<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">本试验旨在探讨不同生长环境对同源湖羊生长性能、瘤胃内容物微生物组成及生物信息的影响。选择遗传背景相似、同胎次的1岁左右的湖羊90只（公∶母=1∶8）,其中45只运输至青海省海晏某肉羊繁育基地饲养繁育,另外45只留在山东高密某牧业有限公司饲养繁育。当年配种,次年产羔。2个地方所产的羔羊均75日龄断奶,标记,作为备选试验对象。试验分为2组,山东组（sdlw组）和青海组（qhlw组）。每组按照体重相近的原则挑选90日龄健康湖羊40只,随机均分至4个饲养栏中（公母混养）,开始常规饲养试验。预试期10 d,正试期150 d。试验结束后每组随机选取4只公羊剖杀并采集瘤胃液,应用16s RNA技术分析细菌菌群差异及Tax4Fun功能预测。结果表明:1)在饲粮营养物质基础相近的情况下,青海组和山东组平均日增重差异显著（P&lt;0.05）,料重比差异显著（P&lt;0.05）;饲养在山东和青海的湖羊瘤胃内容物菌群丰富度无显著差异（P&gt;0.05）。2)基于Unweighted Unifrac距离主成分分析（PCoA）发现,山东组和青海组组内物种相似性较大,组间物种多样性方面存在较大差异。3)物种分析发现,2组在门水平上,优势菌门有拟杆菌门、厚壁菌门、绿弯菌门,在属水平上,优势菌属为未鉴定普雷沃氏菌科、未鉴定瘤胃菌科、拟杆菌属;优势菌种为普雷沃菌、瘤胃杆菌、栖瘤胃普雷沃菌。在门水平上,山东组绿弯菌门相对丰度显著高于青海组（P&lt;0.05）,在属水平上,青海组未鉴定普雷沃氏菌科相对丰度显著高于山东组（P&lt;0.05）;而未鉴定瘤胃菌科相对丰度显著低于山东组（P&lt;0.05）。4)通过LEfSe分析发现,青海组中起重要作用的为普雷沃菌属、普雷沃氏菌科,而山东组中起重要作用的为瘤胃菌科、g_Flexilinea、厌氧绳菌纲、c_Anaerdlineales、绿弯菌纲、厌氧绳菌科。5)通过对生物代谢通路一级功能层分析发现,山东组在环境信息处理、细胞代谢和新陈代谢等代谢通路的基因表达丰度较青海组高;对二级功能层分析发现,山东组在转录、氨基酸代谢、碳水化合物代谢、膜运输、脂质代谢、细胞群落原核生物、新陈代谢、细胞过程和信号传导等方面基因表达丰度明显高于青海组。综上所述,在饲粮营养物质基础相近的情况下,青海组和山东组湖羊平均日增重差异显著,料重比差异显著;不同生长环境可以显著改变同源湖羊瘤胃内容物微生物组成与多样性;饲喂在山东的湖羊在转录、营养物质代谢、氨基酸代谢、碳水化合物代谢、脂质代谢、新陈代谢、膜运输、细胞群落原核生物、细胞过程和信号传导等方面基因丰度的表达优于饲喂在青海的湖羊。</span>

