近年,全球消化道肿瘤的发病人数及死亡人数逐年递增,结直肠癌占消化道肿瘤比例最大,其重要的死亡原因是术后的转移及复发.结直肠癌肺转移的治疗方式逐渐多元化、个体化,多种治疗方式相结合,明显提高了患者的生存时间.

In order to solve the problem of the application of data acquisition in the new nanometer drug delivery system of microscope, a research of antigallbladder carcinoma activity analysis was proposed. Gallbladder carcinoma (GBC) is a common malignant tumor in biliary tract diseases. Due to the lack of specific clinical manifestations in the early stage, GBC has the shortcomings of the hidden onset, the difficult diagnosis, and the high misdiagnosis rate. GBC ranks in the top position among the most common tumors of the digestive system worldwide. The preoperative diagnosis rate is low, and the incidence of accidental gallbladder carcinoma is gradually increasing. Domestic gallbladder carcinoma related to cholecystectomy is not sensitive to radiotherapy and chemotherapy. Surgical resection is still the only effective method for the treatment of accidental gallbladder carcinoma.

目的:研究Th22细胞及效应因子IL-22与IL-9在肺癌患者外周血中的表达及相关性分析.方法:收取2018-11 ～2019-05在我院住院确诊为肺癌患者35例为实验组;对照组为来自于同一时期门诊体检的35例健康人群;用流式细胞术检测外周血中Th22细胞(CD4+ IL-22+)的比例,用酶联免疫吸附(ELISA)法检测血清中IL-22、IL-9的表达水平.结果:在肺癌患者中,Th22细胞、IL-22、IL-9因子的表达均高于对照组(P＜0.01),差异有显著性,并且Th22细胞与效应因子IL-22在实验组表达水平呈正相关,IL-22因子、IL-9因子的表达水平呈正相关.结论:肺癌患者外周血中Th22、IL-22、IL-9的表达频率明显升高,与肺癌的发生发展相关,有望为肺癌的研究增添新的思路.

Background: To classify triple-negative breast cancer (TNBC) immunotyping using the public database, analyze the differences between subtypes in terms of clinical characteristics and explore the role and clinical significance of immune subtypes in TNBC immunotherapy. Methods: We downloaded TNBC data from the cBioPortal and GEO databases. The immune genes were grouped to obtain immune gene modules and annotate their biological functions. Log-rank tests and Cox regression were used to evaluate the prognosis of immune subtypes (IS). Drug sensitivity analysis was also performed for the differences among immune subtypes in immunotherapy and chemotherapy. In addition, dimension reduction analysis based on graph learning was utilized to reveal the internal structure of the immune system and visualize the distribution of patients. Results: Significant differences in prognosis were observed between subtypes (IS1, IS2, and IS3), with the best in IS3 and the worst in IS1. The sensitivity of IS3 to immunotherapy and chemotherapy was better than the other two subtypes. In addition, Immune landscape analysis found the intra-class heterogeneity of immune subtypes and further classified IS3 subtypes (IS3A and IS3B). Immune-related genes were divided into seven functional modules (The turquoise module has the worst prognosis). Five hub genes (RASSF5, CD8A, ICOS, IRF8, and CD247) were screened out as the final characteristic genes related to poor prognosis by low expression. Conclusions: The immune subtypes of TNBC were significantly different in prognosis, gene mutation, immune infiltration, drug sensitivity, and heterogeneity. We validated the independent role of immune subtypes in tumor progression and immunotherapy for TNBC. This study provides a new perspective for personalized immunotherapy and the prognosis evaluation of TNBC patients in the future.

随着现代医学的发展,医学模式正逐渐向社会—心理—生物—医学模式转变,对医务工作者提出更高的要求.如何在培训结束后培养出一名合格的核医学医师,使其不仅具有扎实的理论知识、丰富的实践经验、自我防护的意识,还具有活跃的临床思维?课题组将微信结合CBL教学法应用于核医学住院医师规范化培训中,此种教学模式可以激发培训学员的学习兴趣,丰富教学内容,培养学生独立思考和分析问题的能力,提高学员的临床思维能力.

