Objective To assess the clinical value of compression-braking pants in preventing postoperative bleeding and pressure sore after transcatheter arterial chemoembolization (TACE).Methods A total of 50 patients with liver cancer,who received interventional treatment via femoral artery route at authors' hospital during the period from May 2015 to May 2016,were enrolled in this study.The patients were randomly divided into observation group and control group with 25 patients in each group.In the observation group,the patients were asked to put on the compression-braking pants after TACE procedure and routine postoperative nursing care was executed as usual.In the control group,routine postoperative nursing care and compression of puncture point with sandbag were carried out.After TACE,the conditions of local congestion,local hematoma,formation of pseudoaneurysm at the puncture site,etc.in both groups were recorded.The local skin conditions,including skin moist feeling,burning sensation,feeling of numbness and rubefaction were determined.Results The postoperative bleeding incidence in the observation group was 4％,which was obviously lower than 32％ in the control group.No pressure sore occurred in both groups.In aspect of skin moist feeling,burning sensation,feeling of numbness and rubefaction,the observation group was significantly superior to the control group (P＜0.05).Conclusion The use of compression-braking pants after TACE can effectively prevent the occurrence of postoperative bleeding and pressure sore.The compression-braking pants have reasonable design and simple structure,and it is easy to put on and take off the pants.The use of the pants can improve patient's comfort and satisfaction.Therefore,it is worth promoting its use in clinical practice.

Objective To explore the application effects of pressure brake pants on patients with liver cancer after transarterial chemoembolization (TACE).Methods The study recruited 50 liver cancer patients who were admitted to Lianyungang No.2 people's hospital from May 2015 to May 2016 to receive TACE. The participants were randomly divided into the intervention group (n=25) and the control group (n=25). Based on the routine nursing care,patients in the intervention group also had to wear the pressure brake pants. The general comfort questionnaire (GCQ) was applied to assess the comfort level. Comparing the comfort and satisfaction between the two groups.Results The intervention group had higher GCQ score six hours after the operation compared with the control group (P<0.05). Besides,patients of the intervention group were much more satisfied with the nursing care compared with the patients in control group (92% vs. 64%,P<0.05).Conclusions The pressure brake pants can effectively improve patients' comfort level and prevent the incidence of pressure sores. Moreover,they are good for privacy protection and high quality of nursing care.

Objective To study the effect of Ro60 on migration and invasion of lung adenocarcinoma A 549 cells. Methods We knocked down Ro60 and analyzed the ability of migration and invasion of A 549 cells.Quantitative real time ( RT)-PCR and Western blotting were performed to detect mRNA and protein levels of selected molecules associated with migration and invasion of neoplasm .Results When Ro60 was downregulated , the migration and invasion of A 549 cells decreased markedly.Meanwhile,the mRNA expression of matrix metalloproteinase (MMP)9,c-Src etc was observably reduced.Furthermore, downregulation of Ro60 diminished the expression of Src protein and the activation of MMP-9 protein.Conclusion Downregulation of Ro60 inhibits migration and invasion of A549 cells by regulating Src protein and activating MMP-9 protein.

This study focuses on cold-pressed oil cakes of camellia and bancoul nut seeds, studying the fractal characteristics of pore microstructure of these two oilseed cakes. Combined with the fractal theory, the Darcy law and Hagen-Poiseuille formula were applied to establish a fractal permeability model of oil seed cakes. Cold press was used to determine permeability of oil cakes of camellia and bancoul nut seeds. The calculated values obtained through the model were compared to the experimental values. The relative average errors for camellia and bancoul nut oilseed cakes were 11.7% and 8.7%, respectively.

Aiming at cold-pressed oil cakes of linseed and sunflower seed, the pore fractal characteristics of microstructures have been studied. By combining Hagen-Poiseuille equation with fractal theory, a fractal permeability model for oil cake has been developed. The permeability of linseed and sunflower seed cakes under cold-pressed condition has been measured. It was found that the relative average errors are 12.8% and 16.8% for the soybean cake and sunflower seed cake respectively by comparing the result from the model calculation and experiment.

DNA damage responses(DDR) cellular defense mechanism acts as a potent barrier for tumor initiation and progression,containing transcriptional regulation,cell cycle checkpoint activation,DNA damage repair,apoptotic and non-apoptotic cell death,which is the cornerstone of genome stability.Dysfunction of DDR signaling is associated with genomic instability diseases,such as tumor,senescence and complex diseases.The promyelocytic leukemia protein(PML) is the indispensable scaffold of PML nuclear bodies(PML-NBs),which are matrix associated multi-protein subnuclear structures.Previous studies have showed that PML-NBs are dynamic sensor of DNA damage,and regulate a wide variety of biological processes,including chromatin remodeling,epigenetic modification,gene transcription and post-transcriptional RNA stability and translation,post-translational protein modification and location.PML-NBs function as pivotal hub of signal transduction and network coordination in p53-dependent and p53-independent checkpoint activation,damage repair and apoptosis induction in DNA damage responses(DDR).

