OBJECTIVE To establish a method to determine quercetin,podophyllotoxin and kaempferol in Diphylleia sinensis Li.METHODS Diamonsil C18 column was used.Methanol-0.4% glacial acetic acid solution was used as the mobile phaseat a flow rate of 1 mL·min-1.The column temperature was 30 ℃.The detection wavelength was 290 nm.RESULTS The liner ranges of quercetin,podophyllotoxin and kaempferol were 0.023-0.287 μg(r=0.999 9),0.208-2.6 μg(r=0.999 9),and 0.036 1-0.451 μg(r=0.999 9),respectively.The average recoveries were 99.64%(RSD=0.83%),100.04%(RSD=0.61%),and 99.88%(RSD=1.19%),respectively.CONCLUSION The method is sensitive,accurate and reproducible.It can be used for the determination of quercetin,podophyllotoxin and kaempferol in Diphylleia sinensis Li.

目的:测定不同产地窝儿七中槲皮素、鬼臼毒素、山奈素的质量浓度。方法:采用DiamonsilC18(4.6 mm×250 mm,5μm)色谱柱,甲醇-0.4%的冰醋酸水溶液为流动相,梯度洗脱;检测波长:290 nm,流速:1.0 mL/min,柱温30℃。结果:不同产地的窝儿七中槲皮素、鬼臼毒素、山奈素的差异较大,其中,眉县红河谷石海产窝儿七中槲皮素的质量浓度最高,为11.50 mg/g;太白县产窝儿七中鬼臼毒素的质量浓度最高,为106.69 mg/g;甘肃天水产窝儿七中山奈素的质量浓度最高,为8.36 mg/g。结论:产地对窝儿七中槲皮素、鬼臼毒素、山奈素有一定的影响。