【Objective】The influencing factors of rabbit embryo transplantation were explored to provide references for improving rabbit embryo transplantation efficiency and producing transgenosis rabbits. 【Method】The embryo were collected after mating and cultured in vitro, and then transplanted into recipients according to the number of embryo, recipients type(young hare multiparous hare) and synchronic oestrus methods. The pregnancy rate was observed after transplantation. 【Result】The results showed that 10-20 or 20-30 embryos were respectively transferred into each recipient. There was no significant difference in the pregnancy rate between the two groups(23.1% vs 20.0%, P0.05). When the multiparous rabbits were used as recipient, the pregnancy rate was significantly higher than that of nulliparity rabbits(27.8% vs 13.3%,P0.05). Embryos were respectively transferred into natural estrus recipients and induced estrus recipients. The pregnancy rate of natural estrus recipients was extremely higher than that of induced estrus recipients(30.7% vs 10.0%, P0.05).【Conclusion】These results suggested that the natural estrus of multiparity rabbits were better to be used for recipients than nulliparity rabbits for ET, and the 10-30 transfer embryos were the best.

Embryonic stem(ES)cells are derived from the inner cell mass of early blastocyst, and possess the ability of self-renewal and maintenance pluripotency.Wnt signaling pathway plays important roles in maintaining self-renewal of ES cells.Glycogen synthase kinase-3(GSK-3) inhibitors can active Wnt signaling pathway,then promote the self-renewal and cellto-cell adhesion of ES cells.In this paper,we take a review for the effect and mechanism of GSK-3 inhibitors on maintaining self-renewal and cell-to-cell adhesion of ES cells.

To study the function of GLUT3,a pair of primers was designed based on the nucleotide sequences of GLUT3 gene from bovine,human and other species published in Genbank. The total RNA from ovarian tissue in swamp buffalo was used as a template,and then the GLUT3 cDNA encoding the mature protein was amplified using reverse transcription polymerase chain reaction( RT- PCR). The amplified product was cloned into pMD 18- T vector,and then was used for PCR,the identification of double enzymes digestion,and sequencing analysis. The results showed that the coding sequence length of the amplified GLUT 3 gene in swamp buffalo was 1485 bp,encoding 494 amino acids predicted by biology- related software. The ORF sequence of the GLUT 3 exhibited high homology with cattle( 100%),sheep( 100%),human( 99%),mouse( 94%)and pig( 81%),respectively. Its protein sequence exhibited high homology with cattle( 100%),sheep( 99%),human( 99%),mouse( 99%) and pig( 97%),respectively. The results indicate that GLUT3 belongs to a group of highly conserved proteins in evolution.