Serological tests based on an agglutination reaction are commonly applied in clinical laboratories for the diagnosis of various infectious diseases. There is a wide selection of microsphere-based diagnostic agglutination tests (yes/no results) and assays (quantitative results) worldwide. However, due to rapidity and no need for special equipment passive hemagglutination tests are still the most frequently used routine tests in Russia. These hemagglutination tests are run in 96-well microplates with V-shaped bottoms. The substitution of red blood cells for polymer microspheres in such semi-quantitative tests is difficult due to a number of reasons. When choosing the appropriate microspheres for rapid passive latex agglutination tests, it is important to consider such variables as their size (and size distribution), density and colloidal stability. In our study we showed the development of rapid (1-3 hours) passive latex agglutination tests based on the knowledge of sedimentation stability of polymer suspensions. We studied sedimentation rates of sheep and chicken erythrocytes used in passive hemagglutination tests and identified the required intervals of sedimentation rate for polymer microspheres. Based on these dates a theoretical model of polymer microsphere motion within a V-shaped well was proposed, which revealed that the formation of a lattice in the passive latex agglutination reaction takes place not in the volume of the well, but on the surface of the well bottom.

Serological tests based on an agglutination reaction are commonly applied in clinical laboratories for the diagnosis of various infectious diseases. There is a wide selection of microsphere-based diagnostic agglutination tests (yes/no results) and assays (quantitative results) worldwide. However, due to rapidity and no need for special equipment passive hemagglutination tests are still the most frequently used routine tests in Russia. These hemagglutination tests are run in 96-well microplates with V-shaped bottoms. The substitution of red blood cells for polymer microspheres in such semi-quantitative tests is difficult due to a number of reasons. When choosing the appropriate microspheres for rapid passive latex agglutination tests, it is important to consider such variables as their size (and size distribution), density and colloidal stability. In our study we showed the development of rapid (1-3 hours) passive latex agglutination tests based on the knowledge of sedimentation stability of polymer suspensions. We studied sedimentation rates of sheep and chicken erythrocytes used in passive hemagglutination tests and identified the required intervals of sedimentation rate for polymer microspheres. Based on these dates a theoretical model of polymer microsphere motion within a V-shaped well was proposed, which revealed that the formation of a lattice in the passive latex agglutination reaction takes place not in the volume of the well, but on the surface of the well bottom.

The influence of ammonium sulfate concentration in aqueous sybfase on the parameters of 2D films was investigated. It was observe that, adding of electrolyte is causing the growth of parameters of isotherm due to gidrophobisacia of microsphere because of changes in double electrical layer.

The influence of ammonium sulfate concentration in aqueous sybfase on the parameters of 2D films was investigated. It was observe that, adding of electrolyte is causing the growth of parameters of isotherm due to gidrophobisacia of microsphere because of changes in double electrical layer.

The morphology of 2D films of fullerene C60 on interfaces has been studied by Brewster angle microscopy and atomic force microscopy. Fullerene C60 tends to aggregate, forming supramolecular structures with a surface area per C60 molecule from 21.6 to 2900 Å2. As the area per C60 molecule decreases, monomolecular clusters gradually transform into multiplayer structures. The introduction of an electrolyte into the system prevents the formation of fullerene globules and favors the formation of more homogeneous films.

The effect of ammonium sulfate on the parameters of surface pressure isotherms (surface pressure and area per fullerene molecule) of 2D films of fullerene C60 on the water/air interface has been studied by the Langmuir method. The possibility of regulation of the structure of 2D fullerene films has been shown. The maximal A 0 value is achieved at an ammonium sulfate concentration of 1 × 10−4 mol/L. Brewster angle microscopy shows that the presence of the electrolyte ensures the formation of a monomolecular 2D film with a lower content of defects.