Objective To study the levels of antibodies against bordetella pertussis among pregnant women and neonates in Beijing. Method From December 2016 to March 2017, pregnant women and their newborns from three women and children′s hospitals in Beijing were enrolled in this study. 3 ml of venous blood from the mothers and 3 ml of umbilical cord blood from neonates were drawn.Pertussis bacillus IgG antibody (PER-IgG) and pertussis toxin IgG antibody (PT-IgG) were tested using enzyme-linked immunosorbent assay. χ2 test was used to compare the positive rate of pertussis IgG antibodies in maternal and cord blood in the three hospitals. Correlational analyses of the antibodies levels in each hospital were conducted. The demographic characteristics, history of cough during pregnancy and history of DTaP vaccination of the mothers were collected via questionnaires. Result A total of 612 pairs of venous blood and cord blood samples were collected, including 4 mothers delivered twins and 616 cases of cord blood sample were collected. No history of pertussis were found in the 612 mothers. Among the 616 cases of umbilical cord blood, positive rate of PER-IgG was 13.3％ (82/616), positive rate of PT-IgG was 0.5％ (3/616). Among 612 cases of venous blood from the mothers, positive rate of PER-IgG was 7.7％ (47/612), positive rate of PT-IgG was 0.3％ (2/612). Positive rates of PER-IgG and PT-IgG in the mothers′ venous blood were not correlated with their residences (P=0.676 and 0.544). Positive rates of PER-IgG (r=0.842, P<0.001) and PT-IgG (r=0.619, P<0.001) in the mothers′ blood were positively correlated with the positive rate in umbilical cord blood. Conclusion This study shows that the positive rate of PER-IgG is very low in the maternal and umbilical cord blood in Beijing. Positive correlations of PER-IgG and PT-IgG between mother and umbilical cord blood were existed. Most mothers and their newborns do not have enough protection against pertussis.

Objective To investigate the outcome of intravenous immunoglobulin (IVIG) therapy in children with Kawasaki disease(KD),and to study serum tumor necrosis factor-α (TNF-α),soluble tumor necrosis factor receptor-1 (sTNFR1) levels in these patients,and to explore further treatment of IVIG non-responsive.Methods Eightythree patients with KD received initial IVIG and aspirin therapy within 10 days.Patients were divided into non-responsive group and sensitive group,while the clinical experiences and the outcome of them were recorded.Thirty-three health children were also recruited in this study,and the levels of serum TNF-α,sTNFR1 in KD patients were compared with health children and within different groups.Results 1.In non-responsive group,the rate of coronary artery lesions(CALs) was 45.4％ (5/11 cases) in non-responsive group,but 33.3％ (24/72 cases) in sensitive group,there was a statistical difference between them(P ＜ 0.05).2.Before therapy,serum TNF-α concentrations of non-responsive group were significantly higher than sensitive group[(190.7 ± 125.4) ng/L vs (104.2 ±95.5) ng/L],so there was a statistical difference between them(P ＜ 0.05).The elevation of TNF-α levels had continually existed in non-responsive group after initial IVIG therapy[(68.8 ±49.5) ng/L vs (36.8 ±28.7) ng/L],and there was also a statistical difference between non-responsive group and sensitive group (P ＜ 0.05).Serum TNF-α concentrations of non-responsive KD patients decreased to (21.2 ±7.4) ng/L after second IVIG,health children were (19.0 ± 8.3) ng/L,so there was no statistical difference between them (P ＞ 0.05).3.Serum sTNFR1 concentration was (4.6 ± 1.9) μg/L in non-responsive group,(4.7 ±2.9) μg/L in sensitive group,(5.4 ± 1.9) μg/L in health children,so there was no statistical difference between them (P ＞ 0.05).4.In non-responsive group,serum TNF-α contentrations of CALs were significantly higher than non-CALs [(266.8 ± 117.3) ng/L vs (126.2 ± 93.7) ng/L],also significantly higher than serum TNF-α contentions of CALs in sensitive group[(118.8-±96.1) ng/L],there was a statistical difference between them(P ＜ 0.05).5.In non-responsive group,but the levels of C-reactive protein (CRP) and lactate dehydrogenase (LDH) were higher than sensitive group,the levels of hemoglobin(Hb) and albumen were lower than sensitive group,so there was a statistical difference between them(P ＜ 0.05).Conclusions The high elevation of TNF-α levels existed in KD,also accompanied with abnormal sTNFR1 levels.The elevation of TNF-α levels had continually existed in non-responsive group after initial IVIG therapy.IVIG could let serum TNF-α concentrations down,so non-responsive group need additional IVIG therapy.The rate of CALs is more common in IVIG non-responsive group,but the outcome would be improved by using additional IVIG therapy.The high levels of CRP,LDH and low levels of Hb,albumen are closely associated with risk factors of IVIG re-treatment and inflammation reaction,so timely therapy is necessary to prevent damages in children with KD.

