OBJECTIVE To establish a method for simultaneous determination of Prednisone and Prednisolone in the blood and urine.METHODS The compounds were extracted from blood and urine using ethyl acetate as the solvent for liquid-liquid extraction and detected by HPLC with Betamethasone as the internal standard.Separation was achieved on Zorbax SB-Aq column using methanol-0.01 mol·L-1 potassium dihydrogen phosphate(57:43) as mobile phase.RESULTS The linear limits of Prednisone and Prednisolone ranged from 0.05 μg·ml-1 to 40.00 μg·ml-1 in the blood(r≥0.9997)and 0.04 μg·ml-1 to 50.00 μg·ml-1 in urine(r≥0.9991),respectively.The limits of detection for these two analytes were 0.012 μg·ml-1 and 0.008 μg·ml-1 in the blood and urine,respectively.The intra and inter day assay precision of this method were no more than 7.8%.The mean recovery of Prednisone and Prednisolone in the blood and urine ranged from 92.9% to 106.3%,and the mean extraction recovery of these two analytes was no less than 85.4%.CONCLUSION The method is accurate,sensitive,well reproducible and suitable for simultaneous determination of Prednisone and Prednisolone.

胎儿纤维结合素是细胞外基质中一种由种植的滋养层组织分泌的糖蛋白,含有称为"癌胚区"的抗原决定簇,可被单克隆抗体FDC-6识别.孕20～37周宫颈阴道分泌物中的胎儿纤维结合素可能是一个对早产有良好诊断、预测价值的标记物.尤其是其阴性预测值高,有助于避免不必要的医学干扰.