<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">本试验旨在探讨复合微生态制剂联合低聚果糖饲喂哺乳期犊牛对其生长性能、腹泻率、肠道微生态区系的影响,选取21头3日龄健康荷斯坦母犊,将其随机均分成3组:ZK组每日每头添加植物乳酸杆菌（1×10⁹CFU）和枯草芽孢杆菌(2×10¹⁰CFU)各5 g;ZKT组每日每头添加植物乳酸杆菌（1×10⁹CFU）、枯草芽孢杆菌(2×10¹⁰CFU)和低聚果糖各5 g（上述2组在常规饲喂后1 h将相应的制剂溶解在温水中用奶瓶灌入）;C组为空白对照组,常规饲喂。预试期7 d,正试期50 d。结果表明:1)ZK组和ZKT组平均日增重和平均日采食量与C组相比差异不显著（P&gt;0.05）,但这2组腹泻率显著低于C组（P&lt;0.05）。2)微生物群落Alpha多样性分析表明,添加微生态制剂对ZK组犊牛肠道微生物区系丰富度和多样性影响不显著（P&gt;0.05）,但对ZKT组肠道微生物丰度影响显著（P&lt;0.05）。对肠道微生态区系分析发现,在门水平上,优势菌门主要包括拟杆菌门、厚壁菌门、无壁菌门,排名前10位的菌门相对丰度各组之间差异不显著（P&gt;0.05）;在属水平上,优势菌属为拟杆菌属、未鉴定普雷沃氏菌科、别样棒菌属,排名前10位的菌属在ZKT组拟杆菌属相对丰度显著高于C组和ZK组（P&lt;0.05）。3)通过t-检验分析差异物种发现,ZKT组梭状芽孢杆菌相对丰度显著低于C组（P&lt;0.05）。综上所述,本研究2种制剂对犊牛生长性能影响不大,ZK组制剂的添加对其肠道微生态区系影响不显著,但ZKT组制剂可显著改变犊牛肠道微生物区系,使其拟杆菌属相对丰度显著增加,尤其可显著减少肠道梭状芽孢杆菌相对丰度,进而显著减少了腹泻的发生。</span>

为了探索有益菌降解霉菌毒素、抵抗病原菌、分泌消化酶的效能.采用微生物培养技术、酶联免疫(ELISA)检测技术、药敏试验及常规酶类检测方法,对初步筛选的8株有益菌(其中2株乳酸菌,编号R-2、R-3;4株芽胞菌,编号Y-1、Y-2、Y-3、Y-4和2株酵母菌,编号J-4、J-6)进行了系统的试验.结果表明,8株不同类型的有益菌对黄曲霉毒素均有一定的降解作用,其中降解率较高的菌株R-3为54.5％、Y-2为58.72％、J-4和J-6分别为90.57％和73.21％;对呕吐毒素降解率最高的是Y-2(51.57％);对玉米赤霉烯酮降解率效果最好的是Y-1和Y-2,分别为56.57％和70.70％.在抑菌方面,乳酸菌R-2、R-3菌株和芽胞菌Y-1、Y-2菌株对致病性大肠杆菌、沙门氏菌和金黄色葡萄球菌均具有很强的抑制杀灭作用;芽胞菌Y-1、Y-2菌株还具有分泌蛋白酶、淀粉酶及纤维素酶的能力.结论:8株有益菌不但能够降解霉菌毒素,而且还具有抗菌和产酶能力.

