Objective:To explore the difference in the proliferation, migration and ultraviolet radiation effect between double-head and unilateral pterygium fibroblasts (HPFs) cultured in vitro. Methods:Pterygium tissue was obtained from patients who underwent pterygium excision with conjunctival transposition from March 2017 to March 2018 in the Second Affiliated Hospital of Guangzhou Medical University.Nineteen cases with bilateral pterygium and 19 cases with unilateral pterygium were selected for this research.Twelve cases of normal conjunctival tissue were obtained from donor eyes.The fibroblasts were divided into HPFs-nasal (HPFs-N), HPFs-temporal (HPFs-T), unilateral HPFs and human conjunctiva fibroblasts (HCFs), which was taken from the nasal side of the bilateral pterygium, the temporal side of the bilateral pterygium, the unilateral pterygium and the normal conjunctiva, respectively.Human pterygium and normal conjunctival fibroblasts were isolated and cultured by tissue culture method.The fibroblasts were divided into an ultraviolet irradiation group and a normal light illumination group.The cell growth curve was detected by methyl thiazolyl tetrazolium (MTT) assay.The cell scratch healing rate was detected by the cell scratch test after 48 hours.The expression of α-smooth muscle actin αwas detected by immunofluorescence staining, and the number of positive cells in each group was compared.The fibroblasts cultured in vitro were irradiated with ultraviolet irradiation, and the cell scratch healing rate, growth curve and expression of α-SMA were observed.The study protocol was approved by the Ethics Committee of the Second Affiliated Hospital of Guangzhou Medical University.Written informed consent was obtained from each subject. Results:The pterygium and conjunctival fibroblasts were spindle shaped, and the growth rate was gradually increased from day 1 to day 7.The growth rate of HPFs-N was the fastest, and the growth rate of HCFs was the slowest.The growth rate of the four types of fibroblasts was significantly increased after exposure to ultraviolet.There were significant differences in the cell scratch healing rates and α-SMA positive cell expression rates among the four fibroblast types under different lighting conditions ( Fgroup=158.064, P<0.05; Fcell type=326.582, P<0.05. Fgroup=4.731, P<0.05; Fcell type=172.813, P<0.05), of which the scratches healing rate in the HPFs-N cells after 48 hours under the normal light conditions was (79.67±0.86)%, which was significantly higher than HPFs-T ([54.04±0.33]%), unilateral HPFs ([64.12±0.21]%) and HCFs ([58.86±0.41]%), the α-SMA positive cell expression rate in the HPFs-N cells after 48 hours under the normal light conditions was (28.87±1.02)%, which was significantly higher than that in HPFs-T ([13.67±0.23]%), unilateral HPFs ([20.35±1.72]%) and HCFs ([5.12±0.45]%) (all at P<0.05); the cell scratch healing rates and α-SMA positive cell expression rates were significantly increased in the ultraviolet irradiation group than those in the normal light illumination group (all at P<0.05). Conclusions:The nasal side of double-head pterygium fibroblasts is more proliferous and more migratory than that of the unilateral pterygium, the expression of α-SMA is also increased, which can be further enhanced by ultraviolet irradiation.

目的 探讨翼状胬肉与睑裂斑的病理改变差异和磷酸化细胞外信号调节激酶(p-ERK)及含IQ模序的GTP酶活化蛋白1(IQGAP1)的表达,比较其在翼状胬肉、睑裂斑中的差异.方法 8例翼状胬肉、睑裂斑病理及正常结膜组织,HE染色显示组织结构,免疫组化检测各组织中p-ERK和IQGAP1的表达.结果 上皮层数比较:翼状胬肉>睑裂斑>结膜.血管数量比较:翼状胬肉>睑裂斑>结膜.翼状胬肉、睑裂斑组织中p-ERK、IQGAP1表达水平明显高于正常结膜.相关分析显示翼状胬肉中p-ERK与IQGAP1表达呈正相关.结论 翼状胬肉和睑裂斑中的p-ERK和IQGAP1表达增加,共同参与翼状胬肉、睑裂斑的发病过程.翼状胬肉和睑裂斑的发病机制可能有一定相似性.

