阐述基层防控重大动物疫病的策略,以期有效控制基层动物性疫病,为稳定发展畜牧产业保驾护航.

为探究山东省滨州市畜禽粪污资源的空间分布现状及近年来畜禽粪污土地承载力变化情况,优化畜牧业发展格局,推动畜牧养殖业绿色发展,参照《土地承载力测算技术指南》中的相关计算方法,测算了2018年滨州市各县区的畜禽粪污养分供应量、农作物粪肥养分需求量以及畜禽粪污土地承载力,分析了滨州市近6年畜禽粪污土地承载力变化.结果表明,滨州市的种植业与畜牧业发展规模在空间上存在一定的错位,种植业发达的地区主要为惠民县、邹平市、沾化区,而畜牧业发达的地区则分布在阳信县、无棣县;2018年滨州市农作物粪肥氮、磷养分需求量分别为3.47万t和0.74万t,畜禽粪肥氮、磷的供给量分别为2.98万t和0.56万t,可见,该区域农作物粪肥养分需求量均高于畜禽粪污养分供给量.从时间上来看,近6年滨州市以氮、磷为基准的畜禽粪污土地承载力指数均呈持续下降趋势.结果表明,滨州市全市畜禽养殖发展空间较大,可适度增加畜禽养殖规模,但在农牧业生产布局和区域间协同上仍需重点关注.

丁酸梭菌是一种以丁酸、乳酸和乙酸为主要代谢产物的专性厌氧革兰氏阳性芽孢杆菌,具有耐高温、耐酸和耐多种抗生素等生物学特性,优点为安全、无残留、绿色环保、稳定性好等,且有改善动物肠道环境,提高营养物质消化吸收利用率,提高动物机体免疫力和抗病力,抗氧化等多种生物学功能,是一种具有广泛开发潜力和开发前景的新型绿色饲料添加剂.文章就丁酸梭菌的形态特征与生物学特性、生物学功能及其在养猪生产中的研究与应用现状进行综述,为丁酸梭菌更好地推广应用于养猪生产提供参考.

黄芪多糖是黄芪的主要活性成分,具有一定的药理活性和保健功能.近年来,黄芪多糖在猪饲料添加剂、猪疫苗免疫增强剂、猪病预防治疗药物等方面得到了深入研究和应用,其在养猪生产中进一步推广应用将有望解决抗生素大量滥用导致的系列负面问题.文章综述了黄芪多糖在养猪生产方面的生物学功能与研究应用现状,探讨其在养猪业中的应用前景与发展趋势,以期为黄芪多糖在生猪养殖业中的进一步合理开发和推广应用提供参考依据.

自2011年以来,我国猪场陆续发生由PRV变异株引起的PR疫情,变异毒株与以往流行的经典毒株相比其毒力与致病性均显著增强,给我国养猪业造成了巨大的经济损失.文章对PRV变异毒株的流行特点、与疫苗株的交叉保护性、疫苗研究进展、防控措施进行阐述,以期为我国PR的净化提供参考.

微生态制剂是一类利用有益微生物及其代谢产物制成的生物制剂,将其作为动物饲料添加剂有望彻底解决当前抗生素大量滥用导致的系列负面问题.文章就微生态制剂在养猪生产中的研究应用现状及发展趋势进行综述,以期为微生态制剂在养猪生产中的进一步合理开发和推广应用提供参考依据.

A new type of cephodexD microcarrier was used to culture ST cells for the classical swine fever virus(CSFV) reproduction,and the results were compared with those of conventional monolayer culture.At 72 h,up to 18.9 × 105 cells/mL in microcarrier suspension culture,the cell density was two times than a static monolayer cultivation process.Compared with the monolayer culture,the virus titer was up to 7.5× 10s RID/mL in microcarrier suspension culture,increased by 50％.In one production process,more than two batch of qualification CSFV product could be harvested compared with traditional culture method.The CSFV produced by the new microcarrier suspension culture technology could stimulate the specific neutralizing antibody in pigs,and the protective rate was 100％.Therefore,it possessed a satisfied immunogenicity.The new microcarrier suspension culture process is superior to the monolayer culture method with an important application value in the field of CSFV large-scale production.

为了SPF鸡技术事业长足发展,我们应该重视SPF鸡舍的"硬件"即屏障环境设施.如何利用好、管理好先进的净化设备,发挥出其优良的性能,是保证并提高SPF鸡舍净化质量和SPF鸡群生产能力的重要"工程".

SPF鸡群维持是高风险的工程，屏障设施的可靠性和安全性非常重要，在SPF鸡舍管理过程中，在努力维护保养的同时，要设置好净化设施运行链安全性的最后一道防线---故障报警，这是SPF鸡屏障环境现代化智能管理系统不可或缺的重要组成部分。

An HPLC-MS method has been developed and validated for the quantitative determination of milbemycin oxime (MBO) in dog plasma. The developed method has been then applied in in vivo clinical studies to obtain pharmacokinetics of MBO in blood after its oral administration. Samples were extracted using solid-phase extraction (SPE). Sample proteins were precipitated with acetonitrile (ACN) and sodium chloride (NaCl) and then diluted with methanol and water. Calibration standards were prepared by using plasma matrix and following the same SPE procedure. Chromatographic separation was performed on a Waters C18 packed column (3.5 μm particles diameter; 3 × 100 mm) with a C18 guard column (3.5 μm particles diameter; 3 × 20 mm). The mobile phase was an 85:15 (v/v) mixed solution of ACN and 5 mM ammonium acetate. The calibration curve was linear over a concentration range of 2.0-500 ng/mL with a limit of quantitation of 2.0 ng/mL. The oral administration of a pellet of 2.5 mg MBO produced blood concentrations ranging from 6.10 ± 0.92 to 78.81 ± 4.38 ng/mL within 6 h, with a terminal half-time of 11.66 ± 0.93 h. This study determined the suitability of the herein proposed method to investigate the pharmacokinetics of MBO after oral administration.

