Abstract To assess the in vitro effect of experimental solution and dentifrice on the progression of erosive tooth wear. Bovine enamel (n = 40) and root dentin (n = 40) specimens were pre-eroded (0.3% HCl; pH 1.2; 5 min) and randomized into 4 groups (n = 10, per group): experimental solution based on mesoporous silica containing calcium and fluoride (MSCaNaF); experimental dentifrice based on magnesium and fluoride (MgF2) and negative (water, Milli-Q®) and positive (stannous-containing dentifrice, Elmex®) controls. After half-covering, the specimens were submitted to a second erosive challenge for 5 days (100 μL; 1min; 3×/day). Differences in volumetric roughness (Sa) and tooth structure loss (TSL) by use of 3D noncontact optical profilometry were evaluated. Scanning electron microscopy (SEM) was performed. The data were analyzed by Kruskal-Wallis, Mann-Whitney, and Wilcoxon tests (α=0.05). The intra-group evaluation of Sa showed that all groups differed from the initial condition. For Sa, in relation to enamel and dentin, all experimental groups showed a difference in roughness compared with the negative control (p < 0.05). TSL analysis revealed that the MSCaNaF, MgF2, and positive control groups were similar (p>0.05) and more effective compared to the negative control (p<0.05). In the SEM images, the negative control presented the worst loss of dental structure, with more porous enamel. The dentine surface topography pattern showed partially or totally occluded dentinal tubules after treatments, except in the negative control group. The new products based on MSCaNaF and MgF2 are effective in controlling the progression of dental erosion, similar to commercial products
Abstract This study evaluated the pH, solubility, antimicrobial activity, and bond strength of a calcium hydroxide intracanal medication (80% calcium hydroxide and 20% zirconium oxide in polyethylene glycol 400 vehicle), evaluated alone or combined with 20% F18 bioactive glass or copper-doped F18 bioactive glass. Polyethylene tubes containing the medications were immersed in distilled water. pH (n=10) was measured after 1, 3, 7, 14, and 21 days, and solubility (n=7) was determined after 7 and 14 days. Antimicrobial activity (n=6) was assessed using a modified direct contact test against Enterococcus faecalis and/or Candida albicans. For bond strength analysis (n=12), bovine roots were filled with the medications for 14 days and subsequently obturated with Bio-C Sealer or AH Plus. Specimens were subjected to a push-out test, and data were analyzed by ANOVA and Tukey’s test (α=0.05). All formulations exhibited an alkaline pH (p>0.05). The formulation without bioactive glass showed higher solubility (p<0.05). The copper-doped bioactive glass formulation was the most effective against E. faecalis in single- and dual-species biofilms (p<0.05), whereas the formulation without bioactive glass completely eradicated C. albicans (p<0.05). Regarding bond strength, no differences were observed among groups obturated with AH Plus (p>0.05). When Bio-C Sealer was used, the F18 bioactive glass formulation exhibited the highest bond strength to dentin (p<0.05). Calcium hydroxide combined with F18 bioactive glass reduced solubility and improved the bond strength of Bio-C Sealer, whereas copper-doped bioactive glass enhanced antimicrobial performance, supporting their potential clinical applicability.
Abstract This study reported preliminary clinical outcomes of a randomized clinical trial comparing immediate and conventional loading protocols for mandibular overdentures supported by two narrow-diameter implants with a regular platform manufactured by additive technology. The focus was on primary implant stability, assessed using insertion torque and the implant stability quotient (ISQ). Sixteen completely edentulous participants (11 men, 5 women; mean age: 67.7 ± 7.6 years) received new complete dentures prior to surgery and were followed for up to 180 days, depending on the outcome assessed. Insertion torque was recorded at implant placement (motor and manual wrench), implant stability was measured at baseline and 90 days, and peri-implant healing and health parameters were evaluated at 7, 14, 30, 60, 90, and 180 days. A significance level of 5% was adopted for all statistical analyses. Immediate loading was performed on the day of implant placement, while conventional loading occurred after a 12-week healing period. The immediate loading group showed higher insertion torque (motor: 58.13 ± 5.18 Ncm, p=0.001; wrench: 58.44 ± 4.37 Ncm, p=0.004). ISQ increased significantly from baseline to 90 days in both groups (Immediate: 68.05 ± 6.08 to 72.44 ± 3.38; Conventional: 65.58 ± 5.47 to 70.72 ± 4.63; p<0.001), with no significant between-group differences (p=0.117). Peri-implant healing was more stable in the conventional group, with 100% of bone beds healed at 30 days (p=0.01). Significant between-group differences were observed at selected time points for peri-implant outcomes (p<0.05). Within the limitations of this preliminary short-term evaluation, both protocols appeared clinically feasible.
Abstract The study aimed to assess the prevalence of oral mucosal lesions and periodontal disease in narghile users. A sample of narghile users (n=45) and non-users (n=45), matched by age and sex, was selected from the School of Dentistry at the Federal University of Goiás and tobacconists in Goiânia, Brazil. Demographic and socioeconomic data and characteristics of narghile users were collected through a structured questionnaire. The degree of dependence was assessed using the Fagerström Test for Nicotine Dependence. The median age was 25 (18 - 54) years in users and in non-users, with a predominance of males (80%, n=32). The median duration of use was 6.5 (0.5-33) years. Bleeding on probing was 29.72% (0.6-100) in users and 20.66% (0.2-63.4) in controls (p=0.799). Periodontitis was more frequent in users (n=19; 47.5%) than in controls (n=3; 7.5%) (p<0.001). An association was observed between the degree of dependence and periodontitis (p=0.002). In narghile users, 17 (42.5%) lesions were observed, including two leukoplakia cases. It was concluded that narghile users showed more oral cavity disorders compared to non-users, and an important association was found between the degree of dependence of narghile use and periodontitis
Abstract This study aimed to investigate the anti-biofilm and bactericidal activity of biofunctionalized titanium with either nanohydroxyapatite or F18 bioactive glass against Staphylococcus aureus biofilms. For that, discs (6.0mm diameter and 3.0 mm thickness) with different surface treatments were studied and divided into three groups: 1) machined titanium (M); 2) coated with Nano-hydroxyapatite (Nano-Ha); 3) Biofunctionalized with F18 bioactive glass (BSF18). Surface characterization was investigated through energy-dispersive spectroscopy, scanning electron microscopy, atomic force microscopy, and bioactivity assays. Antibacterial activity was investigated according to the JIS Z 2801:2010 standard and ISO 22196:2011. The samples were evaluated for UFC/mL, biofilm morphology, and confocal laser scanning microscopy was performed after 24 h. The results showed that BSF18 biofunctionalized discs significantly reduced viable bacterial adhesion compared with Nano-Ha and M groups (3.93±0.14; 5.68±0.26; and 5.57±0.16; p<0.0001; respectively). The morphology analysis revealed a smaller number of adhered bacterial cells for BSF18 samples when compared to the M and Nano-HA, additionally, the biofunctionalization of titanium discs with F18 bioactive glass was effective in inhibiting the growth of Staphylococcus aureus biofilm after direct contact for 24 hours analyzed by confocal laser microscopy which demonstrated greater values for dead cells and lesser live cells for BSF18 surface when compared to machined and Nano-HA (p<0.05). F18 bioactive glass can be a promising biomaterial for preventing and controlling infection by S. aureus on titanium implants. It could be a valid infection-preventing strategy for implant dentistry clinical practice.
