以甘肃天水产成熟适中的红元帅、富士苹果经循环提香加热分离和提纯所得气体混合物为实验研究对象,对苹果汁中的芳香组分进行回收、精馏、浓缩后,抽取代表性样品进行气相色谱分析,采用带有FID检测器的气相色谱仪,用内标法定量对天然苹果香精的香气组分及其代表性理化特征指标进行分析,对客户关注的天然苹果香精的主要质量评价指标的测定方法做了系统研究,并制定了高倍浓缩天然苹果香精的企业标准,在甘肃省质量技术监督局做了备案,作为行业代表性科技成果,对我国高倍浓缩天然苹果香精行业的生产经营具有重要指导意义和实践参考价值。

In this study,the new extraction technique of soybean bioactive minor peptides from defatted soy flakes hydrolysized by crude complex trypsinase and neutral proteinase was set up.Optimal condition of pretreatment and hydrolyzing by crude complex trypsinase was given by enzyme quantity of 9 000 U·g-1,temperature 55℃,pH 8.5,time 6 h,under the condition of 5.0% substrate concentration.And the optimum condition of neutral proteinase hydrolysis was given by enzyme quantity 8 500 U·g-1,pH 7.5;temperature 45℃;treatment time 2 h.Under this condition,the ratio of soybean minor peptides reached 73.98 %.And the quality standards of soybean bioactive minor peptides were also established.