The problem reviewed of building intelligent decision support systems for classification and prediction of the functional state of complex systems in the article. To predict the state of complex systems, hybrid decision modules with virtual flows are proposed, which reflect the hidden system connections between real and virtual data. The vector of informative features at the input of the hybrid decision module consists of two subsectors, the first of which corresponds to real flows, and the second - to virtual flows. Simulation modeling of classification processes using latent variables was performed, which allowed to evaluate the effect on the quality of classification of artificially introduced virtual flows. The structure of a neural network model with virtual recurrent-type streams is developed. The structure consists of N consecutively included neural network approximants. The outputs of the previous approximators are combined with the vector of in-formative attributes of the subsequent approximators, which allows forming virtual flows of different dimensions. A method is developed for the formation of non-linear models of virtual flows, characterized by the use of the GMDH-simulation method to obtain models of the influence of real flows on virtual flows, learned through nonlinear adalines. The method makes it possible to form a subvector of latent variables of unlimited dimension. Non-linear models of virtual flows are formed through a method based on the use of GMDH modeling. The method makes it possible to obtain neural network structures built on the basis of GMDH models and nonlinear adalines, which make it possible to form a subvector of latent variables of unlimited dimensionality.

The severity of complications and a large percentage of disability related to the occurrence cold injury lead to medical, social and economic problems. Considering the contact of cold injury as a variety of acute ischemia with the subsequent formation of early and late postischemic disorders as causes of disorders of blood circulation in the affected segments can be considered a disorder of the oxidant-antioxidant status and cytokine, systemic inflammation, reperfusion syndrome, the development of endothelial dysfunction, which ultimately leads to multiple organ failure. In the course of the study when assessing the microcirculatory changes caused by the simulation of frostbite, the most pronounced effect of the combined treatment of Serotonin adipinate 4.5 mg/kg and opioid peptide DSLET 10 μg/kg compared with monotherapy with the studied drugs was estimated. The most effective correction of indices of oxidantantioxidant protection observed with the combination of necrectomy with a combination of Serotonin adipinate and opioid peptide DSLET, which was confirmed by approximating the indicators of malondialdehyde, superoxide dismutase, finite stable metabolites of nitrogen oxide, total antioxidant activity and catalase to the values of intact animals on the 14th day of the experiment and reducing the level of proinflammatory cytokines (interleukin-6, tumor necrosis factor-alpha) in 2 times (p < 0.05) compared to the Control group. The combined use of Serotonin adipinate 4.5 mg/kg and opioid peptide DSLET of 10 μg/kg in combination with active surgical tactics (necrectomy) helps reduce the severity and prevalence of alternative changes, accelerate the formation of granulation shaft, a more intense development of reparative processes in the simulation of cold injury compared with the monotherapy. The combined use of investigational drugs in combination with active surgical tactics led to the maximum enhancement of the survival of the animals with contact frostbite in the experiment.

The problem of managing patients with deep necrosis of the skin and soft tissue remains relevant. Selecting necrectomy volume, timing of its implementation, the boundaries of performing surgical incisions are still not fully resolved. Necrosectomy against the background of the DSLET combination (10 mg / kg) and serotonin adipate (4.5 mg / kg) resulted in a decrease in the severity and prevalence of alternative changes in simulating deep necrosis of the skin and soft tissues. On the 3d day the granulation tissue was formed in wound edges. By the end of the experiment the whole damage area was formed by connective tissue with the newly produced dense collagen fibers indicating the maturation of connective tissue in the generating scar. The suggested method results in a maximum decrease in mortality to 16.6% and also reduces the lethality of animals by 10.9% as compared to the experimental group without necrectomy.

The experiments on rats studied the electrical activity (EA) of various parts of stomach and duodenum under simulation of intra-abdominal hypertension (IAH). 24 hours after modeling IAH it was found that the average values of the EA amplitude in the pyloric part of stomach decreased by 27% (p <0.05) and the frequency of EA increased by 124.9% (р<0.01) in stomach, and by 63.2 % (р<0.01) in the initial part of duodenum. The correlation of EA between all the departments of gastroduodenal complex increased. It was found that the introduction of serotonin adipate eliminates the disturbances of EA in the pyloroduodenal area and prevents the development of systemic gastroduodenal disorders.

Active surgical tactics (necrectomy) with the combination of serotonin adipate 4.5 mg/kg and DSLET 10 mсg/kg reduces the severity and extent of alterations; contributes to faster formation of granulation bank, and more intensive reparative processes in simulating cold injury. The most effective correction of contact frostbite was detected in conjunction of necrectomy and combinations of serotonin adipate and DSLET, as evidenced by the correction of such indicators as malondialdehyde, superoxide dismutase, the final stable nitric oxide metabolites, total antioxidant activity and catalase up to the values of intact animals on the 14th day of the experiment and halved levels of proinflammatory cytokines (IL-6, TNF-α) (p < 0.05) compared with the control group.

