目的 探讨田七总色素的体外抗氧化活性.方法 以蒸馏水为溶剂,从田七中提取总色素;采用比色法分别测定田七总色素清除DPPH·、O-2·和·OH三种自由基的作用,以及对Fe3+的还原能力.结果 田七总色素对三种自由基都有一定的清除作用,清除DPPH·、O-2·自由基的EC50分别是0.17 mg/ml、0.24 mg/ml;当色素浓度为0.15 mg/ml时,其对DPPH· 清除率为45.03% 、对O-2·为23.48% 、·OH 清除率为3.71%,其对三种自由基的清除能力排序为DPPH· > O-2· >·OH;对Fe3+ 也具有较强的还原能力.结论 氧自由基清除率与田七总色素浓度存在明显的量效关系,清除作用随着总色素浓度的增加而增强.还原Fe3+能力也随总色素的浓度增加而增强.

Study on the antioxidant of the flavanone in the Longan leaves.The flavonoids were extracted with w(Ethanol) =95 % as the solvent from Longan leaves by water bath backflow.It will determine the flagstone's scavenging process acting on DPPH.,.OH and O2-.with the Spectrophotometry,and the reduction of Fe3 + ability.Total flavanone from Longan leaves have significant scavenging effect to three kinds of free radicals,the result of scavenging process is that DPPH.>.OH > O2-.;moreover it also have strong ability of reduction to Fe3 +.Clearance and concentration obvious concentration-response relationship.When the content of the total flavanone of Longan leaves was ρ(Flavanone)=0.07 mg/L,the clearance rate of DPPH.,.OH and O2-.was 58.8 %,52.5 % and 16.5 %.Evident reduction energy of the flavanone was increasing by the concentration.

Objective To study the antioxidant effect of the pigments in the jasmine leaves. Methods Using the distilled water as the solvent to extract pigments from the jasmine leaves; It will determine the scavenging process of pigments in the jasmine leaves acting on DPPH·,O2-· and ·OH with the spectrophotometry and the reduction of Fe3 +ability. Results Total pigments from jasmine leaves have significant scavenging effect to three kinds of free radicals,the result of scavenging process is that DPPH· >O2-· > ·OH; moreover,they also have strong ability of reduction to Fe3 +. Conclusion Clearance and concentration obvious concentration-response relationship. When the content of the total pigments of the jasmine leaves was ρ( Pigments) = 3. 5 mg /ml,the clearance rate of DPPH·,O2-oand ·OH was 49. 3%,55. 1% and 20. 9%. The reduction ability of Fe3 +to the pigments was enhanced when there concentration was increasing.

目的:为生产各种食用品、化妆品、医药药品等色素新产品提供重要原料,研究香附色素的提取条件和稳定性.方法:以香附为材料,以蒸馏水作提取剂,用紫外分析法研究色素稳定性.结果:以蒸馏水为提取剂,料液比为1:100,在室温下浸泡24小时.结论:该色素在215nm处有最大吸收峰,其水溶性较好,强酸性物质对该色素的稳定性影响较大,但热稳定性、耐光性良好.葡萄糖、淀粉对色素的稳定性影响较大,而醋酸、防腐剂、蔗糖无明显影响.氧化剂、还原剂中除过氧化氢对色素的影响明显外,其他均无明显影响.除Fe3+外,金属离子对其影响较小.

To study the antioxidant effect of the pigments in the Hawthom.Using the distilled water as the solvent to extract pigments from the Hawthom;It will determine the scavenging process of pigments in the Hawthom acting on DPPH.、O2-.and.OH with the spectrophotometry;and the reduction of Fe3+ ability.Total pigments from Hawthom have significant scavenging effect to three kinds of free radicals,the result of scavenging process is that DPPH.>.OH>O2-.;moreover,they also have strong ability of reduction to Fe3+.Clearance and concentration obvious concentration-response relationship.When the content of the total pigments of the Hawthom was P(pigments)=1.7 mg/mL,the clearance rate of DPPH.、O2-.and.OH was 56.1%、11.5% and 42%.The reduction ability to Fe3+ of the pigments was enhanced when their concentration was increasing.

Objective: To study the antioxidant effect of the pigments in Prunella vugaris.Methods: Using distilled water as the solvent to extract pigments from Prunella vugaris.It will determine the scavenging process of pigments in Prunella vugaris acting on DPPH.、O2-.and.OH with the spectrophotometry;and the reduction of Fe3 + ability.Results: Total pigments from Prunella vugaris have significant scavenging effect to three kinds of free radicals,the result of scavenging process is that DPPH.>.OH > O2-.;moreover,they also have strong ability of reduction to Fe3 +.Conclusion: Clearance and concentration obvious concentration-response relationship.When the content of the total pigments of Prunella vugaris was ρ(Pigments) = 0.13 mg / mL,the clearance rate of DPPH.、O2-.and.OH was 28.3%、4.1% and 17.9%.The reduction ability to Fe3 + of the pigments was enhanced when their concentration was increasing.