<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">本试验旨在探讨复合微生态制剂对哺乳期犊牛生长性能及瘤胃微生态区系的影响。选取3日龄健康荷斯坦母犊牛21头,随机分成3组,每组7头,单栏饲养。空白对照组（C组）犊牛饲喂基础饲粮,植物乳酸杆菌+枯草芽孢杆菌组（ZK组）每日每头犊牛饲喂基础饲粮+5 g植物乳杆菌+5 g枯草芽孢杆菌,植物乳杆菌+枯草芽孢杆菌+低聚果糖组（ZKT组）每日每头犊牛饲喂基础饲粮+5 g植物乳杆菌+5 g枯草芽孢杆菌+5 g低聚果糖。ZK组和ZKT组犊牛均在饲喂基础饲粮1 h后将相应的复合微生态制剂溶解在温水中,用奶瓶灌入。预试期7 d,正试期50 d。结果表明:各组之间平均日增重和平均日采食量无显著差异（P&gt;0.05）。微生物群落Alpha多样性分析表明,各组之间可见物种数量、Chao1指数、ACE指数、Shannon指数及Simpson指数无显著差异（P&gt;0.05）;Beta多样性分析表明,ZKT组基于非加权距离主成分分析聚类较好,C组与ZK组相对分散,说明组间群落结构差异较大。以非加权距离矩阵做UPGMA聚类分析,聚类树不能将C组有效区分,ZK组聚类较好,ZKT组聚类最好,说明复合微生态制剂的添加可影响犊牛微生态区系。瘤胃内容物细菌菌落组成分析发现,犊牛瘤胃内容物细菌优势菌门为拟杆菌门、厚壁菌门、放线菌门等,优势菌属为欧陆森氏菌属、未鉴定普雷沃氏菌科、醋香肠菌属等。Tax4Fun功能预测显示,ZK组的基因表达丰度在细胞过程、环境信息处理和未鉴定功能3个一级功能层较C组增高,ZK组的基因表达丰度在遗传信息处理、感染性疾病、氨基酸代谢、其他氨基酸代谢、碳水化合物代谢5个二级功能层比ZKT组、C组升高。综上所述,复合微生态制剂对哺乳期犊牛生长性能和瘤胃菌群丰富度及多样性无显著影响;而ZKT组哺乳期犊牛断奶前的瘤胃微生态区系明显改变,ZK组在功能预测上要好于ZKT组。</span>

为建立传染性法氏囊病病毒(IBDV)对雏鸡的最佳感染模型,采用点眼滴鼻、肌肉注射、擦肛和泄殖腔内灌注等不同方法,对SPF鸡和普通鸡进行了攻毒致病试验.结果表明,以泄殖腔内灌注方法攻毒,可使雏鸡发生传染性法氏囊病(IBD)速度快,病死率高,临床症状和病理变化典型.该试验结果为制备鸡传染性法氏囊病感染模型,提供了精确、简单的技术支持.

本文旨在研究新型(降解霉菌毒素)微生态制剂对蛋鸡生产性能和蛋品质的影响.选取420d健康海兰褐蛋鸡1296只,随机分为4个处理,每个处理6个重复,每个重复54只.试验处理如下:对照组,饲喂基础日粮;试验I、II、III组分别在对照组的基础上添加0.05％、0.10％、0.20％的微生态制剂.试验期为28d.饲养期间每周统计周采食量.每天以重复为单位捡蛋一次,记录产蛋数、蛋重、破蛋数,计算平均蛋重、产蛋率、破蛋率和料蛋比.于试验第14、28天,每个重复取20枚鸡蛋测定蛋品质.试验结果表明,与对照组相比,3个试验组蛋鸡的平均蛋重均显著提高(P<0.05),破蛋率和料蛋比均显著降低(P<0.05);与试验I组相比,试验II、III组蛋鸡的破蛋率和料蛋比均显著降低(P<0.05);与对照组相比,第14、28天试验组的蛋壳厚度均显著提高(P<0.05),第28天试验II、III组鸡蛋的蛋黄比重均显著提高(P<0.05);与试验I组相比,试验II、III组第28天鸡蛋的蛋黄比重显著提高(P<0.05).综上所述,微生态制剂能提高产蛋后期蛋鸡的生产性能和蛋品质,提高蛋鸡生产的经济效益,综合比较以0.10％的量添加时的效果最佳.

本文对禽C3d的分子结构,对抗原免疫原性的影响做了初步研究.结果表明,理论上本动物C3d的免疫增强效果更好.

