In this paper,in order to improve the forecast accuracy,put forward a kind of improved method of least squares loss forecast technology.Algorithm for the first time the kernel partial least squares regression algorithm is applied to the line loss rate prediction.Experiments show that,this method can overcome the variable correlation and prediction model of nonlinear factors on adverse effects.

The accurate synchronization signal at the receiving end is obtained and chaos synchronization of two single ring laser is achieved through numerical simulation by master-slave method. It is founded that chaos synchronization of Erbium-doped fiber ring laser with master-slave can be realized and synchronization is unconcerned in initial system. These results will be useful to control and application of chaos in secret communication.

After introducing the design method of digital current (DC) power supply and the circuit schematic diagram and software flow chart of the key part, the paper designs the core processor based on ARM 32-bit Cortex™-M3 as controller, which, in hardware, has the features of high accuracy and simple circuit, and in function, parameters can be set both through keystroke and computer serial port. Meanwhile, the voltage and current of power supply are of high accuracy, quick in adjust response and with small output voltage ripple.

异源蛋白在大肠杆菌不能正确折叠表达成包涵体,分子伴侣能在细胞内帮助异源蛋白折叠,改善蛋白的聚集.本研究以大肠杆菌(Escherichia coli) DH5α菌株DNA为模板,利用PCR技术扩增出6个分子伴侣编码基因groEL、groES、dnaK、dnaJ、grpE和clpB,分别将groEL,groES和grpE( I组)插入pCDFDuet-1载体,将dnaK、dnaJ和c1pB( II组)基因插入pRSFDuet-1,构建辅助载体pR-GESP和pC-DJKL,每个重组载体中含有一个人工操纵子,每个基因上游含有T7启动子.SDS-PAGE结果显示,诱导后的重组大肠杆菌上清除了DnaJ外其它5个蛋白明显表达.SDS-PAGE分析了共表达分子伴侣对玉米(Zea mays)四毗咯分子合成的谷氨酸-1-半醛氨基转移酶、尿卟琳原III脱羧酶和西罗叶绿三酸:铁鳌合酶在大肠杆菌的折叠和聚集影响,结果显示,GroEL、GroES和GrpE能防止玉米西罗叶绿三酸:铁鳌合酶在大肠杆菌的聚集,但DnaK、DnaJ和C1pB分子伴侣对该酶则没有作用;GroEL、GroES和GrpE能部分抑制玉米谷氨酸-1-半醛氨基转移酶在大肠杆菌的聚集,但是对改变玉米尿叶啉原III脱羧酶的聚集没有明显作用,这些结果表明,不同分子伴侣组对改善本研究选择的3个玉米蛋白在大肠杆菌可溶性表达的效果有差异.

叶绿体依赖烟酰胺腺嘌呤二核苷酸磷酸(nicotinamide adenine dinucleotide phosphate,NADPH)的硫氧还蛋白还原酶含有还原酶结构域和硫氧还蛋白结构域,每个结构域含有两个催化的Cys残基.利用RT-PCR克隆了编码成熟的依赖NADPH硫氧还蛋白还原酶基因,分别将还原酶结构域以及硫氧还蛋白结构域活性中心两个催化的Cys残基定点突变成Ser残基,将野生型和突变型基因分别插入大肠杆菌(Escherichia coli)表达载体pET-28b,转化至大肠杆菌BL21(DE3),表达的重组蛋白N端含有组氨酸标签.电泳检测显示野生型硫氧还蛋白还原酶的可溶性表达水平受诱导温度和共表达分子伴侣GroEL、GroES和GrpE影响,而蛋白突变体主要表达为包涵体.纯化的重组野生型蛋白SDS-PAGE上显示亚基分子量为52kD,分别以DNTB(6,6′-Dinitro-3,3′-dithiodibenzoic acid)和胰岛素为底物,确定了硫氧还蛋白还原酶两个结构域的催化活性.本研究结果表明,玉米NTRC在大肠杆菌可溶性表达,纯化的重组蛋白具有硫氧还蛋白和硫氧还蛋白还原酶活性.

