Diclazuril, a benzeneacetonitrile anticoccidial agent, has potent activity against various stages of Eimeria tenella (E. tenella). To study the effects of diclazuril on E. tenella merozoites, purified second-generation merozoites were obtained from infected chicken caecal tissue at 120h after inoculation by a combination of enzymatic digestion, centrifugation, erythrocytes disruption and percoll density gradient centrifugation. Ultrastructural changes were monitored by transmission electron microscopy (TEM). Apoptosis and mitochondrial transmembrane potential were determined by flow cytometry (FCM). The results showed that diclazuril induced ultrastructural changes and significantly increased the ratio of early apoptosis by 180.75% (P<0.01) and late apoptosis/necrosis by 86.82% (P<0.05) in second-generation merozoites, respectively. Compared with the infected/control group, the ratio of second-generation merozoites that lost mitochondrial function was increased by 45.04% (P<0.01) in the infected/treatment group. In conclusion, diclazuril induced morphological changes and attenuated the activity of mitochondrial transmembrane potential of merozoites, which is involved in mitochondrial-depended apoptosis in second-generation merozoites of E. tenella.

通过给小鼠灌服大黄水煎液的方法制作脾虚模型,然后比较了不同中药复方对脾虚小鼠的耐寒时间、脏器指数及体重的影响.结果发现复方二组较之脾虚模型组的小鼠耐寒时间长,脏器指数高.不同剂量的复方二对利血平致脾虚小鼠的试验结果表明,复方二能够极显著的抑制小肠的运动的亢进(P<0.01),提高胸腺指数(P<0.01)和血清淀粉酶活力,并对脾脏指数有一定的提高.

Astragalus polysaccharide has various physiology function such as enhance immunity, anti-cancer, antivirus, antioxidation, anti-aging and so on. It can impact the immune organs development of thymus, spleen, bursa of fabricius, promote transformation and proliferation of lymphocyte, phagocytosis of phagocytic cell, enhance cellular immunity and humoral immunity of animal organism. Recent advance in studies on pharmacological activities and application present situation on animal production of astragalus polysaccharides were reviewed in this paper.

介绍了有机胂制剂在畜禽生产中的作用、机理、毒性和残留.提出了由于其使用不当,通过其粪便进入环境中时对土壤和农作物产生一定危害.简单叙述了食品中有机胂的检测方法.