To improve the efficacy of porcine reproductive and respiratory syndrome virus (PRRSV) subunit vaccine, the immunological enhancement effects of four natural adjuvants were compared, including Astragalus extract, Astragalus/Bacillus production, propolis, and Freund's adjuvant. The results showed that titers of IgG and neutralizing antibody against highly pathogenic PRRSV (HP-PRRSV) glycoprotein 5 (GP5) from the groups of Astragalus/Bacillus adjuvant and Freund's adjuvant co-administered with a recombinant HP-PRRSV GP5 subunit were the highest among all the experimental groups. In cellular immunity, as shown in T lymphocyte proliferation level, Astragalus/Bacillus adjuvant and Freund's adjuvant exhibited the most potent immunological enhancement effects. Exceptionally, pigs inoculated with Freund's adjuvant developed severely delayed humoral immune responses within 28 days post inoculation. The adjuvants of Astragalus extract and propolis also exhibited immunological enhancement effects on humoral immune and cell-mediated immune responses, but which were obviously lower than Astragalus/Bacillus adjuvant and Freund's adjuvant. Following challenge with HP-PRRSV, pigs inoculated with Astragalus/Bacillus adjuvant and Freund's adjuvant co-administered with GP5 subunit showed lower viremia, slighter clinical signs, and less pathological lung lesions compared with the groups inoculated using Astragalus extract and propolis. In conclusion, from immune responses and immunological protection against HP-PRRSV, the Astragalus/Bacillus adjuvant demonstrated the most potent immunological enhancement effect on HP-PRRSV GP5 subunit. Astragalus/Bacillus adjuvant showed promise as a candidate immune adjuvant, to more efficiently prevent and control PRRS.

什么是微生态制剂？一般地说，从健康动物体内分离的有益菌，按照科学的工艺加工制成的活的制剂，称为微生态制剂。微生态制剂分三类，一类叫益生菌，也叫益生素，也就是有益微生物。益生元是促进有益微生物生长的物质，就像低聚糖，低聚糖本来是夹在人的食品当中作为一种保健品，在我们消化道的上端不能分解利用，也就是说，我们机体不能分解低聚糖的这种酶，它到了下消化道，下消化道有能够分泌、分解低聚糖的酶，使有益微生物大量生长，像双歧杆菌和乳酸菌大量生长，能促进有益微生物的大量生长，这样就促进了机体的健康。促进有益微生物生长的物质，叫益生元。当然有很多物质，比如胡萝卜素以及牛奶当中的成分，像吃的水果当中的香蕉、地瓜，这些都有正常微生物的促生长物质，我们也把它称为益生元。合生元就是有益菌和这些促生长物质配合在一起制成的制剂。不同国家对允许使用的有益微生物的菌种有规定，我们国家也是如此。

In order to investigate histo-pathological changes of chickens induced by glandular stomach(GS)type avian infectious bronchitis virus and the ultra-structure of the virus,clinical dissection,histo-pathology and electron microscopic examination were conducted over the 3 Shandong isolates(QX,DY and FC) of stomach type avian infectious bronchitis virus and the ultrastructure of the virus.The results showed that the isolates induced significant glandular stomach tissue inflammation manifested mainly in inflammatory cell infiltration in proventriculus mucosa,glandular epithelial hyperplasia,mucus and abundant necrotic,exfoliated and disintegrated glandular cells in gland lumen,monocyte infiltration and abundant vacuolation in epithelial cells.Viral particles with 80~120 nm were observed in the pathological tissues.Virus isolated via chicken embryo was too 80~150 nm in size with spherical or pear-shaped form.The virus was enveloped with 12~25 typical coronoid projections(the clustered virus has few coronoid projections),thus named as coronavirus.The study provided scientific reference for further studies and effective prevention and control of glandular stomach type avian infectious bronchitis.

In order to understand the pathogenicity of proventriculus type infectious bronchitis virus(IBV),3IBV strains(QXIBV,DYIBV and FCIBV)were used to challenge the unimmunized chickens and SPF chickens via nose,eye and mouth dripping in regression tests.In result,the post-mortem changes of chickens died from virus challenge in 10-day-old unimmunized were similar to those in 20-day-old unimmunized chickens.Both showed glandular stomach swelling,proventriculus mucosa hemorrhage and ulcer,kidney swelling and uratosis.The mortality of 20-day-old challenged chickens was somewhat higher than that of the 10-day-old challenged chickens.All the challenged SPF chickens showed clinical signs of depression,puffed feather and diarrhea,pathological changes of glandular stomach swelling,proventriculus mucosa hemorrhage and ulcer,and kidney swelling and uratosis which were similar to those in the naturally-infected birds.The result showed that clinical signs and pathological changes similar to those in the naturallyinfected birds could be reproduced using the Shandong IBV isolates.

