Objective Myocardial infarction (MI) is a common and serious cardiovascular disease with increasing incidence and mortality rates, making it a major global public health issue. Molecular biology research has shown that the cleavage products miR-208 and miR-92a are microRNAs (miRNAs) associated with myocardial injury. Therefore, this study aims to establish a predictive model and explore the application value of the combined detection of miR-208 and miR-92a in the early diagnosis of MI in microRNA. Methods Plasma samples were collected from 231 volunteers divided into 30 healthy and 201 diseased subjects From January 1st, 2021 to December 30th, 2021. Plasma RNA was extracted using a TRIZOL kit, and levels of miR-208 and miR-92a were determined using a real-time polymerase chain reaction (PCR) assay. Subsequently, the logistic regression model, decision tree model analysis, and receiver operating characteristic (ROC) curve were used to evaluate whether miR-208 combined with miR-92a could be used as a biomarker for MI early diagnosis. Results In this study, the ROC curve evaluation of the logistic regression model and pruned decision tree model found that age, miR-208, and miR-92a had high early diagnostic accuracy for MI, and the area under the curve (AUC) reached 0.928, showing good predictive value. It was also found that the AUC, optimal threshold, sensitivity, and specificity of age, miR-208, and miR-92a were higher than those of age and miR-208. This indicates that the combination of age, miR-208, and miR-92a has more value in the early diagnosis of MI. Conclusion The combined diagnosis of miR-208 and miR-92a is helpful for the early diagnosis of myocardial infarction, which might serve as a new marker of MI benefiting from its early diagnosis.

Fractures are frequently encountered diseases troubling the senior population, and the research on fracture repair and the exploration of effective treatment methods are of great significance. This study aimed to clarify the effect of human umbilical cord mesenchymal stromal cell-derived extracellular vesicles (hUMSC-EVs) on the proliferation and osteogenic differentiation of autologous bone marrow stem cells (ABMSCs). The two kinds of cells were co-cultured firstly, 5-Ethynyl-2’- deoxyuridine (EDU) staining and alizarin red staining were used to detect the proliferation and osteogenic differentiation of ABMSCs. The exosomes of hUMSCs were subsequently extracted to process ABMSCs to further test the effect on the cells. The EDU positive rate of ABMSCs and Collagen II expression were elevated, whereas the TdT-mediated dUTP nick end labeling (TUNEL) positive rate and Matrix Metallopeptidase 13 (MMP13) were markedly decreased after the co-culture of hUMSCs and ABMSCs using Transwell chamber assays. The results indicated that hUMSCs could increase the proliferation of ABMSCs, reduce apoptosis, and promote matrix metabolism. The hUMSCs exosomes were separated and added to ABMSCs. As the exosomes content increased, the proliferation of ABMSCs increased simultaneously, and ABMSCs apoptosis decreased. Meanwhile, ABMSCs that migrated to the submembrane increased compared with untreated ABMSCs. Western blot, qPCR and immunofluorescence results revealed that increased exosomes contents promoted the expression of ABMSCs anabolic-related indicators gradually, while decreased the expression of catabolism-related indicators gradually. The previously described results indicated that hUMSCs promoted the proliferation and osteogenic differentiation of ABMSCs by secreting exosomes.

MutS homolog 2 (MSH2) is a crucial participant in human DNA repair, and lots of the studies functionally associated with it were begun with hereditary nonpolyposis colorectal cancer (HNPCC). MSH2 has also been reported to take part in the progresses of various tumors' formation. With the help of GTEx, CCLE, and TCGA pan-cancer databases, the analysis of MSH2 gene distribution in both tumor tissues and normal control tissues was carried out. Kaplan-Meyer survival plots and COX regression analysis were conducted for the assessment into the MSH2's impact on tumor patients' clinical prognosis. In an investigation to the association of MSH2 expression with immune infiltration level of various tumors and a similar study on tumor immune neoantigens, microsatellite instability was subsequently taken. It was found that high expression of MSH2 is prevalent in most cancers. MSH2's efficacy on clinical prognosis as well as immune infiltration in tumor patients revealed a fact that expression of MSH2 in prostate adenocarcinoma (PRAD), brain lower-grade glioma (LGG), breast-invasive carcinoma (BRCA), and head and neck squamous cell carcinoma (HNSC) posed a significant correlation with the immune cell infiltration level of patients. Likewise as above, MSH2's expression comes in a similar trend with tumor immune neoantigens and microsatellite instability. MSH2's expression in the majority of tumors is a direct factor to the activation of tumor-associated pathways as well as immune-associated pathways. MSH2's early screening or even therapeutic target role for sarcoma (SARC) diagnosis is contributing to the efficiency of early screening and overall survival in SARC patients.

