Drug–target affinity (DTA) prediction is a critical step in virtual screening and significantly accelerates drug development. However, existing deep learning-based methods relying on single-modal representations (e.g., text or graphs) struggle to fully capture the complex interactions between drugs and targets. This study proposes CM-DTA, a cross-modal feature fusion model that integrates drug textual representations and molecular graphs with target protein amino acid sequences and structural graphs, enhancing feature diversity and expressiveness. The model employs the multi-perceptive neighborhood self-attention aggregation strategy to capture first- and second-order neighborhood information, overcoming limitations in graph isomorphism networks (GIN) for structural representation. The experimental results on the Davis and KIBA datasets show that CM-DTA significantly improves the performance of drug–target affinity prediction, achieving higher accuracy and better prediction metrics compared to state-of-the-art (SOTA) models.
随着全球环境污染加剧、气候持续变化和人口不断增长,如何保障安全、营养和可持续的食品供给面临巨大挑战.这些挑战对未来食品供给方式和功能提出了新的要求.采用合成生物学技术,创建适用于食品工业的细胞工厂,将可再生原料转化为重要食品组分、功能性食品添加剂和营养化学品是解决食品领域所面临问题的重要途径.本文首先介绍了合成生物学对食品制造领域创新和突破的重要性.其次,以基于合成生物学制造植物蛋白肉所需关键组分、黄酮类植物天然提取物和母乳寡糖这三种典型食品为例,探讨了目前食品合成生物学的任务与挑战.最后,对我国合成生物学与食品制造领域的发展趋势进行了总结和展望.通过加强食品合成生物学等具有重大意义的食品生物技术的开发和应用,开展新食品资源开发和高值利用、多样化食品生产方式变革、功能性食品添加剂和营养化学品制造,并率先实现产业化,将抢占世界科技的前沿和产业高地,造福人类.
The poor gel properties of fish gelatin limit its commercial application.Enzyme-catalyzed modification of fish gelatin has great advantages such as environmental friendliness,safety and high efficiency.However,there are few gelatin-modified enzymes reported at present,and most of them are covalent cross-linking enzymes,which easily make gelatin form thermal irreversible gel.In this study,two proline hydroxylases that increase the non-covalent action of collagen were cloned and expressed in prokaryotic cells.After purification,the fish gelatin was catalyzed respectively using two proline hydroxylases.The results show that the two enzymes have the effect of improving the gel strength and texture characteristics of fish gelatin.In addition,the surface display technology of Corynebacterium crenatum was studied.The two enzymes were displayed on the surface of C.crenatum,and an immobilized enzyme system with C.crenatum as the carrier was prepared for studying the modification effect of two immobilized enzyme systems on fish gelatin.This study has enriched the catalytic enzyme system of fish gel and provided a new idea for the catalytic modification of fish gelatin.
Objective:To prepare anti-ET antibody for rapid detection of ET using the colloidal gold method.Methods:After identified by mass spectrometry and ultraviolet spectrum,the synthetic ET antigen was used to immunize Balb/C mice.ET hybridoma cell lines with high specificity were obtained by hybridoma technique.After purification by Protein G column,the antibody with appropriate sensitivity was selected and applied to ET colloidal gold reagent.The sensitivity and specificity of the antibody were identified by drug cross interference experiment and clinical experiment.Results:The antibody produced by 10F9 cell line was suitable for ET colloidal gold immunochromatography reagent.The ET reagent was used to detect 12 kinds of e-liquid additives and 48 kinds of commonly used drugs without cross-reaction.The cutoff of ET reagent in urine and e-liquid were both 500 ng/mL,the sensitivity was 100％,and the specificity was 99.5％.Conclusions:The monoclonal antibody(mAb)against ET was successfully prepared and applied to the colloidal gold immunochromatographic reagent.
Programmed cell death(PCD)is a collective term for the intrinsically regulated death of cells.Different forms of cell death are caused by their own programmed regulation during the growth and development of organisms and stress responses to the environment and diseases.PCD includes apoptosis,pyroptosis,necroptosis,autophagy and ferroptosis.It is not only essential for the growth and development of organisms,but also plays an important role in intervening in the process of pathogens invasion.Staphylococcus aureus can regulate various forms of PCD such as apoptosis,pyroptosis,necroptosis and autophagy in host cells,thereby affecting bacterial infection.This article reviews the crosstalk between Staphylococcus aureus infection and PCD to further understand the relationship between Staphylococcus aureus and cell death,in order to provide new ideas for the diagnosis and treatment of clinical Staphylococcus aureus infection.
