Objective:To investigate the research hotspots and trends in acupuncture treatment for postoperative pain in anorectal diseases based on CiteSpace,aiming to provide basis for clinical prevention and relative research.Methods:The relevant literature of postoperative pain in anorectal diseases treated by acupuncture were retrieved in the databases of CNKI,Wanfang Data,VIP and Web of Science(WOS)from the establishment to 31st December,2022.CiteSpace was used for bibliometrics analysis.Results:Finally,326 relevant articles from Chinese database and 4 relevant articles from WOS were included in the present study.The authors with the largest number of literature from Chinese database included HE Jianping,ZHANG Xitao and ZHANG Min;the authors of literature from WOS included WU JY,ZHAO Y,QIN D and Yeh MeiLing.The institutions with the highest number of articles from Chinese database included Guang'anmen Hospital of China Academy of Chinese Medical Sciences.The country with the highest number of publications in WOS was China,and China also has the highest centrality.High-frequency keywords in Chinese literature include pain,postoperative pain,mixed hemorrhoids,acupoint application,and acupoint embedding.High-frequency keywords in the WOS included hemorrhoids,electro-acupuncture,complementary therapy,analgesia,and acupuncture.Chinese keywords form 10 clusters,with 14 prominent keywords identified,primarily including postoperative anal fistula,post-surgery,anorectal diseases,acupoint pressing,and Baihuanshu(BL30).Conclusion:In clinical research on acupuncture for postoperative pain management in anorectal diseases,early studies focused primarily on pain relief.This was followed by investigations into surgical techniques aimed at reducing postoperative pain in anorectal diseases.In recent years,acupuncture has become a mainstream approach for managing postoperative pain in this field,accompanied by an increase in high-quality controlled trials and clinical studies.However,there remains a notable lack of research on the underlying mechanisms and basic studies,which is anticipated to be a major focus of future research.
Objective:To observe the effect of moxibustion preconditioning on NLRP3/Caspase-1/GSDMD pathway of gastric mucosal cells in rats with acute gastric mucosal injury.Methods:30 healthy SPF SD rats were divided into the normal group,the model group and the moxibustion preconditioning group.Zusanli(ST36,bilateral)and Zhongwan(RN12)were selected for moxibustion preconditioning for 7 days,and then the acute gastric mucosal injury model was established by gastric perfusion with anhydrous ethanol.The morphology of gastric mucosa was observed,UI index was evaluated,ELISA method was used to detect the expressions of TNF-α,IL-1β and IL-18 in gastric tissues.Western blot method was used to detect the expressions of NLRP3,Caspase-1 and GSDMD in gastric tissues.Results:After moxibustion preconditioning,the body could inhibit the secretions of IL-1β,IL-18 and TNF-α in response to the stimulation of pathogenic factors,when compared with those in the normal group and the model group(P＜0.01).The expressions of NLRP3,Caspase-1 and GSDMD were lower in the moxibustion preconditioning group than those in the normal group and the model group(P＜0.01).Conclusion:Moxibustion preconditioning can inhibit pyroptosis through NLRP3/Caspase-1/GSDMD classical pathway,alleviate inflammatory response to achieve the role of protecting gastric mucosa.
ZHOU Meisheng made a detailed summary of the transmission rules of moxibustion sensation in the process of moxibustion therapy in Three Phases of Moxibustion Sensation.This has an important guiding significance for the clinical diagnosis and treatment with moxibustion therapy in later generations.This study took'stimulating sensation transmission'as the entrance point,combined the law of sensation transmission of'three-phases of moxibustion sensation',and further analyzed the relevant elements including moxibustion points and moxibustion quantity,in order to expect that the idea of'sensation transmission'can be paid more attention to and further be studied in terms of moxibustion.