临床微生物学检验是一门实验室科学与临床医学相结合的学科,临床实习阶段是深化理论知识、提升专业实践能力的关键阶段.带教教师拥有不断更新的教学理念,设计出切实可行的带教流程,是实习带教的核心.该文从实验室安全培训、专业知识培训与考核、科研创新等方面阐述了临床微生物带教方案的实施过程,从而达到提升实习生专业实践能力和综合思维能力的目的.

目的 探讨利用PDCA循环管理模式优化血培养报告流程对脓毒症诊断能力的提升作用.方法 利用鱼骨图分析影响阳性血培养报告临床实验室标本周转时间(TAT)的因素,针对影响因素提出整改措施,对阳性血培养的流程进行优化,并将处理流程分解为6个阶段,分析项目启动前后阳性血培养处理流程中各阶段TAT.收集了该院检验科微生物组105例患者的阳性血培养报告.改进前(2020年1-3月)41例,其中肠杆菌科细菌25例;改进后(2020年4-6月)64例,其中肠杆菌科细菌51例.采用SPSS24.0统计软件进行非参数检验.结果 与实施前比较,项目实施后阳性血培养报告TAT中位数缩短了19.2 h(P<0.01),血培养阳性的肠杆菌科细菌报告TAT中位数缩短了18.6 h(P<0.01),完成了项目的预定目标.结论 通过PDCA循环法管理工具,分析了影响阳性血培养报告TAT的关键因素,建立数据收集策略,并且加以改正,切实提高了实验室阳性血培养的处理能力,达到提升脓毒症诊断能力的目的.

目的:比较微创前外侧入路(orthop dische chirurgie München,OCM)与常规后外侧入路(posterolateral approach,PLA)在初次全髋关节置换中的早期临床疗效.方法:分析2014年8月至2016年10月重庆市第四人民医院收治的82例行初次全髋关节置换术患者,根据入路方式不同随机分为OCM组与PLA组,分别对2组的切口长度、手术时间、手术总出血量、输血率、术后开始下床时间、术前与术后髋关节Harris评分、疼痛视觉模拟评分(visual analogue scores,VAS)、术后放射学检查假体位置等进行对比评估.结果:2组在切口长度,手术时间,手术总出血量,术后开始下床时间,术后24 h、48 h、72 h VAS及术后1周、1个月、3个月Harris评分比较,差异均有统计学差异(P＜0.05);而2组输血率,术后1周VAS,术后6个月、12个月、24个月髋关节Harris评分及假体位置无统计学差异(P＜0.05).2组患者均无脱位情况发生,OCM组患者1例(0.71％)术中发生股骨距骨折,经钢丝环扎固定处理,随访过程显示假体稳定,无松动、下沉.结论:与PLA入路比,OCM入路初次全髋关节置换切口小,出血少,早期临床效果更佳.

To investigate the drug release and anti-adhesion effects of a TET (tetrandrine)-loaded PDLLA (poly-dl-lactide) film. Detection of TET release in vitro was carried out by high-performance liquid chromatography (HPLC) every 2 days following immersion of the tetrandrine-loaded PDLLA film in simulated body fluid until the TET content of the eluate could not be detected. For the in vivo test, TET-loaded PDLLA films were implanted into animal laminectomy models and positive and blank control groups were also set up. Postoperative serum tests, and macroscopic and histological analyses at 1, 4, 8, and 12 weeks, were used to assess the effects of the film. Statistical analyses were performed by one-way ANOVA. The drug release of the tetrandrine-loaded PDLLA film in vitro showed two phases with a second release peak. Ultimately, the duration of continuous delivery was up to 66 days and the cumulative delivery rate was up to 93.18%. Scores for the proliferation of epidural scars or adhesion of the dura mater in the test group were much lower than those for the two control groups. Histological analysis revealed the test group had fewer inflammatory cells and fibroblasts, as well as fewer extracellular collagen fibers, and a lower histology score than those of the two control groups at all time points. Tetrandrine-loaded PDLLA film is a novel controlled drug release and anti-adhesion material in vitro and in vivo.