In order to characterize the nucleotides of spike protein S genes of three Shandong isolates(Qingdao Strain QX,Dongying Strain DY and Feicheng Strain FC)of proventriculus type avian infectious bronchitis virus(IBV),and to compare the homology of their S gene nucleotides with respiratory type IBV M41 and nephric type IBV,RT-PCR was used to clone and sequence the S genes of the IBV isolates(QX,DY and FC)and the reference strain M41.The S genes of QX and DY strains were sequenced by Clustal V method,Jotun Hein method and Clustal W method respectively.The results showed that the highest homology of the S gene nucleotides of Strains QX and DY with the reference strain M41 was up to 79.3%and79.2%respectively with the least variation of 24.5%and 22.3%;the highest homology of the S gene nucleotides of Strains QX and DY with nephric type IBV Strain HD was up to 78.4%and 78.9%,both with the least variation of 22.7%.The highest homology between Strain HD of nephric type IBV and M41 was up to 80.8%.Analysis of the S gene evolution relationship indicated that the proventrivulus type IBV Shandong isolates(QX and DY)were variant strains with different tissue tropism and with significant difference from the respiratory type and nephric type IBV,and possessed an important evolution stage.

为探讨微生物发酵对绞股蓝(GP)有效成分、肉鸡生产性能和免疫功能的影响,将150只AA肉鸡随机分成6组,分别饲喂不同GP发酵液和微生态制剂(由YB1、YB18和JM6按1∶1∶1比例配制而成),按照1 kg兑水600 kg浓度饮水给药,具体分组:1组饲喂微生态制剂(11～28日龄);2组饲喂微生态制剂(11～15日龄,21 ～ 28日龄)和YB1+YB18+JM6+GP(酵GP)(6～10日龄,16～20日龄);3组饲喂YB1 +YB18 +GP(11 ～28日龄);4组饲喂JM6+GP(11 ～28日龄);5组饲喂未发酵GP;6组为空白对照组.试验采用益生菌对GP进行液体发酵,以GP发酵液饮水饲喂肉鸡,采用硫酸香草醛法测定GP发酵前后有效成分变化,并对肉鸡体重、成活率和血清γ干扰素(IFN-γ)水平进行测定.结果表明:在不同时间点,GP发酵液中GP总皂苷含量均不同程度升高,在20日龄时,2,4组肉鸡体重显著高于对照组(P＜0.05);在28日龄时,2,3,5组肉鸡体重均显著高于对照组(P＜0.05);在36日龄时,1,2,3组肉鸡体重显著高于对照组(P＜0.05),2,3组肉鸡体重显著高于5组(P＜0.05);在40日龄时,2,3组肉鸡体重显著高于对照组(P＜0.05).GP发酵液组肉鸡成活率和血清IFN-γ水平均显著高于对照组(P＜0.05).说明微生物发酵能提升GP有效成分含量,是提升GP增强肉鸡生产性能和免疫功能效果的一种新方法.