Objective:To observe the efficacy of Lingnan back acupuncture in treatment of benign prostatic hyperplasia(BPH)of Kidney-Yang deficiency.Methods:84 patients with BPH were randomly divided into the observation group and the control group,with 42 cases in each group.The control group was treated with conventional acupuncture and the observation group was treated with Lingnan back acupuncture.International prostate symptom(IPSS)score,quality of life(QoL)score,maximum urine flow rate(Qmax)and residual urine volume(RU)were observed before and after the treatment in the two groups.Clinical efficacy was compared between the two groups.Results:After 4 courses of the treatment,the IPSS score,QoL score and RU were lower(P＜0.05,P＜0.01),and Qmax was higher than those before the treatment in the two groups(P＜0.05);of which the improvements were more significant in the observation group(P＜0.05).The total effective rate of the observation group was 75.00％(30/40),which was higher than 56.41％(22/39)of the control group(P＜0.05).There were no serious adverse reactions in both groups.Conclusion:Lingnan back acupuncture is superior to conventional acupuncture in treatment of BPH of Kidney-Y ang deficiency,and has a significant efficacy.It can improve symptoms of urethral obstruction,reduce frequency of urination,promote recovery of urinary function,improve sexual function and quality of life,and has a high safety and a clinical promotion significance.
Objective:To analyze the effect of motor imaginary acupuncture combined with transcranial electrical stimulation on cerebral hemodynamics and limb function of hemiplegic patients after stroke with the syndrome of Qi deficiency and blood stasis.Methods:168 patients with post-stroke hemiplegia due to Qi deficiency and blood stasis were randomly divided into the control group,group A,group B and group C,with 42 patients in each group.The control group was treated with conventional rehabilitation,group A was treated with conventional rehabilitation plus transcranial electrical stimulation,group B was treated with conventional rehabilitation plus motor imaginary acupuncture,and group C was treated with conventional rehabilitation,transcranial electrical stimulation and motor imaginary acupuncture.The symptom scores,clinical curative effect,cerebral hemodynamics,limb function and adverse reactions were compared among the four groups before and after the treatment.Results:After the treatment,the scores of main symptoms and secondary symptoms,as well as the total scores were decreased in the four groups(P＜0.05),and the scores of group A,group B and group C were lower than those of the control group(P＜0.05);the scores of group C were lower than those of group A and group B(P＜0.05).There was a statistically significant difference in the distribution of clinical curative effect among the four groups(P＜0.05),and the total effective rate of group C was higher than that of the control group(P＜0.05).After the treatment,the blood flow velocities of middle cerebral artery(MCA),anterior cerebral artery(AC A),posterior cerebral artery(PC A)and basilar artery(BA)and the functional scores of upper and lower limbs were increased in the four groups(P＜0.05),which were higher in group A,group B and group C than those in the control group(P＜0.05);and the blood flow velocities and the functional scores were higher in group C than those in group A and group B(P＜0.05).There was no statistical difference in the incidences of adverse reactions among the four groups(P＞0.05).Conclusion:Both motor imaginary acupuncture and transcranial electrical stimulation can reduce symptoms,enhance efficacy and improve cerebral hemodynamics and limb function in treatment of post-stroke hemiplegia with the syndrome of Qi deficiency and blood stasis.The combination of the two has stronger effect and better efficacy,which is safety.
Hypertension is a major risk factor for cardiovascular events and widely affects the morbidity and mortality of related diseases worldwide.As one of the key parts of the complex mechanism of hypertension,immune-inflammatory response includes adaptive immunity and innate immunity,both of which play an important role in the occurrence and development of hypertension.The previous studies on immune-inflammatory response showed that acupuncture could reduce the levels of inflammatory factors in the state of hypertension and reduce the inflammatory infiltration of target organs.It can reduce the degree of myocardial hypertrophy caused by hypertension and improve vascular remodeling.The imbalance of TH17/Treg cells was corrected to maintain immune homeostasis.This study summarized the scientific evidence of immune-inflammatory response in terms of acupuncture for hypertension,and found that acupuncture could focus on NETs of neutrophils,crosstalk between intestinal flora and DCs,which provided a direction for clarifying the clinical significance of acupuncture in treatment of hypertension.
This study summarized the clinical and experimental reports on acupoint-specific functional magnetic resonance imaging(fMRI)in the past 20 years from the aspects of single-acupoint fMRI studies,acupoint compatibility effect mechanism studies,true-false acupoint and true-false needling fMRI studies,acupuncture effect influence factors studies,post-acupuncture effect fMRI studies,acupuncture mechanism fMRI studies,acupuncture fMRI influence factors,moxibustion effect fMRI studies and animal experimental fMRI studies.The purpose of this study is to provide a visual imaging basis for the central mechanism of acupuncture and to provide guidance for acupuncture points selection.It is found that fMRI technology has its own advantages,but also has many shortcomings,and the design of future clinical trials should be enhanced with standardization and scientificity.