The ferredoxin(Fd) proteins in plant chloroplasts play important roles in cellular metabolism by delivering reducing equivalents through the [2Fe-2S]cluster in the active site to various essential oxido-reductive pathways.In this study,the chloroplast leading peptides from five Fd proteins of maize share the low homogeneity,whereas the mature Fd proteins deleted the leading peptides are high homogeneous,as displayed by the amino acid sequence alignments.The gene encoding mature maize ferredoxin 1(Fd1) was cloned by RT-PCR using the total RNA from young leaves as the template.The cloned gene was inserted into pQE80 and p28SUMO plasmid,and transformed into Escherchia coli BL21(DE3) respectively.The expressed Fd1 fused with the histidine-tag(His-Fd1) or HisSUMO tag(HisSUMO-Fd1) at N terminus,was purified by Ni-NTA affinity chromatography independently.The recombinant Fd1 protein was obtained by removing the HisSUMO using the specific protease Ulp.SDS-PAGE analysis showed that purified His-Fd1 has a molecular mass of about 12kD.The purified HisSUMO-Fd1 has the absorption peaks at 315,415 and 459 nm identified by UV-visible spectra scanning,and the [2Fe-2S]cluster determined by electron paramagnetic resonance(EPR) experiments.Several proteins from soluble extracts of young maize leaves were bound by the immobilized His-Fd1,as shown by SDS-PAGE analysis.

Using DSP produce a sinusoidal sequence,according to figures oscillator working principle,is to determine th eoscillation frequency signal,and set timers sampling frequency and time intervals.The C5410 DSP using a series of on-chip timer interrupt control registers and registers decided to open up the shield or closing down all the interrupt request.Using assembly language editing stored procedures interrupt service code,interrupt vector table,prepared by the main program with C language and the interrupt service routine.Through two links,graphics CCS final adoption observation tools are sinusoidal output signal.

Glutamate-1-semiadhyde aminotransferase (GSAT) is an enzyme in the upstream biosynthetic pathway of uroporphyrinogen III that is the substrate of uroporphyrinogen III methyltransferase (UPMT), a novel red fluorescent protein. In order to detect the effect of overexpression of GSAT with UPMT on the fluorescent intensity in Escherichia coli, we amplified maize upmt gene by PCR and inserted into the first cistron of pET Duet-1 plasmid to create the vector pETU. The expressed UPMT was fused histidine tag at N terminus. We also amplified E. coli hemL gene encoding GSAT by PCR reaction, eliminated Nco I site within the hemL gene by site-directed mutagenesis and subcloned into pET-51b plasmid. The resultant hemL gene was inserted the second cistron of pETU plasmid to produce the vector pETeGU. The expressed GSAT has the extra Strep-TagII at N terminus. Compared to overexpression upmt gene alone, coexpression both genes did not resulted in the remarkable change in either the amount of the UPMT, as estimated by western blot analysis, or the constitution of red fluorescent materials, as shown by UV/visible light scanning analysis, but increased cellular level of the fluorescent material trimethylpyrrocorphin with the specific absorption at 354 nm. The red fluorescence emitted by the colonies cooverexpressing both enzymes completely disappeared after treated by 2 mmol/L gabaculine, the GSAT inhibitor, suggested that the recombinant GSAT may increase the cellular level of uroporphyrinogen III, and thus enhanced the red fluorescence of the E. coli cells conferred by the recombinant UPMT.

PID(Proportion Integration Differentiation) that the PID control.Parameter tuning of PID control in temperature control system design is the core content.Through the hardware circuit to read the temperature value and to determine the system time base,with 51 monolithic integrated circuit to determine PID algorithm,then use the experimental method to determine the parameters tuning process and methods of the experiment,first set the value of an experience,and then test effect,and gradually make changes to achieve the desired accuracy.

The engineering alternating talent culturation pattern that is based on professional growth becomes the hot and difficutty in the teaching of the professional universities.We have made full use of the resources of our school,made deeply cooperations with FOXON,and innovated an engineering alternating talent culturation pattern that is based on professional growth.

The paper analyses situation of the transport control and management in the mordern city, combines the reality for urban and rural transportion, describes the work rational control system and provides single and low cost design proposal for the hardware circuit of traffic lamp of control for system in the city.