The chicken carcass is a widely consumed and affordable food item known for its nutritional richness. However, susceptibility to spoilage due to microorganisms necessitates the use of natural preservatives. Saccharomyces cerevisiae yeast, capable of producing organic acids that limit microbial growth, is employed. This research aims to determine the shelf life of chicken carcasses with varying percentages of Saccharomyces cerevisiae, assessed through total bacterial colonies, initial decay, and organoleptic properties (color, aroma, texture, taste, and overall acceptability). Conducted in October 2022 at the Laboratory of Livestock Product Processing Technology, Universitas Padjadjaran, the study utilized the Randomized Completely Block Design (RCBD) with treatments P0 (0%), P1 (1%), P2 (2%), P3 (3%), and five replications. Results indicated no significant differences, with P2 demonstrating the best outcomes for total bacteria and initial decay. P2 exhibited the best color, a pale white hue, while P1 had a distinctive meat aroma. P1 displayed the best texture, characterized by tenderness, and the best taste, slightly bland. P0 (0%) achieved the highest overall acceptability. Statistical analysis concluded that the addition of Saccharomyces cerevisiae had no significant impact on total bacterial colonies, initial decay, and organoleptic properties in chicken carcasses.
The chicken farming industry plays an important role in the Indonesian economy. Barriers are often felt by chicken farmers, such as the threat of disease, oneof which is caused by ectoparasite attacks. The purpose of this study was to determine the type and the level of diversity of ectoparasites, and to determine the effect of environmental conditions on the level of distribution of ectoparasites on chicken farms in Karangreja and Kemangkon. This research was conducted in two chicken farms located in Karangreja and Kemangkon sub-districts, Purbalingga, Central Java starting from January to March 2021. The methods used in this study were observing the general condition of the location, taking ectoparasites in chicken feces, sample preparation, and specimen identification. Data analysis was carried out by determining the level of density, relative density, frequency, relative frequency, importance of ectoparasites, and knowing the Shannon-Wiener diversity index of ectoparasites in chickens. The results showed that the ectoparasites found in laying hens in Karangreja were Urodiaspis sp., Tyrophagussp., Urodiaspis pannonica, Uroobovella sp., Androlaelapssp., and Beierochelifer peloponnesiacus. Ectoparasites that can be found in Kemangkon are Urodiaspissp., Tyrophagussp., Macrocheles mammifer, Macrocheles muscadomesticae, and Beierochelifer peloponnesiacus. The level of diversity of ectoparasites in laying hens inKarangreja is 1,023 and is classified as moderate diversity. The level of diversity of ectoparasites in laying hens in Kemangkon is 0.710 and is classified as low diversity. Environmental conditions that affect the diversity of ectoparasites are temperature, soil pH, air humidity, and soil moisture.
Beltsville Thawing Solution (BTS) is a common diluent used in the preservation of pig sperm. However,its capacity to preserve sperm motility and longevity, is severely limited. One of the reasons is the diluent lacks antioxidants in sufficient amounts to counteract the harmful effects of free radicals. The objective of the study wasaimed to evaluate the efficaciousness of BTS,Mulberry III (MIII)and Sperm Life diluents in conjunction with Moringa leaf ethanol extract (MLe2) for the preservation of landrace pigs’sperm.Semen was collected from one landrace boar aged 2.9years. Semen evaluation was performed both macroscopically and microscopically, and good quality semen was split into three tubes and diluted with BTS, MIII and Sperm Life diluent, to which 4% MLe2 had been added. Semen preservation was done in a cool box at a temperature of 18-20oC andevery eight hours, sperm motility, viability, intact-plasma membrane and acrosomal were assessed. Data were analyzed using analysis of variance and continued with the Duncan Test.The study's findings demonstrated that, in comparison to Sperm Life, BTS and MIII diluents added with MLe2 produced sperm of higher quality. It was concluded that BTS and MIII diluents enriched with E2dk were more effective than Sperm Life in maintaining the sperm quality of landrace pigs
Antibiotic resistance was a global threat in the health sector and could arise from various sectors. The application of integrated farming systems, including the integration of dairy farming with agriculture, could potentially increase the risk of spreading resistance. This study was aimed to determine the antibiotic resistance profile of Escherichiacoliisolated from fresh feces of dairy cows, feces from manure pits, soils, plants, clean water, wastewater, and milk from two agricultural-integrated dairy farms in Jayagiri Village, Lembang District, Bandung Barat Residence of Jawa Barat Province.Isolation and identification of E. coliwas carried out with Eosin Methylene Blue(EMB)media, Gram stain, biochemical tests and antibiotic susceptibility test using the Kirby-Bauer disk diffusion method. Antibiotic susceptibility test showed resistance of 20% E. coliisolates from fresh fecal origin to ampicillin, intermediate resistance of 17% E. coliisolates from fecal in manure pits origin toampicillin, and resistance of 33% E. coliisolates from clean water origin against tetracycline. Meanwhile, ceftriaxone, gentamycin, and ciprofloxacin remained 100% sensitive in all types of samples. Eschericiacolibacteria and resistance found in this study indicated that integrated farming system could be a source of E. coliand antibiotic resistance contamination
Various animal diseases require colorimetric-based laboratory diagnose. One of them is the measurement of blood hemoglobin concentration. Anemia needs to be detected as early as possible, because the lack of hemoglobin inthe blood could results in a lack of oxygen being carried from the lungs to the tissues. Colorimetry is a quantitative analysis technique for colored samples to determine the concentration of a substance based on the light intensity of the color solution. Colorimetry is commonly used to measure the level of hemoglobin in the blood. Image processing using imageJ has been widely used for chemical analysis. This study was aimed to determine the potential of image processing from a desktop scanner to measure animal blood hemoglobin. The blood used was taken from five quail. Quail blood was diluted to 100%, 90%, 80%, 70%, 60% and 50% respectively. The results of hemoglobin level readings using the Sahli method were strongly correlated, but significantly different, with a spectrophotometer. The readings of hemoglobin levels using a desktop scanner have a very strong correlation and are not significantly different from the results obtained using a spectrophotometer. The image processing method from a desktop scanner can be used as an alternative to a spectrophotometer to measure blood hemoglobin levels compared to using the Sahli method.
