目的:检测长链非编码RNA肌蛋白反义RNA1(musculin antisense RNA 1,MSC-AS1)在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织和细胞中的表达,研究其对肿瘤细胞增殖的影响并探索其机制.方法:采用qRT-PCR技术检测NSCLC肿瘤组织、癌旁组织、肺癌细胞及正常肺上皮细胞中MSC-AS1的表达;分析患者的临床病理资料与MSC-AS1表达的相关性.利用LipofectamineTM2000对肺癌细胞进行转染;MTT及克隆形成实验测定细胞的增殖能力;同时利用qRT-PCR测定细胞中miR-302c-3p及SSX2IP的表达变化.生物信息学方法预测MSC-AS1及miR-302c-3p的下游靶基因;双荧光素酶报告实验验证基因间的靶向结合关系.结果:MSC-AS1在肺癌组织及肺癌细胞(A549、SPC-A1和SK-MES-1)中较癌旁组织及正常肺上皮细胞BEAS-2B中表达升高(P<0.05);MSC-AS1的表达水平与癌肿TNM分期、肿瘤大小及淋巴结转移密切相关(P<0.01),并提示患者的预后不良(P<0.01);敲低MSC-AS1能够抑制肿瘤细胞的增殖(P<0.05).MSC-AS1通过miR-302c-3p/SSX2IP轴调控NSCLC细胞的增殖.结论:LncRNA MSC-AS1能够促进NSCLC细胞的增殖并可能成为潜在的治疗靶点.

The new T helper cell Th17 is different from the traditional T helper cells Th1 and Th2 in differentiation and function.IL-17 is the main cytokine of Th17 cells.Th17 cells is closely related to inflammation and autoimmune diseases.Here we review the biological characteristics of Th17 cells,Focusing on the pathogenesis of Th17 cells and cytokines in myasthenia gravis(MG) and experimental autoimmune myasthenia gravis(EAMG).Study on Th17 cells would contribute to understanding the pathogenesis of myasthenia gravis and searching for new therapeutic targets.