Objective To characterize the morphology and structure of hair surtaces and cross sections in children of different ages,and to understand the development and maturation processes and status of hairs in terms of ultrastructure in healthy children after birth.Methods Ninety healthy child volunteers were equally classified into 3 groups:infancy group (aged less than 1 year),toddler group (aged 1-2 years),and preschool-age group (aged 3-5 years).Hair samples were collected from the vertex,tempus,and occiput of each child.Field emission scanning electron microscopy was performed to obtain morphological data on surfaces and cross sections of hairs,including minimal and maximal hair diameters,hair index (the ratio of minimal to maximal diameter of hairs),hair cuticle thickness (measured on hair cross sections,each hair has 5-10 layers of cuticles),hair cuticle count (the number of cuticles per 100 μm length of hair shaft) and pattern (flat waveform or irregular shape).Statistical analysis was carried out by using analysis of variance,t test,chi-square test,and least significant difference (LSD) test with SPSS19.0 software.Results The minimal and maximal cross-sectional diameters of hairs significantly differed between the three age groups at the same anatomic site (vertex:F =39.67,28.32,respectively,both P ＜ 0.01;tempus:F =13.12,11.91,respectively,both P ＜ 0.01;occiput:F =18.41,16.43,respectively,both P ＜ 0.01),and increased with age,while no significant changes occurred in hair index with age.Meanwhile,significant differences were observed between the three age groups in the thickness of hair cuticles on the vertex (F =6.15,P ＜ 0.01),and in the number of hair cuticles on the vertex,tempus,and occiput (F=3.29,3.36 and 3.48 respectively,all P＜ 0.05).LSD test showed a significant difference in minimal and maximal hair diameters between the infancy group and toddler group and between the infancy group and preschool-age group (all P ＜ 0.001),an elevation in the thickness of hair cuticles on the vertex in the preschool-age group compared with the infancy group (0.59 ± 0.09 vs.0.49 ± 0.08 μm,P =0.003),but a decrease in the number of hair cuticles on the vertex,tempus and occiput in the preschool-age group compared with the infancy group (all P ＜ 0.05).No significant differences were observed between male and female volunteers in minimal and maximal hair diameters,hair index or hair cuticle thickness and number at the same anatomic site.In addition,there were no significant differences in the distribution of the two hair cuticle patterns between the three age groups.Conclusions Hair diameter and cuticle thickness both increase,while hair cuticle number decreases,with the development of children.

目的 检测尖锐湿疣(Condyloma Acuminatum,CA)患者皮损中FOXP3的表达情况,并探讨其在CA免疫学发病机制中的作用及其与HPV亚型的相关性.方法 采用免疫组织化学EnVision法检测FOXP3在CA皮损及正常包皮中的表达,同时应用基因芯片技术检测CA患者疣体脱落细胞中的HPV基因型.结果 CA患者皮损中FOXP3阳性率高于正常对照组(P＜0.01),且复发组高于初发组(P＜0.01)、长病程组高于短病程组(P＜0.01),但在低危型与高危型HPV感染组间差异无统计学意义(P＞0.05). 结论 CA患者皮损中FOXP3阳性率增高与CA的发生、发展关系密切,这种细胞免疫功能失调可能与其免疫学发病机制有关但与HPV亚型无关.

目的 观察窄谱中波紫外线(NB-UVB)联合卤米松局部外用治疗原发性皮肤淀粉样变病的临床疗效.方法 将入选的120例原发性皮肤淀粉样变病患者随机分为两组,治疗组62例,予窄谱中波紫外线联合卤米松局部外用;对照组58例,仅予局部外用卤米松治疗,在治疗第4,8和12周末时判定临床疗效.结果 治疗第4,8和12周末时,治疗组有效率分别为48.39％,61.29％和79.03％,对照组分别为39.66％,48.28％和58.62％,两组比较差异均有统计学意义(P均＜0.05).结论 窄谱中波紫外线联合卤米松局部外用治疗原发性皮肤淀粉样变病的疗效优于单用卤米松治疗.

