在油酸钠体系下,考察了Fe 3+ 、Fe 2+ 随着pH值的变化对白云石、高岭土、石英纯矿物浮选的影响,以及在最佳pH值条件下Fe 3+ 、Fe 2+ 添加量对矿物浮选的影响;并分析了Fe 3+ 、Fe 2+ 对Zeta电位的影响作用。结果表明,白云石在油酸钠体系中有较好的浮选特性,且条件因素影响不显著;高岭土在油酸钠溶液中浮选回收率最高可达80%,在Fe 3+ 、Fe 2+ 参与的强酸强碱环境下几乎没有可浮性,Fe 3+ 一定程度上活化了高岭土,而Fe2+则起到了抑制作用;石英的可浮性较差,加入Fe 3+ 、Fe 2+ 后经调节pH值均能使石英的最佳回收率达到90%以上;Zeta电位测试证实了浮选实验的结果,说明Zeta电位的变化与回收率存在一定的内在关系。

The influence of Fe3+ and Fe2+ on the flotation of dolomite ,kaolin and quartz with pH change was investigated in presence of sodium oleate .Moreover ,the effect of Fe3+ and Fe2+ dosage on mineral flo-tation under optimum pH condition was also studied .Meanwhile ,the effect of Fe3+ and Fe2+ on Zeta po-tential was analyzed .The results showed that dolomite had good flotation characteristics in sodium oleate system ,and the influence of experiments conditions was insignificant .The highest flotation recovery of ka-olin was 80% in sodium oleate solution .However ,the flotability was not observed in strong-acid and strong-base environments in presence of Fe3+ and Fe2+ .Fe3+ could activate the kaolin ,w hile Fe2+ played the inhibition effect .The flotability of quartz was poor ,but the highest recovery could be up to 90% after adding Fe3+ and Fe2+ and adjusting pH value .The Zeta potential results proved the results of flotation tests ,indicating that the change of Zeta potential was related to the recovery rate .

With Changyuan uplift almond tectonic zone in southern Songliao Basin as an example, the paper studies formation pressure and the stress and strain in the process of injection-production relationship. Results show that the stress and strain are directly related to injection-production pressure changes; the stress and strain of different strata have different way of responding to the formation pressure change, and the stress and strain of the same layer's respectively changing water injection pressure and change the well bottom hole flowing pressure caused by the formation pressure change the way of the response is also different. The rising of water injection pressure, in the study area II group and Mr I - Mr II mudstone layer formation pressure rise will lead to the increase of horizontal stress and shear strain increases. The well bottom hole flow pressure will cause Mr II group and Mr I - Mr II mudstone layer formation pressure increases, the increase of horizontal stress and the increase of shear strain and Mr I, II group - falling II mudstone layer shear strain. According to the above rules, setting reasonable injection-production pressure shear strain is lower strata to relieve casing shear deformation and the effective ways to break fault.

The wheat TaNADP-ME1 gene was responsive to drought,salt and low temperature. In this research we successfully constructed the plant overexpression vector pCAME1 by recombinant technology,transformed the callus which was induced from the mature embryo of rice by agrobacterium-mediated method,acquired resistant callus by hy-gromycin and transformed plant by differentiation and regeneration on media. The 20 transgenic rice plants were ac-quired by PCR identification. This research laid a foundation for identification the function of TaNADP-ME1 gene.

焉耆黑蘑菇(Agaricus gennadii (Chot.et Boud.)P.D.Orton)是新疆特有优质野生食用菌,但目前还未人工驯化栽培.利用组织分离法分离菌种,并将焉耆黑蘑菇驯化成功.研究结果表明:焉耆黑蘑菇可以在青草(870 g/kg)为主料,麸皮(100 g/kg)、石灰(30 g/kg)为辅料的培养料上正常生长发育,接种到子实体采收约70～90 d,1 a可栽培2次.

兰州湾脆蘑是新疆特有的野生优质食用菌,目前还未对其进行分类鉴定.通过PCR扩增兰州湾脆蘑组织分离菌株的核糖体转录间隔区片段,并对其进行测序分析,探究其生物学特性.结果表明,组织分离菌株与荷叶离褶伞(GenBank登录号JF908330.1)相似度为99％,认为是荷叶离褶伞变种.生物学特性试验表明,兰州湾脆蘑菌丝生长的最适碳氮源分别是红糖和麸皮,最适碳氮质量比为50∶1,最适pH为6,最适温度20～22.5℃.

NADP-dependent malic enzyme(NADP-ME)is a key enzyme in C4 photosynthesis,the objective is to construct the TaNADP-ME1 gene into prokaryotic expression vector,express fusion protein in E.coli and purify the fusion protein.The TaNADP-ME1 gene was constructed into expression vector pET32a by recombination technology,recombination plasmid was identified by digestion with restriction enzymes and PCR amplification,and transformed into BL21(DE3)pLysS by CaCl2 method,fusion protein was induced by IPTG and purified by Ni2+-NTA agarose column.This research successfully acquired the recombination vector pETE1,and TaNADP-ME1 gene was accurately expressed in BL21(DE3)pLysS,SDS-PAGE revealed that the molecular weight of purified fusion protein was about 80 kDa and successfully acquired the fusion protein.This study laid a good foundation for identification the gene function of TaNADP-ME1 gene.