An increasing data indicates that altered microRNAs(miRNAs)participate in the radiation-induced DNA damage response.However,a correlation of mRNA and miRNA profiles across the entire genome and in response to irradiation has not been thoroughly assessed.We analyzed miRNA microarray data collected from HeLa cells after ionizing radiation(IR),quantified the expression profiles of mRNAs and performed comparative analysis of the data sets using target prediction algorithms,Gene Ontology(GO)analysis,pathway analysis,and gene network construction.The results showed that the altered miRNAs were involved in regulation of various cellular functions.miRNA-gene network analyses revealed that miR-186,miR-106b,miR-15a/b,CCND1and CDK6 played vital role in the cellular radiation response.Using qRT-PCR,we confirmed that twenty-two miRNAs showed differential expression in HeLa cells treated with IR and some of these miRNAs affected cell cycle progression.This study demonstrated that miRNAs influence gene expression in the entire genome during the cellular radiation response and suggested vital pathways for further research.

Objective To investigate the expression and subcellular localization of RNA-binding protein Ro60 in neoplasms before and after γ-ray irradiation,and the function of Ro60 in tumor cell proliferation and radio-sensitivity.Methods The eukaryotic expression plasmid of mGFP-Ro60 was constructed and transfected into HCT116 and MCF7 tumor cells.The cellular localization of Ro60 was examined before and after irradiation.Cell proliferation and radio-sensitivity were detected by CCK8 and trypan blue assay.Results The result of immunoblotting showed that tumor cells expressed Ro60 protein.The irradiation increased Ro60 expression and induced significantl nuclear aggregation of Ro60.The cell proliferation before and after irradiation was drastically reduced while cell death increased in Ro60 overexpressed tumor cells.Conclusion γ-ray irradiation alters Ro60 expression and localization,and Ro60 plays an important role in tumor cell proliferation and radio-sensitivity.

以冷态压榨成型的大豆和棉籽油料饼为研究对象,对两种油料饼的孔隙微观结构进行分形特性研究,采用Hagen-Poiseuille公式结合分形理论建立植物油料饼渗透率分形模型,以冷态压榨方式实际测定大豆和棉籽油料饼的渗透率。对模型计算值与试验测试值进行对比得到大豆饼模型的相对平均误差为18.7%,棉籽饼模型的相对平均误差为12.4%。

Objective: To analyze the common antigenic epitope of mouse vascular endothelial growth factor (VEGF) 164 and human VEGF165 and lay a foundation of design and verification of VEGF epitope vaccine. Methods: The candidate common epitope of VEGF164 and VEGF165, EYPDEIEYIFKP, was determined by analyzing the interaction of VEGF and its receptor KDR. The common epitope was inserted into skeleton protein. The skeleton protein gene containing the common epitope was amplified and inserted into prokaryotic expression vector pET-24a. The recombinant plasmid pET-24a-FV was transformed to E. coli BL21(DE3) for expression under induction of IPTG. The expressed product was purified by Sephacryl S-100 chromatography, then analyzed by SDS-PAGE. BALB/c mice were immunized with the purified epitope display protein and determined for serum antibody titer by ELISA, and for recognition to mouse VEGF164 and VEGF165 by Western blot and IFA. Results: The common antigenic epitope was the 40S Loop of KDR binding region. Restriction analysis and sequencing proved that recombinant plasmid pET-24a-FV was constructed correctly. Epitope display protein was mainly expression in a form of inclusion body, which contained 20% of total somatic protein and reached a purity of 92%. Both moue VEGF164 and human VEGF165 showed reactions with immune sera of mice, with a titer of 1:104. The immune sera recognized not only epitope display protein, but also the monomers and dimmers of mouse VEGF164 and human VEGF165, and showed reactions with human HeLa and mouse B16 cells, indicating that the epitope display protein could display the common antigen epitope of VEGF. Conclusion: The common epitope of VEGF164 and VEGF165 was displayed by skeleton protein and induced the corresponding specific immune responses in mice, suggesting that EYPDEIEYIFKP was the common epitope of VEGF164 and VEGF165.

Considering the fractal characteristic of oilseed cake, the relationship between the permeability and the pore fractal dimension of peanut and sesame cake has been investigated. The microstructures of peanut and sesame cake under five applied pressures are measured by using stereo light microscope and Image-pro image analyzer. Using the box-counting method, the fractal dimensions of pore size distributions are measured. A mathematical model incorporated fractal dimension and permeability has been developed to predicate the permeability of compressed peanut and sesame under cold condition based upon combining Hagen-Poiseulle equation with Darcy’s law for flow of fluid through porous media. There is a prediction of permeability of peanut and sesame cake. Thus, a measurement is carried out for validation. The values of mean relative errors are 19.4% and 11.4 respectively. A fairly good agreement is obtained in the case of high applied pressure. And there exists a tendency that the value of the difference between the theoretical calculation and the permeability measurement decrease significantly with the increase of applied pressure.

