由受重金属镉污染严重的化工厂土壤中,筛选分离获得两株镉耐受性加强的菌株,菌体长1.0～2.0 μm,宽0.5～0.8 μm,单个细胞呈杆状,单菌落分别呈淡黄色与乳白色,经鉴定为Enterobacter sp.与Serratia sp..对所分离细菌的最适生长条件、镉离子最小抑制浓度及抗生素抗性进行了检测和优化,并进一步研究了硫化镉-巯基乙酸纳米颗粒与细菌的相互作用,发现纳米颗粒浓度在150 μg/mL时细菌增殖基本停滞;过氧化物酶和超氧化物歧化酶酶活测试显示纳米颗粒对酶抑制性较Cd2+更为强烈,细胞形态受到严重破坏.

Flavonoids have significant free radical scavenging,antioxidant,anti-aging effect.In this study,stearic acid and lecithin were used as the carrier.The method of emulsion evaporation-solidification at low temperature was used to prepare flavonoids-loaded solid lipid nanoparticles from broussonetia papyrifera.When the ratio between stearic acid and phosphatidylcholine was 75：20,the effect was the best when PEG400 was used as approach emulsifier and stabilizer.The encapsulation efficiency was 82.67% and the drug loading was 33.73%.The average particle size of SLN was 461.3nm.