LASP2 was recently demonstrated to serve as multifaceted roles in several types of cancers. However, its underlying mechanism in the progression of human liver cancer has not been explored. The aims of the current study were to detect LASP2 expression in a liver tissue microarray, and to determine whether LASP2 contributes to malignant phenotypes of HepG2 human hepatoblastoma cells. Our results revealed that LASP2 expression was downregulated in liver cancer tissues relative to normal non-cancerous tissues, and its downregulated expression was closely correlated with malignant process of liver cancer. In vitro, upregulation of LASP2 expression by transfection with LASP2 vector significantly suppressed HepG2 cells viability, colony formation and migration activities. Conversely, the viability, colony formation and migration abilities of HepG2 cells were increased when downregulating LASP2 expression by transfection with small interfering RNA targeting LASP2. Interaction study showed that silencing of LASP2 in HepG2 cells triggered high expression of Cyclin D1, ERK and p-ERK, and low expression of Bax, respectively. In addition, LASP2 silencing-induced malignant phenotypes were further attenuated after HepG2 cells treatment with ERK1/2 blocker PD98059. Collectively, our data suggest a link between LASP2 and MAPK/ERK axis in the development of hepatoblastoma and LASP2 may be a potential marker for assessment of liver cancer prognosis and staging.

Abstract Background: This study will explore the association between Ki-67 expression and clinical pathological characteristics (CPC) of colorectal cancer (CC). Methods: We will search relevant studies from electronic databases (Cochrane Library, PUBMED, EMBASE, Scopus, Cumulative Index to Nursing and Allied Health Literature, China Biology Medicine, and China National Knowledge Infrastructure) from beginning to April 1, 2020 without language and publication time limitations. We will consider all case-controlled studies (CCSs) or randomized controlled studies (RCSs) investigating the association between Ki-67 expression and CPC of CC. We will appraise study quality of CCSs by Newcastle–Ottawa Scale, and RCSs by Cochrane risk of bias tool. Statistical analysis will be carried out by Review Manager 5.3 software. Results: The present study will explore the association between Ki-67 expression and CPC of CC. Conclusion: Its findings may summarize scientific evidence of the association between Ki-67 expression and CPC of CC, and may provide helpful evidence for clinical practice. Systematic review registration: PROSPERO CRD42020173795.

目的:在佳木斯大学临床医学院2015级本科心内科教学中,应用CBL、TBL、PBL融合教学法,通过考核与评价观察并分析教学具体效果.方法:随机选取2015级临床医学本科两个班作为观察班(CBL、TBL、PBL融合教学法)和参照班(传统教学法),观察学习热情,分析教学效果,探讨CBL、TBL、PBL融合教学法应用的可行性及现实意义.结果:观察班学生学习热情、教学效果(理论考核成绩明显提高,P＜0.05)明显高于参照班,有助于培养学生发现问题、分析问题、解决问题以及临床思维能力.结论:CBL、TBL、PBL融合教学法能提高教学质量,提高学生综合素质和创新能力,适合我国当前的医学教学资源状况,具有一定的现实意义,值得推广.

目的:在医疗本科生《生理学》中运用潜科学教学法,通过比较学生成绩和问卷调查来反馈效果.方法:把2018级临床医学专业(1个班)以整群随机抽样法分成两个组(实验组:潜科学教学法;对照组:常规教学法],比较不同教学方式两个组学生的学习成绩差异.结果:对照组成绩明显低于实验组(P＜0.05),且该组的教学评价得分也低于实验组(P＜0.01),统计学上具有明显差异.结论:教学实践中运用潜科学教学法可提升学生的学习热情和成绩,同时培养了临床创新思维,具有可实施性和前瞻性.

