Objective:To explore the effect of membrane potential on osteogenic differentiation of human dental follicle stem cells(hDFCs)and its electrophysiological mechanisms.Methods:hDFCs were isolated and cultured from human dental follicle tissue by enzymatic digestion methods.To evaluate the effects of Kir2.1 channels on the osteogenic differentiation capacity and membrane potentials of hDFCs,osteogenic differentiation induction,Alizarin red staining,real-time reverse transcriptase-polymerase chain reaction(RT-qPCR),and whole cell patch clamp were conducted by using lentiviral particles carrying human Kir2.1 channels specific short hairpin RNA(shRNA)and Kir2.1 channels specific inhibitor(ML133).Ca2+imaging was performed to identify the store-operated Ca2+chan-nels(SOCs)mediated Ca2+influx in hDFCs by using La3+(a SOCs inhibitor)and Thapsigargin(an endoplasmic reticulum Ca2+pump inhibitor),and to evaluate the effect of membrane potential hyperpolarization caused by decreased extracellular potassium level(1 mmol/L)on intracellular Ca2+concentration.Results:Alizarin red staining,RT-qPCR,and patch clamp results showed that blocking the function of Kir2.1 channels or inhibition of Kir2.1 channels expression could inhibit the osteogenic differentiation ability of hDFCs and reverse the membrane potential hyperpolarization associated with osteogenic differentiation.Ca2+imaging results showed that:(1)SOCs channels mediated Ca2+influx in hDFCs.(2)Promotion of membrane hyperpolarization increased the intracellular Ca2+levels in hDFCs,which could be inhibited by blocking the function of Kir2.1 channels or inhibition of Kir2.1 channels expression;by removal of extracellular Ca2+;or by blocking the function of SOCs channels.Conclusion:Kir2.1-linked membrane hyperpolarization controls osteogenic differentiation of hDFCs by increasing intracellular Ca2+levels and SOCs channels-mediated Ca2+influx plays an important role in this process.
To investigate the clinical application of fiberoptic endoscopic evaluation of swallowing (FEES) and recovery of swallowing function in patients with lesions involving both the oral and base of the tongue (OBOT). Patients diagnosed with OBOT lesions underwent assessments preoperatively and at 1 week, 1 month, and 1 year postoperatively. The swallowing function evaluation included the MD Anderson Dysphagia Inventory (MDADI), University of Washington Quality-of-Life Questionnaire, Performance Status Scale for Head and Neck Cancer, water swallow test, tongue motion measurement, and FEES (a simple dysphagia score, and penetration-aspiration scale). Postoperative swallowing rehabilitation uses functional therapy. In total, 18 patients participated in this study. The FEES-based dysphagia score had high reliability and diagnostic value. FEES parameters were significantly correlated with results of the water swallow test and MDADI scores. At 1 year postoperatively, the swallowing function of patients with OBOT lesions returned to preoperative levels, although their quality of life was impacted. FEES is a reliable method for evaluating the postoperative swallowing function of patients with OBOT lesions and is worth clinically promoting. Although the swallowing function of these patients was restored, attention should be paid to the quality of life.
目的:探讨改良切口在上颌中低位埋伏阻生牙拔除术中的效果.方法:选取于2020年3月～2022年3月在我院口腔颌面外科门诊就诊的上颌中低位埋伏阻生牙患者50例.按随机数字表法将该群体随机平均分成实验组和对照组,每组各25例.实验组采用改良切口翻瓣术,对照组采用传统角形瓣翻瓣术.比较两种拔牙翻瓣术式切口缝合操作时间、肿胀情况、创口愈合情况、开口度、拆线操作时间的差异,运用简化McGill疼痛问卷评估两组患者疼痛差异,采用SPSS 21.0软件包对数据进行统计学分析.结果:实验组切口缝合、拆线操作时间均明显少于对照组(P＜0.05).实验组术后2天疼痛明显轻于对照组(P＜0.05).但实验组与对照组的肿胀、创口愈合、开口度情况比较无统计学差异(P＞0.05).结论:改良切口翻瓣术使缝合、拆线操作时间较传统角形瓣缩短,且缓解了术后疼痛反应,提高患者舒适度,值得推荐使用.
在树脂粘接修复的过程中,牙釉质和牙本质的表面处理是关键步骤.目前,传统酸蚀剂常用的磷酸是一种强酸,容易对牙本质造成侵蚀性脱矿,影响树脂-牙本质粘接界面的长期稳定性.近年来,有研究将乙醇酸作为一种替代酸蚀剂,本文将介绍乙醇酸的应用背景和酸蚀原理,重点针对使用乙醇酸酸蚀牙釉质和牙本质的酸蚀效果及与传统磷酸酸蚀相比的优势做一综述,以期为后续的临床研究提供参考.
近年来,人工智能在口腔正畸学领域应用的研究日渐增多,多种人工智能模型被证实可用于正畸诊断、治疗决策、疗效预测等方面.研究表明,人工智能在正畸中的应用能够提高临床诊疗效率,具有较高的准确性,但也存在数据量不足、样本来源单一、人工智能模型可解释性差等问题.本文拟通过回顾人工智能在正畸诊断、治疗决策和疗效预测三方面的研究现状,以期对人工智能在口腔正畸领域的应用有较全面的认识,为相关研究和临床应用提供参考.
