牙龈瘤是一种常见的牙龈增生性疾病,通常发生在龈乳头. 如瘤体组织较大,切除后可能会出现角化龈变窄以及骨面和根面暴露等不良预后情况. 牙龈瘤是一种在牙龈局部生长的炎性反应性瘤样增生物,常发生在牙龈乳头处[1] . 主要病因为菌斑、牙石等外界刺激.
Objective:To explore the mechanism of SIRT7 gene on autophagy protein and macrophage polarization in oral squamous cell carcinoma cells based on the NF-κB signaling pathway.Methods:Human oral squamous cell carcinoma cell line SCC25 cells were divided into SCC25 group(SCC25 cells without intervention),negative control group(SCC25 cells transfected with empty vector pLKO.1-vector-GFP),SIRT7 shRNA group(SCC25 cells transfected with SIRT7 shRNA),SIRT7 group(SCC25 cells transfected SIRT7 lentivirus),combination group A(SCC25 cells +50 μmol/L NF-κB inhibitor PDTC+ SIRT7 shRNA)and combination group B(SCC25 cells +50 μmol/L NF-κB inhibitor PDTC+SIRT7 lentivirus),the relative expression of SIRT7 was detected by qRT-PCR.The number of invasion was detected by Transwell method.The scratch heal-ing rate was detected by scratch test.Immunofluorescence detection of P62,LC3-Ⅱ,the levels of M1 and M2 marker proteins,NF-κB and NF-κB p65 proteins were detected by Western blot.Results:There was no significant difference in SCC25 cells invasion number,scratch healing rate,P62,LC3-Ⅱ,CD163,CD11C,NF-κB and NF-κB p65 expression between SCC25 group and negative control group(P>0.05).The number of cell invasion,scratch healing rate,CD163,NF-κB and NF-κB p65 were increased and CD11C was decreased in SIRT7 shRNA group(P<0.05).The invasion number,scratch healing rate,CD163,NF-κB and NF-κB p65 of SCC25 cells in SIRT7 group were decreased,while CD11C was increased(P<0.05).Compared with combination group A,the invasion number,scratch healing rate,CD163,NF-κB and NF-κB p65 of SCC25 cells in com-bination group B were decreased,and CD11C was increased(P<0.05).Conclusion:Up-regulation of SIRT7 can inhibit the invasion and migration of oral squamous cell cancer cells,reduce the activities of autophagy protein P62 and LC3-Ⅱ,and pro-mote the polarization of macrophage M2 to M1.The mechanism may be related to the regulation of NF-κB by SIRT7.
铁死亡是区别于凋亡、坏死、焦亡等细胞死亡方式的胞内铁依赖程序性细胞死亡方式,研究显示其参与神经系统、心血管系统、消化系统、免疫系统等相关疾病的发生、发展.近年来,谷胱甘肽超氧化物酶-谷胱甘肽-(半)胱氨酸[GPX4-GSH-Cyst(e)ine]途径介导的铁死亡在口腔疾病中也有报道.本文就铁死亡在口腔颌面部疾病中的研究进展及其作用机制作一综述,以期为铁死亡相关口腔疾病的防治提供新思路.
Objective:To analyse the differences in quality of life between patients with genuine halitosis and pseud-ohalitosis and healthy people.Methods:In this case-control study,178 subjects were selected and divided into 64 cases in the genuine halitosis group,60 cases in the pseudohalitosis group and 54 cases in the control group according to the halitosis com-plaints and detection results.A quality of life survey was carried out on these three groups by the 36-Item Short Form Health Survey(SF-36).Results:The genuine halitosis group and pseudohalitosis group scored lower than the control group in the three dimensions of physical functioning,validity and mental health(all P<0.05).Conclusion:Patients with halitosis experi-ence a significant reduction in quality of life in terms of validity and mental health compared to the normal population.
当受损的牙齿需要拔除时,意向性再植术是临床医生在日常实践中的重要治疗选择之一.其中,根尖的良好密封以抵御外界微生物是至关重要的.生物陶瓷材料因其优良的密封性和生物相容性被作为适宜的根尖倒充填材料,有利于提高意向性再植术的成功率.本文就生物陶瓷材料在意向性再植术中的应用现状及相关研究进展进行综述.
