miR-622 regulated CHS cell proliferation by targeting bone morphogenetic protein 1 (BMP1).

Objective: The aim of this study was to investigate differences in the gait patterns of patients with ceramicon- ceramic large-diameter-femoral-head hip arthroplasty (LHA) and those with standard-diameter-femoral-head hip arthroplasty (SHA) within one year after surgery. Methods: Between October 2012 and March 2014, 30 patients who had undergone LHA (36 mm) and 30 patients who had undergone SHA (28 mm) were selected. One year after surgery, postoperative gait analysis of each patient was performed with the Tecnobody gait analysis system, and gait parameters (ipsilateral/contralateral ratios for each patient) were measured. Results: Ipsilateral/contralateral ratios of step cadence, step time, proportions of the gait cycle accounted for by the single-leg stance phase and the swinging phase and the Harris score of the hip joint did not differ significantly between the two groups. However, ipsilateral/contralateral ratios of step length, gait velocity, hip flexion/extension, adduction/abduction, rotation, and knee flexion/extension were significantly higher in the LHA group than in the SHA group (P < 0.05). Conclusion: One year after surgery, gait parameters and joint range of motion were superior in patients who underwent LHA compared to those who underwent SHA. A large-diameter-femoral-head prosthesis may be more effective in the recovery of joint function in patients after surgery.

BACKGROUND:Lateral retinacular release is effective to treat anterior knee pain, but it has not been confirmed through the parameters of the knee joint. OBJECTIVE: To analyze the relevant parameters on the axial X-ray films of the patela before and after lateral retinacular release and then to find out the efficient parameters for patelofemoral joint disorders on the axial X-ray film of the patela, which are of most clinical significance. METHODS: Fifty patients with anterior knee pain were included randomly who admitted at the Department of Joint Surgery, Affiliated Hospital of Jining Medical University from February 2009 to December 2013, including 45 cases of Outerbridge I-III undergoing arthroscopic lateral retinacular release+articular cartilage repair and 5 cases of Outerbridge IV undergoing lateral retinacular release+knee joint surface replacement. RESULTS AND CONCLUSION:After lateral retinacular release, the congruence angle, patelofemoral index and tilt angle were al reduced significantly (P < 0.05 orP < 0.01), but there was no change in the lateral patelofemoral angle (P > 0.05). The patelar trajectory restored to the corresponding central position of the femoral condyle, and meanwhile, no joint hematoma, deep vein thrombosis and patelar subluxation occurred. These findings indicate that the lateral retinacular release is of great significance for treatment of anterior knee pain induced by lateral patelofemoral compression.

[目的]探讨关节腔内应用抗纤溶药物氨甲环酸对全膝关节置换术后失血量的影响.[方法] 2012年6月～2012年12月,选取100例行初次单侧全膝关节置换术患者,男28例,女72例;年龄55～78岁,平均65.5岁;骨性关节炎76例,类风湿性关节炎24例,病程1 ～15年,平均6.5年.随机分为A、B两组,每组50例.A组在缝合关节囊后向关节腔内注射氨甲环酸1g,溶于50 ml生理盐水;B组仅给予等量生理盐水关节腔内注射.记录术后失血量、输血量、输血例数、术后血红蛋白浓度和术后3h纤维蛋白原、凝血酶原时间、活化部分凝血活酶时间等为评价指标.观察患者是否出现下肢深静脉栓塞的临床症状,并于术后第7、14 d行下肢血管多普勒超声检查.[结果]A、B组术后可见失血量、输血量和输血例数比较差异有统计学意义(P＜0.05);术后血红蛋白浓度,A组明显高于B组(P＜0.05);两组患者术后3h纤维蛋白原、凝血酶原时间和活化部分凝血活酶时间的比较差异无统计学意义(P＞0.05).术后未发现下肢深静脉血栓形成.[结论]在全膝关节置换术中关节腔内应用氨甲环酸能明显减少患者术后失血量,降低输血率和输血量,并且避免了静脉内应用可能带来的并发症.

BACKGROUND: The abnormality of some parameters such as congruence angle has statistical significance on anterior knee pain has been confirmed under CT measurement, but has not been confirmed through clinical operations. OBJECTIVE: To analyzes the relationship between the parameter of knee joint and anterior knee pain through measuring the parameters such as congruence angle, patel ofemoral index, lateral patel ofemoral angle and tilt angle. METHODS: Fifty patients with anterior knee pain caused by varying degrees of atel ofemoral cartilage damage were included, and the parameters such as congruence angle, patel ofemoral index, lateral patel ofemoral angle and tilt angle were measured on the patel ar X-ray axial film. The parameters were compared with those of the other 50 patients without anterior knee pain and only with meniscus injury. RESULTS AND CONCLUSION: There were significant differences in congruence angle, patel ofemoral index and tilt angle on the patel ar X-ray axial film, and there was no significant difference in lateral patel ofemoral angle. The congruence angle and patel ofemoral index have important diagnosis value for the patients with anterior knee pain caused by patel ofemoral joint disorders and are considered as the most effective parameters which has clinical efficacy for the patients with patel ofemoral joint disorders.

目的 探讨自体骨植骨应用于全髋关节置换术(THR)中髋臼缺损的临床疗效.方法 对50例(50髋)髋臼缺损的全髋关节置换术进行分析,根据骨缺损情况进行髋臼自体骨植骨(结构性植骨、颗粒植骨).结果 50例获平均随访24.2个月,49例髋臼植骨处愈合.术后2周Harris评分67～82分,平均79.5分;术后1年86.5～94.6分,平均91.3分.结论 髋臼自体骨植于髋臼骨缺损处,可以恢复髋臼的完整性,增加髋臼的骨覆盖,提高THR术后髋臼假体的稳定性.

