Objective To utilizie bibliometric and visual analyses to study mesenchymal stem cells(MSC)for diabetes mellitus(DM)process and content of research.Methods Literature published in the Web of Science core database from January 2005 to March 2024 was visualised and analysed using software such as VOS-viewer,CiteSpace and R language.Results A total of 2 940 core documents were included.Germany,the United States and Italy are the most influential countries in this field,Harvard University is the central insti-tution collaborating in this field,Stem cell research&therapy is the most relevant journal to this field,and Diabetes is the most influential journal in this field.Current research in this field focuses on four directions:oxidative stress,pancreatic β-cell dysfunction,DM complications,and DM invasive therapy.Current research hotspots are in the areas of exosomes and invasive therapy.Conclusion Mesenchymal stem cell are still the frontier field of DM treatment,and the research mainly focuses on the treatment mechanism and the treatment of complications,and the future hot spots will focus on exosome and wound therapy.
Cochrane Library、Web of Science、PubMed、 Embase. Main outcome(s)(1) Endotoxin (EU/ml), (2) Liver function: alanine aminotransferase (ALT) in U/L, aspartate aminotransferase (AST) in U/L, total bilirubin (TBIL) in μmol/ml, albumin (ALB) in g/L, intestinal flora in IgCFU/g. Quality assessment / Risk of bias analysisCochrane bias risk assessment tool.Strategy of data synthesis Software RevMan5.3 was used solely for data analysis.The selected outcome indicators were all continuous variables, and the measurement methods and units of each indicator were the same.Weighted Mean Difference (WMD) and 95% Confidence Interval (CI) were used as effect sizes for data analysis.I 2 was used to test test the size of the evaluation heterogeneity.If P > 0.1 and I 2≤50%, the fixed effects model was chosen; if P≤0.1 and I 2＞50%, the random effects model was selected.The difference was statistically significant at P<0.05. Subgroup analysisSubgroup analysis was performed according to the administration of doctor's bacteria ≤15 days, > 15 days. Sensitivity analysisRevMan software carried out sensitivity analysis to reflect the sensitivity of the literature through the change of effect size after deleting one of the literatures. Country(ies) involvedChina.
部分急性心肌梗死患者在及时经皮冠状动脉介入治疗开通狭窄血管后,仍会发生左心室重构的相关问题,包括左心室解剖形态改变、容积扩大、功能减低、继发二尖瓣反流和左心房扩大等,以上各因素密切关联,相互作用,继而影响患者预后.超声心动图作为心血管影像检查一线方法,可对急性心肌梗死后左心室重构及相关情况进行详细评估和定期监测,本文分析总结了超声心动图近年来评估左心室重构的相关方法和进展.
巨噬细胞的趋化聚集与慢性炎症性疾病的发展密切相关.目前药物治疗炎症性疾病的局限是药物半衰期短,生物利用度差.透明质酸作为一种具有良好生物相容性的分子,可以与活化后巨噬细胞表面的CD44分子特异性结合,通过透明质酸制备的纳米颗粒可以靶向炎症病灶中的巨噬细胞,达到治疗炎症性疾病的目的.本文旨在对含透明质酸的纳米颗粒靶向巨噬细胞治疗炎症性疾病的临床前研究作一综述,以期为炎症性疾病的药物精准治疗提供参考.
目的 通过小鼠胚胎面颅三维计算机断层扫描重建技术,结合成骨相关基因检测系统分析2,3,7,8-四氯代二苯并[b,e][1,4]-二噁英(TCDD)对面颅骨发育的致畸作用.方法 将孕鼠分为对照组和实验组,于小鼠胚胎期第12.5天,使用TCDD(玉米油配制)按40 μg/kg对实验组孕鼠单次灌胃,对照组单次灌胃等体积玉米油.收集胚胎期第13.5天和第14.5天胎鼠头颅,组织切片检测成骨相关基因的表达情况.收集两组胚胎期第18.5天小鼠胚胎头颅进行显微计算机断层扫描并三维重建,将面颅骨分块测量、对比分析.结果 TCDD造成小鼠多种面颅畸形,包括颅缝早闭、上颌骨及腭骨水平短小、翼腭突畸形及下颌下降不足.实验组小鼠下颌骨的宽度和体积明显减少,腭突的水平生长明显受损,导致垂直延伸过度,腭中缝宽大,形成腭裂.实验组小鼠腭突内,成骨分化相关基因Runx2、Osterix、Col1a1、Bmp2和Bmp4表达下调.实验组小鼠腭突内芳香烃受体水平升高,雌激素受体水平下降.结论 TCDD能显著抑制小鼠面颅骨发育,特别是造成颅缝早闭和腭骨成骨抑制,该效应与多种成骨相关基因的异常表达相关.
