BACKGROUND:Skin photoaging, clinically characterized by wrinkles and hyperpigmentation, accounts for 80% of extrinsic aging. Chronic UV exposure drives this process via oxidative damage. However, its synergistic axis with mitochondrial dysfunction remains mechanistically elusive. This study aims to elucidate the mechanistic link between mitochondrial oxidative stress and UV-induced photoaging, focusing on reactive oxygen species overproduction as a central driver of cellular decline. METHODS:Through integrative analysis of molecular pathways and experimental validation, we investigated mitochondrial dysfunction, ROS accumulation, and UV-induced damage in skin cells. Therapeutic interventions, including mitochondrial-targeted antioxidants (e.g., MitoQ) and protective agents, were tested to assess their efficacy in restoring mitochondrial integrity and mitigating oxidative stress. RESULTS:UV radiation exacerbates mitochondrial dysfunction by inducing ROS overproduction, mtDNA mutations and membrane permeability alterations, creating a vicious cycle that accelerates skin aging. Conversely, mitochondrial oxidative stress amplifies UV-induced damage, promoting collagen degradation and apoptosis. Interventions targeting mitochondrial function, such as MitoQ and mesenchymal stem cell-derived exosomes, significantly reduced ROS levels, preserved membrane potential, and enhanced skin resilience. Notably, PINK1/Parkin-mediated mitophagy and STAT3/p53 pathways were identified as critical regulators of mitochondrial homeostasis during photoaging. CONCLUSION:This study clarifies the bidirectional relationship between mitochondrial stress and photoaging, highlighting ROS as a pivotal mediator. Restoring mitochondrial function via antioxidants or mitophagy enhancers offers actionable strategies to delay skin aging. These findings provide a foundation for novel anti-aging therapies with potential clinical and cosmetic applications.

Background:Bacterial infection and biofilm formation synergistically hinder wound healing by perpetuating inflammation and evading conventional treatments. Monotherapeutic strategies often fail to simultaneously eradicate resilient biofilms and rectify the dysregulated wound microenvironment. To overcome these limitations, we developed a multifunctional and targeted nanoplatform for synergistic antibacterial therapy and immunomodulation. Methods:The smart nanoplatform (CCP-DFO(Fe)) was constructed with a triple-component architecture: a photothermal Cu7S4 core pre-loaded with chlorogenic acid (CGA), enveloped by a thermo-responsive poly(N-vinylcaprolactam) (PVCL) shell, and surface-functionalized with deferoxamine-iron (DFO(Fe)) via amide coupling for active bacterial targeting. Results:The nanoplatform exhibits effective bacterial targeting via DFO(Fe)-mediated siderophore mimicry, enabling preferential accumulation at infection sites. Under NIR irradiation, CCP-DFO(Fe) nanoplatform exhibits efficient photothermal conversion, rapidly elevating the temperature to 44.3 °C within 4 min, which induces the sudden collapse of the PVCL shell from a uniform swollen state to a phase-separated state, leading to shell disruption and consequent exposure of the CGA-loaded Cu7S4 nanoparticles (CSC). Under physiological conditions, the CSC nanoplatform gradually releases Cu2+ and CGA, which, together with the photothermal effect, synergistically exert potent antibacterial activity. As a result, the nanoplatform achieves highly effective bacterial eradication, reducing the survival rates of both E. coli and S. aureus to below 5%, along with pronounced anti-biofilm activity. Beyond its antibacterial activity, the released CGA further exerts antioxidant and anti-inflammatory effects by scavenging reactive oxygen species and promoting macrophage polarization toward the pro-healing M2 phenotype, thereby facilitating inflammation resolution. In an infected rat wound model, CCP-DFO(Fe) combined with NIR irradiation achieved 98.56 ± 1.08% wound closure by day 14, with nearly complete bacterial eradication, while simultaneously promoting angiogenesis and collagen deposition. Conclusion:This integrated nanoplatform combines targeted antibacterial activity, biofilm disruption, and inflammation resolution into a single system, demonstrating significant potential for treating infected and chronic wounds.