Objective To investigate the efficacy of phacoemulsification combined with negative power intraocular lens implantation in the management of cataract with high myopia.Methods Clinical data including outcome measures and complications of 75 patients (116 eyes) with cataract and super high myopia who underwent phacoemulsification combined with negative power intraocular lens implantation in the Department of Ophthalmology, the Second People′s Hospital of Futian District in Shenzhen City between January,2012 and January,2014 were retrospectively analyzed by t-test and Kruskal-Wallis H test.Results The average preoperative diopter was -8.43 ±0.71 D/13.43 ±0.71 D.The combined treatment robustly reduced the average diopter (-8.35±0.05 D/-2.55±0.87 D,t=68.61,P <0.05/0.01).The visual acuity was 0-0.1 in 32 cases (49 eyes, 42.24%),0.2-0.5 in 43 cases (67 eyes,57.76%) before treatment and was 0-0.1 in 8 cases (13 eyes, 11.21%),0.2-0.5 in 10 cases (15 eyes,34.13%),0.6-1.0 in 20 cases (31 eyes, 26.72%),and 1.1-1.5 in 37 cases (57 eyes,49.14%).The difference before and after treatments was statistically significant (Hc=5.81,P<0.05).After surgery,capsular rupture was observed in 1 case (2 eyes),dry eye in 3 cases (6 eyes),posterior capsule opacification in 3 cases (5 eyes),descemet membrane detachment in 1 case (1 eye) and corneal edema in 9 cases (14 eyes).Neither retinal detachment nor elevated intraocular pressure occurred in any of the cases.Conclusion Phacoemulsification combined with negative power intraocular lens implantation is effective in treating super high myopia patients complicated by cataract.

Objective To investigate the morphological characteristics of pathological myopia using fundus fluores -cein angiography ( FFA) , indocyanine green angiography ( ICGA) and optical coherence tomography ( OCT) .Methods We studied 28 cases (31 eyes) of pathological myopia with macular hemorrhage .FFA, ICGA, and OCT examinations were performed to characterize the morphological changes in these patients .Results Uncomplicated macular hemorrhage was i-dentified in 15 eyes (48.4%).Among these eyes, FFA showed masked fluorescein in the bleeding region .Striated bands of fluorescein could be observed in 8 eyes (53.5%).ICGA showed similar masks at initial phase and fluorescein bands at later phases.OCT revealed high reflection in neurosensory retina (9 eyes, 60%) or in RPE (4 eyes, 26.7%).There was no retina edema.Choroid appeared to be continuous .In 2 eyes, OCT were completely normal.Another group of eyes ex-hibited choroidal neovascularization (CNV;n=16, 51.6%).FFA identified overt CNV in 14 eyes (87.5%) and occult CNV in 2 eyes (12.5%), while ICGA detected scattered CNV in 14 eyes (87.5%) and in two eyes there was no fluores-cein leakage .OCT showed discontinuation and spindle or round shaped high reflection within RPE in 14 eyes.In 2 other eyes, RPE discontinuation was also found along with irregular thickened RPE and neurosensory retina edema .Conclusion Pathologicsl myopia can present with two different types of pathological changes , uncomplicated macular hemorrhage and choroidal neovascularization .Different morphological characters can be identified through FFA , ICGA and OCT examina-tions.

Objective To evaluate the clinic effect of topography guided (Optimized Refractive Keratectomy-Custom Ablation Manager ,ORK-CAM) laser in situ keratomileusis (LASIK) for high astigmatism treatment. Methods Sixty-seven cases (89 eyes) with high corneal astigmatism were randomly divided into ORK-CAM group (39 cases, 53 eyes) which accepted Schwind Esiris ORK-CAM topography-guided LASIK and LASIK group (28 cases, 36 eyes) which accepted standard small optical zones flying-spot ablation LASIK. The preoperative average subjective spherical refraction was (-4.23±1.31)D,(-4.42±1.58)D,and cylindrical refraction was (-2.50±0.52)D, (-2.49±0.47)D in ORK-CAM group and LASIK group respectively(P0.05). All cases were followed up for 6 months. Results The postoperative subjective refraction was (-0.14±0.76) D and (-0.12±0.82) D (P0.05), and cylindrical refraction was (-0.51±0.63) D and (-0.98±1.21) D (P0.01) in ORK-CAM group and LASIK group respectively. Eighty-one percent of the ORK-CAM group achieved 1.0 or better visual acuity while that in the comparative group was 80%. There was no significant difference of postoperative visual acuity between two groups (P0.05). The coma was (0.33±0.15) μm and (0.42±0.27) μm (P0.05), the spherical aberration was (0.51±0.24) μm and (0.92±0.32) μm (P0.01), and the Q-value was 0.55±0.34 and 1.17±1.23(P0.001) in ORK-CAM group and LASIK group respectively. One month after operation, the contrast sensitivity of ORK-CAM group returned to their pre-operative level, and that of LASIK group returned to preoperative level at the third months after operation. Conclusions ORK-CAM topography guided LASIK for high corneal astigmatism treatment is superior to conventional LASIK.