Objective To explore.the effect of operative treatment for more than 70-year-old patients with osteoporotic hip fracture,and analyze the clinical feature,perioperative management and choices of operative treatment.Methods One hundred and fifty-nine patients of more than 70-year-old with osteoporotic hip fracture were operated from June 2007 to October 2010.Fracture type:38 cases with intertrochanteric fracture while femoral neck fracture in 121 cases.Results One hundred and thirty-five cases were followed up after operation ranging from 3 to 43 months.There were 2 cases by proximal femur nail internal fixation,13 cases by proximal femur locked plate internal fixation,15 cases by dynamic hip screwinternal fixation,48 cases by bipolar femoral head arthroplasty and 57 cases by total hip arthroplasty.The Harris scores of various surgical method above respectively were 100.0％ (2/2),76.9％ ( 10/13 ),80.0％(12/15),89.6％ (43/48),96.5％ (55/57).Conclusions The operation plays an important role in the treatment of elderly osteoporotic hip fracture,having so many advantages as earlier mobilization,less complications and better life quality.But it is important to pay attention to perioperative management and choose reasonable surgical approach according to classification of fracture.

目的 探讨人工髋关节翻修重建术的翻修原因和临床疗效.方法 对2005年6月至2009年6月行人工髋关节翻修重建术28例患者进行随访,用Harris评分法,对人工髋关节翻修重建术的临床疗效进行评估.结果 28例患者术后随访6～48(34.0±8.6)个月.27例患者疼痛消失,关节功能恢复良好,1例患者因翻修术后发生感染,给予清创灌洗及抗生素抗感染治疗后得到控制.术前Harris评分(45.0±14.9)分,术后(89.0±10.2)分,术前与术后Harris评分比较差异有统计学意义(t=4.58,P=0.0042).结论 疼痛性无菌性假体松动是人工髋关节置换术主要的翻修原因,术中应根据患者年龄、骨缺损的类型等选择不同的假体类型,规范手术操作,加强功能锻炼,提高临床疗效.

目的:评价全膝关节表面置换术治疗膝关节骨关节炎的临床疗效.方法:本组27例30膝膝关节骨关节炎患者行人工全膝关节表面置换术,一期单膝置换24例,双膝同时置换3例,均采用后方稳定性假体.结果:本组随访3月～33月,平均24月.应用HSS评分系统进行分析,优23膝,良4膝,可3膝,差0膝.优良率90%.结论:人工全膝关节表面置换术是治疗晚期膝关节骨关节炎的有效方法.

Objective To investigate the clinical effect of bilateral simultaneity TKA inthe treatment of bilateral knee joint diseases. Methods 23 patients were treated by bilateral simultaneity TKA between March 2005 and January 2009,which consist of 18 cases of osteoarthritis and 5 cases of rheumatoid arthritis. 23 patients were followed up for 12 to 56 months,average of 38 months,to evaluate the clinical effect according to HSS score system. Results The average scores were 31 preoperatively and 89. 4 postoperatively,the average rang of motion was 76°preoperatively and 105°postoperatively,the operation effects were excellent in 34 knees ( 73. 9%) ,good in 9 knees( 19. 6%) ,normal in 3 knees( 6. 5%) and poor in 0 knee,the excellent good rate was 93. 5%. The patients' pain,joint function,activity,abnormality and stability were improved transparently after operation. All patients had no complications of infection. Conclusion The bilateral simultaneity TKA is a effective method in treating bilateral knee joint diseases,which has prominent clinical effect,the rate of compli-cations is no evidently increasing,too.

目的 对比分析人工全膝关节置换术(TKA)中髌骨成形与髌骨置换的短期临床疗效.方法 回顾性分析2006年1月至2006年12月因骨关节炎行TKA的33例(36膝)患者,其中髌骨成形26膝,髌骨置换10膝.所有患者术后随访30～41个月,平均35.3个月.结果 通过HSS评分及髌前疼痛等方面对比分析髌骨成形组和髌骨置换组疗效,结果均无显著性差异(P>0.05).结论 从近期临床疗效分析,人工全膝关节置换术中进行髌骨成形和髌骨置换效果无明显差异.

我们选择2007年7月至2009年7月的172例208髋人工髋关节置换术(Total hip arthroplasty,THA)患者,用Harris评分方法对患者手术前后的功能进行了评估,报道如下.

目的 观察人工全髋关节置换术治疗强直性脊柱炎合并髋关节强直的临床疗效.方法 对强直性脊柱炎引起的22例髋关节强直(26个髋关节)患者行人工全髋关节置换术治疗,回顾性分析其临床疗效.结果 22例术后平均随访26个月,髋关节活动度明显改善,步态基本正常,无严重并发症发生.结论 人工髋关节置换术可明显增加髋关节活动度,改善髋关节功能,提高生活质量,是治疗强直性脊柱炎并髋关节强直的有效方法.

Objective To estimate the clinical effective of total knee arthroplasty(TKA) for osteoarthritis of knee joint.Methods Twenty-seven patients(30knees) with osteoarthritis of knee joint were selected for TKA(6 males,21 females).The patients aged from 50 to 76 years(average 63.2 years).Left knee was in 11cases and right knee was 13 cases.Simultaneous bilateral was performed in 3 cases.Results All patients were followed up from 4 to 24 months with the average of 10 months.According to HSS system,the results were excellent in 25 cases(83.3%) and good in 4 cases(13.3%) and fair in 1 case(3.3%),and the excellent and good rate was 96.7%.Conclusion Total knee arthroplasty can release knee pain and improve knee function.The short term effect is satisfactory,but long term follow-up is needed.