In this study, through the construction of eukaryotic expressive vector, the recombinant Plasmids pEGFP-N3-vMIP-Ⅰ was transfected into Jurkat cells by electroporation. Then, by QRT-PCR technique, we detected the expression levels of anti-HIV genes: CCL5, APOBEC3F, MX1 in Jurkat cells which influenced by vMIP-Ⅰgene and explored the mechanisms of vMIP-Ⅰ against HIV infection. The results he recombinant plasmids of pEGFP-N3-vMIP-Ⅰwas successfully constructed and electroporation transfection efficiency reached about 40%. In comparison with non-transfected gourp, the transfected vMIP-Ⅰgroup can increase CCL5, A3G, A3F, and MX1 of Jurkat cells by 7.37, 1.58, 2.42 and 2.064 times seperately. The results suggest vMIP-Ⅰcan activate expressions of some relative anti-HIV genes in Jurkat cells, which probably is one of the mechanisms for anti-HIV infection.

Objective:To study the expression level of anti-HIV genes in KSHV (Kaposi’s sarcoma-associated herpesvirus) infected Jurkat cells.Methods: BCBL-1 cell was been stimulated by TPA (20ng/ml) 72 hours before havest KSHV virions solution.Jurkat cells were cultured with PRMI-1640 contain KSHV virions.24 hours after infection,The mRNA quantity of some anti-HIV genes in Jurkat cell infected with KSHV after 3 and 5 day’s were investigated by using Real-Time PCR.Results: After Jurkat cell infected with KSHV for 3 days,the mRNA production of RANTES,APOBEC3G,APOBEC3F,MX1,and MX2 increased 123,3.12,1.18,2.75,4.36 folds,respectively.In 5 days after infection,RANTES,MX1,and MX2 increased 11.91,2.72 and 2.22 times,respectively.Conclusions: the expression of anti-HIV genes(RANTES,APOBEC3G,APOBEC3F,MX1,MX2) up-regulated in KSHV infected Jurkat cells.

RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes or interference homologous gene expression by DNA Methylation.Traditionally,RNA interference is also called "post-transcriptional gene silencing (PTGS)".However,recent studies have revealed that siRNA initiates the activation of methytransferases,which causes the methylation of the promotor and silencie gene expression at post-transcriptional level.RNA interference (RNAi) can regulate a variety of biological processes.Recent evidence supports the notion that both cellular and viral miRNA (vmiRNA) are able to modulate viral replication.There is also evidence that viruses like HIV-1 have evolved strategies to control the cell’s RNAi activity.So it is expected that RNA interference may effectively applied in HIV-1 infection prevention and AIDS treatment.This article reviews the current progress of studies on the mecha- nism and application of RNA interference in HIV-1infection.

Based on the practice of bilingual teaching of biochemistry,this paper analyzed the present situation,problems and solutions of bilingual teaching in China,which will be helpful for developing bilingual teaching of biochemistry in medical colleges or universities.

通过对科学史教育融入生物化学教学的探索,总结了采用的教学手段和达到的教学效果,有利于培养素质全面的军事医学科学人才.