Ischemic stroke is a common cardiovascular and cerebrovascular disease with high morbidity,high mortality and high disability rate.The combination of acupuncture and medication has certain advantages in improving the neurological damage of ischemic stroke.This study summarized the literature on treatment of ischemic stroke with acupuncture and medication in recent years,and mainly discussed the treatment methods and their mechanisms.It is found that the combination of acupuncture and medication has a more significant effect than the simple application of acupuncture or medication in treatment of ischemic stroke.It can accelerate the repair of hippocampal neurons,effectively repair free radical damage,reduce inflammatory response and regulate NO and ET levels,and regulate function of ion channels,which highlights the therapeutic advantages of the combination of acupuncture and medication.
Objective:To observe the clinical effect of meridian sinew therapy of Zhuang medicine in treatment of simple obesity and to explore its mechanism.Methods:66 patients were randomly divided into the treatment group and the control group,with 33 cases in each group.Finally,31 cases were completed in the treatment group and 32 cases in the control group.The treatment group was treated with meridian sinew therapy of Zhuang medicine,including massage resolving knot,acupuncture resolving knot and cupping resolving knot.The control group was treated with traditional acupuncture and cupping.TCM syndrome score and obesity index were compared among before the treatment,after the treatment and during the follow-up in the two groups.The serum contents of fatty cytokine and inflammatory factors were detected before and after the treatment in the two groups.Results:The total effective rate was 93.55％(29/31)in the treatment group,which was higher than 71.88％(23/32)in the control group(P＜0.05).The TCM syndrome score,the body mass index(BMI),the body mass,the waist circumference,the waist-hip ratio(WHR)and the body fat rate(F％)were lower after the treatment and during the follow-up than those before the treatment in the two groups(P＜0.05);in which there were statistical differences between the two groups(P＜0.05).The levels of leptin,free fatty acid(FFA),Vaspin,resistin,interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were lower(P＜0.05),and adiponectin was higher after the treatment than those before the treatment in the two groups(P＜0.05);of which the changes were more significant in the treatment group than those in the control group(P＜0.05).Conclusion:Meridian sinew therapy can improve the degree of obesity,adjust fat cell activity factors,ameliorate glucose and lipid metabolisms,and inhibit inflammation in treatment of simple obesity.
Objective:To explore the acupoints selection rule of acupuncture and moxibustion in treating cancer-related depression(CRD)by using data mining techniques.Methods:The clinical research literature on acupuncture and moxibustion for CRD was retrieved by searching the databases of CNKI,Wanfang,VIP,PubMed and SinoMed from the establishments to September 30,2022.Excel 2010 software was used to establish the database of selected acupoint prescriptions,and the frequency use of acupoints was counted,SPSS Modeler 18.0 software was used for association rule analysis of prescriptions,SPSS 22.0 software for cluster analysis and Gephi 0.9.1 software for complex network analysis.Results:A total of 71 literature contained 83 acupuncture prescriptions involving 79 acupoints,and the total frequency of acupoints used was 582 times.Zusanli(ST36),Neiguan(PC6),Sanyinjiao(SP6),Taichong(LR3)and Hegu(LI4)were the top five acupoints used in treatment of CRD by acupuncture and moxibustion,and the acupoints used were mostly distributed in the lower extremities,with the highest frequency of acupoints attributed to foot-Taiyang meridian,and Baihui(DU20)-Yintang(EX-HN3)and LI4-ST36 were the high-frequency pairs for treating CRD.Conclusion:Clinical acupuncture treatment for CRD focuses on acupoints selection based on identification of internal organs,and the combination of distant points,local points and meridian points,with the core points of ST36,PC6,SP6,LR3 and LI4.The combination of acupoints is flexibly modified to activate Du meridian and clear mind,relieve depression and calm Shen based on syndrome differentiation to achieve therapeutic effects.
