根据医学生物化学知识特点和教学大纲要求,甄选出适当内容制作直观动画,并在教学实践中应用.对动画在医学生物化学课堂教学中的应用效果进行评价分析,结果表明动画的应用不仅丰富了课堂教学内容,而且有助于学生对难点知识的理解与记忆,从而提高医学生物化学教学质量.

Objective:To identify the distribution characteristics of the single nucleotide polymorphisms(SNPs) of the human CYP1A2 gene in a Chinese Han ethnic healthy group in Wenzhou.Methods: PCR and direct DNA sequencing were used to detect all of seven exons and six introns of CYP1A2 gene in 108 peripheral blood specimens of healthy people.For the three detected polymorphic sites: 2159G>A,3613T>C and 5347C>T,PCR technique was further developed to investigate the frequency of genotypes and alleles in 472 healthy cases.Results:(1) 2159G>A site: the frequency of G and A alleles were 93.8% and 6.2%,respectively.The frequency of each genotype was 87.7%(GG),12.1%(GA),0.2%(AA)(χ2=0.325,P>0.05);(2) 3613T>C site: the frequency of T and C alleles were 97.9% and 2.3%,respectively.The frequency of each genotype was 95.3%(TT),4.4%(TC),0.3%(CC)(χ2=0.298,P>0.05);(3) 5347C>T site: the frequency of C and T alleles were 87.9% and 12.1%,respectively.The frequency of each genotype was 77.8%(CC),20.3%(CT),1.9%(TT)(χ2=0.742,P>0.05);(4) The frequency of the haplotype(2159 G>A,5347 C>T) was 3.2%.Conlusion: Three CYP1A2 SNPs,2159G>A,3613T>C,5347C>T and one haplotype(2159 G>A,5347 C>T) are present in Han ethnic healthy group in Wenzhou.