Objective To observe the effects of 9L/IL-18 on the expressions of CD80,CD86,MHCⅠand MHCⅡin tumour tissues of rats transfected with IL-18, and to explore the anti-tumour effects of IL-18.Methods The F344 rats were randomly divided into three groups ,with 7 rats in each group,and these rats were inoculated with 9L/IL-18 cells,9L/LXSN cells,9L cells,respectively.The expression levels of CD80,CD86,MHCⅠ,MHCⅡin tumor tissues were detected by flow cytometry.Results The expression levels of CD80,CD86,MHCⅠin tumor tissues of rats inoculated with 9L/IL-18 cells were significantly higher than those in tumor tissues of rats inoculated with 9L/LXSN cells and 9L cells ( P <0.05).However there were no significant differences in the expression levels of CD 80,CD86,MHCⅠbetween 9L/LXSN cell inoculation group and 9L cell inoculation group ( P >0.05).There were no significant differences in the expression levels of MHCⅡ among the three groups ( P >0 .05 ) .Conclusion IL-18 may promote the differentiation and maturation of dendritic cells by increasing the expressions of CD80, CD86, MHCⅠon the surface of tumor cells,thereby,which may activate T cells,enhance the antigent activity of tumour cells to improve the effects of immune surveillance and to produce anti -tumor effects .

Objective:To assess the effect on the expression of TGF -βand Fgf2 of the exogenous IL -18 gene on the biological characteristics of rat 9L glioma cells.Methods:The cell suspension of 9L/IL -18,9L/LXSN and 9L cells were inoculated into the brain of F344 rat,detct the level of TGF -βand Fgf2 by enzymelinked immunosorbent assay and RT -PCR.Results:The level of TGF -βin the peripheral blood of rats inoculated 9L/IL -18 was weak-end to that inoculated 9L/LXSN and 9L(P <0.05).Compared to 9L/LXSN and 9L cells inoculated rat,the expression of Fgf2 were decreased in the tumor tissue of rat inoculated 9L/IL -18.Conclusion:IL -18 could decrease the levelof TGF -βand Fgf2.

Objective To analyze the effects of IL-18 transfection on the 9L glioma growth in vitro and the apoptosis of tumor cells.Methods 9L/IL-18,9L/LXSN and 9L cells were inoculated into the brain of F344 rat to establish the animal models by the stereotactic technique.The tumorigenicity of different cells was observed, and then the tumorigenic rates were calculated. The cell apoptosis was detected by TUNEL.The apoptosis rate of tumor cells in each group was calculated.The expression of Bax and Bcl-2 in tumor tissues was detected by RT-PCR and Westen Blot. Results The rate of tumor formation in each groups was 100%;TUNEL assay showed that a large number of apoptotic cells were detected in the tumor tissues of the 9L/IL-18 cell group,in which the apoptotic rate was (22.2±1.02)%,significantly higher than that in 9L/LXSN (5.7±0.32)% and 9L (6.4±0.51)% groups (P<0.05);the expression of Bax in the tumor tissues of inoculated 9L/IL-18 cell group was higher than those of the other groups, and the expression of Bcl-2 was lower than those of the other groups.Conclusion By up regulating the expression of Bax,down regulating the expression of Bcl-2,transfected exogenously IL-18 gene can pro-mote the apoptosis of tumor cells and play an anti-tumor role.