Background: This study will explore the association between Ki-67 expression and clinical pathological characteristics (CPC) of colorectal cancer (CC). Methods: We will search relevant studies from electronic databases (Cochrane Library, PUBMED, EMBASE, Scopus, Cumulative Index to Nursing and Allied Health Literature, China Biology Medicine, and China National Knowledge Infrastructure) from beginning to April 1, 2020 without language and publication time limitations. We will consider all case-controlled studies (CCSs) or randomized controlled studies (RCSs) investigating the association between Ki-67 expression and CPC of CC. We will appraise study quality of CCSs by Newcastle-Ottawa Scale, and RCSs by Cochrane risk of bias tool. Statistical analysis will be carried out by Review Manager 5.3 software. Results: The present study will explore the association between Ki-67 expression and CPC of CC. Conclusion: Its findings may summarize scientific evidence of the association between Ki-67 expression and CPC of CC, and may provide helpful evidence for clinical practice. Systematic review registration: PROSPERO CRD42020173795.

目的:观察并分析在佳木斯大学基础医学院2018级临床医学专业五年制生理学教学过程中,采用行为引导型教学联合以问题为导向的教学模式(PBL教学法)后教学质量的改变和效果.方法:将授课学生分为教改组(行为引导型教学+PBL)和常规组(传统教学法),在学期宋授课结束后,进行理论课考试和问卷调查,分析并综合评价两种授课方法的优缺点(学生的临床思维能力、对前沿及基础专业知识的掌握情况、能否促进课前预习等)及可实施性.结果:教改组学生期末总评成绩高(P＜0.05),且学习积极性强、动手实践操作能力大、具备良好的前沿及理论内容掌握度.结论:新形势下在医学教育中合理运用行为引导型教学+PBL教学法,对医学生来说,是一次极好的提升学习热情、学习效率的机会,也是促进自身临床思维能力、以后工作中医患沟通交流能力等的方式之一.具有广阔的推广应用前景和可实施性.

目的:探讨基于案例的教学法教学结合思维导图在儿科住院医师规范化培训中的应用效果.方法:选择2017年1-10月在佳木斯大学附属第一医院儿科参加住院医师规范化培训的30名临床医师作为对照组,选择2017年11月至2018年8月在佳木斯大学附属第一医院参加儿科住院医师规范化培训的28名临床医师作为实验组,分别采用传统教学模式、CBL教学结合思维导图教学模式,比较两组住院规范化培训医师考核成绩、出科时开展规范化培训医师的满意度.结果:经考核,实验组理论知识考核成绩、迷你临床演练评估评分以及总分均显著高于对照组(P＜0.05);经向每位参加住院规范化培训的出科医师进行问卷调查,结果表明,实验组医师出科时满意度优良率达78.57％,显著高于对照组(56.67％)(P＜0.05).结论:CBL教学结合思维导图在儿科住院医师规范化培训中的可行性较强,最大限度地激发了学生的学习兴趣,提高了学生的理论知识掌握度以及临床实践能力水平,临床教学效果十分理想.

Non-coding RNAs such as long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have been found to be indispensable factors in carcinogenesis and cancer development. Numerous studies have explored the regulatory functions of these molecules and identified the synergistic interactions among lncRNAs or miRNAs, while those between lncRNAs and miRNAs remain to be investigated. In this study, we constructed and characterized an lncRNA-miRNA synergistic network following a four-step approach by integrating the regulatory pairs and expression profiles. The synergistic interactions with more shared regulatory mRNAs were found to have higher interactional intensity. Through the analysis of nodes in the network, we found that lncRNAs played roles that are more central and had similar synergistic interactions with their neighbors when compared with miRNAs. In addition, known colon adenocarcinoma (COAD)-related RNAs were found to be enriched in this synergistic network, with higher degrees, betweenness, and closeness. Finally, we proposed a risk score model to predict the clinical outcome for COAD patients based on two prognostic hub lncRNAs, MEG3 and ZEB1-AS1. Moreover, the hierarchical networks of these two lncRNAs could contribute to the understanding of the biological mechanism of tumorigenesis. For each lncRNA-miRNA interaction in the hub-related subnetwork and two hierarchical networks, we performed RNAup method to evaluate their binding energy. Our results identified two important lncRNAs with prognostic roles in colon cancer and dissected their regulatory mechanism involving synergistic interaction with miRNAs.