目的:建立一种能够有效展示蛋白质B细胞表位的载体系统,以用于表位特异性抗体的制备和表位疫苗的设计。方法:选用抗体的可变区作为蛋白质B细胞表位展示的骨架蛋白,B细胞表位的展示部位为CDR3区。全基因合成骨架蛋白基因,通过重叠PCR将B细胞表位编码序列插入到骨架蛋白基因中,原核表达目的蛋白,利用Sephacryl S-100层析柱进行蛋白纯化,用纯化的蛋白按常规方法免疫小鼠,采用ELISA法检测免疫血清的滴度及特异性,通过Western blot和间接免疫荧光技术进一步验证免疫血清对目的蛋白的识别。结果:成功构建了3种表位展示蛋白,均表现出了很好的抗原性,表位展示蛋白免疫血清具有较好的特异性,能够特异识别所展示的B细胞表位。结论:抗体可变区作为骨架蛋白能够很好地展示B细胞表位,免疫小鼠后获得的免疫血清表现出了较好的特异性。

microRNAs (miRNAs) are a versatile class of non-coding RNAs involved in regulation of various biological processes. miRNA-122 (miR-122) is specifically and abundantly expressed in human liver. In this study, we employed 3′-end biotinylated synthetic miR-122 to identify its targets based on affinity purification. Quantitative RT-PCR analysis of the affinity purified RNAs demonstrated a specific enrichment of several known miR-122 targets such as CAT-1 (also called SLC7A1), ADAM17 and BCL-w. Using microarray analysis of affinity purified RNAs, we also discovered many candidate target genes of miR-122. Among these candidates, we confirmed that protein kinase, interferon-inducible double-stranded RNA-dependent activator (PRKRA), a Dicer-interacting protein, is a direct target gene of miR-122. miRNA quantitative-RT–PCR results indicated that miR-122 and small interfering RNA against PRKRA may facilitate the accumulation of newly synthesized miRNAs but did not detectably affect endogenous miRNAs levels. Our findings will lead to further understanding of multiple functions of this hepato-specific miRNA. We conclude that miR-122 could repress PRKRA expression and facilitate accumulation of newly synthesized miRNAs.

Ionizing radiation (IR) causes severe cellular damage both directly and indirectly and disrupts RNA integrity. RNA strand breaks are the most frequent type of damage caused by IR. RNA damage is involved in the development of degenerative diseases, including Alzheimer’s disease and Parkinson’s disease. However, the mechanism of mRNA damage and any resulting pathophysiological outcomes are poorly understood. This is partly because there is a lack of sensitive tools to monitor damage randomly occurring in RNA, especially RNA strand break damage in a given RNA. In this work, a method using the reverse transcription polymerase chain reaction (RT-PCR) after poly(A) addition to 3′-end of RNA to determine RNA strand break damage in a specific RNA by poly(A) polymerase has been developed. The levels of damage in specific mRNAs, including ABL1, TP53, GADD45A and ATR from IR-treated HeLa cells were examined. Strand breaks were detected in all mRNAs examined. The study provides a novel and sensitive method based on 3′-end poly(A)-tailing RT-PCR to monitor RNA strand break damage.

By using of Kuhn's yield criterion, plasticity constitutive equations of extruded oilseeds in a closed cylinderical cell were developed. The model parameters were identified from experimental stress-strain using an inverse method. The maximum relative deviations between the measured and the simulated value of soybean and cottonseed are 8.5% and 5.1% respectively, and the average relative deviations are 4.9% and 3.8% respectively. The results of numerical simulation for confined pressing of granular soybeans and cottonseeds in the closed cylinderical cell indicated the following facts that granular soybeans and cottonseeds conform to the model of three power curve, the plasticity constitutive equations can describe the plastic deformation for extruded soybean and cottonseed, and Kuhn's yield criterion can be used as theoretical basis for plasticity model of granular soybeans and cottonseeds.

