目的 了解唐山市丰南区22家预防接种门诊物体表面及接种人员手部清洁度状况,促进接种门诊对清洁消毒工作的重视.方法 采用ATP生物荧光检测仪及其配套试剂,对接种过程中频繁接触的接种台面、冰箱开启时手接触部位以及接种人员手部进行检测,评估其清洁度,利用SPSS 20.0软件进行统计分析.结果 二级医院组与基层医疗卫生机构组预防接种门诊接种台面检测合格率分别为66.67％和68.48％,差异无统计学意义(x2=0.027,P=0.987);冰箱开启时手接触部位检测合格率分别为33.33％和54.76％,差异无统计学意义(x2=2.842,P=0.242);接种人员手部检测合格率分别为41.67％和50.00％,差异无统计学意义(x2=0.383,P=0.536).结论 各医疗卫生机构的预防接种门诊对于消毒工作的重视程度需亟待提高.

Objective To evaluate the effect of school lunch nutrition comprehensive intervention for families of students.Methods A cluster sampling method,selected three public elementary schools located in Fengnan District of sixth grade students will study subjects were randomly divided into three groups,which provide a comprehensive intervention group school lunch nutrition comprehensive intervention;group to provide nutritious meals nutritious lunch,but not comprehensive intervention;pure control group did not provide a nutritious lunch is not comprehensive intervention.A year later statistical analysis of families of students cognitive,behavioral,body mass index (BMI) change,the evaluation of the implementation results.Results Comprehensive intervention group students after lunch importance (x2 =0.25,P ＞ 0.05),(x2 =2.00,P ＞ 0.05),nutritional knowledge interest(x2 =2.26,P ＞ 0.05),(x2 =0.04,P ＞ 0.05),time to eat lunch (x2 =0.08,P ＞ 0.05),(x2 =0.03,P ＞ 0.05) and other aspects of the other two groups showed no significant difference;comprehensive intervention group after the intervention in a completely correct perception meal pyramid(x2 =119.8,P ＜0.05),(x2 =143.18,P ＜ 0.05),picky eaters partial eclipse habits(x2 =619.95,P ＜ 0.05),(x2 =692.22,P ＜ 0.05),reduce the number of underweight(x2 =28.849,P ＜ 0.05),(x2 =36.297,P ＜ 0.05) and other aspects of the other two groups was statistically significant.Conclusion The implementation of a comprehensive intervention can better improve students' cognitive and behavioral family nutrition,increase physical fitness,promotion of students having lunch nutrition.

Spoolbase是深水硬质海底管线R-Lay铺设配套的陆地海管预制基地,通过分析Spoolbase发展现状,已发展了岸基固定式与可移动便携式Spoolbase,且全球深水油气田开发附近只有南海尚未布设岸基固定式Spoolbase;并通过分析Spoolbase主要生产工艺及我国深水油气田开发市场,为我国Spoolbase投资建设提供一定的借鉴.

Illustrated by the case of MOCK-UP project,built by COOEC(Qingdao) and Kvaerner,to analyze the gap between China's marine engineering enterprise and first-class international corporation related and some improvement suggestions are put forward.

目的观察D-核糖对高脂喂饲的C57BL/6小鼠血糖、血脂及胰岛素等的影响,探讨D-核糖对改善高脂膳食引起的糖耐量异常和胰岛素抵抗的可能性。方法 8 w龄C57BL/6雄性小鼠36只,随机分成正常对照、高脂对照、低剂量的D-核糖+高脂组(高脂+2.5%核糖)和高剂量的D-核糖+高脂组(高脂+5%核糖),喂饲12 w,记录摄食量和体重变化。11w进行葡萄糖耐量试验(OGTT),并计算葡萄糖曲线下面积(AUC)。干预12w处死,进行血生化指标及胰岛素检测。结果高脂+5%核糖组小鼠的血糖水平低于高脂对照组(P 0.05);高脂+5%核糖组血清低密度脂蛋白胆固醇(LDL-C)、血清甘油三酯(TG)及游离脂肪酸(FFA)低于高脂对照组(P<0.05)。结论 D-核糖可以改善高脂饲料喂饲小鼠的糖耐量异常和胰岛素抵抗。[营养学报,2013,35(2):142-145]

Objective To study the regulation of D-ribose on blood glucose,lipids and insulin in C57BL / 6 mice fed a high-fat diet,and to explore the effect of D-ribose on impaired glucose tolerance and insulin resistance.Methods Thirty-six C57BL / 6 mice were assigned to four groups,namely normal control,high-fat control,low doses of D-ribose + high-fat diet(high-fat +2.5% ribose) and high dose of D-ribose + high-fat diet group(high-fat +5% ribose).After 11 weeks,oral glucose tolerance test(OGTT) was performed and glucose area under the curve(AUC) was calculated.Experimental period lasted 12 weeks.The detection of blood biochemical parameters and insulin were conducted after the mice were killed.Results Levels of fasting glucose were lower in the mice fed the high-fat diet+ 5%D-ribose than in the mice fed the high-fat diet(P0.05).The fasting serum insulin concentrations and HOMA-IR were signi?cantly lower in the high-fat diet+ 2.5% D-ribose and high-fat diet+ 5% D-ribose groups than in high-fat control group(P0.01).The levels of serum cholesterol(CHO) and high-density lipoprotein cholesterol(HDL-C) showed no significant difference among the three groups fed the high-fat diets(P0.05).Serum triglyceride(TG),low density lipoprotein cholesterol(LDL-C) and free fatty acids(FFA) levels were lower in the high-fat diet+ 5% D-ribose groups than in the high-fat control group(P0.05).Conclusion These results suggest that d-ribose exerts a preventive effect on impaired glucose tolerance or insulin resistance induced by the high fat diet.[ACTA NUTRIMENTA SINICA,2013,35(2):142-145]

