Objective]To construct the animal plateau frostbite (PF)models .[Methods]32 rats were randomly divided into in blank control group,Ⅰ plateau frostbite group,Ⅱ plateau frostbite group and Ⅲplateau frostbite group,8 rats in each group.A low-pressure composite cabin was used to simulate plateau en-vironment .After 2 weeks of hypoxia in rats,I to III degree plateau frostbite models were constructed by con-trolling the liquid nitrogen freezing time,and the control group was not treated.48 h after modeling in each group,ultrasonic imaging was conducted by the intravenous Sovovue on the frozen part of the body for blood microcirculation imaging,and the frostbite parts of tissues of rats in each group were stained by HE staining. The pathological changes of different degree frostbitten tissue were observed.[Results]Blocked blood flow of the three groups was significantly increased with the increased degree of frostbite on plateau frozen tissue (P<0.05);Ⅰ plateau frozen tissue shew epidermis injury with leakage of red blood cell;Ⅱ plateau frostbite in-jury was suffered to the deep dermis and muscle surface;Ⅲ degree of plateau frostbite destroyed the almost all the muscle layer (P <0.05).[Conclusion]Ⅰ~Ⅲ plateau frostbite models can be directly constructed by con-trolling the liquid nitrogen freezing time .This approach has strong operability.

Objective To reproduce the model of plateau hypoxia in different degrees in pregnant sheep by using hypobaric chamber for the evaluation of oxygenation status and oxidative damage in these models. Methods Twenty-four pregnant sheep conceived by successful artificial insemination were randomly divided into three groups (8 each): low altitude control group (LAC), mild hypoxia exposure group (MHE) and severe hypoxia exposure group (SHE). Sheep in the latter two groups were fed in hypobaric chamber, and the models were reproduced by adjusting the temperature, humidity, pressure and oxygen content simulating environment of different altitudes of northwest China. The degree of oxygenation, vital signs, behavior pattern and oxidative damage biomarkers were dynamically determined before modeling and 7, 15, 30, 90, 120 days after modeling, respectively. Results Thirty and 90 days after modeling, the arterial blood gas parameters of sheep in MHE and SHE group met the diagnostic criteria. Ninety days after modeling, the respiratory frequency decreased (P<0.05), the heart rate increased (P<0.05), and lassitude were found in MHE and SHE groups than in LAC group; the consumption of feeding decreased in SHE group than in MHE group (P<0.05). One hundred and twenty days after modeling, the CO and MDA increased significantly in MHE and SHE groups than those in LAC group (P<0.05), also in SHE group compared with that in MHE group (P<0.05). Conclusion Different degrees of hypoxia models of pregnant sheep have been successfully reproduced by using hypobaric chamber, and the results showed that the hypoxic environment in high altitude regions may cause oxidative stress injury of gestational sheep. DOI: 10.11855/j.issn.0577-7402.2015.05.05

目的 探索载舒尼替尼的新型多聚体型微泡抑制人肾癌GRC-1细胞增殖及诱导凋亡的作用.方法 体外培养人肾癌GRC-1细胞,将其分为空白对照组、舒尼替尼组、新型多聚体型微泡联合超声组(pMB+ US组)、载舒尼替尼的新型多聚体型微泡不联合超声组(pMBS-US组)、载舒尼替尼的新型多聚体型微泡联合超声组(pMBS+ US组).舒尼替尼组、pMBS-US组、pMBS+ US组分别以0.01、0.10、1.00 μg/ml舒尼替尼给予处理,于不同时间以MTT法观察不同处理组细胞生存率,检测细胞凋亡,并用HE染色法及DAPI荧光染色法观察凋亡细胞形态学改变.结果 pMBS+ US组较之其余各组对肿瘤细胞有更为明显的抑制增殖及诱导凋亡效应(P＜0.01).结论 载舒尼替尼的新型多聚体型微泡在超声作用下能显著促进药物与肿瘤细胞的作用,为靶向持续药物运输提供了可能.