Objective To study the changes of serum TNF-αand IL-6 levels in Kawasaki disease(KD) and evaluate the relationships of them with myocardial injury and coronary artery lesions(CALs).Methods Twenty-six patients suffering from KD,11 patients with acute fever and 10 healthy children were recruited in this study.The age of KD patients ranged from 8 months to 6.10 years.The diagnosis was based on the criteria revised in 1984 by the KD Research Committee in Japan.KD patients were divided into CALs group and non-CALs group.The levels of serum TNF-α,IL-6,myocardium enzymology and cardiac troponin I(cTn-I) in KD patients were compared with healthy children and acute fever patients respectively,then compared between CALs group and non-CALs group.Doppler echocardiography were performed to evaluate coronary artery and cardiac function.Results ①Serum TNF-α and IL-6 concentrations of acute KD patients were(52.39±36.99)pg/ml and(68.64±42.33)ng/ml,significantly higher than those with acute fever patients and healthy children(P 0.01).②Serum concentrations of TNF-α and IL-6 in KD patients with CALs were(113.29±62.52)pg/ml and(150.05±44.14)ng/ml,non-CALs patients were(79.44±64.19)pg/ml and(99.49±59.20)ng/ml,CALs were higher than those with non-CALs,there was a significant statistical difference between them(P 0.05).The elevation of TNF-α and IL-6 levels had continually existed in two convalescent patients who were detected with coronary artery dilatation by ultrasound.CK-MB,left ventricular ejection fraction(LVEF) and fraction shortening(FS) were statistically significant different between CALs group and non-CALs group.③CK-MB positive percentage in KD patients was 19.2%,it significantly higher than acute fever patient and healthy children(P 0.05).④The levels of serum TNF-α were positively correlated with the levels of serum cTn-I(r = 0.675,P 0.05).Conclusions The levels of serum TNF-α and IL-6 are markedly elevated in all patients with KD,especially evidently elevated in CALs group,which has provided some evidences for the inflammation of coronary artery.High serum TNF-α concentrations are correlated with myocardial injury.The levels of serum TNF-α and IL-6 are not only closely associated with the degree of inflammation reaction,but also can be taken as an early indicator for the diagnosis of myocardium injury and CALs in KD children.

Objective To study the changes of serum IL-6,IL-8 levels and to investigate their associations with coronary artery lesions(CAL)in children with Kawasaki disease(KD).Methods From Oct.2003 to Dec.2005,46 children with KD(KD group)and 16 healthy children(healthy control group)were recruited in this study.The age of KD group ranged from 5 months to 8.75 years.KD children were divided into CAL group(18 cases)and non-CAL group(28 cases).Serum IL-6,IL-8 concentrations in KD group were compared to those of healthy control group.Results 1.The levels of serum IL-6 and IL-8 in KD group [52.39(24.39-134.46)ng·L-1,68.64(52.88-230.18)ng·L-1]were significantly higher than those in healthy control group[7.17(4.25-10.29)ng·L-1,10.34(9.60-11.57)ng·L-1](Pa0.05),and there was no correlation between them(r=-0.166,P0.1).2.The level of serum IL-6 in CAL group was significantly higher than that in non-CAL group[37.50(24.08-139.34)ng·L-1 vs 31.21(19.67-87.90)ng·L-1,P0.05].The level of IL-8 was 47.18(16.98-109.72)ng·L-1 in CAL group and 46.34(4.40-89.86)ng·L-1 in non-CAL group,there was no significant difference between them(P0.05).The elevation of IL-6,IL-8 levels had continually existed in 5 convalescent children who were detected coronary artery dilatation by ultrasound.Conclusions The levels of serum IL-6,IL-8 were markedly elevated in all children with KD,which can provide some evidences of the inflammation of coronary artery.IL-6 concentration were significantly elevated in CAL children,which might play a more important role in CAL than IL-8.When high serum IL-6 and IL-8 concentrations were observed in children with KD,CAL might develop CAL in the subacute or convalescent phase.

