目的 探讨乳腺癌组织中甲状腺激素受体β1(TRβ1)蛋白表达变化及其临床意义.方法 择取120例乳腺癌患者手术切除的乳腺癌组织标本及其癌旁组织标本.采用免疫组化SP法检测TRβ1蛋白表达.分析癌组织TRβ1蛋白表达与患者年龄、肿瘤直径、病理分级、临床分期、淋巴结转移及雌激素受体(ER)、孕激素受体(PR)、人类表皮生长因子受体2(HER-2)表达的关系.结果 癌组织及癌旁组织TRβ1蛋白阳性表达率分别为38.33%(46/120)、99.17%(119/120),二者比较P<0.05.癌组织TRβ1表达与患者临床分期和淋巴结转移有关(P均<0.05),与患者年龄、肿瘤直径、病理分级及ER、PR、HER-2表达情况无关(P均>0.05).结论 乳腺癌组织中TRβ1蛋白表达上调,检测乳腺癌组织中TRβ1蛋白表达有助于判断患者临床分期及预测淋巴结转移.

Objective:To assess clinical significance of hepatocellular apoptosis in polycystic liver disease(PCLD).Methods:Cell samples of cyst wall and normal liver were collected,and the cell apoptosis was observed using TUNEL in 43 patients with PCLD.The expression of apoptosis protein Fas,Caspase-3 and Bcl-2 were detected by immunohistochemistry.Results:TUNEL staining disclosed apoptosis index(AI) in cyst was significantly higher than in normal liver tissue.The expression of Fas and Caspase-3 in cyst wall cell was significantly higher than in normal liver tissue,contrary to Bcl-2.There no was a relationship among Tunel expression and age,gender,family history,symptom,albumin and GPT level;but the expression of Fas was positively correlated to AI in cyst.The expression of Fas associated with extrahepatic cyst but no relation to age,gender,family history,symptom,albumin and GPT level.Conclusions:Hepatocellular apoptosis plays an important role in pathogenesis of PCLD.Expression of Fas is a important to indicate whether patients with PCLD have extrahepatic cyst.

目的:分析手足口病的流行规律,寻找预防和控制该病流行的措施。方法:应用流行病学描述方法,对青海油田发生该病的幼儿园和小学进行调查。结果:此次疫情流行历时8天。共出现幼儿病例10人,病例集中在3个班。调查发现接诊医生不熟悉手足口病的临床表现,不能准确地筛查病例,基层人员缺乏手足口病防治知识。结论:幼儿园及小学应提高对手足口病的认识,严格执行消毒隔离制度、晨检筛查制度及疫情报告制度。

目的:了解超光谱β-内酰胺酶(ES-BLs)在肠杆菌科中流行情况。方法:用纸片确证法对临床送检标本进行ESBLs检测。结果:442株肠杆菌科细菌产ESBLs 127株,其中门诊标本产ESBLs有32株,检出率为19.5%,住院标本产ES-BLs有95株,检出率为34.2%,检出的这些阳性菌几乎对所有β-内酰胺类抗生素耐药;对氨基糖甙类、氟诺喹酮类部分耐药;对亚胺培南全部敏感。结论:革兰氏阴性菌中超光谱β-内酰胺酶的检测应作为临床微生物实验室的常规应用项目,以利于指导临床用药。