<span id="ChDivSummary" name="ChDivSummary" class="abstract-text"><正>中国是全球22个结核病高负担国家之一,每年新发生约133万结核病例[1],其中北京基因型结核分枝杆菌是在中国地区传播范围较广的菌株,>80%的结核分枝杆菌属于这个家族[2-4]。有研究显示,北京家族菌株与结核暴发、耐多药有关[5-7]。近年来,对北京家族菌株进行基因分型的散在重复单位分型法（mycobacterial interspersed repetitive units,MIRU）以其操作简便、结果数字化等优势得到越来越多的关注和研究。本研究应用多重PCR(de-</span>
Objective To explore the drug resistance level of Mycobacterium tuberculosis(MTB),and to provide evidence for the control of tuberculosis(TB).Methods Proportion method was used for drug susceptibility testing of isoniazid(INH),rifampicin(RFP),streptomycin(SM) and ethambutol(EMB) according to the WHO guidelines for Surveillance of Drug Resistance in Tuberculosis.ResultsThe resistant rate to any drug was 30.77% in all MTB isolates,according to cases of 299 sputum culture of Mycobacterium tuberculosis,27.5% in 262 new cases and 54.1% in 37 previously treated cases,respectively.Multi-drug resistant rate(MDR) was 6.7%.Initial and acquired MDR was 4.2% and 24.3%,respectively.Drug resistance rate of INH,SM,RFP,and EMB from high to low was 19.4%,16.1%,9.4% and 6.0%,respectively.The drug resistance rate did not show significant difference among different genders,age and domicile groups(P>0.05).Conclusion The results show that the situation of TB drug-resistance is severe in Yinzhou district of Ningbo City.The prevention and control of TB should be strengthened.