Background: This study will examine the effects of oxymatrine on the proliferation of human liver cancer Bel-7404 cells (HLCBC). Methods: This study will search electronic bibliographic databases available in PUBMED, EMBASE, Cochrane Library, Scopus, Cumulative Index to Nursing and Allied Health Literature, China Biology Medicine, and China National Knowledge Infrastructure. We attempt to search case-controlled studies (CCSs) or randomized controlled studies (RCSs) pertaining to HLCBC from their inception to the February 29, 2020 without limitations of language and publication time. We will include any CCSs or RCSs on exploring oxymatrine on the proliferation of HLCBC. We will assess the methodological quality of CCSs by Newcastle-Ottawa Scale, and RCSs by Cochrane risk of bias tool. Review Manager 5.3 software will be utilized for statistical analysis. Results: The current study will summarize most recent eligible studies to investigate the effects of oxymatrine on the proliferation of HLCBC. Conclusion: Its results may provide reliable scientific evidence on effects of oxymatrine on the proliferation of HLCBC.

Abstract Background: This study will examine the effects of artemisinin on proliferation and apoptosis of human liver cancer HepG2 cells (HLCHG-2C). Methods: This study will systematically retrieve potential literatures in MEDLINE, Scopus, Web of Science, Cochrane Library, EMBASE, WANGFANG, and China National Knowledge Infrastructure from their initiation to the February 29, 2020. There are not limitations related to the language and publication time. All case-controlled studies (CCSs) or randomized controlled studies (RCSs) will be included in this study which investigated the effects of artemisinin on proliferation and apoptosis of HLCHG-2C. Two independent investigators will examine searched records, collect data from included studies, and will identify their methodological quality. Any divergences will be disentangled by discussion with another investigator. RevMan 5.3 software will be placed to pool the data and to carry out data analysis. Results: This study will summarize all eligible studies to test the effects of artemisinin on proliferation and apoptosis of HLCHG-2C. Conclusion: The results of this study will exert evidence to examine the effects of artemisinin on proliferation and apoptosis of HLCHG-2C, and it may benefit further research, patients, and healthcare providers. Systematic review registration: INPLASY202040075.

为了探讨微信结合CBL教学法在核医学住院医师规范化培训中的应用效果.本课题组将2019年9月来核医学科规培的48名住院医师作为研究对象,随机分成实验组和对照组.实验组采用微信结合CBL教学法进行教学,对照组采用传统教学.每学期规培结束后,进行理论和实践技能考核,评价两种教学法的优劣.结果显示,实验组理论和实践技能考核成绩均明显优于对照组,差异有统计学意义(P<0.05).因此,将基于微信平台的CBL教学法应用于核医学住院医师规范化培训中,明显提高了住院医师的理论和实践能力,为住院医师规范化培训提供了新的思路.

Abstract Background: This study will examine the effects of oxymatrine on the proliferation of human liver cancer Bel-7404 cells (HLCBC). Methods: This study will search electronic bibliographic databases available in PUBMED, EMBASE, Cochrane Library, Scopus, Cumulative Index to Nursing and Allied Health Literature, China Biology Medicine, and China National Knowledge Infrastructure. We attempt to search case-controlled studies (CCSs) or randomized controlled studies (RCSs) pertaining to HLCBC from their inception to the February 29, 2020 without limitations of language and publication time. We will include any CCSs or RCSs on exploring oxymatrine on the proliferation of HLCBC. We will assess the methodological quality of CCSs by Newcastle-Ottawa Scale, and RCSs by Cochrane risk of bias tool. Review Manager 5.3 software will be utilized for statistical analysis. Results: The current study will summarize most recent eligible studies to investigate the effects of oxymatrine on the proliferation of HLCBC. Conclusion: Its results may provide reliable scientific evidence on effects of oxymatrine on the proliferation of HLCBC. Systematic review registration: INPLASY202040026.

