为了探索猪精液质量评价方法的相关性,对猪精子分别进行活力、PI染色、低渗肿胀试验、Rh123染色、FITC-PNA染色检查,统计其与体外受精的相关性.结果显示,各指标间存在较强的线性相关性,以顶体完整率与体外受精率的相关程度最高.说明顶体完整率在预测体外受精率方面更优于其他指标.

To investigate the ultrastructural change of cryopreserved Tibetan mastiff sperm,the random samples of both fresh and freeze-thaw sperms were examined by scanning electron microscope (SEM) and transmission electron microscope(TEM).The results showed that freeze preservation caused damage of the sperm structure to some extent,mainly resulting in the sperm being partly or totally fractured between head and neck,disappearance of the plasma membrane,vesiculation of the outer acrosomal membrane,indistinct or disappearing or zigzag annular ditch,swelled neck,and fuzzy mitochondrial structure.

以姜曲海种公猪冷冻精液为研究对象,观察了使用2种不同解冻方法对精液品质及体外受精胚胎发育的影响.试验结果表明,冷冻精液从液氮取出后,使用37℃水浴30s解冻在精子运动度、质膜完整率、顶体完整率和体外受精胚胎发育率方面均显著优于在室温放置20s后迅速投入37℃水浴10s解冻(P＜0.05).而与新鲜精液体外受精卵裂率和4细胞胚胎发育率相比,37℃水浴30s解冻方法与对照组无显著差异(P＞0.05).表明37.C水浴30s解冻方法优于室温放置20s后迅速投入37℃水浴10s解冻方法.