Objective:To investigate the efficacy of Jiantong point balancing acupuncture combined with needle-warming moxibustion in treatment of periarthritis of shoulder.Methods:A total of 123 patients with periarthritis of shoulder were randomly divided into the control group(n=63)and the observation group(n=60).The control group was treated with needle-warming moxibustion;on which basis,the observation group was also treated with Jiantong point balancing acupuncture.Clinical efficacy was compared between the two groups.Constant-Murley score,VAS score,MBI score,TCM symptom scores and serum levels of TGF-β1 and PGE2 were compared between before and after the treatment in the two groups.Results:The curative effect of the observation group was significantly higher than that of the control group(P＜0.01).There were no statistical differences in the symptom scores of tenderness,mobility restriction and pain between the two groups before the treatment(P＞0.05);the symptom scores were significantly decreased after the treatment in the two groups(P＜0.01),and the decreases were more significant in the observation group(P＜0.01).There was no statistical difference in Constant-Murley score between the two groups before the treatment(P＞0.05);which was significantly increased after the treatment in the two groups(P＜0.01),and the increase in the observation group was greater than that in the control group(P＜0.01).There were no statistical differences in VAS score and MBI score between the two groups before the treatment(P＞0.05);which were significantly improved after the treatment in both groups(P＜0.01),and the improvements in the observation group were more significant(P＜0.01).There was no statistical difference in the range of motion(ROM)of shoulder between the two groups before the treatment(P＞0.05);after the treatment;the abductor,extension and flexion motion of shoulder joint were significantly increased in the two groups(P＜0.01),and the increases were more remarkable in the observation group(P＜0.01).There were no statistical differences in the serum levels of TGF-β1 and PGE2 between the two groups before the treatment(P＞0.05);after the treatment,the serum levels of TGF-β1 and PGE2 were significantly decreased in the two groups(P＜0.01),and the decreases were more significant in the observation group(P＜0.01).Conclusion:Jiantong point balancing acupuncture combined with needle-warming moxibustion has a significant effect in treating periarthritis of shoulder,which is worthy of clinical application.
Objective:To observe the changes of ethology and the diversity,composition and function of gut microbiota,so as to explore the potential mechanism of'Tongdu Qishen'(dredging Du meridian and awaking Shen)acupuncture in improving cognitive dysfunction of APP/PS1 mice.Methods:APP/PS1 mice were randomly divided into the model group,the acupuncture group and the probiotics group.C57BL/6 mice were used as the normal control.In the acupuncture group,Baihui(DU20),Yintang(DU29)and Zusanli(ST36)were selected for needling.The probiotics group was treated with probiotics.The above interventions were administered once a day for 56 consecutive days.Morris water maze was used to evaluate the spatial learning and memory ability of mice.The 16S rRNA high throughput sequencing was used to detect the diversity,composition and function of gut microbiota in mice.Results:Compared with those in the normal group,the escape latency was significantly increased(P＜0.01);the platform crossover number and the percentage of time and swimming distance were substantially decreased(P＜0.01);the diversity of gut microbiota was notably reduced,and the composition and function of gut microbiota were significantly changed in the model group(P＜0.01).These changes were reversed in the acupuncture group and the probiotics group(P＜0.01,P＜0.05).Conclusion:'Dongdu Qishen'acupuncture can benignly regulate the dysbiosis of gut microbiota,thus to effectively enhance the learning and memory ability in the APP/PS1 mice.
Objective:To observe the clinical effect of Xingnao Kaiqiao acupuncture combined with Bobath technique in treating post-stroke shoulder hand syndrome(SHS)of stage Ⅰ.Methods:60 patients with post-stroke SHS of stage Ⅰ were randomly divided into the control group and the observation group,with 30 patients in each group.On the basis of routine treatment in internal medicine,the control group was treated with routine acupuncture combined with Bobath technique,whereas the observation group was treated with Xingnao Kaiqiao acupuncture combined with Bobath technique.Both groups were treated for 30 min each time,once a day,5 times a week for a total of 4 weeks.Visual pain simulation(VAS)score,hand swelling score,upper limb simplified Fugl-Meyer Motor Function Scale(FMA-UE)score and modified Barthel Index Scale(MBI)score were observed and compared between before and after the treatment in the two groups.Results:The VAS score and hand swelling score were lower(P＜0.01),and FMA-UE score and MBI score were higher after the treatment than those before the treatment in the two groups(P＜0.01);the observation group was better than the control group in improving the flexibility of affected limb,pain level and hand swelling degree(P＜0.05);there was no difference in the ability to perform activities of daily living between the two groups(P＞0.05).Conclusion:Xingnao Kaiqiao acupuncture combined with Bobath technique has an effective therapeutic effect on post-stroke SHS of stage I,which can effectively relieve muscle pain and swelling of affected shoulder,elbow and hand joint and improve flexibility of affected limb.Its efficacy is better than that of ordinary acupuncture combined with Bobath technique.