[Objective] To investigate the changes of vitamin A level and immune function in preschool children with recurrent respiratory tract infection (RRI). [Methods] The vitamin A level was determined with fluorometry. The percentage and Absolute count of lymphocytes subsets were measured with flow cytometry. T lymphocytes proliferation capacities and CD3/HLA-DR percentage were assayed by MTS/PMS proliferation assay and flow cytometry. The levels of cytokines and immunoglobulin were examined by ELISA and rate nephelometry. [Results] The serum VA level of RRI(0.92±0.38)μmol/L was statistically lower than that of healthy children [(1.24±0.35)μmol/L,P0.05]. The prevalence of sub-clinical syndrome vitamin A deficiency (SVAD) and suspected SVAD in RRI children was statistically higher than controls (10.4 % vs 6.8 %,P0.05 28.3 % vs 17.2 %,P 0.01 ).In RRI children the Absolute count of CD3+.lymphocytes,percentage and Absolute count of CD4+ lymphocytes,the percentage of CD3/HLA-DR and the ratio of CD4+/CD8+ were obviously lower than controls (P0.05,P0.01).The percentage and Absolute count of CD8+ lymphocytes were obviously higher than controls (P0.05). Compared Th1/Th2 cytokines in RRI children with controls,it was showed that the level of γ-INF and IL-2 were significantly decreased[(94.80±63.29) vs (172.95±70.41)ng/L,P0.05(131.52±35.47) vs (192.39±31.38)ng/L,P0.05]. Meanwhile,the level of IL-4 and IL-10 were significantly increased[(63.84±11.40 )vs (14.93±9.29)ng/L,P0.01(411.95±76.39 )vs(172.64±82.07 )ng/L ,P0.01]. Immunoglobulin G was significantly lower than controls [(5.27±3.51 )vs(7.35±3.23 )g/L,P0.05]. [Conclusions] The results suggested that vitamin A level decreased in preschool children with RRI accompanying with the imbalance of cellular immunity and the damage of humoral immunity.

Objective To study the changes of serum procalcitonin(PCT)levels and explore the role of PCT in Kawasaki disease(KD).Methods From Oct.2003 to Dec.2005,ninety-one children suffering from KD and 33 healthy children were recruited in this study.Ninety-one KD patients were divided into different groups,such as acute,subacute and convalescent groups;infection and non-infection groups;complication and non-complication groups.Serum PCT concentrations of KD patients were compared with health children,and compared with different groups.Results 1.Serum PCT concentrations of KD patients were 0.55(0.30-2.09)μg/L in acute phase,0.49(0.19-0.50)μg/L in subacute phase.There was a significant difference between them(P0.05);Serum PCT concentrations in healthy children were 0.16(0.14-0.24)μg/L,there was a significant difference between acute patients and health children(P0.01),and so did subacute patients;2.PCT levels were 0.53(0.28-0.88)μg/L in patients who had infections,and were 0.50(0.30-1.93)μg/L in patients without infection.There was not a statistical difference between them(P0.05).3.In patients with or without coronary artery dilatation,PCT concentrations were 0.50(0.32-0.60)μg/L and 0.52(0.30-2.17)μg/L,there was not a statistical difference between them(P0.05).4.Serum concentrations of PCT were significantly higher in patients with noncardiac complication as compared to those without noncardiac complication,0.83(0.48-4.41)μg/L vs 0.47(0.27-0.80)μg/L,P0.01.Noncardiac complications occurred in 22.2%(14/63)acute patients if PCT levels were ≤0.5 μg/L,the incidence increased to 42.9%(12/28)if PCT levels were 0.5 μg/L.There was a significant difference between them(P0.05).Conclusions The levels of serum PCT are markedly elevated in all patients with KD,especially in acute patients,and then gradually decreased following the development of KD.It may be a useful index for identificating stages in KD.Significantly high serum PCT concentrations are observed in KD patients with noncardiac complications,whereas the levels of PCT in patients with coronary artery dilatation are not significantly different from those without coronary artery dilatation in the acute phase.

To investigate the relationship between vitamin A deficiency(VAD) and infectious disease. Under the light proof condition, sera vitamin A(VA) concentration was determined by micro fluorescence method from the sera isolated from finger blood under strict quality control. The incidences for fever, acute respiratory tract infection(ARI), diarrhea were positively correlated with the incidence of vitamin A deficiency; the average quantity of vitamin A was negatively correlated with the incidence of fever and other diseases.[Conclusion] In China the individual or group children with low sera vitamin A, high deficiency are more vulnerable to occurrences of infectious disease. This demonstrates that vitamin A deficiency is one of the important factors related to occurrences of acute infectious diseases in children.