The cultivation conditions of Pholiota squarrosa mycelium were optimized in order to enhance product efficiency and shorten cultivation cycle.The one-factor experimental design and Plackett-Burman design showed that the optimum carbon source was maltose,the optimum nitrogen source was yeast,the optimum pH was 5,and that the optimum temperature was 23 ℃.What was more,the main factors influencing mycelium growth were maltose,yeast and K2HPO4.The optimum medium for Pholiota squarrosa mycelium growth was identified as 35g/L maltose+1.5g/L yeast+0.5 g/L K2HPO4+0.5g/L KH2PO4+0.5g/L MgSO4by the experiments of the steepest ascent and orthogonal designs.On the optimum medium the colony diameter was increased from 6.11cm to 7.41cm.

Objective To determine the bergenin and catechin contents in the tissue culture seedlings,seed seedlings and wild seedlings of Bergenia crassifolia,which would lay the foundation for the evaluation of artificial planting of this medical herb.Methods The HPLC analysis was achieved by using a C18 reverse column and methanol-water as the mobile phase,with a flow rate of 1.0 ml.min-1 and detected by UV at 275nm.The contents of bergenin and catechin in the different parts of axial root,fibrous root and leaves of B.crassifolia.Results The contents of bergenin and catechin varies in different parts of,the content of bergenin is higher in axial root and fibrous root while the content of catechin is higher in leaves.However,there are no significant differences between tissue culture seedlings,seed seedlings and wild seedlings.Conclusion This study indicated that the effective components of the artificial planting seedlings could fulfill the reguirments of medical applications.

By using sedimentary microfacies DTM,the 3D space distribution characteristics of the oil sandbody in Zhangxi Region is dynamically analyzed,and the tectonic sedimentary evolution mechanism of hydrocarbon accumulation is microscopically researched.It is proposed that the oil sandbody begin to depose,as delta lobes,from internal fan,and gradually change to middle fan and outer fan from south to north.We analyze the relationship between sedimentary microfacies and the physical properties of the reservoir layer,the structure and component of sandbody,the distribution of remaining oil,and so on,and expect to provide a reference for forecasting the distributions of oil sandbody and residual oil.

天山雪莲[Saussurea involucrata(Kar.et Kir.) Sch.-Bip.],又称新疆雪莲、雪莲或雪莲花,为菊科(Compositae)风毛菊属(Saussurea DC.)多年生草本植物,主要分布在新疆天山、昆仑山、阿尔泰山和帕米尔高原,生于海拔2 400 ～4 000 m的石缝、砾石坡和湿润沙地上,通常需5～8a发育成熟并开花结果.天山雪莲是传统药用植物,具有温肾助阳、祛风胜湿和通经活血等功效,主治类风湿性关节炎、小腹冷痛和月经不调等.现代药理和临床研究结果显示:天山雪莲还具有延缓衰老的功效,并且在治疗脑动脉硬化、缺血性中风、抗菌消炎、抑制癌细胞增殖和抗氧化等领域都有重要的研发价值[1-4].

目的测定厚叶岩白菜组培苗、种子苗和野生苗不同部位中熊果苷,为人工种植厚叶岩白菜的质量评价提供科学依据。方法采用反相C18色谱柱,甲醇-水为流动相洗脱,对不同厚叶岩白菜苗的主根、须根和叶片等不同部位中的熊果苷进行HPLC分析比较。结果厚叶岩白菜不同部位的熊果苷量存在一定差异,叶片中熊果苷最高;但组培苗和种子苗与野生苗的主根与须根中熊果苷的量差别很大,组培苗主根和须根熊果苷质量分数分别7.18%、6.12%。结论本研究表明采用组培或种子人工种植的厚叶岩白菜其有效成分能够满足临床和美容行业应用的要求。

Magnesium in salt mud was extracted by carbon dioxide method.The factors affecting its transformation into light magnesium oxide and the main reaction kinetics were studied.The optimum conditions are as follows:solid-liquid ratio 1∶15,carbon dioxide aeration time 90 min,hydrolysis temperature 90 ℃,hydrolysis time 25 min.Under these conditions,the total magnesium extraction rate is 75%.The reaction order and activation energy of the reaction between magnesium hydroxide and carbon dioxide are obtained by differentiation method,which are 1.7 and 68.12 kJ/mol respectively.

Taking the leaves and seeds of Abutilon avicennae as materials,the factors affecting the isolation mesophyll protoplasts from Abutilon avicennae were studied.The results showed that the best experiment materials were the leaves from the sterile seeding of Abutilon avicennae;if digested the leaves with combination of the enzyme solution 0.5% cellulase+0.3% macerozyme for 12~14 hours,more better protoplasts will be gotten.In the DPD liquid medium,the cells grew better.