Objective:To explore the clinical efficacy of ultrasound-guided acupuncture combined with extracorporeal shock wave in treatment of external humeral epicondylitis.Methods:98 patients with external humeral epicondylitis were randomly divided into the observation group(n=49)and the control group(n=49).The control group was treated with extracorporeal shock wave;on which basis,the observation group was also treated with ultrasound-guided acupuncture.The levels of hs-CRP,IL-17 and IL-8,joint swelling index,joint activity index,the scores of VAS,HSS and SF-36 were observed before and after the treatment in the two groups.The clinical efficacy was compared between the two groups.Results:There were no statistical differences in the levels of hs-CRP,IL-8 and IL-17 between the two groups before the treatment(P＞0.05),which were significantly decreased in the observation group compared with those in the control group after the treatment(P＜0.05).There were no statistical differences in the joint swelling index and the joint activity index between the two groups before the treatment(P＞0.05),which were remarkably decreased in the observation group compared with those in the control group after the treatment(P＜0.05).There were no statistical differences in the scores of VAS and HSS between the two groups before the treatment(P＞0.05);the score of VAS was lower and the score of HSS was higher in the observation group than that in the control group after the treatment,respectively(P＜0.05).SF-36 presented no statistical difference between the two groups before the treatment(P＞0.05),which was higher in the observation group than that in the control group after the treatment(P＜0.05).The total effective rate was 95.92％(47/49)in the observation group,which was slightly higher than 85.72％(42/49)in the control group,with no statistical difference(x2=3.058,P=0.080).Conclusion:Ultrasound-guided acupuncture combined with extracorporeal shock wave can effectively improve clinical efficacy,attenuate inflammation response,alleviate pain,promote recovery of joint function and improve quality of life in treatment of external humeral epicondylitis.
Objective:To observe clinical efficacy of heat-reinforcing needling combined with Methotrexate in treatment of early rheumatoid arthritis(RA).Methods:64 cases of early RA met the diagnostic criteria were randomly divided into the heat-reinforcing needling group(n=32)and the western medication group(n=32).The western medication group was treated with Methotrexate Tablet,5 mg,once a week;on which basis,the heat-reinforcing needling group was also treated with heat-reinforcing needling method,once every other day,three times a week.After 12 weeks of treatment,clinical efficacy was compared between the two groups.The total score of TCM symptoms and DAS28 index,as well as the contents of CRP,ESR,RF and anti-CCP antibody were detected before and after the treatment in the two groups.Results:The total effective rate was 90.62％(29/32)in the heat-reinforcing needling group,which was significantly higher than 75.00％(24/32)in the western medication group(P＜0.05).The total score of TCM symptoms,DAS28 index,as well as the levels of CRP,ESR,RF and anti-CCP antibody were significantly decreased after the treatment compared with those before the treatment(P＜0.05),which were lower in the heat-reinforcing needling group than those in the western medication group(P＜0.05).Conclusion:The combination of heat-reinforcing needling and Methotrexate has a significant therapeutic effect in treatment of early RA of wind-dampness blockage syndrome,it can shorten the course of treatment and reduce the adverse reactions of the western medication.
Objective:To investigate the clinical effect of Mulligan manipulation combined with floating needling in treatment of cervical spondylosis of vertebral artery pattern.Methods:78 patients with cervical spondylosis of vertebral artery pattern were randomly divided into the control group(n=37)and the combined group(n=41).The control group was treated with Mulligan manipulation;on which basis,the combined group was also treated with floating needling.Clinical effective rate,adverse reactions,cervical vertigo symptom and function assessment scale(ESCV),function scale of cervical spondylosis(vertebral artery pattern)assessment(FS-CSA),mean blood flow velocity(Vm),resistance index(RI),C-reactive protein(CRP)and erythrocyte sedimentation rate(ESR)of vertebral artery and basilar artery were compared between the two groups before and after the treatment.Results:The clinical effective rate of the combined group was significantly higher than that of the control group(P＜0.05).ESCV,Vm of vertebral artery and basilar artery were higher after the treatment than those before the treatment in the two groups;which were higher in the combined group than those in the control group after the treatment(P＜0.05).FS-CSA,RI,CRP and ESR of vertebral artery and basilar artery were lower after the treatment than those before the treatment in the two groups,which were lower in the combined group than those in the control group after the treatment(P＜0.05).There were no adverse reactions in both groups during the treatment.Conclusion:Mulligan manipulation combined with floating needling has a significant effect on cervical spondylosis of vertebral artery pattern,which can improve blood supply and circulation,inhibit inflammation,and relieve pain and dizziness of the patients.