Summary:. Cutaneous squamous cell carcinoma (CSCC) of the scalp poses major reconstructive challenges, especially in older patients with poor surgical tolerance. Large postexcisional defects often require complex reconstruction, whereas free flaps and tissue expansion may be unsafe in high-risk individuals. We present a cost-effective, 2-stage approach using xenogeneic acellular dermal matrix (XADM) followed by autologous split-thickness skin grafting. The graft survived completely. Both scalp and donor site healed without infection or wound complications. Frozen sections confirmed negative margins, and final histopathology showed moderately differentiated CSCC without bone invasion. Telephone follow-up indicated durable wound healing without local breakdown until death. The patient died 13 months postoperatively from systemic progression of advanced CSCC rather than surgical complications; formal oncologic surveillance was not performed because of financial constraints. Two-stage XADM-assisted autologous skin grafting is a technically accessible, economical option for large scalp CSCC defects in older, high-risk patients, avoiding free flap morbidity while achieving satisfactory healing and oncologic safety. Prospective studies are warranted. An 82-year-old woman with severe malnutrition (41 kg, 155 cm, body mass index 17.07 kg/m2), cancer-associated cachexia, frailty, and American Society of Anesthesiologists class III presented with an 8 × 9 cm scalp ulcer. Biopsy showed moderately differentiated CSCC. After wide excision with 2.0-cm margins in the subgaleal plane, leaving a 12 × 13 cm defect, the wound was covered with a porcine-derived XADM. Sixteen days later, the matrix was removed, revealing a healthy granulation bed, and an autologous split-thickness skin graft harvested from the dorsal back was applied.

BACKGROUND:Dermal fillings based on hyaluronic acid have been widely used in facial rejuvenation. A number of studies have reported the beneficial effect of hyaluronidase in saving the complications of hyaluronic acid. However, the effects of hyaluronidase on dermal matrix, skin structure, and fibroblast function are unknown. METHOD:In this study, hyaluronic acid and hyaluronidase were injected into rat skin, skin samples were collected at multiple time points, and the changes of skin structure, fibroblast function, and extracellular matrix gene and protein expression were detected. RESULT:The results in vivo showed that hyaluronidase can effectively degrade exogenously injected hyaluronic acid in rats without causing significant changes in dermal matrix and skin structure. The results in vitro showed that hyaluronidase had no significant effect on proliferation, apoptosis, and collagen synthesis of fibroblasts. CONCLUSION:This study provided a theoretical basis for the clinical application of hyaluronic acid and hyaluronidase. In vivo and in vitro experiments confirmed that hyaluronidase can degrade exogenous hyaluronic acid without damaging the skin structure, dermal matrix, and fibroblast function.

Introduction: Despite advances in contemporary burn care, the discriminative performance and clinical applicability of traditional prognostic scoring systems may be increasingly limited. Machine learning models have been applied to predict burn mortality; however, their overall predictive performance and methodological quality have not been systematically evaluated. Methods: This systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Discriminative performance was assessed using the C-statistic. Pooled C-statistics and corresponding 95% credible intervals for machine learning models were estimated within a Bayesian framework. Subgroup and sensitivity analyses were performed according to model type, including random forest, support vector machine, and logistic regression, as well as the degree of class imbalance. Risk of bias and publication bias were also assessed. Results: Twelve studies using diverse algorithms and data sources were included. The pooled C-statistic for the best-performing models was 0.96 (95% CrI: 0.93-0.98; 95% PI: 0.84-1.00), indicating excellent overall discrimination. Ensemble and more complex algorithms, such as random forest and support vector machine, outperformed single decision- tree models. Logistic regression also demonstrated stable performance. After excluding datasets with severe class imbalance, the overall findings remained largely unchanged. Funnel plot assessment and Egger’s test did not indicate clear evidence of publication bias. Discussion: The findings indicate that ML models have considerable potential for predicting mortality in patients with burns. However, methodological limitations, including a high risk of bias, limited external validation, and insufficient model interpretability, may restrict their current clinical applicability. Conclusion: ML models demonstrate excellent discriminative performance for predicting mortality in patients with burns. Future high-quality multicenter studies with rigorous external validation, transparent reporting, and improved model interpretability are needed before these models can be routinely implemented in clinical practice. Registration: The protocol for this study was registered with PROSPERO (registration number: CRD420251153818).