颜面播散性粟粒性狼疮(Lupus Miliaris Disseminatus Faciei,LMDF)是一种发生在面部的慢性皮肤疾病,长期以来对其病因和发病机制尚不十分清楚,治疗上无特效疗法,一些研究提示本病某些细胞免疫检查显示异常.本组通过检测患者外周血T淋巴细胞核仁区嗜银蛋白染色(AgNORs),观察此病患者免疫功能状况及治疗前后免疫功能的比较.同时应用氨苯砜和转移因子联合治疗本病,观察其疗效,以寻求更有效的治疗方案.

Objective: To investigate the clinical efficacy and safety of combined topical therapy with 0.1% tacrolimus ointment and selenium sulfide lotion in the patients with seborrheic dermatitis of the scalp. Methods: Sixty-two patients were randomly divided into either 0.1% tacrolimus ointment group or halcinonide solution group, all the patients were topically applied twice daily with selenium sulfide lotion twice a week simultaneously. The course of treatment was 4 weeks, and the scores for clinical symptoms and signs were evaluated and recorded before and after treatment. Meanwhile the adverse events were also observed. Results: The effective rates of the two groups were 90.00% and 90.62% respectively, without significant difference(χ 2=0.12, P>0.05). One month after treatment, the relapse rate of tacrolimus group was 16.67%, whereas halcinonide group 40.62%(χ 2=4.31, P<0.05). Conclusion: Combined topical therapy of 0.1% tacrolimus ointment with selenium sulfide lotion is a safe and effective treatment for the seborreheic dermatitis of the scalp.

内分泌特别是各种性激素对毛发具有重要的调节作用,女性在妊娠期雌、孕激素大量增加,本文对这一时期女性头发的各个生长指标进行调查研究,为研究激素对头发特征的影响提供依据.

颜面播散性粟粒性狼疮(LMDF)是一种发生在面部的慢性皮肤疾病,长期以来对其病因和发病机制尚不十分清楚,治疗上无特效疗法,一些研究提示本病某些细胞免疫检查显示异常.本文拟从以下两方面探讨LMDF的病因及寻找有效治疗方案:①检测患者外周血T淋巴细胞核仁区嗜银蛋白染色(AgNORs),观察本病患者免疫功能状况及治疗前后免疫功能的比较.②应用氨苯砜和转移因子联合治疗本病,观察其疗效,以寻求本病更有效的治疗方案.

目的探讨转化生长因子β1(TGFβ1)在颜面播散性粟粒性狼疮(LMDF)发病中的作用. 方法采用免疫组化SP法检测20例LMDF皮损中TGFβ1,并以10例正常皮肤组织作对照.结果 LMDF组TGFβ1阳性率和着色程度均明显高于对照组(P<0.05). 结论 TGFβ1在LMDF的发病中可能起重要作用.

目的探讨细胞间黏附分子1（ICAM-1）在颜面粟粒性狼疮（LMF）发病中的作用。方法采用免疫组化SP法检测20例LMF皮损中ICAM-1的表达情况,并以10例正常皮肤组织作对照。结果LMF组ICAM-1表达阳性率和表达程度均明显高于对照组（P＜0.05）。结论ICAM-1的表达在LMF的发生、发展中起重要作用并提示LMF的发病与细胞免疫有关。

Objective To discuss the feasibility on morphology diagnosis of condyloma acuminata(CA) subclinc papillomavirus infection(SPI) by using electron colposcope observation.Methods ①Detect possible lesions in 96 patients of CA by using electron colposcope.②Assess the type of CA infection .Carry out acetic acid test and SP immunohistochemiscal assay to detect HPV infections in possible lesions.Results 268 possible lesions had been found in 96 patients.These 268 lesions could be divided into three major types named patch,spine and vascular.The positivity rate of acetic acid test was 72.8%(patch 96.5%,spine 90.5%,vascular 30.6%).The positivity rate was relatively low with vascular type as compared with the other two types(P0.05).The positive rate using the method of immunohistochemistry in HPV detection was 45.2%.There was no significant difference of three types(P0.05).Conclusion The electron colposcope observation is a more reliable method of detecting from a morphological aspect and diagnosing CA of subclinic papillomavirus infection than acetic acid test.

近来笔者应用氨苯砜(DDS)联合转移因子治疗20例颜面粟粒性狼疮(LMF)患者,获得满意疗效,现报告如下.