Using inducers to induce cells to produce inflammatory response is a common in vitro experimental method to study inflammation.However,there are relatively few inflammatory models developed for the cosmetic industry,and there are also great differences in the control of model induction,the selection of inflammatory indicators,and the concentration of inducers.Therefore,in this paper,by systematically studying the effects of Lipopolysaccharide(LPS)on the cell viability,the levels of IL-1α,IL-8 and ROS of human primary keratinocytes,a skin inflammation model based human primary keratinocyte was developed.The results showed that 0.01～100 µg/mL LPS had no significant effect on the cell viability of human primary keratinocytes,while 100 µg/mL LPS could simultaneously induce human primary keratinocytes to produce large amounts of IL-1α and IL-8,and 0.01 µg/mL LPS could induce plentiful ROS.Therefore,a skin inflammation model for differential induction of different inflammatory indicators was established,and the sample OSM2021041301 was tested with this model,it was found that sample OSM2021041301 could significantly inhibit LPS-induced elevated IL-1α and IL-8 levels,the inhibitory effect on LPS-induced elevated ROS level was weak.The results indicated that OSM2021041301 has certain anti-inflammatory effect on inflammation caused by the increase of IL-1α,IL-8 and ROS induced by LPS and its analogues.
On April 10, 2023, the Shanghai Municipal Medical Products Administration issued a notice on the public consultation of the "Shanghai Pudong New Area General Cosmetics On-site Personalized Service Review Rules (for trial implementation)" (draft for comment), marking the public call for comments from April 3 to May 2, 2023. This is another effective regulatory measure following the Shanghai Pudong New Area in June 2022 and the National Medical Products Administration in November to further explore a viable mode for personalized cosmetic services.
May 23rd, 2023, Center for Medical Device Evaluation, National Medical Products Administration introduced Guidelines for the Registration and Review of Recombinant Collagen Wound Dressings (hereinafter referred to as Wound Dressings Guidelines) and Guidelines for Raw Material Evaluation of Recombinant Humanized Collagen (hereinafter referred to as Raw Material Guidelines), which could better standardize the management of the raw material of recombinant collagen and wound dressings. It is worth mentioning that Raw Material Guidelines mainly offers the evaluation methods concerning the raw material of recombinant humanized collagen used by medical devices. Currently, in the medical beauty and cosmetics industry, many products adopt recombinant humanized collagen. These two guiding principles exert an effect on standardizing medical beauty device products, and also have a certain guiding significance for cosmetics added with recombinant humanized collagen.
In order to search an appropriate evaluation method for the soothing effect of cosmetics,the LPS-induced macrophage model,SDS-stimulated zebrafish embryos model and the clinical assessment were used.The results showed that the concentration of NO and IL-6 was significantly reduced in LPS-induced RAW264.7 cells when applied to two essences.The inhibition rate of NO-release was 85.87％ and 84.57％,while IL-6 was 44.18％ and 38.76％,respectively.Furthermore,the spontaneous rotating of zebrafish embryos was significantly decreased to 51.85％ and 76.54％ in SDS-stimulated zebrafish embryos model.In the 28 days of clinical assessment,the concentration of hydration was significantly increased to 55.56％ and 39.61％,while the erythrodesis was significantly decreased to 4.75％ and 6.68％.This indicates that the three evaluation methods of soothing efficacy in this research were effective,and can be used in the evaluation of cosmetics.
The effect of triethyl citrate(TEC)on the stability of cream preparations containing urea or hydroxyethyl urea along with their moisturizing effect on skin was investigated.The results showed that creams incorporating urea or hydroxyethyl urea led to increase in pH value after a long-term and high-temperature storage.pH change in the latter one was relatively smaller.With the addition of TEC,the elevated pH of the formula system was suppressed.In the situation of stable formula,the higher the TEC added,the more significant inhibition effect on the pH change was observed.In the moisture retention test in vivo,compared to 27.19％ moisture rate in negative control,cream preparations containing 5％ urea or 5％ hydroxyethyl urea had remarkable moisturizing effect,which were all higher than 52％.The latter one was relatively more prominent.There is no significant effect on moisturizing effect for product with TEC additive.