CXCL3 belongs to the CXC-type chemokine family and is known to play a multifaceted role in various human malignancies. While its clinical significance and mechanisms of action in uterine cervical cancer (UCC) remain unclear. This investigation demonstrated that the UCC cell line HeLa expressed CXCL3, and strong expression of CXCL3 was detected in UCC tissues relative to nontumor tissues. In addition, CXCL3 expression was strongly correlated with CXCL5 expression in UCC tissues. In vitro, HeLa cells overexpressing CXCL3, HeLa cells treated with exogenous CXCL3 or treated with conditioned medium from WPMY cells overexpressing CXCL3, exhibited enhanced proliferation and migration activities. In agreement with these findings, CXCL3 overexpression was also associated with the generation of HeLa cell tumor xenografts in athymic nude mice. Subsequent mechanistic studies demonstrated that CXCL3 overexpressing influenced the expression of extracellular signal-regulated kinase (ERK) signaling pathway associated genes, including ERK1/2, Bcl-2, and Bax, whereas the CXCL3-induced proliferation and migration effects were attenuated by exogenous administration of the ERK1/2 blocker PD98059. The data of the current investigation support that CXCL3 appears to hold promise as a potential tumor marker and interference target for UCC.

Multiple previous studies have demonstrated that the dysregulation of microRNAs (miRNAs) is implicated in the occurrence and development of pancreatic cancer. Therefore, a further characterisation of deregulated miRNAs in pancreatic cancer may provide novel insight into the oncogenesis and progression of pancreatic cancer, which may facilitate the identification of effective therapeutic targets for treating patients with this disease. In the present study, reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that the expression level of miRNA‑584‑5p (miR‑584) was significantly decreased in pancreatic cancer tissues and cell lines. It was demonstrated that restoration of miR‑584 expression significantly suppressed the proliferative and invasive ability of pancreatic cancer cells. Bioinformatics analysis predicted that cyclin D1 (CCND1) was a putative target of miR‑584. Subsequent experiments demonstrated that CCND1 was a direct target gene of miR‑584 in pancreatic cancer cells. Furthermore, the inhibition of CCND1 mimicked the suppressive effect of miR‑584 overexpression in pancreatic cancer cells. The restoration of CCND1 expression significantly abolished the inhibitory effects of miR‑584 overexpression on pancreatic cancer cells. Collectively, the present results demonstrated that miR‑584 inhibited the development of pancreatic cancer by directly targeting CCND1, suggesting that this miRNA may represent a potential therapeutic target for this fatal disease.

This study retrospectively investigated the effect of neuromuscular electrical stimulation (NMES) for fatigue management in patients with advanced laryngeal cancer (ALC) receiving chemoradiotherapy.A total of 60 eligible patients with ALC receiving chemoradiotherapy were included. These patients were assigned equally to a treatment group and a control group. Patients in the treatment group received NMES therapy and were treated for a total of 8 weeks, while the patients in the control group did not receive NMES therapy. The primary outcome was fatigue, measured by the multidimensional fatigue inventory (MFI). The secondary outcomes included anxiety and depression, measured by the Hospital Anxiety and Depression Scale (HADS), and sleep quality, measured by the Pittsburgh Sleep Quality Index (PSQI). All outcomes were evaluated before and after 8-week NMES treatmentAfter 8-week NMES treatment, the patients in the treatment group did not exert better effect than patients in the control group in fatigue relief, measured by the MFI score, anxiety and depression decrease, assessed by HADS, and sleep quality improvement, evaluated by PSQI.The results of this study demonstrate that NMES may not benefit for fatigue relief in patients with ALC receiving chemoradiotherapy. Future studies should still focus on this topic and warrant these results.