[目的]了解北京和贵州地区儿童维生素A缺乏(vitamin A deficiency,VAD)的现状和影响因素. [方法]从两地区各选择一市二县共1 236名儿童,其中北京随机选择城区儿童202名、农村儿童426名;贵州随机选择城区儿童207名、农村儿童401名.各地调查儿童年龄范围均为0～71个月.采用自行设计的膳食频率问卷表对每位被调查儿童询问调查前1周儿童的饮食摄入情况,并采集儿童指血200 μl,用微量荧光法检测血清VA值. [结果]城区儿童血清VA水平(平均1.27 μmol/L)明显高于农村(平均1.02 μmol/L);北京地区儿童血清VA水平(平均1.17 μmol/L)明显高于贵州地区(平均1.03 μmol/L).各地区被调查儿童家庭收入也呈现贵州低于北京、农村低于城区的状况,儿童血清VA水平和家庭收入呈正相关(r=0.239,P＜0.01).在所有调查地区中,贵州农村地区儿童血清VA水平最低(平均0.92μmol/L),家庭收入也最低(年收入平均1 800元).在调查的所有年龄组儿童中,婴儿组血清VA水平最低(28.8 μmol/L),可疑亚临床VAD发生率最高(50.0%),血清VA水平与年龄呈正相关(r=0.132,P＜0.01).儿童饮食摄入呈现明显的地区差异,动物性食品高摄入频率的比例北京地区高于贵州地区,城区高于农村;食物摄入频率较高的儿童中,可疑亚临床VAD的发生率明显低于食物摄入频率较低的儿童. [结论]儿童VAD状况与经济水平、年龄和饮食摄入有关,落后的经济状况和动物性食物摄入不足可能是导致儿童VAD的重要原因,婴儿是VAD的高危人群.

OBJECTIVE:To understand the prevalence of vitamin A deficiency (VAD) among children under six years of age in Tibet, China.METHODS:Totally, 1 257 children under six years of age were selected from two cities, two farming counties, two semi-farming counties and two livestock farming counties with stratified cluster sampling to asses VAD status in Tibet. Family information, children's feeding and disease history in the previous two weeks were collected by questionnaire. Blood specimen was collected from each child and serum was separated for detection of vitamin A concentration with microfluorescent spectrophotometry.RESULTS:Totally, 1 257 children under six years of age were surveyed, with 635 boys, 622 girls, 862 aged over two years, and 98.5% of Tibet nationality. Six cases of night blindness and two cases of xerophthalmia were detected from them, with prevalence of clinical VAD of 0.96%. Eighteen of 1071 mothers with children under six years of age were found suffering from night blindness, accounting for 1.7%. Clinical cases of VAD both in children and mothers came from all four sampling strata. Average serum concentration of vitamin A and prevalence of subclinical VAD (serum vitamin A lower than or equal to 0.70 micromol/L) was 1.15 micromol/L and 5.4% and 1.12 micromol/L and 4.7% in cities and livestock farming counties, respectively, significantly higher than those in farming (1.04 micromol/L and 11.0%) and semi-farming counties (1.05 micromol/L and 12.3%), respectively, as compared to average levels of 1.09 micromol/L and 8.4% in the autonomous region as a whole. Prevalence of subclinical VAD in children under six months and those aged six to eleven months were 22.2% and 13.3%, respectively, significantly higher than those in children aged one year (8.5%), two to three years (5.4%) and four to five years (7.9%), respectively. There was also significant difference in serum level of vitamin A between children at varied ages, but no significant difference both in serum level of vitamin A and prevalence of subclinical VAD between gender was found.CONCLUSIONS:In general, status of VAD in children of Tibet was milder than that at national level. But, moderate subclinical VAD in some areas, such as farming and semi-farming counties, did exist, so vitamin A supplementation aiming to children, especially those under one year of age, in those areas should be urged.

[目的]了解维生素A(VA)缺乏和贫血之间的关系.[方法]全国分层抽取14个省42个市县的8 699名6岁以下儿童,血清维生素A和血红蛋白的测量,分别采用荧光法及Hemocue System血红蛋白测定仪.[结果]血红蛋白正常、轻度、中度和重度贫血组儿童VA缺乏率分别为10.8%,18.7%,23.2%,42.9%,随血红蛋白水平下降显著增加.VA正常、VA可疑缺乏及VA缺乏组儿童贫血患病率分别为6.1%,12.7%,17.1%.随血清VA水平下降不仅贫血患病率增加,而且贫血严重度增加.[结论]VA和铁之间可能存在着相互影响.