Objective The present study investigated the effects of pomegranate ethyl acetate extract (PEE) on cell viability of primary rat islet cells.Methods PEE was extracted by using 95％ ethanol and a series of organic solvents.Primary isolated islet cells of rat were cultured in high glucose and/or high fat conditions with or without PEE.Cell viability was determined by MTT assay.Insulin secretion was assessed by using radioimmunoassay.Results Insulin levels were significantly higher in cells treated with high glucose+PEE,palmitic acid+PEE,and olein acid+PEE compared to the cells treated with high glucose,palmitic acid,or oleic acid alone,in which the insulin levels were markedly less than that of the normal control group.Conclusion PEE promotes islet cell growth and insulin secretion,which is suggested that PEE can protect islet cell function in both high glucose and high fatty acid environments.

随着社会发展和科学技术进步,人类社会对能源的需求越来越大.陆上油气资源经过长时期大规模的开采,变得日益贫乏,世界范围的油气勘探与开发转向了资源丰富的、占地球表面71%左右的辽阔海洋.海洋油气资源开发逐渐成了能源工业中投资高、风险大、高新技术密集的新领域.而大型全回转起重船是典型的大型海洋装备,其在石油工程、港口工程、桥梁工程、打捞工程以及大型海上军事装备工程等领域都有着广泛应用,拥有举足轻重的地位.

OBJECTIVETo construct and identify recombinant adenovirus Ad-IRF8 carrying mouse interferon regulatory factor-8 (IRF8) and observe the influence of the over-expressed IRF8 on the differentiation of osteoclast precursor cells (RAW264.7) induced by soluble receptor activator of nuclear factor κB ligand (sRANKL).METHODSMouse IRF8 gene amplified by PCR was subcloned into the shuttle vector pAdTrack-CMV. The vector pAdTrack-IRF8-CMV was linearized by Pme I , followed by homologous recombination with bone vector pAdEasy-1 in E.coli BJ5183. Recombinant adenovirus vector pAd-IRF8 was obtained and linearized by Pac I , and then transfected into AD293 cells. The recombinant adenovirus Ad-IRF8 was harvested after packaged in AD293 cells. RAW264.7 cells were infected by Ad-IRF8. The overexpression of IRF8 was verified with semi-quantitative reverse transcription-PCR (RT-PCR) and Western blotting. The influence of the overexpression of IRF8 on sRANKL-induced differentiation of RAW264.7 cells was detected by tartrate-resistant acid phosphatase (TRAP) staining.RESULTSThe shuttle vector pAdTrack-IRF8-CMV and recombinant vector pAd-IRF8 were successfully constructed. The recombinant adenovirus Ad-IRF8 was also successfully packaged in AD293 cells. The overexpression of IRF8 in RAW264.7 cells was confirmed by RT-PCR and Western blotting. TRAP staining showed that the overexpression of IRF8 effectively inhibited the differentiation of RAW264.7 cells into osteoclast-like cells.CONCLUSIONThe recombinant adenovirus Ad-IRF8 was successfully constructed and the overexpression of IRF8 was also detected in RAW264.7 cells. The overexpressed IRF8 can effectively inhibit the differentiation of RAW264.7 cells into osteoclast-like cells.

Objective A mouse model of early postoperative implant infection was developed to compare the serum level of interleukin-6(IL-6) and C-reactive protein(C-RP).Methods Forty female adult Balb/c mice were equally divided into A,B,C and D group at random.After locating the femoral trochlear groove,the right femoral intramedullary canal was manually reamed,an aseptic stainless syringe needle was surgically placed in a retrograde fashion into the intramedullary canal of the right femur of group C and D.After the surgical site was closed,an inoculum of 5×105CFU S.aureus or normal saline alone was pipetted into the joint space in group B and D or group A and C,respectively.Blood samples were collected from all groups on day 3 after the operation,the sera were serially diluted,IL-6 and C-RP were measured.The animals were sacrificed after 7 days,and X-ray,histologic analysis,scanning electron microscope(SEM) and bacterium culture was carried out to detect infection on the joint and on the removed implant.Results None of the mouse was infected in group A or group C,8 and 10 mice were infected in group B or D,respectively.The two-way ANOVA was adopted to analyse data,main effect of S.aureus on expression of IL-6 on day 3 postoperatively demonstrates a discrepancy on statistics,and implant on expression of IL-6 demonstrates no statistics difference;while main effect of S.aureus and implant on expression of C-RP on day 3 postoperatively demonstrates demonstrate no statistics difference.Conclusions A mouse model of early implant infection can be established successfully 7 days after S.aureus injecting into the joint immediately after surgery.Compared with C-RP,IL-6 measurement provides an excellent sceening test for early postoperative implant infection.