The cyclin-dependent kinase inhibitor p21WAF1/Cip1 is a critical cell cycle regulator which translocates into the nucleus to participate in DNA repair during DNA damage responses. In the present study, we showed that the tumor suppressor, promyelocytic leukemia protein (PML) contributes to the up-regulation of p21 in a p53-independent pathway. Knock-down of PML in p53-null H1299 and HCT 116 (p53−/−) tumor cells by specific siRNA resulted in down-regulation of p21 protein expression, inhibition of γ-irradiation-induced p21 up-regulation, and a decrease in p21 protein half-life. In PML knockdown H1299 cells, the down-regulation of p21 protein expression was reversed by MG132 treatment indicating that the proteasomal degradation of p21 protein was increased. Thus, PML positively regulates p21 expression by inhibiting proteasome-mediated proteolysis. Knockdown of PML decreased the repair of γ-irradiation-induced double strand breaks (DSBs) as indicated by the delayed disappearance of γ-H2AX foci and a decreased association between p21 and proliferating cell nuclear antigen (PCNA). Over-expression of p21 significantly restored the delayed DSB repair function. Taken together, these data provide evidence for a p53-independent functional relationship between PML and p21 in γ-irradiation-induced DNA damage responses, and identify PML as a positive post-translational regulator of p21 in p53-deficient tumor cells.

Lung resistance-related protein (LRP) has roles in multi-drug resistance of tumor cells. Understanding the mechanisms that regulate LRP expression in tumor cells is an important research area. A putative p53 response element in the LRP promoter has been found. Thus, p53-related regulation of LRP expression was explored in this study. We first demonstrated that p53 overexpression inhibited LRP expression both at the protein and mRNA levels. Then, using a dual-luciferase reporter assay, we located the p53 response element to the Y-box (-263 similar to-407) of the LRP promoter, the YB-1 binding site, but not the putative p53 response element. Furthermore, coimmunoprecipitation and chromatin immunoprecipitation showed p53 could bind to the Y-box of the LRP promoter through interaction of p53 with YB-1. YB-1 coexpression with p53 facilitated p53-induced suppression of endogenous LRP expression in MCF-7 cells. HDAC2, a corepressor of p53, was found to also interact with YB-1, and this interaction was mediated by p53. These results showed that the p53-HDAC2 transcriptional repressor complex can bind to the Y-box of the LRP promoter and repress LRP expression through interaction with YB-1. p53-related suppression of LRP expression was completely reversed by doxorubicin treatment and Adr, whereas CP and VP-16 treatment induced LRP expression increased significantly. Inhibition of LRP expression by siRNA facilitated Adr induced apoptosis of MCF-7 cells. All these findings indicated that loss of p53-related suppression of LRP may be the reason for LRP expression increase, and, therefore, chemotherapy resistance in tumor cells. J. Cell. Physiol. 226: 3433-3441, 2011. (C) 2011 Wiley Periodicals, Inc.

MicroRNAs(miRNAs)are small single-stranded noncoding RNAs consisting of 21-23 nucleotides that play important gene-regulatory roles in eukaryotes by pairing to the mRNAs of protein-coding genes to direct their posttranscriptional repression.A growing body of evidence indicates that alterations in miRNA expression may occur following exposure to several oxidative stress including ionizing radiation.So miRNAs may serve as potential new targets for co-therapies aiming to improve the effects of radiation disease therapy in cancer patients.The progress in research on ionizing radiation-induced miRNAs is reviewed in this paper.

We show here that γ-irradiation leads to the translocation of endogenous Werner syndrome helicase (WRN) from nucleoli to nucleoplasmic DNA double strand breaks (DSBs), and WRN plays a role in damage repair. The relocation of WRN after irradiation was perturbed by promyelocytic leukemia protein (PML) knockdown and enhanced by PML IV over-expression. PML IV physically interacted with WRN after irradiation. Amino acids (a.a.) 394 to 433 of PML were necessary for this interaction and the nucleoplasmic translocation of WRN and were involved in DSB repair and cellular sensitivity to γ-irradiation. Taken together, our results provide molecular support for a model in which PML IV physically interacts with and regulates the translocation of WRN for DNA damage repair through its 394–433 a.a. domain.

It is well established that promyelocytic leukaemia nuclear bodies (PML NBs) play important roles in DNA damage responses (DDR). After irradiation, PML NBs dynamically recruit or release important proteins involved in cell-cycle regulation, DNA repair and apoptosis. As PML protein is the key molecule of PML NBs' dynamic assembling, we aimed to characterize the PML-interacting proteins in (60)Co-irradiated MCF-7 cells. A proteomic approach using CoIP, mono-dimensional electrophoresis and tandem mass spectrometry, allowed us to identify a total of 124 proteins that may associate with PML after irradiation. Bioinformatic analysis of the identified proteins showed that most of them were related to characterized PML functions, such as transcriptional regulation, cell-cycle regulation, cell-death regulation and response to stress. Four proteins, B23, MVP, G3BP1 and DHX9, were verified to co-localize with PML differentially before and after ionizing radiation (IR) treatment. The proteins identified in this study will significantly improve our understanding of the dynamic organization and multiple functions of PML NBs in DDR.