Objective To evaluate the effect of phacoemulsification with different transparent corneal incisions and foldable posterior chamber intraocular lens implantation on corneal astigmatism.Methods Eighty-one eyes of 65 patients with age-related cataract were divided into three groups randomly.The transparent corneal oblique incision at temporal side was performed in 28 eyes of group A,transparent corneal oblique incision at 11 O’clock in 26 eyes of group B,and transparent corneal tunnel incision at 11 O’clock in 27 eyes of group C.The length of all incisions was 3.2 millimeter.The conventional phacoemulsification was operated by the same ophthalmologist.At 1 day before operation and 1 day,7 days,1 month and 3 months after operation,the examination of visual acuity,slit lamp microscope and retinoscopy were performed,the acquired indexs were statistic analyzed.Results The visual acuity in each group after surgery was all higher than that before surgery;At 1 day and 1 week after surgery,there were significant difference in statistics between group A and group B,C respectively,but there was no significant difference between group B and group C;At 1 and 3 months after surgery,there was no significant difference in visual acuity among three groups.The astigmatism in group A were lower than that in group B and group C,there were significant difference (P0.05),but there was no significant difference between group B and group C (P0.05).Conclusion The transparent corneal incision at temporal side is convenient to operate,has the least effect on corneal astigmatism and visual acuity can recover rapidly,so it is the most ideal incision site.Oblique and tunnel incisions has no statistical difference,but the operation of oblique incision is more convenient than that of tunnel incision.

Objective To study the cosmetic effect of radical pterygium removal.Methods The total of 206 patients(263 eyes) with pterygium had been randomly allocated into two groups:group A and group B,who had all received pterygium excision and conjunctival rotation autograft in hospitalization.92 patients(120 eyes) were in group A,who had all received radical pterygium removal,namely,pterygium excision and conjunctival rotation autograft combined with extended fascia removal and the residual conjunctive removed as possible;114 patients(143 eyes) were in group B,who had only received pterygium excision and conjunctival rotation autograft.All the patients of the two groups had been followed up for 24 months,and the cosmetic effect was observed by counting the rate of poor cosmetic eyes including recurrence and regeneration.Results Group A had 17 eyes with poor cosmesis(at recurrence of 14.17%) and group B had 87 eyes with poor cosmesis(at recurrence of 60.84%) respectively.The two groups had significant difference(χ2 = 59.45,P 0.005) by statistical comparative analysis.Conclusion Radical pterygium removal is more effective than pterygium excision and conjunctival rotation autograft for cosmesis.

Objective To study the prevent methods of pterygium recurrence after operation.Methods A total of 114 patients (155 eyes) with pterygium were randomly allocated into two groups,all of the patients received pterygium excision and eonjunctival rotation autogratt hospitalized and analyzed retrospectively.There were 45 patients (62 eyes) in group A,all of the patients received radical pterygium removal,namely,pterygium excision and conjunctival rotation autograft combined with extended fascia removal and the residual conjunctive removed as possible.There were 69 patients (93 eyes) in group B,all of the eyes only received pterygium excision and conjunctival rotation autograft.All the patients of the two groups were followed up for 12 to 69 months with an average of 28.5 months,and observed if there were recurrences.Results There was 1 eye found recurrent pterygium in group A while 14 eyes in group B.The recurrent rates were 1.6% and 15.1% respectively.There were statistical significant differences between two groups analyzed by x~2 examination (x~2=7.49,P＜0.01).Conclusions Radical pterygium removal is effective for preventing pterygium recurrence.

目的 探讨肿瘤坏死因子TNFα-1031基因多态性与葡萄膜炎的关系.方法 运用多聚酶链反应技术检测33例葡萄膜炎及119例正常人肿瘤坏死因子TNFα-1031G/A基因多态性.结果 葡萄膜炎组肿瘤坏死因子TNFα-1031 T/T、 T/C、C/C表型频率分别为:0.5455、0.4545、0,对照组分别为0.5799、0.4117、0.0084;葡萄膜炎组TNFα-1031 T、TNFα-1031 C基因频率分别为:0.7727、0.2273,对照组分别为0.7857、0.2143, 肿瘤坏死因子TNFα-1031G/A各种基因型频率在葡萄膜炎组与正常对照组之间的差异无显著性(P>0.05).结论 肿瘤坏死因子TNFα-1031G/A基因多态性与葡萄膜炎无明显相关.