Objectives The aim of this study is to establish a scientific , simple, reproducible animal model for rapid orthodontic extrusion of subgingivally fractured anterior teeth and to test the safety of this treatment, which is a basis of further research of periodontal tissue rebuilding. Methods This research was carried out on 6 Beagle dogs. One side of the maxillary lateral incisor tooth was chosen as the experiment tooth, and the lateral incisor on the other side as control. After anesthesia, the experiment tooth was fractured 2.5 mm subgingivally to the edge of the gum. Then root canal therapy of the fractured tooth was finished at the first surgery. One week later, a metal spiral post with one end bended as a hook was put into the root canal of the tooth. Then the cast bands with an extrusion bar of the third incisor teeth at the same side was made and bonded to the tooth. Elastic rubber ring was used for orthodontic extrusion. The extrusion period was two weeks, at the end of which, the fracture line should get to the level of 1 mm supragingivally. After that, the teeth were retained in position using self-curing resin. The dogs were humanely sacrificed at the 0, 14th, and 28th day after the accomplishment of the extrusion. The samples of the fractured and control teeth with their periodontal tissues were separated. The digital X-ray images were taken. Subsequently, the specimens were made into hard tissue sections with HE staining. Results All the Beagle dogs underwent the experimental process well, with extrusion devices maintained in their positions. All the extrusions were successfully finished in 2 weeks. At the end of the extrusion, marked remodeling of periodontal ligament fibers in the experimental teeth were observed; osteoid deposition was detected at the edge of the alveolar bone. At 2 weeks after extrusion, both the periodontal lig ament fibers and the alveolar bone of the experimental teeth were in active remodeling. At 4 weeks after extrusion, the width of periodontal ligament in experimental teeth returned to normal, and the alveolar bone remodeling was basically complete. Conclusion An animal model for rapid orthodontic extrusion of subgingivally fractured anterior teeth was established in this study, which is the basis of further research in periodontal tissue rebuilding of the rapid extruded teeth.

Objective To construct a recombinant adenovirus vector for expression of proline-arginine rich 39-amino acid peptide(PR-39).Methods PR-39 gene was amplified from the plasmid containing core encoding region of PR-39 and subcloned to shuttle plasmid pAdTrace-TO4.The constructed recombinant shuttle plasmid was linearized with PmeⅠ and transfected to competent AdEasier cells.The obtained recombinant adenovirus plasmid pAdPR-39 was transfected to HEK293 cells for packaging of recombinant adenovirus which was then propagated and determined for titer.Murine fetal mesenchymal stem cells C3H10T1/2 were infected with recombinant adenovirus AdPR-39,observed for expression of red fluorescent protein(RFP) by fluorescent microscopy,and determined for transcription level of PR-39 gene by RT-PCR. Results Restriction analysis with KpnⅠ and Hind Ⅲ and sequencing proved that recombinant adenovirus shuttle plasmid pAdTrace-TO4-PR-39 was constructed correctly.Digestion of pAdPR-39 with PacⅠ proved that the target gene was integrated into the genome of adenovirus.The titer of AdPR-39 of passage 4 was l0 10 IU/ml.RFP was expressed in C3H10T1/2 cells infected with AdPR-39.PR-39 gne was successfully transcribed in C3H10T1/2 cells.Conclusion Recombinant adenovirus vector for expression of PR-39,containing RFP reporter gene,was successfully constructed and highly expressed in murine fetal mesenchymal stem cells C3H10T1/2.

OBJECTIVE: To investigate the status quo and tendency of the utilization of lipid-regulating drugs in our hospital to provide reference for clinical rational use of blood lipid-lowering drugs.METHODS: By using DDD analysis,the use of blood lipid-lowering drugs during 2008-2010 in our hospital was analyzed statistically with regard to consumption sum,DDDs and DDC(defined daily cost).RESULTS: The consumption sum of blood lipid-lowering drugs increased year by year,with statins assuming a dominating place.With distinctive mechanism of action,probucol gained more and more clinical attention and its consumption witnessed a sharp increase.CONCLUSION: The utilization structure of blood lipid-lowering drugs in our hospital is basically stable and rational.Some blood lipid-lowering drugs show bright future in clinical use.