Objective To establish the reference value ranges of specific thyroid function indexes among pregnant women in the Longgang District Maternity and Child Healthcare Hospital for avoiding the misdiagnosis and missed diagnosis of pregnant thyroid diseases .Methods A total of 637 normal pregnant women with establishing the card in the outpatient department of this hospital and 201 non‐pregnant women undergoing the normal physical .The fasting venous blood was collected .The values of FT3 ,FT4 ,T3 , T4 and TSH were detected by adopting the chemiluminescence immunoassay .The median and 95% credibility interval statistical method was used to establish the normal reference value ranges of specific serum free triiodothyronine(FT3) ,free thyroxine(FT4) , three icdine thyroid gland(T3) ,total thyroid hormone(T4) and thyroid stimulating hormone(TSH) in pregnancy .Results The me‐dian(M value) and double‐side limiting value(P2 .5 and P97 .5 ) were used to express the reference value ranges of thyroid hormones in early ,middle and late pregnant women and non‐pregnant women .FT3 and FT4 were significantly increased at early pregnancy ,then gradually increased with the pregnant period progression ,began to decrease from middle pregnancy and were higher than the levels before pregnancy .FT3 and FT4 were slightly increased ,then progressively decreased .TSH was significantly decreased in early pregnancy ,began to increase at middle pregnancy and was higher than the level in non‐pregnant women until late pregnancy .Conclu‐sion The establishment of reference value ranges of specific thyroid hormones during various stages of pregnancy ,which provides the clinical data for the diagnosis and treatment of thyroid disease during pregnant period;the change rule of thyroid hormones in pregnant women provides a theoretical foundation for the prevention of thyroid disease during pregnant period .

Objective To investigate and analyze the clinical application value of bedside rapid combined detec‐tion of cardiac troponin Ⅰ(cTnI) ,creatine kinase(CK‐MB) and myohemoglobin(MB) for the diagnosis of acute myo‐cardial infarction(AMI) .Methods 138 emergency cases with chest pain onset less than 3 h in our hospital from Jan‐uary 2013 to August 2014 were selected as the research subjects and divided into the AMI group (observation group) and non‐AMI group (control group) according to the diagnostic criteria of AMI .The Judkins method was adopted to conduct the coronary arteriongraphy via right femoral artery with 6 F coronary arteriography catheter .The diagnosed AMI patients were treated by adopting percutaneous coronary intervention (PCI) .The timing extraction of venous blood samples was performed for detecting the serum levels of cTnI ,CK‐MB and MB .Results The detection results at various time periods in the two groups showed that serum cTnI ,CK‐MB and MB levels in the observation group were significantly higher than those in the control group (P<0 .05) .The sensitivity and the specificity in the com‐bined detection of serum cTnI ,CK‐MB and MB levels for diagnosing AMI were 97 .2% and 88 .3% respectively , which were significantly higher than 86 .8% ,81 .3% ,84 .2% and 78 .6% ,74 .6% ,72 .5% in the single detection of these 3 indicators respectively ,the differences were statistically significant (P<0 .05) .Conclusion The bedside rapid combined detection of cTnI ,CK‐MB and MB can significantly increase the sensitivity and specificity for diagnosing AMI ,has the results consistent to that of the coronary angiography and can be promoted and applied in clinic .

目的探讨血浆B型钠尿肽（BNP）对肺心病的预后评估意义，为临床诊治工作提供参考依据。方法选择2009年3月2012年3月收治的82例肺心病作为研究对象，并以同期健康者30例作为对照，分别采用快速免疫荧光法测定血浆BNP水平并进行比较分析，对肺心病患者进行治疗并进行跟踪随访，按照预后分为两组，比较不同预后患者的血浆BNP水平。结果肺心病患者的血浆BNP水平明显高于健康者（P〈0.05），且患者的血浆BNP水平随着心功能不全I、II、III、IV级依次递增；预后良好的患者血浆BNP水平明显低于预后不良者（P〈0.05）。结论肺心病患者的血浆BNP水平明显增高，且与疾病的严重程度关系密切，对该疾病的诊断及治疗预后有重要的临床价值。

