In this paper,an electrochemical immunosensor for the rapid detection of ochratoxin A(OTA)was constructed by grafting OTA antibody and diazonium salt on the electrode surface.In this work,the screen-printed carbon electrodes(SPCE)were modified with diazo salt by electro-deposition.The grafted diazo film served as the bottom layer to expose the carboxyl functional groups to the electrode surface.Then carbodiimide was used to activate the carboxyl groups.The antibody and carboxyl groups were crosslinked to conduct amide bonding to realize the immobilization of the antibody.The electrochemical immunosensors were characterized by cyclic voltammetry(CV)and electrochemical impedance spectroscopy(EIS).It was found that under the best working conditions,the linear detection range of the developed electrochemical immunosensor was 20-200 ng/mL(R2=0.997 0),and the detection limit was 0.5 ng/mL.Showing excellent electrochemical perfor-mance in the detection of OTA in labeled samples,the immunosnesor was suitable for the detection of OTA in food.In addition,in the presence of interferent,the sensor was highly selective to OTA and stayed stable for 14 days.The electrochemical immunosensor is easy to construct,sensitive,fast,and stable,with low detec-tion limits,analysis time,and cost for OTA in real samples.
从农家自然发酵黏豆包谷物发酵液中分离乳酸菌,对菌株进行鉴定,并对益生性能进行研究.利用梯度稀释法从黏豆包中筛选获得 6株乳酸菌,经形态学分析并结合 16S rRNA序列分析分别为棒状乳杆菌(Lactobacillus coryni-formis)、植物乳杆菌(Lactobacillus plantarum)、短杆菌(Levilactobacillus brevis)、发酵乳杆菌(Lactobacillus fermentans)、清酒乳杆菌(Latilactobacillus sakei)和曲氏乳杆菌(Lactobacillus curvatus).对菌株的益生性能评价结果表明:菌株HSDF3(曲氏乳杆菌)的抗逆性和耐酸性能较好,pH3.0 的环境中存活率为 100%;耐盐测试中,菌株HSDF9(清酒乳杆菌)的耐盐效果最好,在盐含量 10%的MRS肉汤中仍可存活,活菌数可达 8.93×106 CFU/mL;耐胆盐测试中,菌株HS-DF12(棒状乳杆菌)存活率最高,在 0.3%胆盐浓度的MRS肉汤培养基中培养 3h之后的存活率为 7.171%;耐人工胃液测试中,经过 3h的模拟胃液培养之后,菌株HSDF3(曲氏乳杆菌)的存活率最高,达到 82.91%;耐人工肠液测试中,经过 6h的模拟肠液培养之后,HSDF12(棒状乳杆菌)存活率最高,达到 93.42%;抗生素敏感性试验中,菌株HS-DF15(短杆菌)对四环素、羧苄西林、头孢氨苄、米诺环素、头孢唑啉、氨苄西林、头孢拉定等抗生素均敏感.
随着社会进入大众创业、万众创新的时代,高校纷纷开设了创新创业的相关课程,将创新创业教育融入教学活动之中.随着国家提出进行课程思政改革,高校应进一步推进课程思政与创新创业教育深度融合,探索课程思政在创新创业教育中的应用实践路径,全面提升高校人才培育质量和水平,为社会经济发展提供更为优秀的人才资源.
当归经过不同的加工会对其化学成分产生不同程度的影响.因此,就近 20年来不同加工方式对当归化学成分的含量变化进行文献综述,并对当归加工过程中化学成分变化规律进行探讨,最后对当归的功能性食品及产品的开发进行展望.该文对当归药食价值的深入挖掘、品质提升、工艺创新、新产品开发等具有一定的参考价值.
该研究选用 0.2%腺嘌呤高蛋白饮食诱导的慢性肾衰竭小鼠模型,探究狭鳕鱼蛋白肽(protein peptides of pol-lock,THP)对慢性肾衰竭的改善作用.结果表明,口服 400 μg/mL THP可以有效缓解腺嘌呤诱导的慢性肾衰竭小鼠的肾脏损伤.THP可以改善小鼠的生长状态,促进小鼠饮食,增加小鼠体质量.通过对血清尿素氮和肌酐含量测定发现,THP显著降低尿素氮和肌酐含量(P＜0.01),改善肾脏功能.THP还能明显减轻管腔扩张和肾间质炎性浸润的症状,下调促炎因子肿瘤坏死因子-α、白细胞介素-6、白细胞介素-1β和白细胞介素-18 的mRNA相对水平;同时减轻肾脏细胞外基质过度生产和纤维化,下调人Ⅰ型胶原蛋白和纤维连接蛋白-1的mRNA相对水平.综上,THP能有效改善慢性肾衰竭小鼠的肾脏损伤状态,表明其具有保护肾脏的功效.