A substantial obstacle in the treatment of burn patients is the management of burn wound infection (BWI). The objective of this investigation was to assess the antimicrobial susceptibility test results and distribution characteristics of microorganisms isolated from the burn wounds of patients affected by the factory gunpowder and pyrotechnic explosions. This tertiary burn center conducted a 5-year retrospective observational study of the clinical and laboratory characteristics of pathogenic microorganisms through drug susceptibility tests, species distribution, and timing of wound infections. Two groups of patients were compared: patients with burn wound infection (BWI) versus patients without burn wound infection (Non-BWI). The study enrolled 313 individuals (median age: 47 years), of whom 286 (91.37

Skin wound infections are common and clinically challenging. Conventional antibiotic therapies are increasingly ineffective because of escalating bacterial resistance, highlighting the urgent need for alternative treatment strategies. Antibacterial hydrogels, multifunctional polymeric materials that integrate moisturizing, drug delivery, controlled release, and wound-healing properties, have emerged as highly promising candidates for managing infected wounds. Based on their underlying antimicrobial mechanisms, these systems can be broadly classified into three main categories: chemical, physical, and biological antibacterial hydrogels, which achieve bactericidal efficacy through drug release, physical disruption, or modulation of the host microenvironment and immune responses, respectively. Of tremendous significance is the advent of stimuli-responsive intelligent hydrogels, which provides new opportunities for achieving precise and efficient antibacterial therapy. This review systematically summarizes the material selection, design strategies, and representative advances in antibacterial hydrogels, with particular emphasis on their core mechanisms, strengths, and limitations, aiming to offer theoretical foundations and research perspectives for the rational optimization and clinical translation of next-generation antibacterial hydrogels.

ABSTRACT Objective Exosomes (Exos) from adipose derived stem cells (ADSCs) can delay skin photoaging, but their effects on reactive oxygen species (ROS) remains unclear. This study aimed to investigate the relationship between adipose derived stem cell exosomes (ADSCs‐Exos) in anti‐photoaging of skin and glutathione (GSH)/ ROS expression in human fibroblasts. Methods A skin photoaging model was established by irradiating human fibroblasts with ultraviolet B (UVB) light in vitro. Next, exosomes from ADSCs were isolated for treating the photoaged fibroblasts. Afterwards, the alterations in photoaged fibroblasts were analyzed by a series of assays including senescence‐associated β‐galactosidase (SA‐β‐Gal) staining, p16 expression, ROS staining, and GSH content. Results After a human fibroblast photoaging model was subjected to ADSCs‐Exos treatment, we found that the high concentration exosome group had the highest GSH content. Cellular staining showed that levels of SA‐β‐Gal, p16, and ROS of the high concentration‐treated group were lower than other groups. Conclusions ADSCs‐Exos can protect skin fibroblasts from photoaging via increasing the ratio of GSH/ROS.

Mitochondrial DNA (mtDNA) deletion and oxidative stress are key contributors to skin photoaging. Mitophagy helps mitigate oxidative stress. Human adipose-derived stem cell exosomes (hADSC-Exos) have been shown to counteract skin photoaging. This study aimed to explore the role and mechanism of hADSC-Exos in addressing skin photoaging. hADSC-Exos were isolated, and their surface markers were identified. Human dermal fibroblasts (HDFs) and nude mice were exposed to ultraviolet-B (UVB) irradiation, and treated with hADSC-Exos. Oxidative stress and photoaging were assessed through SA-β-gal staining, p21 expression, mtDNA deletion, reactive oxygen species (ROS) levels, and histological analysis. The PINK1, Parkin, LC3b, and p62 protein levels were measured to evaluate mitophagy. The PINK1 small-interfering RNA (siPINK1) was then used in HDFs to investigate the role of hADSC-Exos in mitophagy. In UVB-exposed HDFs and nude mice, the number of SA-β-gal-positive cells, along with levels of p21, ROS, and mtDNA deletion, were significantly increased, but these effects were reduced by hADSC-Exos. Moreover, hADSC-Exos treatment significantly elevated PINK1 and Parkin levels, as well as the LC3bII/I ratio, while reducing p62 expression. In photoaged HDFs treated with hADSC-Exos, PINK1 knockout using siRNA decreased the LC3bII/I ratio and levels of PINK1 and Parkin, while increasing p62, ROS, and mtDNA deletion compared to the negative control (NC) group. hADSC-Exos can mitigate skin photoaging by promoting PINK1/Parkin-mediated mitophagy, thereby reducing mtDNA deletion and oxidative stress.

