In order to solve the problems of low accuracy,long time for error testing and low automation when peple use traditional instruments to test the error of degaussing equipment.In this paper,the degaussing system error sources and characteristics of degaussing equipment are discussed.It analyzes the feasibility of a common error handling method such as the least squares method and practical filter analysis.Meanwhile it also researches the test methords about degaussing equipment static characteristics,dynamic characteristics,transient characteristics and normality characteri-stics.This paper studies the algorithms of the system self-correction technology,gives the three stages of data calibration method to eliminate the systematic errors,devises the differential amplifier circuit to suppress the zero-drift phenomenon,Based on virtual instrument technology and Labview platform,according to the proposed test methods and analytical methods,it gives a successful implementation of a degaussing power error testing system.

Actinobacillus pleuropneumoniae(App),Haemophilus parasuis(Hps),Bordetella bronchiseptica(Bb),Toxigenic Pasteurella multocida(T+Pm) and Mycoplasma hyopneumoniae(Mhp) are five kinds of the most common pathogenic bacteria which can cause a series of respiratory tract diseases in swine.Five pairs of specific oligonucleotide primers were designed according to the DNA sequences of App apxIV,Hps 16S rRNA,Bb fla,toxigenic T+Pm toxA and Mhp ldh reported in NCBI website.A multiple PCR detection method was established according to improved systems and programmes on the basis of five single PCR methods.No cross reaction was detected by the multiplex PCR method between these five bacteria templates and other common bacteria templates.The sensitivity of multiple PCR was lower than 160 pg genome template.Using this multiple PCR method,bacteria could be detected from lung tissues of challenged mice with App,Hps,Bb and T+Pm respectively after being cultured for 8 h in Tryptic Soy Broth(TSB) supplemented with 5% calf serum and NAD+ factor.Simultaneous detection of pathogens in 269 clinical samples from the central area of China revealed the universal existence of App,Hps,Bb,T+Pm and Mhp.In conclusion,the multiple PCR assay showed excellent specificity,sensitivity and reduplication and could detect five pathogenic bacteria of respiratory tract in swine.We expected that this multiple PCR assay could be useful extensively for epidemiological survey and clinical differential diagnosis of five common respiratory diseases in swine.

Emergency plan inquiry and management is a part of Heilongjiang provincial traffic emergency guarantee system.This system can enhance the efficiency of Heilongjiang provincial traffic emergency rescue,reduce the degree of traffic accident damage and make sure the safe running of traffic.

为了测定伪狂犬病毒中和抗体与gB抗体之间的相关性,本试验用216份伪狂犬病毒中和抗体阳性血清,对每一份样本作4种不同的稀释(1∶1、1∶20、1∶40、1∶80),用Idexx公司的gB-ELISA试剂盒检测。试验结果表明:216份中和抗体阳性血清样品中,有7份gB抗体阴性,阳性检出率为97.8%(209/216);随着中和抗体效价的增高gB-ELISA OD650值降低;gB抗体阻断率随中和抗体效价的增高而增加;对同一份血清样本作1∶1、1∶20、1∶40、1∶80稀释,测定结果发现,随着稀释度的增加,gB抗体阻断率逐渐降低,而gB-ELISAOD650值逐渐升高。

This paper described a multiplex RT-PCR to rapidly diagnose the co-infection in samples from pig with reproductive failure symptoms.Three sets of primers were designed,respectively,targeting to the conserved sequences in E2 gene of classical swine fever virus(CSFV),a domain encompassing partial ORF6,ORF7 and 3-terminus UTR of porcine reproductive and respiratory syndrome virus(PRRSV) and E gene of Japanese encephalitis virus(JEV).The expected sizes of RT-PCR amplicons were 288 bp for CSFV,430 bp for PRRSV and 1 015 bp for JEV,respectively.RNA was extracted from pure culture of the viruses,followed by the synthesis of cDNA that was used as templates for method development.Through optimization by using three sets primers simultaneously,the detection limit of RNA template in the RT-PCR was 0.27 ng for CSFV,0.049 ng for PRRSV and 0.067 for JEV.No false positive results were produced in the case of transmissible gastroenteritis virus(TGEV),porcine parvovirus(PPV),pseudorabies virus(PrV) and porcine circovirus type 2(PCV-2).There were 174 samples,including sera,fetuses and lung tissues which were collected from the pigs that mainly presented high fever,were subjected to our in-house RT-PCR.PRRSV was detected in these samples with higher detection rate than CSFV and JEV.Among 174 clinical samples,PRRSV-positive percentage was 30.4,CSFV-positive percentage was 4.6,JEV-positive percentage was 1.7,co-infection of CSFV and PRRSV percentage was 1.7.In order to confirm the reliability of the multiplex PCR,we selected PCR products of CSFV-positive JEV-postive and PRRSV-positive respectively that detected clinical samples by multiplex PCR.After sequences analysis and BLAST,it is showed that homologies between PCR products of CSFV,PRRSV,JEV and target genes sequences were 92%,90% and 89%,respectively.Those results proved that multiplex RT-PCR have Applied value in practices.

In view of traffic emergency on the road in Heilongjiang Province,combined with emergency operation's various links characteristics,the relevant contents in traffic emergency were analyzed in this paper,which included prevention,the matter rescued in anticipation,afterwards processes and so on.The research categories of road traffic emergency safeguard system of Heilongjiang Province were determined,and the Heilongjiang Province Road Traffic Emergency Safeguard System was initially investigated.

从公路自动收费系统入手,分析了作为自动收费系统关键技术之一的车牌自动识别技术。并以其工作过程为主线,以每个工作环节中常用的方法为例,详细的介绍了基于图像处理的车牌识别的工作原理。