This study introduced professor GUAN Ling's puncturing collaterals techniques with her modified structure-based acupuncture in treatment of allergic diseases.Based on the theory of classical acupuncture and moxibustion,professor GUAN Ling combined the relevant knowledge of modern anatomy and physiology and took blood vessels as the starting point to explore and sort out the puncturing collaterals technique,which can treat various clinical diseases by stimulating the blood vessel walls to cause sympathetic nerve effect.Its application in allergic diseases was explored in this study.
Objective:To observe the changes of phenotypic markers PLN and MGP of vascular smooth muscle cells(VSMCs)in rats with acute cerebral infarction(ACI),and to explore the intervention effect of electro-acupuncture(EA).Methods:60 healthy male Wistar rats were randomly divided into the blank group,the sham operation group,the model group and the EA group.There were 6 rats in the blank group and in the sham operation group,and the model group and the EA group were further subdivided into 4 time phases at 3 h,6 h,24 h and 72 h after the operation,with 6 rats in each time phase.The model of permanent middle cerebral artery occlusion(MCAO)was prepared by modified Longa intracavitary suture method.The EA group was stimulated with 2/15 Hz and 2 mA electric wave for 20 min at Shuigou(DU26).The 3 h,6 h and 24 h groups were intervened once immediately after modeling,and the 72 h group was intervened once a day.The rats in the model group,the sham operation group and the blank group were captured and fixed at corresponding time points.NSS scoring method was used to evaluate the neurological impairment of the rats at different phases in each group.Immunohistochemical method and Western Blot method were used to detect the changes of phenotypic marker proteins PLN and MGP in VSMCs of right middle cerebral artery at acute stages(3 h,6 h,24 h,72 h),and the effect of EA intervention was observed.Results:After MCAO,the NSS scores of the model group and the EA group showed a gradual decreasing trend with the extension of ischemia time,and the NSS scores of each phase in the EA group were lower than those of the simultaneous phase in the model group,with a statistical significance at 72 h(P＜0.05).There was no statistical difference in the positive expressions and the protein expressions of PLN and MGP between the sham operation group and the blank group(P＞0.05).PLN positive expression,MGP positive expression and MGP protein expression in the model group and the EA group were statistically significant compared with those in the blank group and the sham operation group at each phase(P＜0.05,P＜0.01).PLN protein expressions in the model group and the EA group were statistically significant compared with those in the blank group and the sham operation group at 24 h and 72 h(P＜0.05,P＜0.01);the positive expression of PLN in the middle cerebral artery of the EA group was higher than that of the simultaneous phase in the model group at acute stage(P＜0.01),but there was no statistical difference in the protein expression of PLN between the two groups(P＞0.05).The positive expression of MGP in the middle cerebral artery of the EA group was lower than that of the model group at the same time during acute stage(P＜0.01),and the MGP protein expression in the EA group and the model group was statistically significant at6h(P＜0.05).Conclusion:Electro-needling DU26 can significantly inhibit the abnormal phenotypic transformation of VSMCs,so as to make the cerebral vessels in a normal state of contraction phenotype.