Objective To provide electrocardiogram pattern of mice infected with cosackievirus for reference of experimental animal model with virus myocarditis. Methods Electrocardiographs (standard lead I, II, III, unipolar limb lead aVR, aVL, aVF ) with 0-90 Hz frequency, 50 mm/sec of paper speed were used in 260 mice (148 cases in experimental group and 112 cases in control group). Results No abnormal electrocardiograms were detected in control group, while 50% abnormal electrocariograms occurred in the experimental group, including nodal premature beat, incomplete atrioventricular dissociation, A-V block and terminal delay of QRS conduction as the infection went by. More abnormal electrocardiograms were found in young mice than in old ones. Conclusion The change in the electrocardiogram of the mice infected with coxsackievirus B_3 is conformity to the changes in the electrocardiogram of patients or animals with myocarditis. The record of mice electrocardiogram can be used as one of objective index in viral myocarditis of animal experiments.

OBJECTIVE:The survey will reveal current status of subclinical vitamin A deficiency (SVAD) and explore its affecting factors in children of China.METHODS:Totally 8 669 children aged under 6 years were randomly selected from 14 provinces for clinical examination, health and dietary questionnaire and serum level of vitamin A measurement with fluorescence method. The cut-off value for SVAD was defined as </= 0.70 micro mol/L.RESULTS:Prevalence of SVAD was 11.7% and that of suspected SVAD 39.2% in all subjects, which decreased with the increase of gross domestic product, average annual family income, mother's schooling and children's age. Prevalence of SVAD and suspected SVAD higher in rural areas (15.0%) than in urban areas (5.2%), and higher in children with a minority mother (22.6%) than in those with a Han nationality mother (8.7%). Prevalence of SVAD and suspected SVAD was higher in children whose blood samples were collected within one week in-taking vitamin A-rich food (12.6%-22.6%) than those in-taking vitamin A daily (4.1%-10.0%), and higher in children suffering from respiratory infection, fever and diarrhea two weeks before their blood collection (15.2%-20.3%) than in those without those diseases (10.1%-11.1%). Logistic regression analysis showed that poor family economic status, living in rural areas, children with a minority mother, younger age, no-dairy milk products intake, and respiratory infection and fever all were risk factors for SVAD.CONCLUSIONS:More than half of children under six years old in China (50.9%) had vitamin A nutrition problem. Varied factors played roles to different extent in SVAD in children.

There was no available basic data on serum vitamin A(VA) content among children in China.The survey will reveal serum VA status in 0～6 years children of China. Randomly selected 8 669 children aged under 72 months in 14 provinces for questionnaire and serum VA content measured.The cut off for subclinical vitamin A deficiency(SVAD) being≤0.70μmol/L. The serum VA mean levels of children were(1.06±0.33)μmol/L.The prevalence of SVAD were 11.7% in subjects.The younger(0.84μmol/L),the worse diet(0.93～1.04μmol/L),the higher incidence of diseases(0.87～1.23)μmol/L,the lower family and local annual income(0.90～0.97μmol/L) were,the lower serum VA levels were.In addition,the children of their mothers with low education(0.93μmol/L) and minority nationality(0.91μmol/L) had lower serum VA levels. [Conclusions] The serum VA content were lower among 0～6 years children in China.Multiple factors need to be considered together for them,such as age,diet,illness,annual income,education status and nationality of mother.

Objective As one of the most common clinical methods,electrocardiogram can be used to observe the developing process,if animal model serves,and to provide a basis for pathological mechanism.Authors studied electrocardiograms of normal mice and summed up normal pattern for experimental animal models,and clinical pharmacological study.Methods Electrocardiograph with 0-90 Hz frequency and 50 mm/sec paper speed were recorded in standard lead of Ⅰ,Ⅱ,Ⅲ and aVL,aVR,aVF unipolar limb lead in 312 mice.Results The different patterns of electrocardiograms of normal mice were summed up.The electrocardiogram had no significant difference among different ages of mice older than 35 days with the average heart beat rate of 354.22±49.86 beat/min,electrical axis of 71.64°±10.3° degree,P-R interval of 0.036±0.005sec, p wave voltage of lead Ⅰ of 0.079±0.025 mV and duration of lead Ⅰ of 0.017±0.003 sec and duration of QRS complex of 0.021±0.005 sec.Conclusion The electrocardiogram of normal mice provides the basis for comparing the electrocardiogram of normal mice with that of infected mice and is for reference in determination of the process of infection of mice.