(Purpose) In order to that ascertain the effect on immune function and growth performance of pigs was studied fermentation beds of different padding.(Methods) 40 pigs were randomly divided into 4 groups,each group included 10 pigs.The first group is the feeding group with the fermentation beds of using crushed corn stalks mainly(corn stalks group);Group 2 is the feeding group with fermentation beds of using peanut-shell mainly(peanut-shell group);Group 3 is the feeding group with fermentation beds of using sawdust mainly(sawdust group);The forth group is the feeding group with conventional concrete floor(control group).Each group was injected with swine fever vaccine,PRRSV vaccine and swine pseudo-rabies vaccine,and was fed with according to conventional breeding methods.The experiment lasted 60 days.Rate of weight gain of each pig,serum antibody positive rate,γ-IFN and IL-4 levels,hemoglobin in the blood and the total number of lymphocytes and erythrocytes were determined at the end of experiment.(Results) The results indicate that when fed to the 60 days,the rate of weight gain of pigs in the group 1,group2 and group 3 were higher than the control group.The higher rates were separately 4.66%,7.16% and 3.56%.And the difference was significant(P 0.05).The antibody positive rate of pigs in group 1,group 2 and group 3 were higher than control group.The difference of group 1 was not significant,and the difference of group 2 and group 3 was significantly different(P 0.0001).The blood hemoglobin content,red blood cell volume and the total number of lymphocytes in group 1 were lower than the control group,and these of group 2 and 3 were both higher than the control group.And there was no significant difference.The levels of γ-IFN and IL-4 in pig serum of group 1,group 2 and group 3 were higher than the control group.But there was no significant difference.(Conclusion) Compared with the feeding group with the conventional concrete floor,the feeding groups with fermentation beds of using different padding all can improve pig growth performance and immune effects.And the fermentation beds of using corn stalks and peanut shells can significantly improve pigs' rate of weight gain growth and feed utilization.A theoretical basis and technical support of extension and application with the technology of rearing pigs with fermentation beds was provided by the results of the study.

Adhesive ability was tested in seven Lactobacillus solated from the chicken digestive tract after cultivation with CaCo-2 cells (intestinal epithelial cells), MDCK (dog kidney) cells and CEF (chicken embryo fiber) cells. We noted the following important observations regarding the adhesive ability between different Lactobacillus strains and three cell types: the adhesive interaction between the SDnB7, SDnE1 and SDnA3 lactobacillus strains and CaCo-2cells was greater compared to controls, the adhesive effect between SDnB1 and CEF cells was also greater than controls and Lactobacillus showed only minimal adherence to MDCK cells. Incubation time also affected Lactobacillus adherence to CaCo-2cells: adhesive ability was optimal at 37°C when incubated for 2 days and this was confirmed by a local increase in the concentration of Lactobacillus around CaCo-2 cells when incubated for 24 h as opposed to 3 h. Adherence ability in lactobacillus was also tested at various concentrations (108, 107, 106, 105 and 104 ). The number of Lactobacillus that adhered around the cells was significantly increased in the treatment with 108 bacterial cells. Transmission electron microscopy revealed that the cellularity of the junction between CaCo-2cells and Lactobacillus was not compromised. Transmission electron microscopy also revealed that the thalli fabric structure remained intact.

Use of antiserum of hare resist chicken digestive tract epithelial cells Lactobacillus adhesion match protein,by discontinuation alive-PAGE electrophoresis and indirected ELISA technology,detected Lactobacillus adhesion match protein content in crop and intestine of 1-40-day-old healthy and coccidiosis chicken.The results suggested that 2-day-old chicken crops have Lactobacillus adhesion receptor protein,D450 nm is 0.181,1-day-old chicken intestines have Lactobacillus adhesion receptor protein,D450 nm is 0.168.Its stabilization is at 5 day,D450 nm is 0.200 and 0.123 respectively.Healthy chicken crop and intestines have more Lactobacillus adhesion match protein than coccidiosis chicken.D450 nm is 0.143,0.132 in crop;0.148,0.134 in intestines respectively.