Chronic diabetic wounds are notoriously difficult to heal due to the self-perpetuating cycle of persistent inflammation and oxidative stress, while current therapies are limited by single-action mechanisms and inefficient drug delivery. This study developed a reactive oxygen species (ROS)/pH dual-responsive hydrophilicity switching intelligent hydrogel (GC-HA@ZIF-8@Cur) by integrating a zeolitic imidazolate framework-8 (ZIF-8) with a dynamically crosslinked hydrogel for synergistic therapy. The system employs inflammation-targeting hyaluronic acid (HA)-modified ZIF-8 nanoparticles (HA@ZIF-8@Cur) to encapsulate curcumin (Cur), which are embedded into a ROS-responsive hydrogel matrix formed by ultraviolet-initiated polymerization of methacrylated gelatin and lipoic acid-grafted chitosan. In the ROS microenvironment of diabetic wounds, oxidation of thioether bonds in the hydrogel to sulfoxide bonds enhanced the hydrophilicity, while acidic conditions induced pH-responsive dissociation of ZIF-8 to cascade-release Cur and Zn2+. Experiments demonstrated that GC-HA@ZIF-8@Cur hydrogel reshapes the immune microenvironment by downregulating pro-inflammatory factors (interleukin [IL]-6, tumor necrosis factor [TNF]-α), polarizing macrophages toward the M2 phenotype, and upregulating IL-10, eliminating vascular generation disorders. Additionally, Zn2+ promotes vascular endothelial growth factor (VEGF) expression, accelerating angiogenesis. This dual-responsive system achieves spatiotemporally precise drug release, concurrently addressing inflammation, oxidative stress, and vascular regeneration barriers, significantly improving diabetic wound healing efficiency (96.372 ± 0.779% wound closure at day 14). It provides a novel multi-targeted co-delivery strategy for chronic wound therapy.

Autologous diced cartilage, while biocompatible and easy to shape, is limited in clinical application due to its high adsorption rate and challenges in establishing timely and effective neovascularization postsurgery. In this study, the authors produced SVF cell sheets from adipose-derived stromal vascular fraction (SVF) through enzymatic digestion, employing a temperature-sensitive culture system. Our in vivo and in vitro experiments validated that SVF cell sheets, when wrapped around granular cartilage, exhibited a notable promotion of cartilage regeneration and mitigated granular cartilage adsorption in a rabbit diced cartilage graft model. Our findings demonstrate that SVF cell sheets facilitated effective neovascularization and timely cartilage block formation by secreting VEGF and Ang-1 while also suppressing the expression of pyroptotic proteins like NLRP3, Caspase1, and GSDMD. As a biofilm, derived from a multicellular source, SVF cell sheets can replace perichondrium and promote the expression of proangiogenic growth factors Ang-1 and VEGF, thereby promoting local microvascular regeneration, reducing chondrocyte pyroptosis, and promoting the formation of cartilage blocks. This strategy provides a potential new method for autologous cartilage grafting, which will help solve the dilemma of limited sources of cartilage tissue in clinical practice and provide natural autologous cartilage filling materials for the treatment of craniofacial defects.

Extensive skin injuries often lead to chronic wound healing, scar formation, and elevated mortality rates. Existing treatment options, including autologous, allogeneic, and xenogeneic skin grafts, are constrained by donor scarcity, low graft survival rates, scarring, flap edema, and the inability to regenerate skin appendages. Consequently, the development of tissue-engineered skin substitutes comprising scaffolds, cells, and bioactive factors has emerged as a promising approach for repairing extensive skin defects. Herein, we optimized the electrospinning technique to construct a three-dimensional polycaprolactone (PCL) nanofiber scaffold with a thickness of 5 μm ± 0.7 μm and a porosity of 85%. Dopamine self-polymerization was employed to form a polydopamine (PDA) coating, which was subsequently combined with type I collagen (COL) to produce a polycaprolactone-polydopamine-collagen (PPC) scaffold. The PPC scaffold demonstrated significantly enhanced mechanical strength and hydrophilicity, along with excellent biocompatibility. The biocompatibility of the PPC nanofiber scaffold was markedly improved, effectively promoting the migration, adhesion, and proliferation of keratinocytes (KCs). Co-culturing autologous KCs with the PPC scaffold to construct an epidermal membrane graft significantly accelerated wound healing, resulting in a more complete neo-skin tissue structure and improved wound healing quality. Moreover, qPCR and Western blot analyses were employed to assess key protein and gene expression levels in the Wnt signaling pathway, revealing that our epidermal cell membrane could activate the Wnt signaling pathway, thereby promoting wound healing. This tissue-engineered epidermis offers a promising alternative to traditional skin grafting methods and may address the limitations associated with extensive skin injuries.

