Objective To explore the distribution characteristics of fungi from soil of mangrove in Hainan.Methods Isolation and culture fungi from mangrove soil samples on the PDA medium,identified and explore the taxonomic status of strains by means of observation on morphology and cultural characteristics,combined with rDNA ITS sequences of squence and phylogenetic analysis.Results 325 fungus strains attained from mangrove soil samples were distributed in 14 genera,counting Penicillium sp.29.84%,Trichoderma sp.22.46%,Aspergillus sp.20.00%,Paecilomyces sp.6.46%,Cladosporium sp.4.31%.Conclusion The fungi resources in mangrove are abundant in Hainan.Penicillium sp.,Trichoderma sp.and Aspergillus sp.were the main genera.