Lactobacillus adheres to intestinal epithelial cells and yeast fungus cells with the aid of adhesion factors expressed on its cell surface. To identify adhesion factors nature on the surface of Lactobacillus, an adhesion experiment was carried out by pre-treating the Lactobacillus supernatant with different concentrations of bovine serum albumin, trypsin and 100°C for 10min. Additionally, intestinal epithelial cells were treated with sodium iodate, trypsin and sugar inhibition tests to characterize the receptors in Lactobacillus that interact with intestinal epithelial cells. It was demonstrated that Lactobacillus adhesion ability was decreased (P Lactobacillus cells was identified as a D-mannose glycoprotein. This observation was confirmed after treating intestinal epithelial cells with sodium iodate and trypsin, and sugar inhibition tests. Wild type Lactobacillus can agglutinate yeast fungus cells but after being exposured to mannose, agglutination to yeast fungus cells is lost or reduced. Results from this study we also got that inactivated and live bacteria that similarly adhere to intestinal epithelial cells.

Using of antiserum of hare resisting on chicken Lactobacillus adhesion receptor proteins of digestive tract epithelial cells,by agar diffusion experiment,discontinuation activity-PAGE electrophoresis and indirected ELISA technology,detected Lactobacillus adhesion receptor protein contents in crop and intestines of 1-40 days,healthy and coccidiosis chickens.The results suggested that 1 d chicken crop and intestines had Lactobacillus adhesion receptor protein,D450 nm was 0.236 and 0.176 respectively.Stabilization of the protein content was in 4-5 days,D450 nm was 0.231 and 0.166 respectively.Healthy chicken crop and intestines had more lactobacillus adhesion receptor proteins than those of chichens suffering from coccidiosis,D450 nm was 0.181 and 0.164 in crop;0.180 and 0.161 in intestines respectively.

为了比较猪、鼠、鸭C3d基因的不同,以便为下一步研究不同动物C3d作为分子佐剂核酸疫苗的效果,试验对3种基因进行克隆和序列分析。首先提取猪、鼠、鸭的肝脏总RNA,以RNA为模板,通过RT-PCR方法,分别扩增C3d分子的cDNA,再将3种动物的cDNA分别克隆到pMDl8-T载体中,然后转化BL21大肠杆菌,经酶切鉴定后进行序列测定,根据测序结果利用生物信息学工具软件对3种C3d进行生物学性质分析。结果发现,猪与鼠和鸭的C3d基因的同源性分别为81.9%和65.6%,鼠与鸭C3d基因的同源性为65.8%;鼠、猪的补体C3d分子与受体CR2结合的功能区有28个氨基酸,鸭有29个氨基酸。

This study was aimed to determine the effects of t lactobacillus probioticson AA broilersgrowth performance and immune function by testing the gain of weight,ratio of feed/gain,index of immune organs,antibody level,and quantity of lactobacillus,E.coliin caecal content 150 broilers were randomly assigned to 3 groups,group 1 were fed with 0.3% lactobacillus,group 2 with antibiotic,and group 3 were control.Broilers were vaccinated with NDV vaccine at the age of 7 days;tested the gain of weight and feed consummation at the end of every week;calculated the index of spleen,bursal,andthymuson the 21d and 35d;tested the antibody level with HI test(Haemagglutination inhibition) and determined the quantities of LactobacillusandE.coli in caecal content with micro-plate method respectively.The result shown that compared to antibiotic group and control,the group fed with 0.3% Lactobacillus probotics increasedthe weight by 15.55% and 8.57%(P<0.05),reduced theratio of feed/gain by 5.11% and 4.57% respectively.In Comparison with the control group probiotics also enhanced the index of spleen,bursal,thymus and 67.14%,16.47%,38.16% respectively,the antibody level by1.8log2 and 2 log2(P<0.05) on the 21d and 35d,while compared to antibiotic group the antibody level were increased by 1.4log2 and 1.75log2(P<0.05);on the 21d and 35d the group fed with 0.3% lactobacillusincreased the quantities of lactobacillusby 1.28log2and 0.27log2(P<0.05),reduced the E.coliby 1.88log2 and 0.84log2(P<0.05).This study provide theory and technology support for using lactobacillusprobiotics wisely.