BNYVV CP and BBSV CP gene were transformed into sugarbeet of diferent variety using agrobacterium tumfaciens-mediated method.From kanamycin resistance regenerate plant,DNA fregment were obtained by PCR detection,transgenic plant were preliminarily proved.

The paper dealt with the study on the peroxidase and esterase isozymes as well as change of soluble protein content in the process of direct shoots organogenesis and genetic transformation of sugerbeet in vitro by polyarclamide gel electrophoresis(PAGE).The results showed that peroxidase(POD),isoesterase(EST)isoenzyme and soluble protein content appeared the changes of enzyme zymogram number and colour shade by adding benzylaminopurine(6-BA)to the medium,which related with increase of metabolizing activity.And after genetic transformation,bands of the peroxidase and esterase isozymes and soluble protein came forth obviously colour darken and number increase,which do farther research to identify the resons that resistance to stress was enhanced or natural transgene.

Choosing vigorous sprouts explants and shoots which grow in the spring and autumn as explants,the explants were cultivated on the medium MS with different combination of hormone. The results are as follows: The growing ability of explants grown in the spring and autumn was obvious different. The propagation coefficient of shoots growing in the spring and autumn is 4.35 and 9.94 respectively, while which of sprouts explants is 6.94 and 2.28. The kinds of hormone and combination of hormone were changed with different season, the most suitable hormone combination of shoots growing in the autumn is 2.4-D 0.1mg/L+KT0.1mg/L,while which of sprouts explants is 6-BA0.25mg/L+NAA0.5mg/L, 2.4-D 1.0mg/L+KT0.25mg/L is the optimum hormone combination of sprouts explants in the summer and which of autumn is 6-BA0.25mg/L+NAA0.25mg/L. Shoots were rooted on the medium 1/3MS+IBA 0.1mg/L and the highest rate of rooting is 90.5%, the final survival rate of which was around 85%.