目的 通过对维吾尔药罂粟子的生药学进行系统研究,为制定其质量标准提供试验依据.方法 对罂粟子进行性状、显微特征以及薄层色谱鉴别,采用高效液相色谱法测定吗啡的含量.结果 详细描述了罂粟子的生药性状、横切面组织构造、粉末特征和薄层色谱鉴别研究结果 ;建立了HPLC 测定吗啡的方法,并进行了方法 学验证,制定药材中吗啡含量限度为0.0005% ～ 0.01%.结论 所得结果 可以为罂粟子的生药鉴定、质量标准制定提供依据.

Objective: To assess the impact of tranexamic acid on the hidden blood loss after unilateral total hip arthroplasty.Methods:Totally 40 patients who received unilateral total hip arthroplasty due to femoral neck fracture were randomly divided into two groups.Group A included 20 cases without tranexamic acid,in which the patients were just given 250 ml normal saline when the surgery started;Group B included 20 cases with tranexamic acid,and the patients in this group were given 20 mg/kg tranexamic acid with 250 ml normal saline when the surgery started.The total red blood cell loss,visible red blood cell loss and hidden red blood cell loss of the two groups were measured and the comparative analysis was conducted.Results:Results of the visible red blood losses in group A and group B were(95.43±17.72)ml and(48.84±15.04)ml,respectively;the hidden red blood loss in group A and group B were(322.37±57.69)ml and(169.89±58.50) ml,respectively.The visible and hidden red blood cell losses in group B were both significantly less than those in group A(P0.05).Conclusion:Tranexamic acid reduces both the visible and hidden red blood loss after the unilateral total hip arthroplasty.

Senile patients with Subtrochanteric fermoral fracture frequently combine with several chronical medical disorders,and even if internal fixations are performed,it would take 5 to 8 weeks of bed ridden time to walk with partial weight-bearing,but they still faciliate high rate of severe complications,such as hypostatic pneumonia,bedsore,urinary infection,deep vein thrombosis and et al.In order to allow early walking with full weight-bearing and reduce complications,We now adapt hemiarthroplasty to treat the senile patients who have subtrochanteric fermoral fracture.

Objective To investigate the viability of rat islets through the induction by pomegranate peels extract(PPE).Methods PPE was extracted with petroleum ether and chloroform solvents,then re-extracted with ethyl acetate and obtained the ethyl acetate.Then the following experiments have been done on this extracts.That the islet cells were divided into common control group,Gilemal masculine control group,PPE groups were cultured for different days respectively,then to evaluate the viability of islet cells with the method of MTT.Results Pomegranate Extract of Ethyl acetate can promote the growth of islet cells,the viability of islet cells was markedly higher than that of control group(P0.01).Conclusions PEE contribute to improveing the viability of islet cells in vitro.

OBJECTIVE:To evaluate the effect of different resin cements and dentin surface treatment methods on shear bond strength between glass ceramic and human dentin.METHODS:Freshly extracted non-carious human premolars were collected to prepare flat mesial and distal dentin surface with diamond bur. Besides, ceramic discs (3 mm in diameter, 3 mm in height) were fabricated with IPS e. max Press ingots. All samples were randomly divided into five groups, sixteen specimens each. Ceramic discs were cemented on dentin surface using one of the five resin cements [Group A (Variolink II), Group B (Multilink Automix), Group C (Multilink Sprint), Group D (Rely X Unicem) and Group E (BisCem)] respectively. Half of the specimens were stored in water 37 degrees C for 24 h and the other half were additionally thermocycled for 5000 times (5 degrees C/55 degrees C) before shear bond strength test. Dentin bonding surface and transverse plane were examined with a scanning electronic microscope.RESULTS:Group A showed the highest bond strength before thermocycling [(22.3 +/- 3.9) MPa], and the bond strength of Group B [(18.1 +/- 3.5) MPa] was lower than that of Group A (P < 0.05). Group E [(11.7 +/- 4.2) MPa], Group C [(11.3 +/- 3.6) MPa] and Group D [(14.1 +/- 2.3) MPa] had the lowest bond strength. After thermocycling, Group A still showed the highest bond strength [(17.8 +/- 2.3) MPa]. The bond strength of Group B [(14.4 +/- 3.5) MPa] and Group D [(13.2 +/- 2.5) MPa] were lower than that of Group A. Group E [(8.9 +/- 3.2) MPa] and Group C [(7.0 +/- 2.4) MPa] showed the lowest bond strength. The bond strength of all groups decreased notably after thermocycling except Group E and Group D.CONCLUSIONS:The resin cement based on etch-rinsing bonding system has higher bond strength to dentin than those based on self-etch bonding system and self-adhesive resin cements.