Leaf blight disease has become one of the significant ailments in Eucalyptus spp. forest plants. This disease can lead to plant growth disruption and even plant mortality. This research aims to evaluate the antagonistic abilities of several Trichoderma isolates against Pestalotiopsis fungi associated with Eucalyptus leaf blight disease. The research was conducted using the dual culture method, wherein both Trichoderma isolates and the pathogen were cultivated on the same petri dish. The obtained data were analyzed using ANOVA at a 5% significance level, followed by the Duncan test in case of significant differences. The results of BLAST analysis of pathogens causing leaf blight have the highest similarity with Pestalotiopsis uvicola, which is equal to 99.82%. The Results test antagonist show percentage inhibition Trichoderma to pathogen Pestalotiopsis biggest showed by isolate TL122 ie of 65.333%, followed with isolates TL411 and TL631 ie consecutive by 54.49% and 49.33%. The T.L.122 isolate was declared to be the most effective as a potential antagonist fungus against the Pestalotiopsis pathogen which causes leaf blight in Eucalyptus plants.
Cytogenetics is a combination of cytology and genetics which studies the genetic system in cells. Cytogenetic analysis is a combination of cytogenetics (about cells) and genetics which explains the relationship between cellular events (especially chromosomes) and genetic phenomena. The objects observed in cytogenetic analysis are chromosomes. Cytogenetic analysis research has been carried out on kailan plants (Brassica oleraceae L.) with the aim of observing the effective time of mitotic division in kailan plants, the stages of mitotic division, the number and shape of chromosomes in kailan plants (Brassica oleraceae L.). The material used in this research is young roots of the kailan plant. The method used in this research is the squash method. The results of cytogenetic analysis research on kailan plants (Brassica oleraceae L.) showed that the effective time of mitotic division was 08.00 WIB and obtained an overview of the stages of prophase, prometaphase, metaphase, anaphase and telophase, the number of chromosomes of kailan plants (Brassica oleraceae L.) was 2n = 9 pairs of chromosomes or 18 chromosomes, with 5 pairs of metacentric chromosome forms in numbers 2, 3, 4, 5, and 8, and 4 pairs of submetacentric forms in numbers 1, 6, 7, 9.
Laying hens in Indonesia are outstanding and potential commodities. However, Newcastle Disease (ND) is one of the risks that must be faced. Now vaccination is chosen as one of the strategies to prevent ND. This paper aims to examine the effect of inactivated ND vaccine from Lentogenic and Velogenic strains on the increase in antibody titers of laying hens. To find the antibody titer value, the Hemagglutination Inhibition (HI) test method is used with serum sampling time before vaccination, 4th week, 6th week, 8th week, and 10th week post-vaccination. Meanwhile, to see the significance of the difference in titers at each time, a statistical analysis was carried out using the Paired Sample T-test. From this analysis, it is known that the Lentogenic strain has the highest significance at week 8 post-vaccination with a value of 1779.20 compared to 380.80 before vaccination. Meanwhile, the Velogenic strain had the highest significance at week 4 post-vaccination with a value of 1753.60 compared to 143.60 before vaccination. So it can be concluded that the Inactivated ND vaccine is effective in stimulating the formation of protective antibodies to fight against the ND virus attack.
This research is motivated by the fact that the mineral calcium is needed by the body for functional operations and bone health. Calcium needs for body operations include the formation of red blood cells. If blood cell formation is disrupted, it will cause anemia, which can lead to a calcium deficit that can lead to bone loss or what is known as osteoporosis. One of the plants that contain high calcium is mustard greens. The contents of mustard greens include protein, fat, carbohydrates, calcium, phosphorus, iron (Fe), vitamin A, vitamin B and vitamin C. However, green leafy plants such as mustard greens are thought to cause an increase in uric acid in the blood. In this research, mustard juice will be given as calcium intake to fulfill body operations, namely hematocrit levels and hemoglobin levels and blood uric acid levels. 18 white rats are grouped as follows: Group 1 with 4 mL/200g BW, group II 8 mL/ 200g and group III negative control treatment, each treatment was repeated 6 times, for 15 days. This research was designed using a 3x4 Factorial Completely Randomized Design, analyzed using ANOVA and Duncan's Test. The results obtained were that 8 mL/200G BW mustard juice could increase hemoglobin levels and did not increase uric acid levels.
Indonesia is rich in various kinds of traditional fermented foods that can be developed into functional foods, one of which is tape hanjeli. Tape hanjeli is a traditional food typical of the Sukabumi region, West Java, made from hanjeli seeds (Coix lacyma jobi-L) which are fermented using tape yeast. The research aims to test the proximate content and effectiveness of hanjeli tape as an antibacterial for Staphylococcus aureus which causes skin infections. Research methods include making hanjeli tape, testing the proximate content and testing the effectiveness of hanjeli tape as an antibacterial for Staphylococcus aureus (Kirby-bauer). Data analysis was carried out qualitatively and quantitatively. From the results of the proximate content test, the ash content was 0.18%, protein 8.37%, fat 7.85%, carbohydrates 22.45%. The effectiveness test results obtained inhibition zones at concentrations of 60% (1.02 mm), 70% (3.56 mm), 80% (3.06 mm) 90% (5.68 mm). The conclusion of the research stated that hanjeli tape can act as a functional food and is effective as an antibacterial for Staphylococcus aureus which causes skin disease at a concentration of 90% of 5.68 mm and is included in the moderate inhibition zone category.