测定不同时期病毒性心肌炎患儿的细胞免疫,以探讨其临床意义,将92例病毒性心肌炎患儿和30例对照组细胞免疫检测结果进行比较.结果显示,心肌炎急性期和慢性期的细胞免疫(CD3、CD4、CD8和B细胞)与对照组比较,差异有显著性(P<0.01);恢复期CD4/CD8存在显著差异(P<0.05);迁延期CD4和B细胞存在显著差异.表明病毒性心肌炎免疫改变以细胞免疫为主,存在免疫细胞功能紊乱,于急性期及慢性期尤为显著,恢复期好转,迁延期存在T细胞亚类比例失衡.

病毒性心肌炎系病毒感染引起的局灶性或弥漫性的心肌间质炎症或坏死的病变,导致不同程度的心功能障碍和周身症状的疾病.引起心肌炎的病毒已证明的有20余种,以肠道病毒包括柯萨奇A、B组病毒,脊髓灰质炎病毒,埃可病毒等为常见,尤其是柯萨奇B组(CVB)最多.此外,腺病毒、EB病毒、流感病毒、肝炎病毒、疱疹病毒、腮腺炎病毒、麻疹病毒、巨细胞病毒等均可引起心肌炎.

OBJECTIVE:To understand the prevalence and spatial distribution of vitamin A deficiency (VAD) in China among children at ages under six years.METHODS:About 8,600 children under 6 years of age in 14 cities and 28 counties of 14 provinces were selected with stratified cluster sampling for survey, including interview with questionnaire for their family information and nutritional status. Blood specimens were collected for measuring serum level of VA with fluorescent spectrophotometry in laboratory.RESULTS:Totally, 8,669 children under 6 (2,877 in urban area and 5,792 in rural area) were surveyed in 14 provinces, with 4,629 males and 4,040 females. Eight cases of night blindness and seven cases of xerophthalmia were found among the children at ages of two to five years. Sixty-one mothers of the children in this group were also found suffering from night blindness. All the cases of night blindness and xerophthalmia both in children and mothers were living in rural areas. Based on their serum levels of VA, 11.7% of the all 1 018 children were diagnosed as VAD, with serum VA concentrations below or equal to 0.70 micro mol/L. Prevalence of VAD was 15.0% and 5.8% in rural (23.3% in the poverty-stricken counties) and urban areas, respectively, and 5.8%, 11.5% and 16.8% in the coastal, inland and remote areas, respectively. The average serum level of VA was 1.20 micro mol/L and 0.99 micro mol/L for urban and rural areas, respectively, with a national average of 1.06 micro mol/L. And, babies under six months of age with an average serum levels of VA < or = 0.70 micro mol/L accounted for 33.4%, and those at ages of four to five years with the same level of VA accounted for 8%. There was significant difference in serum levels of VA between ages, but no significant difference between genders.CONCLUSION:VAD did exist in children of China, especially in the remote and poverty-stricken rural areas and VA supplementation is urgently needed for the children in these regions.

OBJECTIVE:To study the specificity of IgM antibody response in patients with Coxsackie virus B infection.METHODS:A Coxsackie virus B specific immunoblot assay was established for detection of CVB IgM antibody response in patients with recent CVB infection.RESULTS:Of 28 patients' serum samples, positive by ELISA captured CVB IgM test, analysis results of specific IgM response defined by immunoblot assay revealed VP1 protein specific which was crossly reactive among CVB group.CONCLUSION:This finding suggested that VP1 protein in Coxsackie virus B group potentially functioned as a predominant antigen inducing detectable IgM antibody following CVB infection.

为使血清维生素A(VA)微量荧光测定法适用于大规模调查,我们在美国疾病控制中心(CDC)健康和营养监测(NHANES)实验室Sowell Anne博士指导下,改进了该方法并将其用于我国14省8 669名学前儿童的血清VA测定.

河南省汝阳县呼吸道、消化道感染性疾病发病率较高,且每隔数年即有流行,为了获得这一地区0～10岁儿童维生素A低下及贫血发病率,维生素A缺乏与贫血、呼吸道及消化道感染、辅食添加状况相互关系的资料,由首都儿科研究所和河南省汝阳县人民医院合作进行了调查.