Ethnopharmacological relevance: Flos populi (male inflorescence of Populus tomentosa Carriere) has been traditionally used in East Asian countries for the treatment of various inflammatory diseases, strengthening the spleen and stomach, anti-rheumatic, anti-tumor and anti-diarrhoeal.Aim of the study: To evaluate the in vivo or in vitro anti-diarrhoeal and anti-microbial activity of Flos populi aqueous extract.Materials and methods: Acute toxicity of Flos populi aqueous extract (FPAE) was investigated. Castor oil-induced diarrhoea method was used to evaluate the anti-diarrhoeal activity, inhibition of defecation and diarrhoea were determined in mice, effects on castor oil-induced enteropooling, intestinal transit and intestinal fluid secretion in rats or mice. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of FPAE against strains of three clinical bacterial isolates and one reference strain were used to test the anti-microbial activity.Results: The FPAE reduced the frequency of diarrhoea episodes and decreased the propulsion of charcoal meal through the gastrointestinal tract in a dose dependent manner. FPAE (100-500 mg/kg, p.o.) produced dose-dependent and significant (P < 0.01) protection of mice against castor oil-induced diarrhoea. FPAE, dose-dependently and significantly (P < 0.01) delayed the onset of castor-oil induced diarrhoea, decreased the frequency of defecation, and reduced the severity of diarrhoea. Compared with control animals, FPAE, dose-dependently and significantly (P < 0.01) decreased the volume of castor oil-induced intestinal fluid secretion, and reduced the number, weight and wetness of faecal droppings. There was no deaths or abnormalities in behaviour seen in the acute toxicity test. The aqueous extract displayed anti-microbial effects to three species of bacteria in anti-microbial test.Conclusion: The findings of this study indicate that FPAE possesses anti-diarrhoeal property in rats and mice and confirm the ethnomedicinal use of Flos Populi as a valuable natural remedy for the treatment, management and/or control of diarrhoea. These results may support the fact that this plant is traditionally used to cure diarrhoea. Crown Copyright (C) 2013 Published by Elsevier Ireland Ltd. All rights reserved.

Calpain system played an important role in muscle protein hydrolysis and muscle tenderization after animal slaughter. Fatty acid binding proteins affected muscle tenderness,flavor and juiciness,and had high relevance to backfat thickness,marbling, tenderness and water capacity of meat traits through taking part in the transport and metabolism of fatty acids. Myogenic regulatory factors controled the fusion of muscle cell and formation of muscle fiber during the muscle differentiation process. Myostatin,a negative regulator for muscle growth,could affect lean meat percentage of animal and better meat quality traits. Several meat quality candidate genes in cattle including calpain system family,fatty acid binding protein gene,myogenic regulatory factor,and myostatin were summarized in order to find the way to improve the quantity and quality of meat and further improve the meat quality traits through marker-assisted selection.

<正>改革开放30多年来,我国畜牧业持续稳定发展,逐步成为农民增收的支柱产业。随着畜牧业的迅猛发展,作为为畜牧业保驾护航的兽药行业,也得到了长足的进步。专家分析,未来10年内我国兽药行业将处于快速发展期,被称为"朝阳行业"[1],已成为当前利润增长最快的行业之一。

How to purify the quality of SPF chicken house and save energy are perplexing SPF chicken field all the time.Based on the automatic refitting and research for barrier facilities of SPF chicken house,the purifying facilities and biological purification will aim for a more optimized and energy efficient running state.It is expected to promote the rapid development of purification technology in SPF chicken house of our country.

对山东省特种动物特别是毛皮动物和鹿的产业化发展进行了历史回顾和现状调研,分析了该行业存在的问题和前景,以期对特种动物养殖业的发展有一定指导意义。

<正>借助于黄河三角洲高效生态经济区建设和山东蓝色半岛经济区建设的国家战略契机,滨州市畜牧业取得了突飞猛进的发展,产业结构日趋合理,畜产品生产总量得到了大幅度增长,兽药、饲料、畜产品加工等相关产业快速发展,畜牧业综合生产能力明显提高,畜牧业已成为富裕农民、壮大地方财政、拉动地方经济发展的强

<正>肉鸭养殖经过10多年的快速发展,已经成为畜牧养殖中的重要板块,其年均增长率(约10%)远高于肉鸡,市场高峰期年出栏量更是超过了30亿只;但是相对于肉鸡,肉鸭科研乏力,经费投入不足,育种水平与国外相比仍有差距;营养及生理研究工作也刚刚起步,专用生物制品的开发速度以及疾病防

No-pollution Animal Products are welcomed by people nowadays.There are unnegligible problems during producing no-pollution animal products.This artical is mainly focus on the problems and countermeasures while developing no-pollution animal products.we should deve op no-pollution animal products from the aspects of building standard quality system,quality supervision system,fostering leading enterprises and popularizing the related techniques of no-pollution product.

<正>当前流行的猪高热综合症,成为养猪业第一死因,其来势之猛,传播之快,死亡率之高,经济损失之大,是令人揪心的,受害养殖户处于困惑、焦急、不安、惶恐之中,有些人急功近利,治疗防治中出现不少失误,现将有关猪高热病防治中出现的问题提出一些看法,希望引起注意。1误诊错判,措施偏离发热、便秘、尿黄、不食,精神萎靡、皮肤红斑是共有特征。有的还有咳喘,呼吸