The wheat TaNADP-ME2 was not only responsive to light but also to drought,salt and low temperature.The recombinant plant overexpression vector was constructed,transformed into rice and acquired transgenic rice plant.The vector was constructed by recombinant technology.The agrobacterium was transformed by freeze-thaw method.The callus induced from the mature embryo of rice was transformed by agrobacterium-mediated method.The transgenic rice was identified by hygromycin and PCR method.The results revealed that recombinant plant overexpression vector pSUE2 has been constucted succeedingly and transformed into EHA105.The 16 transgenic rice positive plants were acquired by the PCR detection from HPT and TaNADP-ME2 gene.This research laid a foundation for identification the function of TaNADP-ME2 gene in enhancing water use efficiency in drought tolerance.

Taking root tip of Saussurea involucrata as material for routine chromosome preparation,we hope that these results can provide the basis for classification,genetic breeding and molecular biology research of Saussurea involucrata.The present study analyzed the difference of sampling time,pretreatment and hydrolysis time on routine chromosome preparation of Saussurea involucrata impact.1 000 randomly selected root tip cell were observed in each treatment group in order to compare the proportion of metaphase cells and karyotype analysis of suitable metaphase cells.The results showed that: good tissue culture chromosome preparation was achieved by best sampling time at 10:00,24 h pretreatment of liquid mixture of same volume ratio of 0.002 mol/L 8-hydroxyquinoline and 1 g/L colchicine at 0 ℃,and by 7 min hydrolyzation of 1 mol / L hydrochloric acid at 58 ℃.The karyotype analysis revealed that the karyotype of Carthamus tinctorius L.was 2 n=2 N=24=20 m+16 sm.Chromosome composition of relative length was 2 n=32=8 L+6 M2+ 6 M1+12 S,its index of As.k was 60.998%,the karyotype asymmetry belonged to 2B type,its karyotype was symmetrica1.This suggested that Saussurea involucrata is relatively primitive forms.

Our objective was to investigate the important biological characteristics of annual artificial planting Saussurea involucrate in low altitude region(567 m).We hope that these results can provide essential referential materials for the utilization and protection of this kind of resource.Experiment in the present research was carried out on the ecological adaptation of S.involucrate such as leaf number,leaf length,root length and single plant fresh weight of annual artificial plant in low altitude region.The characteristics of annual plant growth of S.involucrate in low altitude were that it grew slowly after being sowed 45 d,then became fast,and reached a moderate stable rate after 70 d.Its emergence rate could be improved by sowing with covered straw mat.Its growth had a great drought-enduring characteristic.Annual plant of S.involucrate could be cut twice,and its leaf length and leaf number could grow to the size of an original plant after cutting approximately in 20 d,but the plexus amplitude was significantly smaller.Its regeneration could be promoted by cutting,which was also helpful to avoid the high temperature weather and pest outbreaks.

The different sampling time,the methods of the pretreatment and dissociation in chromosome sectioning of apricot cultivar shushanggan were compared.1 000 top root cells were observed,the proportions of metaphase cells and the metaphase cells which were suitable for karyotype analysis were compared.The results showed that the best experimental protocol for karyotype analysis of apricot cultivar shushanggan was the better sampling time at 8:30,the better pretreatment with 0.002 mol/L 8-Hydroxyquinolin for 4 h.The karyotype analysis revealed that the karyotype of apricot cultivar shushanggan was 2n=2N=16m and its index of As.k was 53.66%,the karyotype asymmetry belonged to 4A type,its karyotype was symmetrica1,which suggested that it was relatively primitive forms.

[Objective] To establish a rapid DNA extraction method for the screening of transgenic rice.[Method] The kit method and fast method of two DNA extraction methods were compared,and were proved by Ta NADP-ME1 and Ta NADP-ME2 gene transgenic rice respectively.[Result] PCR was done by using the DNA which extracted by the rapid method as template,about 1 700 bp Ta NADP-ME2 gene and about 1 900 bp Ta NADP-ME1 gene were obtained respectively,and the strips were clear and bright,comparing with the kit method showed no significant difference.[Conclusion] The rapid DNA extraction method of this study is economic,simple and quick,suitable for the large transgenic samples detection.

A circulation mass transfer method was used to prepare Na-montmorillonite by exchanging Ca with Na onto Ca-montmorillonite in high concentration sodium chloride solution.The influences of factors on ENa+ were investigated.Alkaline coefficient above 1 of Na-montmorillonite with excellent water swelling property was obtained under these conditions: the minimum concentration of NaCl was 3 %(mass);the concentration of slurry could reach to 25%;time for Na-Ca exchanging was more than 45 min including 15 min stirring time;NaCl should be added into deionized water before montmorillonite.A mathematical model was established which was between circulation times of mother liquor and concentration of initial sodium chloride,quantity of Ca-bentonite,cation exchanging Ca2+ E1/2Ca2+.