目的比较白内障超声乳化吸除(phacoemulsification,PHACO)联合房角粘连分离术治疗不同房角粘连关闭程度的原发性闭角型青光眼(primary angle-closure glaucoma,PACG)的临床效果。方法 61例65眼原发性闭角型青光眼合并白内障患者,分成A组(房角粘连关闭≤180°)42例43眼;B组(房角粘连关闭>180°)19例22眼,均施行白内障超声乳化吸除联合房角粘连分离术,观察两组手术后1、3、6个月的房角、眼压、视力和视野的变化。结果术前眼压:A组为(19.12±4.73)mmHg(1 mmHg=0.133 kPa);B组为(23.32±6.31)mmHg。术后1、3和6个月的复查中,眼压正常者(不用降眼压药):A组为97.7%、97.7%和95.3%;B组为77.3%、72.7%和63.6%。房角改变:A组3次复查全部开放;B组则分别为77.3%、68.2%、68.2%。最佳矫正视力≥0.5者:A组为90.7%、93.0%、93.0%,B组为77.3%、72.7%、63.6%。A组视野术后扩大者均为100%,B组为81.8%、72.7%、63.6%。各观察指标中A组优于B组(P<0.05)。结论对于合并有白内障的PACG患者,施行白内障超声乳化吸除联合房角粘连分离术可以使大部分患者的房角重新开放,但对于部分房角粘连关闭>180°者效果欠佳,需要定期追踪观察。

Objective To investigate the relationship between tumor necrosis factor(TNF)-238G/A polymorphism and endometriosis. Methods The polymorphism of TNF α-238G/A gene was screened by PCR-RELP. Results The gene frequency of TNFα-238G/A in endometriosis group was as followed:TNF α-238G/A,G/G,A/A genotype frequency:0.9300,0.070,0; TNFα-238 G,TNFα-238 A genefrequency:0.9650,0.0350. The gene frequency of TNFα-238G/A in control group was as followed:TNFα-238G/G,G/A,A/A genotype frequency:0.9500,0.050,0,TNFα-238 G,TNFα-238 A genefrequency:0.9750,0.0250,There were no significant differences in the frequencies of TNFα-238G/A gene between the patients and control group (P0.05). Conclusion No evident association was found between TNFα-238G/A polymorphism and endometriosis.

目的探讨兔眼不同切削量LASIK角膜扩张的变化,拟有效、合理地建立医源性角膜扩张的动物模型。方法选择新西兰白兔18只,随机分成A、B、C 3组,每组6只白兔选择单眼进行LASIK手术,在角膜基质层进行PTK激光切屑,切屑光斑6 mm,角膜基质层剩余厚度A、B、C组分别为250μm、150μm、100μm,观察各组手术前后角膜地形图的变化。结果A、B、C组术后与术前角膜地形图差值比较,术后1周分别为(-2.12±1.8)D、(2.98±0.47)D、(7.6±0.45)D;术后1个月分别为(-2.25±1.38)D、(3.13±1.19)D、(8.07±0.88)D。结论对新西兰白兔施行LASIK术中角膜基质层常规保留250μm,在观察期内未发生角膜扩张;剩余角膜基质层厚度为150μm和100μm均可引起角膜扩张,切削量越多则角膜扩张越明显,因此可以根据研究需要制作不同基质床厚度的PLK模型。

目的 研究LASIK术中应用角膜微型板层刀的全新刀片,通过高压消毒处理后制作较薄角膜瓣的效果.方法 选择双眼屈光度相近、同时进行双眼LASIK术的患者63例(126眼),术中使用M2的90微型刀头制作角膜瓣,右眼使用全新刀片,左眼使用连续高压消毒3次后的新刀片.术中用A超角膜测厚仪进行角膜厚度测量,计算角膜瓣的厚度.术后观察3个月的视力、屈光度、对比敏感度和角膜地形图(角膜像差)的变化.结果 右眼和左眼术前屈光度(等效球镜度)分别为:-2.25~-9.50.D,平均(-5.28±1.73) D和-2.00~-9.88.D,平均(-5.51±1.62) D(P>0.05);角膜瓣厚度为:(133.9±12.3) μm和(114.7±13.1) μm(P＜0.05);术后屈光度分别为: (-0.12±0.23) D和(-0.15±0.12) D(P>0.05);裸眼视力分别为:1.05±0.21和1.05±0.19(P>0.05);高阶像差均方根值(RMS)分别为:(0.68±0.12) μm和(0.66±0.13) μm(P>0.05);对比敏感度:在3.0.c/d、6.0.c/d、12.0.c/d、18.0.c/d空间频率的比较中两组数值相近(P>0.05).结论 应用经过高压处理后的新刀片可以制作出优质的、较薄的角膜瓣.