В двух сериях экспериментов на крысах исследована электрическая активность (ЭА) различных отделов желудка и двенадцатиперстной кишки в условиях моделирования интраабдоминальной гипертензии (ИАГ). Через 24 часа после моделирования ИАГ обнаружено снижение средних значений амплитуды ЭА в пилорическом отделе желудка на 27 % (р < 0,05) и увеличение скоррелированности амплитудных характеристик ЭА между всеми отделами гастродуоденального комплекса (ГДК). Через 48 часов средние значения амплитуды ЭА снижались в пилорическом отделе желудка на 39,7 % (р < 0,01) и на 30,3 % (р < 0,01) в его фундальном отделе, а скоррелированность амплитудных значений ЭА между этими отделами желудка еще более возрастала (r = 0,904 ± 0,02, р < 0,01). Картину изменений функциональных взаимоотношений между компонентами ГДК можно трактовать как явление утраты его функциональной гетерогенности на фоне угнетения электрической активности гладких мышц желудка вследствие нарастающей интраабдоминальной гипертензии.

Nowadays the cold injury remains the actual surgical problem despite of the long-term studies of the mechanisms of its pathogenesis and the emergence of new advanced treatment technologies. The active surgical approach of frostbite’s treatment in conjunction with the combined administration of DSLET (10 mcg/kg a day) and Serotonin adipinate (4,5 mg/kg a day) resulted in a decrease in the severity and prevalence of alterations due to the contact frostbite modeling. During microscopic examination it was shown the necrotic mass are absent on day 3, also there are small inflammatory cell infiltrates. The granulation tissue is formed in wound edges. By the 7th day the granulation layer thickens owing to the dense connective tissue fibers are accumulated. At the end of our experiment the whole damage area is filled by connective tissue, this fact suggests that the newly formed collagen fibers mature in the forming cicatrix. The efficiency of contact frostbite correction by the combined administration of DSLET (10 mcg/kg a day) and Serotonin adipinate (4,5 mg/kg a day) in conjunction with the active surgical approach is confirmed by the 2-fold reduction of pro-inflammatory cytokines such as IL-6, TNF-α (p

Classification of functional states and assessment of psychoemotional stress and fatigue levels based on hybrid fuzzy models is discussed in terms of the risk of diseases.

The paper describes the mathematical model of acute pancreatitis prediction based on information received with traditional medical methods (interview, physical examination, lab and instrumental tests), by electrical resistance of connected with disease bioactive points and on the content of micronutrients in human hair.

An algorithm for allocation of slow waves and respiration rhythms from electrocardiosignal using quasi-periodic signals of living systems is described. The algorithm is based on detection of amplitude and morphology of cardiosignals. The algorithm allows slow and respiratory waves of cardiosignal to be localized using window Fourier transform.

Relevance Increased knowledge of the pathogenesis of frostbite and new pharmacological agents make it possible to improve the treatment results of local cold injury. This can be achieved, in particular, through the introduction of drug combination - the selective agonist of the delta-opioid receptors (DSLET) and the serotonergic agent (serotonin adipinate) which possesses analgesic, immunomodulatory, antioxidant and anti-inflammatory activity. The purpose of the study To investigate the effect of combined pharmacological treatment by DSLET and serotonin adipinate in experimental local frostbite. Materials and methods The study was conducted on 200 male Wistar rats. The local frostbite was inflicted with a preliminary cooled in liquid nitrogen metal plummet size 3.5-3.0 cm pressing against the shaved skin of the back during 3 min. Alongside with the standard antibiotic therapy (control) animals of the experimental groups were treated DSLET (10 mkg/kg intra-peritoneally (IP), serotonin adipinate (4.5 mg/kg IP) or its combination in indicated doses. Using the method of laser Doppler flowmetry the level of microcirculation was assessed as well as the biochemical changes in serum (malondialdehyde (MDA), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), superoxide dismutase (SOD), catalase) in all animals at 1, 3, 7 and 14 days after cold injury. Results and their discussion The model of local frostbite in control group resulted in reduction of microcirculation in necrosis boundary area at 1, 3, 7 and 14 days compared to the intact animals. The local frostbite injury induced oxidative stress and inflammation that were confirmed by typical dynamics of serum biochemical markers of non-treated rats while the significant increase the concentration of MDA, IL-6, TNF-α and decrease SOD activity were recognized. Also compensatory enhancement of catalase activity – one of the major antioxidant enzymes was observed. As well additional administration of drug combination DSLET (10 mkg/kg) with serotonin adipinate (4.5 mg/kg) to antibacterial treatment by cefotaxime in dose 0.085 g/kg increased the microcirculation by 27.2% compared to the non-treated group (р<0.05) at day 14 after damage due to frostbite injury in rats. A curative beneficial effect caused by combined pharmacological treatment of local frostbite was also confirmed by decrease of MDA concentration and pro-inflammatory cytokines IL-6 level at 1, 3, 5 and 7 days and by enhance of catalase and SOD activities at day 7 and 14 following experimentally induced frostbite. Conclusion Compared to monotherapy, combined usage of DSLET and serotonin adipinate provides a better pharmacological treatment efficiency in experimental model of local frostbite.