Psychological disorders play a crucial yet often overlooked role in medical aesthetic disputes. Patients with underlying psychological conditions may have unrealistic expectations, leading to dissatisfaction and legal conflicts. This review aims to explore the impact of psychological disorders on medical aesthetic disputes, analyze their prevalence, and discuss strategies to mitigate associated risks. A comprehensive review of existing literature was conducted, focusing on the relationship between psychological disorders—such as body dysmorphic disorder (BDD), anxiety, and depression—and medical aesthetic disputes. Key factors influencing patient satisfaction and dispute resolution were examined. Psychological disorders are significantly associated with higher dissatisfaction rates and increased risk of conflicts in aesthetic medicine. Inadequate psychological screening and poor communication between practitioners and patients contribute to these disputes. Implementing thorough psychological assessments and improving patient selection criteria can help reduce conflict and enhance treatment outcomes. Understanding and addressing psychological disorders in aesthetic patients is essential for reducing disputes and improving patient satisfaction. Integrating mental health evaluations into aesthetic practice, along with better communication strategies, can help prevent conflicts and optimize clinical outcomes. Collaboration between aesthetic professionals and mental health specialists is recommended to ensure comprehensive patient care. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Pyroptosis, a programmed cell death marked by lytic and inflammatory characteristics, plays a crucial role in non‑infectious inflammation‑related diseases but can lead to detrimental outcomes when dysregulated. Stem cells have emerged as key players in modulating pyroptosis through paracrine signaling, offering a novel avenue for tissue repair and regeneration. The present review delved into previous studies elucidating the intricate interplay between stem cells and pyroptosis, emphasizing the potential of stem cell‑based therapies in regulating pyroptotic pathways. The exploration of this dynamic interaction holds promise for developing strategies to harness stem cells for effective tissue repair and regeneration in the context of inflammation‑related diseases.

Purpose This study aimed to evaluate the effectiveness of integrating artificial intelligence (AI) with problem-based learning (PBL) and case-based learning (CBL) in a dermatology cosmetology course for clinical medical interns. Materials & Methods This prospective randomized controlled trial involved 70 clinical interns rotating in the medical cosmetology department. Participants were randomly assigned to two groups and followed the same curriculum over 8 weeks. The experimental group (n = 40) used a teaching method combining AI with PBL and CBL, while the control group (n = 30) followed the traditional PBL and CBL approach. Assessments included theoretical exams, case analysis, and anonymous feedback surveys to evaluate teaching quality. Results All participants completed the examinations and questionnaires. The average theoretical test scores and case analysis test scores of the experimental group were higher than those of the control group (P < 0.001). The indicators of the experimental group’s feedback were better than those of the control group, such that the improvement of learning interest and motivation, the improvement of understanding of diseases and knowledge, the improvement of independent learning ability, the improvement of clinical thinking and summary ability, as well as a more positive classroom atmosphere and more helpful for future clinical work (P < 0.05). Conclusion Compared to the PBL combined CBL teaching method, the teaching mode combining AI with PBL and CBL teaching method showed a higher efficacy. The learning model effectively improved students’ outcomes and satisfaction, which helped students narrow the gap between theoretical knowledge and clinical practical application.

Microtia has severe physical and psychological impacts on patients, and auricular reconstruction offers improved esthetics and function, alleviating psychological issues. Microtia is a congenital disease caused by a multifactorial interaction of environmental and genetic factors, with complex clinical manifestations. Classification assessment aids in determining treatment strategies. Auricular reconstruction is the primary treatment for severe microtia, focusing on the selection of auricular scaffold materials, the construction of auricular morphology, and skin and soft tissue scaffold coverage. Autologous rib cartilage and synthetic materials are both used as scaffold materials for auricular reconstruction, each with advantages and disadvantages. Methods for achieving skin and soft tissue scaffold coverage have been developed to include nonexpansion and expansion techniques. In recent years, the application of digital auxiliary technology such as finite element analysis has helped optimize surgical outcomes and reduce complications. Tissue-engineered cartilage scaffolds and 3-dimensional bioprinting technology have rapidly advanced in the field of ear reconstruction. This article discusses the prevalence and classification of microtia, the selection of auricular scaffolds, the evolution of surgical methods, and the current applications of digital auxiliary technology in ear reconstruction, with the aim of providing clinical physicians with a reference for individualized ear reconstruction surgery. The focus of this work is on the current applications and challenges of tissue engineering and 3-dimensional bioprinting technology in the field of ear reconstruction, as well as future prospects.