Objective:To investigate the clinical intervention effect of different acupuncture time and frequency parameters to stimulate specific acupoints on patients with upper motor neurogenic intestinal dysfunction.Methods:60 patients were randomly divided into group A,group B,group C and group D,with 15 cases in each group.The points of Zhigou(SJ6),Dachangshu(BL25),Zusanli(ST36),Ciliao(BL32),Qihai(RN6),Changqiang(DU1),Tianshu(ST25)and Shangjuxu(ST37).After obtaining Qi feeling,ST25,BL25,ST36 and BL32 were connected to electrodes.Wave pattern,wave frequency,electro-acupuncture(EA)time and EA duration were as follows in each group;continuous wave,5Hz,Chen hour(7am-9am),20 minutes in group A;continuous wave,5Hz,not Chen hour,20 min in group B;continuous wave,100 Hz,Chen hour,20 min in group C;continuous wave,100Hz,not Chen hour,20 min in group D.International spinal cord injury basic intestinal function data set,Bristol stool typing,residual number of large intestine markers in intestine and generic quality of life inventory-74(GQOL-74 scale)were compared before and after the treatment in all groups.Results:Compared with those before the treatment,the scores of international spinal cord injury basic intestinal function data set,Bristol stool typing,intestinal residual number of large intestine markers and GQOL-74 scale of the patients were all improved after the treatment in all groups(P＜0.05).Among them,the improvements in the scores of international spinal cord injury intestinal function basic data set,intestinal residual number of large intestine markers and GQOL-74 scale were more significant in group C than those in group A,group B and group D(P＜0.05);and the improvements were more significant in group A and group D than those in group B(P＜0.05);there were no statistical differences in the improvements between group A and group D(P＞0.05).In terms of Bristol stool typing,the improvement was more significant in group C than that in group A,group B and group D(P＜0.05),and there was no statistical difference among group A,group B and group D(P＞0.05).Conclusion:In terms of the treatment for upper motor neurogenic intestinal dysfunction,high-frequency EA during Chen hour has the best curative effect,which can effectively relieve the symptoms of defecation time,dry stool and difficult defecation,improve transmission function of colon,and improve life quality of the patients.The curative efect of the therapy is clear and the therapy is worthy of clinical promotion.
Objective:To investigate the effects of electro-acupuncture(EA)combined with Pantoprazole on immune function,pathological morphology and expression of CYP17/CYP19 in nude mice with breast cancer.Methods:45 nude mice were divided into the control group(n=9),the model group(n=8),the medication group(n=9),the acupoints treatment group(n=8)and the combination group(n=8).After successful modeling of breast cancer,the medication group was given Pantoprazole gavage,the acupoints treatment group was treated with electro-needling Baihui(DU20)and Changqiang(DU1),and the combination group was treated with Pantoprazole gavage and electro-needling DU20 and DU 1.The serum levels of estrogen,NK cells and T lymphocyte receptors were observed.The tumor mass of nude mice was calculated.HE staining was used to observe the pathological morphology of tumor tissues.TUNEL method was used to detect the apoptosis rate of tumor cells in each group.Immunoblotting method was performed to detect the proteins of CYP17,CYP19,Bcl-2 and Bax in tumor tissues.Results:Compared with those in the control group,the serum levels of E2,P,PR,LH,FSH,CD3+,FOXP3+,CYP17,CYP19,Bcl-2 were increased,TCRαβ+,CD161a+antibody,T lymphocyte CD3+CD4+,CD3+CD8+and Bax were decreased in the model group(P＜0.05).Compared with those in the model group,the serum levels of E2,P,PR,LH,FSH,CD3+,FOXP3+,CYP17,CYP19 and Bel-2 were decreased,and TCRαβ+,CD161a+antibody,T lymphocytes CD3+CD4+,CD3+CD8+and Bax were increased in the medication group and the acupoints treatment group(P＜0.05);in which there were statistical differences between the acupoints treatment group and the combination group(P＜0.05).The tumor mass was(0.00±0.00)g,(0.42±0.06)g,(0.30± 0.05)g,(0.28±0.06)g and(0.21±0.03)g in the control group,the model group,the medication group,the acupoints treatment group and the combination group,respectively;in which there were statistical differences among the groups(F=78.340,P＜0.05).The apoptosis rate of tumor cells was(0.00± 0.00)％,(3.51±0.71)％,(24.12±5.22)％,(24.28±6.22)％and(45.78±8.44)％in the control group,the model group,the medication group,the EA group and the combination group,respectively;in which there was statistical difference among groups(F=106.100,P＜0.05).In the model group,the mammal gland structure disappeared,the lobule increased,the duct expanded,and the glands changed like tumors.Compared with those in the model group,lobular structure and duct hyperplasia were improved in the medication group and the acupoints treatment group;in the combination group,the proliferation and dilation of mammary duct were improved,the lobular acinus was decreased,and the degree of tumorous change was weakened.Conclusion:EA combined with Pantoprazole can significantly improve estrogen levels and immune function,reduce tumor quality and improve tumor-like changes in nude mice with breast cancer,and the mechanism may be related to inhibiting the levels of CYP17 and CYP19.
