OBJECTIVE: To study the chemical constituents of Periploca calophylla. METHODS: The samples were separated and purified on silica gel, Sephadex LH-20, MCI, RP-18 column. Their structures were identified by spectral and chemical methods. RESULTS: Eight compounds were isolated from the chloroform part of P. calophylla, including periplocin (1), periplogenin (2), periplocoside M (3), 2α, 3β,23 trihydroxy ursolu 12 ene 28 carboxylic acid (4), glycoside E (5), ursolic acid (6),β-sitosterol (7), daucosterol(8). CONCLUSION: Compounds 2,3 and 4 are isolated from P. calophylla for the first time.