OBJECTIVE:Study the effects of β-glucan in highland barley on blood glucose and serum lipid in high fat diet induced C57 mouse. METHODS:Using table of random number, 40 male C57BL/6 mice were randomly divided into 4 groups (10 mice in each group) by weight: high dosage group (4% β-glucan and high fat diet), low dosage group (2% β-glucan and high fat diet), high fat diet group and normal control group. Food-intake and body weight of C57 mouse were observed. Glucose tolerance tests and examinations of fasting blood glucose were performed at the end of 11 weeks of intervention. Mice were sacrificed after 12 wk of treatment, and serum specimens were obtained to test relevant biochemical indicators. RESULTS:After 12 weeks raise, among high dosage group, low dosage group, high fat diet group and normal control group, the weight was (32.8 ± 1.5), (40.4 ± 1.9), (40.7 ± 2.1) and (33.5 ± 1.3) g, respectively (F = 55.26, P < 0.05); average food intake was (3.48 ± 0.56), (3.69 ± 0.76), (3.66 ± 0.81) and (3.54 ± 0.61) g/d respectively (F = 0.26, P > 0.05); fasting blood-glucose was (5.29 ± 1.59), (6.13 ± 1.75), (7.63 ± 1.09) and (4.24 ± 0.98) mmol/L respectively (F = 9.54, P < 0.01); serum insulin level was (1.97 ± 0.10), (2.44 ± 0.24), (3.02 ± 0.36) and (1.48 ± 0.28) ng/ml respectively (F = 47.58, P < 0.01); the area under blood glucose concentration curve was (25.81 ± 1.44), (30.42 ± 2.01), (35.17 ± 1.20) and (21.03 ± 1.24) mmol×L(-1)×h(-1), respectively (F = 64.98, P < 0.05); insulin resistance index was (9.84 ± 3.78), (13.69 ± 4.48), (21.54 ± 3.27) and (5.81 ± 1.59) respectively (F = 30.18, P < 0.01); serum total cholesterol (TC) level was (4.05 ± 0.88), (4.30 ± 0.48), (4.73 ± 0.66) and (3.37 ± 0.40) mmol/L respectively (F = 6.70, P < 0.01); serum triglyceride (TG) level was (0.90 ± 0.09), (0.98 ± 0.09), (1.05 ± 0.06) and (0.76 ± 0.26) mmol/L respectively (F = 6.75, P < 0.01); serum high-density lipoprotein cholesterol (HDL-C) level was (2.91 ± 0.59), (3.34 ± 0.46), (4.89 ± 0.42) and (3.24 ± 0.37) mmol/L respectively (F = 31.73, P < 0.01); serum low-density lipoprotein cholesterol (LDL-C) level was (0.25 ± 0.15), (0.42 ± 0.19), (0.72 ± 0.12) and (0.32 ± 0.11) mmol/L, respectively (F = 17.27, P < 0.01); free fatty acids (FFA) level was (1.06 ± 0.03), (1.05 ± 0.05), (1.18 ± 0.32) and (1.04 ± 0.02) mmol/L, respectively (F = 1.36, P > 0.05); HDL-C/LDL-C was (13.77 ± 5.51), (9.11 ± 3.53), (7.04 ± 1.65) and (11.21 ± 3.31), respectively (F = 5.24, P < 0.01). CONCLUSION:The β-glucan in highland barley reduced the serum glucose and serum lipid, as well as insulin resistance and the risk of arterial sclerosis in high-fat induced C57 mouse.

The horizontal pressure of barge to the front skid way block is huge,the barge got up and down movements caused by wind,sea wave,flow and ship wave,which cause a huge frictional force between barge and skid way block.As a result,the reinforced concrete skid way block was destroyed by shear stress.Meanwhile,the connection strength between the steel structural anti-collision block and the the reinforced concrete skidway block is not enough,the whole stiffness of the reinforced concrete skid way block decreases.So it is necessary to improve the front skid way block of to meet the requirements of work.

Adipocyte differentiation and adipogenesis are closely related to obesity and obesity-induced metabolic disorders. The calcium-sensing receptor (CaSR) has been reported to play an antilipolytic role in human adipocyte and regulate cell differentiation in many tissues. However, the effects of CaSR on adipocyte differentiation and adipogenesis have not been clarified. In the study, we observed that activation of CaSR significantly promoted adipocyte differentiation and adipogenesis in human SW872 adipocytes. Gene expression analysis revealed that the CaSR activation increased the transcription factor proliferator-activated receptor γ (PPARγ) and its downstream genes including CCAAT element binding protein α (C/EBPα), adipose fatty acid-binding protein (aP2), and lipoprotein lipase. The activity of glycerol-3-phosphate dehydrogenase was also increased after the stimulation of CaSR. In addition, levels of cyclic AMP and calcium which have been shown to regulate PPARγ gene expression were significantly affected by the activation of CaSR. These effects could be suppressed by CaSR small interfering RNA (CaSR-siRNA). In conclusion, our findings suggest that activation of CaSR promotes differentiation and adipogenesis in adipocytes, which might be achieved by upregulating PPARγ and its downstream gene expressions. Therefore, CaSR in adipocytes may be involved in the pathogenesis of obesity by promoting adipocyte differentiation and adipogenesis.