Objective To evaluate the clinic effects of phacoemulsification in early primary angleclosure glaucoma with cataract. Methods 48 cases ( 52 eyes ) with early primary angle-closure glaucoma and cataract were received phacoemulsification. The intraocular pressure ( IOP ), anterior chamber angle and visual acuity were examined before and after operation. All were followed up 6 to 28 months. Results The mean postoperative IOP ( 12.34±3.76 mmHg, 1 mmHg=0.133kpa ) was lower than the mean preoperative IOP ( 19.24±3.76 mmHg ). The closed anterior chamber angles were opened, the visual acuity improved significantly. Conclusions In cases of early primary angle-closure glaucoma with cataract, the anterior chamber angle became wider after phacoemulsification. The visual acuity improved and IOP can be controlled after the operation.

OBJECTIVE:To study the microsurgical procedures for the treatment of large primary pterygium and their therapeutic effects. To observe the recurrence rate, the changes of visual acuity after microsurgery and the pathological relationships between pterygium and cornea/sclera under the surgical microscope.METHODS:Forty-six eyes of forty-one patients with pterygium which invading the cornea over the pupil border were included. Pterygium was dissected by various methods under surgical microscope. The pathological relationship between the pterygium and cornea/sclera was observed. The lengths of the pterygium head and its three parts were measured. Degenerative Tenon's capsule was removed totally and the wound was covered by rotated conjunctival flaps. These patients were followed-up for 12.0 - 50.2 months (median: 22.4 months). Changes in visual acuity and recurrence rate after operation were observed.RESULTS:The average length of the total pterygium heads was (6.3 +/- 0.4) mm. The head was divided into three parts: the apical, loose and adhesive parts. The apical part was located at the top of the pterygium head with a length of (1.7 +/- 0.4) mm. The tissue of apical part was compact, hard, translucent and adhered to the cornea tissue. The adhesive part was a band in front of the anterior border of the limbus and paralleled to the limbus. The width of adhesive part was (0.9 +/- 0.1) mm and was tightly adhered to the cornea. The loose part lied between the apical and the adhesive part. The length of which was (3.6 +/- 0.4) mm and could be separated from the cornea easily. The neck and the body parts of pterygium could be separated easily from the limbus and sclera. Non-corrected visual acuity averaged 0.3 (ranged from finger count to 0.7) before the operation and averaged 0.7 (ranged from finger count to 1.5) 1 month postoperatively (Wilcoxon signed rank test u = 5.435, P < 0.01). Pterygium relapsed in 5 eyes with a recurrence rate of 11% (5/46).CONCLUSIONS:There is a regular pathological relationship between the pterygium and the cornea/sclera under surgical microscope, which is fundamental for the microsurgery of the pterygium. Extensively degenerated Tenon's capsule should be removed totally and the defect should be covered by rotated conjunctival flaps. The recurrence rate is low and the visual acuity increases significantly after the operation.

Objective To investigate the methods of phacoemulsification and intraocular lens implantation(PHA +IOL)for cataract with small pupil,synechia and atouic iris(SPSAI)after glaucoma trabeculectomy,which is simple, save and faster recovery of visual acuity.Methods Fifty eyes of forty-five patients with SPSAI cataract after trabeculecto- my were divided into A and B groups according their operating methods and analyzed retrospectively.There were 31 eyes of 27 patients in group A,the treatment method of SPSAI was small segmental iridectomy and coreoplasty(SSIC).There were 19 eyes of 18 patients in group B,the treatment method of SPSAI was stretching and dilating the pupil(SDP)to 4～5mm. The visual acuity,the pupil' s situations were observed at the first,seventh and thirtieth days after operation.The operative procedures were recorded.Results The different of visual acuity of the two groups was significant statistically at the first day after operation,namely the recovery of the visual acuity of the patients of group A was faster than that of group B(u= 2.38,P＜0.05).Compared with SDP,SSIC had no laceration of posterior lens membrane,much less bleeding,shorter operating time and better recovery of the pupils.Conclusion PHA+IOL for the cataract with SPSAI after glancoma trab- eculectomy,compared with SDP,SSIC made the operation simple and save,the recovery of the visual acuity was faster.

