To examine the effect of sodium selenite in different concentration on the body weight,superovulation and embryonic developmental competency in mouse,the 6～7 weeks-age mice were randomly divided into four groups and treated with different concentration of sodium selenite solution,such as 0(control group),0.30 mg/kg(low dose group),1.50 mg/kg(medium dose group),2.25 mg/kg(high dose group),respectively.And then the body weight of mice was measured,as well as the number of embryos derived from superovulation and the in vitro developmental rate of embryo was recorded in each group.The results showed that the change of the mice body weight in high dose group(-0.31 g) was significantly lower than those of other three groups(control: 11.06 g,low: 7.95 g,medium: 8.65 g,P0.05),but there was no remarkably different among the low dose group,medium dose group and control group(P0.05).There was no significant difference in the number of embryos derived from mice superovulation among the four groups(P0.05).Compared with the control group,the blastocyst developmental rate of embryo from superovulation by in vitro culture was significantly decreased in high dose group(17.6% vs 27.6%,P0.05),but it had no significant difference with the low dose group(24.3%) and medium dose group(20.6%)(P0.05).These results indicated that high doses of sodium selenite had toxicity on embryonic developmental ability,and its mechanism should be took an intensive study at the cellular and molecular level.

Embryonic stem(ES)cells are derived from the inner cell mass of early blastocyst, and possess the ability of self-renewal and maintenance pluripotency.Wnt signaling pathway plays important roles in maintaining self-renewal of ES cells.Glycogen synthase kinase-3(GSK-3) inhibitors can active Wnt signaling pathway,then promote the self-renewal and cellto-cell adhesion of ES cells.In this paper,we take a review for the effect and mechanism of GSK-3 inhibitors on maintaining self-renewal and cell-to-cell adhesion of ES cells.

To study the function of GLUT3,a pair of primers was designed based on the nucleotide sequences of GLUT3 gene from bovine,human and other species published in Genbank. The total RNA from ovarian tissue in swamp buffalo was used as a template,and then the GLUT3 cDNA encoding the mature protein was amplified using reverse transcription polymerase chain reaction( RT- PCR). The amplified product was cloned into pMD 18- T vector,and then was used for PCR,the identification of double enzymes digestion,and sequencing analysis. The results showed that the coding sequence length of the amplified GLUT 3 gene in swamp buffalo was 1485 bp,encoding 494 amino acids predicted by biology- related software. The ORF sequence of the GLUT 3 exhibited high homology with cattle( 100%),sheep( 100%),human( 99%),mouse( 94%)and pig( 81%),respectively. Its protein sequence exhibited high homology with cattle( 100%),sheep( 99%),human( 99%),mouse( 99%) and pig( 97%),respectively. The results indicate that GLUT3 belongs to a group of highly conserved proteins in evolution.

【Objective】Differ ent expression profiles of polypeptides in follicle fluid from superovulated cattle and calves were studied in order to provide references for further research on follicle fluid of calves and in vitro oocyte culture system of calves.【Method】The matrix-assisted laser desorption ionization mass spectrometry(MALDI-TOF MS)was used to analyze the peptide fragments in follicular fluid of superovulated cattle and calves.Based on signal peak information of polypeptide,protein search was conducted in MASCOT database.【Result】The models of polypeptide expression profiles of the two groups were successfully established,respectively.There were 30-40 peak signals in cattle group,and 60-80 peak signals in calves.By comparing the peak lists from the two different samples,it was found that 2 specific peaks of 1218.58 and 1509.77 m/z appeared in cattle group,similarly,and 1 specific peak of 953.54 m/z in calf group.At last,MASCOT library was used to search the information of polypeptides,and then some proteins related to these polypeptides information were gained.The results showed that 18 kinds of proteins existed in cattle group,and 11 kinds of proteins in calves.【Conclusion】The results showed that the polypeptide information peak quantity of calf follicular fluid protein was higher than that of adult cattle,while the total number of proteins in follicle fluid from the calves was less than that of the adult cattle group,which could be one of reasons why the developmental competence of oocytes from calves was poorer than that of adult cattle.

In order to estabilish and optimize a two dimensional gel electrophoresis(2-DE)technique for proteome analysis of calf follicular fluid,effects of two different IPG strips and 4 kinds of loading volumes(150 μg,250 μg,350 μg,450 μg) on the quanty of the 2-D maps were studied.The results showed that one perfect and clearly 2-DE map was obtained when pH 4~7 IPG strip with 24 cm and loading volume of 350 μg were used.About 319 spots in the 2-DE map were detected by the Image Master 2-D platinum software.Our established 2-DE technique of calf follicular fluids can be effectively applied in different peoteins between calves and adult cattles.

Methylation plays an important regulatory role in embryo development and organization.Somatic nuclear transfer embryos often show the abnormal mode of DNA methylation,DNA methyltransferase inhibitors can inhibit the levels of DNA methylation,so as to improve the efficiency of SCNT.The application of DNA methyltransferase inhibitors in animal somatic cell nuclear transfer was taken an overview in this paper.

以ZORLESCO(-BSA)稀释液为公猪精液常温保存基础稀释液,研究聚乙烯吡咯烷酮(PVP)、聚乙烯醇(PVA)含量对17℃公猪精液保存的影响。结果发现,在ZORLESCO(-BSA)稀释中液添加0.4%PVP和0.4%PVA保存的效果较好,其中以0.4%PVP保存效果最佳。