Objective:To explore the influence of IL-18 on the expression of MHCⅠ,MHCⅡ,CD80,CD86 on the surface of 9L cells,to identify the effect of IL-18 on the immunogenicity and the efficiency of tumor antigen presentation of 9L.Methods:Retroviruses were used to transduce the mIL-18 gene into rat glioma cells and the cell clones (9L/IL-18) which steadily expressing mIL-18 gene were obtained ,and got the control cells of 9L/LXSN by the same method.The expression of MHCⅠ,MHCⅡ,CD80,CD86 on the cell surface were assessed by flow cytometry.The cell suspension of 9L/IL-18,9L/LXSN and 9L cells were inoculated into the brain of F344 rat to establish the animal models by the stereotactic technique ,got the tumor tissues to analyze the expression of MHCⅠ, MHCⅡ,CD80 and CD86 on the surface of tumor cells after 14 days.Results:The expression of MHCⅠ,MHCⅡ,CD80 and CD86 on the surface of 9L/IL-18 were (10.9±1.44)%,(0.61±0.14)%,(1.01±0.14)%,(0.57±0.11)% ; had no significant differences with the others in vitro (P>0.05);while the expression of MHCⅠ,CD80 and CD86 on the surface of 9L/IL-18 were(67.51± 1.40)%,(12.51±1.57)%,(6.95±0.56)%which were higher than 9L/LXSN and 9L cells (P<0.05),the expressions of MHCⅡwere no significant difference(P>0.05) in vivo.Conclusion: IL-18 did not affect the immunogenicity of 9L in vitro,but improve the immunogenicity and tumor antigen presentation in vivo.

Objective To explore the Clinical Significance of BNP for Risk Stratification,Treatment and Prognosis in Submassive PTE. Methods A total of 56 patients with confirmed submassive PTE was divided into two groups according to plasma BNP levels,according the treatment: blood gas analysis,echocardiography and the prognosis were analyzed and compared in ENPpatients. Results Comparing the two groups,the observation group PO2,A-aDO2,oxygenation index,myocardial enzyme in plasma increased incidence of( or),right ventricular and right atrial enlargement,PASP,middle( or weight) of three tricuspid regurgitation rate,recurrence within 1 years,combined with pulmonary artery high pressure,with pulmonary heart disease and death rate compared with the control group,the difference has statistical significance( P<0. 05). Conclusion The detection of plasma BNP contributes to the high-risk screening and prognostic assessment of submassive P1E. and has clinical significance for selectingthe treatment scheme as well.

1IL-18对NK细胞增殖和分化的影响及其机制 在免疫应答中NK细胞的增殖和分化受多种细胞因子的调节[1].例如,IL-2、IL-12、IFN-α、TNF-α以及白细胞调节素(Leukoregulin, LR) 对NK细胞的活化和分化都有正调节作用,体外培养时加入上述细胞因子可明显提高NK细胞的杀伤活性.前列腺素 (PG)E1、E2、D2和肾上腺皮质激素等则对NK细胞的活性有抑制作用.Jason等[1]最近的研究表明IL-18可促进NK细胞的成熟.NK细胞可以通过分泌TNF-α、IFN-γ和细胞与细胞间的接触诱导DC细胞的活化和成熟.在体外,活化的DC细胞所产生的IL-12、IL-12/IL-18、IL-15及IFN-α/β可以诱导NK细胞产生IFN-γ,促进NK细胞的增殖,发挥细胞毒作用[2].

Objective: To investigate the expression of regulatory T lymphocytes in human astrocytoma and its correlation with the progression of astrocytoma. Methods: Data were collected from astrocytoma patients treated in The 4th Hospital Affiliated to Hebei Medical University between September 2008 and June 2010. The expression of CD4+ CD25+ FOXP3+ regulatory T lymphocytes (Treg) in the tumor tissue and blood were detected by flow cytometry (FCM). The expression of transforming growth factor β1 (TGFβ1) was detected by ELISA test. Data were analyzed by SPSS software 13.0. Results: A total of 92 patients (54 males and 38 females, with a mean age of 51.4 years) who underwent surgical treatment were divided into three groups according to the pathological grade (42 grade IV cases, 21 grade III cases, 29 grade II cases. The controled brain tissue was obtained from 8 patients who underwent a temporal lobectomy for seizures. There were significant differences in Treg cells between astrocytoma patients and the controls (10.67 % ± 0.12 %) both in the brain tissue (24.17 % ± 2.54 %) and in the blood (9.34 % ± 2.13 %) (P< 0.01). The expression of Treg in astrocytoma was positively correlated with the tumor grade (P< 0.05). The expression of TGF β1 in the blood of glioma patients was positively correlated with Treg cells (r = 0.564, P= 0.001). Conclusion: Our study showed significant increase of Treg cells in the tumor tissue and peripheral blood of patients with astrocytoma. The role of Tregs in the tumor biology of gliomas might have potential therapeutic implications.