Background: Burns represent important global health problems. Whereas many studies are limited by the difficulties in estimating the burden of burns and instead focus on the causes of burns, such as fire, heat, and hot substances. Therefore, a complete assessment of the burden of all injuries leading to burns is essential to developing reasonable global intervention strategies. Methods: Data on three classes of burns, including " < 20 % total burned surface area without lower airway burns" (Moderate injury), " > =20 % total burned surface area or > = 10 % burned surface area if head/neck or hands/wrist involved w/o lower airway burns" (Major injury), "Lower airway burns" (Inhalation injury) were collected from the Global Burden of Disease 2019 database. Age -standardized incidence rates (ASR -I) and Years Lived with Disability (ASR-YLDs) for burns has been standardized by removing the influence of population size and age structure. They were extracted and stratified by cause, year, sex, age, socio-demographic index, country, and territory. Results: In terms of ASR -I and ASR-YLDs, burns showed a significant decrease from 1990 to 2019, especially for moderate and major injury. In 2019, the burden of moderate injury was positively correlated with socio-demographic index while major injury was negatively correlated (P < 0.05). We found no correlation between socio-demographic index and the burden for inhalation injury (P > 0.05). Fire, heat, and hot substances were the most important cause of burns except for inhalation injury. The most common association with inhalation injury was falls, which were also a major cause of moderate and major injury. Conclusions: The Global Burden of Disease 2019 database data can be used to guide the allocation of resources to reduce ASR -I and ASR-YLDs of different burn classes. (c) 2023 Elsevier Ltd and ISBI. All rights reserved.

OBJECTIVE:To investigate the role of miR-30a-5p on the proliferation and apoptosis of hair follicle stem cells (HFSCs) and whether the Wnt/β-catenin signaling pathway is involved. METHODS:HFSCs derived from the vibrissa of mammary rats were obtained by enzymatic digestion, and subsequently the obtained HFSCs were treated with Lipofectamine 2000 cell transfection and divided into normal cell culture group (control), miR-30a-5p overexpression group (miR-30a-5p mimic), miR-30a-5p empty vector group (miR-NC), miR-30a-5p inhibitor group (in-miR-30a-5p), and in-miR-30a-5p empty vector group (in-miR-NC). After transfection, the cell proliferation and apoptosis rates were examined separately. In addition, the mRNA expression of β-catenin, proliferating cell nuclear antigen (PCNA) and apoptosis-related genes (Bax and Bcl-2) were examined. RESULTS:The results of cell proliferation ability showed that in-miR-30a-5p group promoted cell proliferation of HFSCs relative to other groups, along with significant upregulation of gene levels of PCNA. Apoptosis analysis indicated that apoptosis rate was reduced in the in-miR-30a-5p group, and the expression of Bax was suppressed, while that of Bcl-2 was promoted. Wnt/β-catenin signaling pathway investigation revealed a significant increase in the levels of β-catenin in HFSCs in the in-miR-30a-5p group. CONCLUSION:Downregulation of miR-30a-5p levels inhibited HFSCs apoptosis and simultaneously promoted proliferation, furthermore, the increased expression of β-catenin indirectly confirmed the activation of the Wnt/β-catenin signaling pathway.

Cancer is a serious disease that threatens human health. Radiation, chemotherapy, and surgical excision are the main strategies used to treat tumors. However, important targets and vital genes have gradually come to light. TIMPs, inhibitors of the matrix metalloproteinase, were related to tumor progression. It is still uncertain, nevertheless, how they affect the progression and prognosis of pan-cancer. Therefore, the profiles of TIMP gene family expression in various cancers were evaluated via The Cancer Genome Atlas (TCGA) database. The prognostic value of the TIMP gene family was investigated by K-M plotter and Cox regression analysis. Additionally, we explored the association among TIMP gene family expression level, immune cell infiltration, immune-related genes, and linked pathways. We found in several malignancies, there were notable differences in the TIMP gene family expression levels between tumor and normal tissues. Meanwhile, a prognosis study revealed clear connections between the the TIMP gene family expression and the clinical prognosis of cancer patients in many cancers. Additionally, the TIMP gene family expression was associated with the infiltration of immune cells, especially macrophages. Thus, we proposed the TIMP gene family could be addressed as novel biomarkers for determining prospective prognostic values and potential therapeutic targets for cancer patients.