Objective To evaluate and compare central corneal thickness in long term soft contactlens wear and long term rigid gas-permeable contact lens (RGP) wear in normal eyes. Methods 500 normalcases (918 eyes),200 long term soft contact lens wear cases(386 eyes) and 100 long term RGP wear cases(195eyes) were enrolled in this study.Ultrasound pachymetry was used to measure central corneal thickness for eacheye. Results The central corneal thickness was (512.31±21.73)μm and (528.59±23.37)μm in long termsoft contact lens wear and normal eyes,respectively (P<0.05). The central corneal thickness was (515.03±22.97)μm and (528.59±23.37)μm in long term RGP wear and normal eyes, respectively (P<0.05). There were nostatistical differences (P>0.05) in central corneal thickness between long term soft contact lens wear and longterm RGP wear. Conclusion The central corneal thickness was significantly thinner in long term soft contactlens wear and long term RGP wear than in normal eyes .

Objective:To observe the changes in the function of tear film after laser in situ keratomileusis(LASIK) for myopia.Methods: One hundred myopic patients (186 eyes) treated with LASIK were evaluated for tear flow(Schirmer I test),tear break-up time(BUT) tests and fluorescein staining scores before operation and at l week,l month and 3 month intervals after the operation.Results:(1) the mean values of Schirmer I were(15.89±6.70) mm preoperatively;(12.08±7.30)mm,(10.43±5.52)mm,(11.56±6.82)mm at l week,l month and 3 months postoperatively(P<0.05).(2) The mean BUT were(12.83±5.04) seconds preoperatively,(9.07±6.40) seconds,(8.74±7.56) seconds and(10.23±5.76) seconds at l week,l month and 3 months postoperatively(P<0.05).(3) The FL staining was intensified at l week postoperatively as compared with before operation.Conclusion: LASIK caused a decrease in tear film stability and tear secretion early stage after operation,probably due to decreased corneal sensation after LASIK.

[Objective] To observe the effect of the LASIK for myopia. [Methods] According to the refractive degrees before LASIK surgery, 400 cases (797 eyes) were divided into three groups: A Group: 456 eyes, -1.00～-6.00D (Average:-4.25D±1.23D); B Group: 309eyes, -6.25～-12.00D (Average-7.86D±1.45D); C Group: 32eyes, -12.25～-24.75 (Average -15.82D±3.05D). Record the Vision, Tonometry, Refractive, Forepart eyeball, Eyeground, Corneal thickness and Corneal topography. [Results] Compared with three groups. Result: Vision after LASIK ≥1.0: A Group 91.01%, B Group76.05, C Group15.63; χ2=129.00, P =0.000, compared between three groups, There was obvious difference. Residue diopter ≤±0.5D: A Group75.22, B Group 63.43, C Group18.75; χ2=49.82, P =0.000, It was obvious different comparing with three groups. [Conclusion] Lasik Surgery is good for myopia ,especially for the eyes vision under 12.00D.

目的利用彩色多普勒血流显像技术观察全身或局部应用葛根素治疗青光眼后眼动脉(OA)、睫状后短动脉(SPCA)及视网膜中央动脉(CRA)的血流动力学改变。方法将原发性开角型青光眼或慢性闭角型青光眼共61例(122只眼)随机分成A组(全身用药组)30例(60只眼)和B组(局部用药组)31例(62只眼)。A组应用葛根素全身静脉滴注;B组应用1%葛根素(达靓)眼液点眼治疗。用药时间均为14d。治疗前后进行彩色多普勒显像(CDFI)检查,测定血流参数为收缩期峰值血流速度(PSV)、舒张末期血流速度(EDV)和阻力指数(RI)。结果两组治疗前各参数比较差异无显著性(P>0.05),治疗后除B组OA血流改变不明显外,两组其他被观察的动脉血流供应增加,血流阻力指数降低;A组改变更明显(P<0.01)。结论全身或局部应用葛根素治疗青光眼均有改善血流供应的作用,在相同用药时间里全身用药效果更好。